Protein Overview: RPG1

General Information:
Name(s) found:	RPG1 / YBR079C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|974205\|emb\|CAA53937.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|6319553\|ref\|NP_009635.1\| Subunit of the core complex of translation initiation factor 3(eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes; Rpg1p [Saccharomyces cerevis [NCBI NR] pir\|\|S45944 hypothetical protein YBR079c - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|586516\|sp\|P38249.1\|EIF3A_YEAST RecName: Full=Eukaryotic translation initiation factor 3 subunit A; Short=eIF3a; AltName: Full=Eukaryotic translation initiation factor 3 110 kDa subunit homolog; Short=eIF3 p110; AltName: Full=Translation initiation fact [NCBI NR] gi\|536338\|emb\|CAA85024.1\| TIF32 [Saccharomyces cerevisiae] [NCBI NR] gi\|2580607\|gb\|AAB82416.1\| translation initiation factor 3 p110 subunit; Tif32p [Saccharomyces cerevisiae] [NCBI NR] Eukaryotic translation initiation factor 3 110 kDa subunit - Saccharomyces cerevisiae (Baker's yeast) [Swiss-Prot] Eukaryotic translation initiation factor 3 subunit A OS=Saccharomyces cerevisiae GN=TIF32 PE=1 SV=1 [Swiss-Prot] RPG1 Translation initiation factor eIF3 (p110 subunit) [mips] eIF3a subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes [SGD] RPG1, Chr II from 395341-398235, reverse complement [yeast_orfs2] Chr II from 395341-398235, reverse complement [yeastorf3.fasta] Chr II from 398233-395339, reverse complement [yeastorf5.fasta] (P38249) Eukaryotic translation initiation factor 3 110 kDa subunit (eIF3 p110) (Translation initiation factor eIF3, p110 subunit) (eIF3a) [4932.FASTACCERV.txt] RPG1 SGDID:S0000283, Chr II from 398234-395340, reverse complement, Verified ORF [yeastflysequest.fasta] RPG1 SGDID:S0000283, Chr II from 398271-395377, reverse complement, Verified ORF [10112005cervSGD.fasta] Rpg1p [Saccharomyces cerevisiae]gi\|536338\|emb\|CAA85024.1\| TIF32 [Saccharomyces cerevisiae]gi\|974205\|emb\|CAA53937.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|586516\|sp\|P38249\|IF3A_YEAST Eukaryotic translation initiation factor 3 110 kDa subu [nrdb_11152004_Chlamydomonas] RPG1 SGDID:S000000283, Chr II from 398271-395377, reverse complement, Verified ORF, "Subunit of the core complex of translation initiation factor 3(eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] translation initiation factor 3 p110 subunit; Tif32p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] TIF32 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Eukaryotic translation initiation factor 3 110 kDa subunit (eIF3 p110) (Translation initiation factor eIF3, p110 subunit) (eIF3a) [nr-271106-contam.fasta] Subunit of the core complex of translation initiation factor 3(eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes; Rpg1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] RPG1 SGDID:S000000283, Chr II from 398271-395377, reverse complement, Verified ORF, "eIF3a subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding o [SGD_S-cerevisiae_na_01-05-2010_reversed.fasta] RPG1 SGDID:S000000283, Chr II from 398271-395377, reverse complement, Verified ORF, "Subunit of the core complex of translation initiation factor 3(eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes" [yeast-bovrabprots.fasta] [chemostatdb.fasta] RPG1 SGDID:S000000283, Chr II from 398271-395377, reverse complement, Verified ORF, "eIF3a subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes" [SGDyeast.20101221.fasta] Eukaryotic translation initiation factor 3 subunit A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RPG1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] RPG1 SGDID:S000000283, Chr II from 398277-395383, Genome Release 64-1-1, reverse complement, Verified ORF, "eIF3a subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a Prt1p-Rpg1p-Nip1p subcomplex that stimulates binding of mRNA and tRNA(i)Met to ribosomes; involved in translation reinitiation" [yeast-030211-contam.fasta] RPG1 SGDID:S000000283, Chr II from 398277-395383, Genome Release 64-2-1, reverse complement, Verified ORF, "eIF3a subunit of the eukaryotic translation initiation factor 3 (eIF3); subunit of the core complex of eIF3; essential for translation; part of a Prt1p-Rpg1p-Nip1p subcomplex that stimulates binding of mRNA and tRNA(i)Met to ribosomes; involved in translation reinitiation" [20141208_orf_trans.fasta] YBR079C SGDID:S000000283, Chr II from 398277-395383, Genome Release 64-1-1, reverse complement, Verified ORF, "eIF3a subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a Prt1p-Rpg1p-Nip1p subcomplex that stimulates binding of mRNA and tRNA(i)Met to ribosomes; involved in translation reinitiation" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	964 amino acids
Gene Ontology:
Cellular Component:	incipient cellular bud site [IDA] cytoplasm [IDA] ribosome [TAS] eukaryotic translation initiation factor 3 complex [IDA][IPI ]
Biological Process:	translational initiation [IDA][TAS]
Molecular Function:	translation initiation factor activity [IDA][TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MAPPPFRPEN AIKRADELIS VGEKQAALQS LHDFITARRI RWATPSTVEP VVFKFLEIGV  60
   61 ELKKGKLLKD GLHQYKKLIQ GSTEGLVSVG AVARKFIDLV ESKIASEQTR ADELQKQEID 120
  121 DDLEGGVTPE NLLISVYESD QSVAGFNDEA ITSWLRFTWE SYRAVLDLLR NNALLEITYS 180
  181 GVVKKTMHFC LKYQRKNEFK RLAEMLRQHL DAANYQQSKS GNNLVDLSDA DTLQRYLDQR 240
  241 FQQVDVSVKL ELWHEAYRSI EDVFHLMKIS KRAPKPSTLA NYYENLVKVF FVSGDPLLHT 300
  301 TAWKKFYKLY STNPRATEEE FKTYSSTIFL SAISTQLDEI PSIGYDPHLR MYRLLNLDAK 360
  361 PTRKEMLQSI IEDESIYGKV DEELKELYDI IEVNFDVDTV KQQLENLLVK LSSKTYFSQY 420
  421 IAPLRDVIMR RVFVAASQKF TTVSQSELYK LATLPAPLDL SAWDIEKSLL QAAVEDYVSI 480
  481 TIDHESAKVT FAKDPFDIFA STASKEVSEE ENTEPEVQEE KEETDEALGP QETEDGEEKE 540
  541 EESDPVIIRN SYIHNKLLEL SNVLHDVDSF NNASYMEKVR IARETLIKKN KDDLEKISKI 600
  601 VDERVKRSQE QKQKHMEHAA LHAEQDAEVR QQRILEEKAA IEAKLEEEAH RRLIEKKKRE 660
  661 FEAIKEREIT KMITEVNAKG HVYIDPNEAK SLDLDTIKQV IIAEVSKNKS ELESRMEYAM 720
  721 KKLDHTERAL RKVELPLLQK EVDKLQETDT ANYEAMKKKI VDAAKAEYEA RMADRKNLVM 780
  781 VYDDYLKFKE HVSGTKESEL AAIRNQKKAE LEAAKKARIE EVRKRRYEEA IARRKEEIAN 840
  841 AERQKRAQEL AEATRKQREI EEAAAKKSTP YSFRAGNREP PSTPSTLPKA TVSPDKAKLD 900
  901 MIAQKQREME EAIEQRLAGR TAGGSSPATP ATPATPATPT PSSGPKKMTM AEKLRAKRLA 960
  961 KGGR

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		FUN12 GCD11 HCR1 NIP1 PRT1 RPG1 RPS8B, RPS8A SUI1 SUI2 TIF34 TIF35 TIF5 YEF3
View Details	(MIPS) Mewes HW, et al. (2004)		GCD10 NIP1 PRT1 RPG1 SUI1 TIF34 TIF35
View Details	Krogan NJ, et al. (2006)		ADE8 CHZ1 ECM2 HCR1 HEM12 KAP122 NIP1 PRT1 RPG1 SUI1 SYH1 TIF34 TIF35 TIF5 YOR052C
View Details	Gavin AC, et al. (2006)		FUN12 HCR1 NIP1 PRT1 RLI1 RPG1 TIF34 TIF35 TIF5
View Details	Gavin AC, et al. (2002)		ADA2 ADH1 ARX1 ASC1 ATG9 AVO1 BDF2 BEM2 BMS1 BRX1 BUD20 CAB3 CDC55 CEG1 CET1 CHD1 CIC1 CKA1 CKA2 CKB1 CKB2 CLU1 COG3 CSE2 CTR9 CYR1 CYS4 DBP10 DBP9 DCP2 DRS1 DYN1 EBP2 ECM5 ENO2 ERB1 FAB1 FBA1 FPR4 FUN12 GAL11 GCN1 GCN5 GLT1 GLY1 HAS1 HAT1 HAT2 HCR1 HDA1 HER1 HHF1, HHF2 HIF1 HIS3 HOT1 HSM3 ICL1 IML1 INP2 IPI1 IPI3 IST2 ISW1 ISW2 KAP123 KRE33 KTR5 LHP1 LIP2 LOC1 LSG1 LSM12 MAC1 MAK21 MAK5 MDN1 MED1 MED11 MED2 MED4 MED6 MED7 MED8 MIS1 MLP1 MRPL6 MRT4 MSB1 NAP1 NIP1 NIP7 NMD3 NMD5 NOC2 NOC3 NOG1 NOG2 NOP1 NOP12 NOP15 NOP16 NOP2 NOP4 NOP56 NOP7 NSA1 NSA2 NUG1 NUT1 NUT2 PDB1 PFK1 PGD1 PGK1 PRO1 PRP43 PRT1 PSD2 PSE1 PSH1 PUF6 REI1 RET1 RGR1 RIC1 RIX1 RIX7 RLI1 RLP24 RLP7 ROX3 RPA135 RPA190 RPC40 RPF1 RPF2 RPG1 RPN12 RPN3 RPN6 RPN8 RPN9 RPO21 RPP2B RPT2 RPT3 RPT5 RPT6 RRP1 RRP12 RSA3 RSA4 RSC3 RTR1 RVB2 SAM1 SDA1 SEC7 SIN4 SKI2 SNU13 SPB1 SPB4 SPT16 SPT5 SPT7 SRB2 SRB4 SRB5 SRB6 SRB7 SRB8 SRP1 SSA3 SSA4 SSE2 SSF1 SSN2 SSN3 SSN8 SUA7 SUI1 SUI2 SUI3 SUP45 TAF6 TEL1 TIF1, TIF2 TIF34 TIF35 TIF5 TIF6 TOP2 TSC11 TUB3 URB1 USO1 UTP10 UTP20 UTP22 UTP6 VPS1 VTH2 YFR006W YGL036W YIL077C YPR077C YTA7 YTM1
View Details	Ho Y, et al. (2002)		ATP3 BEM2 COP1 DUF1 EDE1 FOL2 HTB2 LHS1 NIP1 POR1 RPG1 SES1 SRV2 SUI1 TIF34 TIF35 UBI4
View Details	Qiu et al. (2008)		CLU1 FUN12 HCR1 NIP1 PRT1 RPG1 SUI3 TIF11 TIF3 TIF34 TIF35 TIF5

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	ALG13	No Comments	Hazbun TR, et al. (2003)
View Run	NAF1	No Comments	Hazbun TR, et al. (2003)
View Run	RIX1	No Comments	Hazbun TR, et al. (2003)
View Run	RBA50	No Comments	Hazbun TR, et al. (2003)
View Run	PGA1	No Comments	Hazbun TR, et al. (2003)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	BOI1	Sample boi 1 with ha tag from october 2005	McCusker D, et al (2007)
View Run	BOI1	Sample boi1 with gst from october 2005	McCusker D, et al (2007)
View Run	BOI1	Sample bob1 (2nd set) from october 2005	McCusker D, et al (2007)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#04 Alpha Factor Prep2	Keck JM, et al. (2011)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	RPG1	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..158]	ORFEUS	11.47	Transcription factor MalT domain III
2	View Details	[159..285]	ORFEUS	11.47	Transcription factor MalT domain III
3	View Details	[286..451]	ORFEUS	11.47	Transcription factor MalT domain III
4	View Details	[452..521]	deduced	N/A	Confident ab initio structure predictions are available.
5	View Details	[522..610]	PSI-BLAST	8.69897	Tropomyosin
6	View Details	[611..964]	PSI-BLAST	13.0	Heavy meromyosin subfragment

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Protein Overview: RPG1

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: