Abstract
A genetic selection in Saccharomyces cerevisiae for mutants that stimulate the mating pathway uncovered a mutant that had a hyperactive pheromone response pathway and also had hyperpolarized growth. Cloning and segregation analysis demonstrated that BUD14 was the affected gene. Disruption of BUD14 in wild-type cells caused mild stimulation of pheromone response pathway reporters, an increase in sensitivity to mating factor, and a hyperelongated shmoo morphology. The bud14 mutant also had hyperfilamentous growth. Consistent with a role in the control of cell polarity, a Bud14p-green fluorescent protein fusion was localized to sites of polarized growth in the cell. Bud14p shared morphogenetic functions with the Ste20p and Bni1p proteins as well as with the type 1 phosphatase Glc7p. The genetic interactions between BUD14 and GLC7 suggested a role for Glc7p in filamentous growth, and Glc7p was found to have a positive function in filamentous growth in yeast.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Cell Division
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DNA Mutational Analysis
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Fluorescent Antibody Technique, Indirect
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Genotype
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Green Fluorescent Proteins
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Intracellular Signaling Peptides and Proteins
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Luminescent Proteins / metabolism
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MAP Kinase Kinase Kinases
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Mating Factor
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Microfilament Proteins / metabolism
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Mutation
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Peptides / metabolism
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Phosphoprotein Phosphatases / metabolism*
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Plasmids / metabolism
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Protein Binding
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Protein Phosphatase 1
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Protein Serine-Threonine Kinases / metabolism
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Recombinant Fusion Proteins / metabolism
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Saccharomyces cerevisiae / enzymology
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Saccharomyces cerevisiae / metabolism*
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Saccharomyces cerevisiae Proteins / metabolism*
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Saccharomyces cerevisiae Proteins / physiology*
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Time Factors
Substances
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Bni1 protein, S cerevisiae
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Bud14 protein, S cerevisiae
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Intracellular Signaling Peptides and Proteins
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Luminescent Proteins
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Microfilament Proteins
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Peptides
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Recombinant Fusion Proteins
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Saccharomyces cerevisiae Proteins
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Green Fluorescent Proteins
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Mating Factor
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Protein Serine-Threonine Kinases
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MAP Kinase Kinase Kinases
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STE20 protein, S cerevisiae
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GLC7 protein, S cerevisiae
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Phosphoprotein Phosphatases
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Protein Phosphatase 1