Protein Overview: DRS1

General Information:
Name(s) found:	DRS1 / YLL008W [SGD]
Description(s) found: Found 23 descriptions. SHOW ALL	Nucleolar DEAD-box protein required for ribosome assembly and function, including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles [SGD]
Organism:	Saccharomyces cerevisiae
Length:	752 amino acids
Gene Ontology:
Cellular Component:	nuclear outer membrane [TAS] nucleolus [TAS]
Biological Process:	ribosome biogenesis [RCA] ribosomal large subunit assembly [TAS]
Molecular Function:	ATP-dependent RNA helicase activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MVVGTKKYSN LDFVPTISDS EDDVPILDSS DDEKVEAKKT TKKRKGKNNK KKVSEGDNLD  60
   61 EDVHEDLDAG FKFDLDADDT TSNFQGWNFL AEGESNKDDA EAFVKKDVDL DKIIRRKGGL 120
  121 VKMAHIDSKQ EEETEKEKVE KENDSDDEEL AMDGFGMGAP MNNGDENQSE EEEEEEEKEE 180
  181 EEEEEEEQEE MTLEKGGKDD EIDEEDDSEE AKADFYAPET EGDEAKKQMY ENFNSLSLSR 240
  241 PVLKGLASLG YVKPSPIQSA TIPIALLGKD IIAGAVTGSG KTAAFMIPII ERLLYKPAKI 300
  301 ASTRVIVLLP TRELAIQVAD VGKQIARFVS GITFGLAVGG LNLRQQEQML KSRPDIVIAT 360
  361 PGRFIDHIRN SASFNVDSVE ILVMDEADRM LEEGFQDELN EIMGLLPSNR QNLLFSATMN 420
  421 SKIKSLVSLS LKKPVRIMID PPKKAATKLT QEFVRIRKRD HLKPALLFNL IRKLDPTGQK 480
  481 RIVVFVARKE TAHRLRIIMG LLGMSVGELH GSLTQEQRLD SVNKFKNLEV PVLICTDLAS 540
  541 RGLDIPKIEV VINYDMPKSY EIYLHRVGRT ARAGREGRSV TFVGESSQDR SIVRAAIKSV 600
  601 EENKSLTQGK ALGRNVDWVQ IEETNKLVES MNDTIEDILV EEKEEKEILR AEMQLRKGEN 660
  661 MLKHKKEIQA RPRRTWFQSE SDKKNSKVLG ALSRNKKVTN SKKRKREEAK ADGNGARSYR 720
  721 KTKTDRIADQ ERTFKKQKST NSNKKKGFKS RR

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ARX1 BRX1 CIC1 DBP10 DBP9 DRS1 EBP2 ERB1 HAS1 LOC1 MAK21 MAK5 MDN1 MRT4 NIP7 NOC2 NOC3 NOG1 NOP15 NOP2 NOP4 NOP7 NSA1 NSA2 NUG1 PUF6 RIX1 RIX7 RLP7 RPF1 RPF2 RPL25 RRP1 RRP12 SDA1 SPB1 SPB4 TIF6 URB1 YTM1
View Details	(MIPS) Mewes HW, et al. (2004)		DRS1 GAR1 IFH1 NOP1 NPL3 NSR1 POP1 POP2 RAT1 RNT1 RRP3 SBP1 SNM1 SRD1 UTP10 UTP11 UTP13 UTP4 UTP5 UTP6 UTP7 UTP8 UTP9 YME2
View Details	Krogan NJ, et al. (2006)		DRS1 ERB1 HAA1 HRQ1 MKT1 STE20 UBP1
View Details	Gavin AC, et al. (2002)		AAR2 ARX1 BEM2 BMS1 BRX1 BUD20 CBF5 CIC1 CLU1 CYR1 CYS4 DBP10 DBP9 DCP2 DRS1 EBP2 ERB1 FPR4 FUN12 GAR1 GCN1 HAS1 HDA1 HER1 HIS3 INP2 IPI1 IPI3 KRE33 KRR1 LHP1 LOC1 LSG1 MAK21 MAK5 MDN1 MIS1 MRP7 MRT4 NAP1 NHP2 NIP1 NIP7 NMD3 NOC2 NOC3 NOG1 NOG2 NOP1 NOP12 NOP15 NOP16 NOP2 NOP4 NOP56 NOP6 NOP7 NSA1 NSA2 NUG1 POL2 PRP4 PRT1 PUF6 REI1 RIX1 RIX7 RLP24 RLP7 RPA135 RPA190 RPF1 RPF2 RPG1 RPP2B RPT6 RRP1 RRP12 RSA3 RSA4 RTR1 SDA1 SEC7 SKI2 SMB1 SNU114 SNU13 SNU66 SPB1 SPB4 SRO9 SSF1 TIF4631 TIF4632 TIF6 TUB3 URB1 UTP10 UTP20 UTP21 UTP22 YGL036W YMR310C YTM1
View Details	Ho Y, et al. (2002)		CBF5 CBP2 CIC1 DBP7 DRS1 EBP2 ENP2 ERB1 HTB1 IMD2 IMD3 KAP95 KRE33 KRI1 KRR1 MNP1 MSS116 NOP13 NOP2 NOP58 NOP6 NOP7 NUG1 PUF6 PWP1 RPF2 RRP5 TIF1, TIF2 TIF4631 TIF6 TSR1 URB2
View Details	Qiu et al. (2008)		AIR1 BFR2 BUD22 CIC1 DBP10 DBP3 DBP6 DBP7 DBP8 DBP9 DHR2 DRS1 ECM16 ESF1 FAL1 FCF2 GAR1 HAS1 HCA4 IFH1 KRR1 MAK5 MDN1 MTR4 NOC3 NOG2 NOP1 NOP12 NOP13 NOP15 NOP4 NOP7 NOP8 NPL3 NSR1 NUG1 POP1 PRP43 PUF6 PXR1 RAT1 RIX7 RNT1 ROK1 RPP1 RRB1 RRP1 RRP3 RRP5 RRP8 RRP9 SGD1 SNM1 SOF1 SPB4 SRD1 SSF1 UTP10 UTP13 UTP14 UTP21 UTP4 UTP6 UTP7 UTP8 UTP9

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	BOI1	Sample boi 1 with ha tag from october 2005	McCusker D, et al (2007)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#02 Alpha Factor Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	BUD14	DRS1	2	Unpublished Fields Lab Data
View Screen	THG1	DRS1	1	Hazbun TR, et al. (2003)

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	DRS1 and NOP56	Huh WK, et al. (2003)
View Data	DRS1	Huh WK, et al. (2003)
View Data	DRS1	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..68]	ORFEUS	9.57	No description for 1l8mA was found.
2	View Details	[69..147]	ORFEUS	9.57	No description for 1l8mA was found.
3	View Details	[148..231]	ORFEUS	9.57	No description for 1l8mA was found.
4	View Details	[232..444]	PSI-BLAST	572.228787	Putative DEAD box RNA helicase
5	View Details	[445..598]	PSI-BLAST	572.228787	Putative DEAD box RNA helicase
6	View Details	[599..752]	PSI-BLAST	12.0	Nucleotide excision repair enzyme UvrB

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
translation initiation factor activity	7.79457008386475	bayes_pls_golite062009
translation factor activity, nucleic acid binding	4.98637553020315	bayes_pls_golite062009
translation regulator activity	4.94658029227343	bayes_pls_golite062009
	4.704140727858	bayes_pls_golite062009
structural constituent of ribosome	4.56509805974548	bayes_pls_golite062009
structural molecule activity	3.58406348799895	bayes_pls_golite062009
nucleoside-triphosphatase activity	2.95079405147356	bayes_pls_golite062009
pyrophosphatase activity	2.87427509025394	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	2.85499564954033	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	2.85178667290149	bayes_pls_golite062009
motor activity	2.79327761685782	bayes_pls_golite062009
microtubule motor activity	2.74365461017633	bayes_pls_golite062009
binding	2.64537357529343	bayes_pls_golite062009
transporter activity	2.58140740499725	bayes_pls_golite062009
hydrolase activity	2.55157966585974	bayes_pls_golite062009
nucleic acid binding	2.51683451033161	bayes_pls_golite062009
transmembrane transporter activity	2.39695871109024	bayes_pls_golite062009
ATPase activity	2.39535714892224	bayes_pls_golite062009
RNA binding	2.29590582680214	bayes_pls_golite062009
ATPase activity, coupled	2.18396165590908	bayes_pls_golite062009
RNA cap binding	2.17871969124431	bayes_pls_golite062009
DNA binding	1.94055378599526	bayes_pls_golite062009
transcription regulator activity	1.83584389083042	bayes_pls_golite062009
helicase activity	1.70242731830224	bayes_pls_golite062009
protein-DNA loading ATPase activity	1.54436515966471	bayes_pls_golite062009
DNA clamp loader activity	1.51134988628578	bayes_pls_golite062009
RNA helicase activity	1.42791449104435	bayes_pls_golite062009
cytoskeletal protein binding	1.40488517180956	bayes_pls_golite062009
RNA-dependent ATPase activity	1.39335733374328	bayes_pls_golite062009
ATP-dependent RNA helicase activity	1.36724390116598	bayes_pls_golite062009
substrate-specific transporter activity	1.29730375126024	bayes_pls_golite062009
DNA-dependent ATPase activity	1.2155186842031	bayes_pls_golite062009
purine NTP-dependent helicase activity	1.19311444180077	bayes_pls_golite062009
ATP-dependent helicase activity	1.19311444180077	bayes_pls_golite062009
catalytic activity	1.00104173493322	bayes_pls_golite062009
tubulin binding	0.979946830272106	bayes_pls_golite062009
DNA-directed DNA polymerase activity	0.971476020912368	bayes_pls_golite062009
transcription factor activity	0.959949409533517	bayes_pls_golite062009
purine nucleotide binding	0.929901285538132	bayes_pls_golite062009
DNA polymerase activity	0.928091843881235	bayes_pls_golite062009
nucleotide binding	0.922442819194247	bayes_pls_golite062009
purine ribonucleotide binding	0.920987930180051	bayes_pls_golite062009
ribonucleotide binding	0.920926158369519	bayes_pls_golite062009
microtubule binding	0.907162506177187	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	0.906554174337376	bayes_pls_golite062009
actin binding	0.883285269827407	bayes_pls_golite062009
structure-specific DNA binding	0.782430219754236	bayes_pls_golite062009
mRNA binding	0.76699944561918	bayes_pls_golite062009
DNA helicase activity	0.72881469450553	bayes_pls_golite062009
ribonuclease activity	0.712344060953362	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	0.652333406433794	bayes_pls_golite062009
double-stranded DNA binding	0.6071273686445	bayes_pls_golite062009
transcription repressor activity	0.607045223341107	bayes_pls_golite062009
ATP-dependent DNA helicase activity	0.5886142102548	bayes_pls_golite062009
GTPase activity	0.437665353870877	bayes_pls_golite062009
active transmembrane transporter activity	0.434775387083003	bayes_pls_golite062009
snRNA binding	0.418985924846052	bayes_pls_golite062009
ATP binding	0.41092997612902	bayes_pls_golite062009
adenyl ribonucleotide binding	0.385813421701492	bayes_pls_golite062009
adenyl nucleotide binding	0.377870145186619	bayes_pls_golite062009
ion transmembrane transporter activity	0.355466224802969	bayes_pls_golite062009
guanyl nucleotide binding	0.296406132096546	bayes_pls_golite062009
GTP binding	0.295705904888768	bayes_pls_golite062009
primary active transmembrane transporter activity	0.294474570607914	bayes_pls_golite062009
guanyl ribonucleotide binding	0.29028316917561	bayes_pls_golite062009
P-P-bond-hydrolysis-driven transmembrane transporter activity	0.282352478532746	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances	0.220608508617331	bayes_pls_golite062009
ATPase activity, coupled to transmembrane movement of substances	0.219533217661421	bayes_pls_golite062009
ATPase activity, coupled to movement of substances	0.219157471322311	bayes_pls_golite062009
protein binding	0.170837278876362	bayes_pls_golite062009
microfilament motor activity	0.10236059675512	bayes_pls_golite062009
protein transporter activity	0.0397092337273124	bayes_pls_golite062009
cation transmembrane transporter activity	0.0254369227200564	bayes_pls_golite062009
signal sequence binding	6.53725193420218E-4	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.88

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2