Protein Overview: TIF3

General Information:
Name(s) found:	TIF3 / YPR163C [SGD]
Description(s) found: Found 27 descriptions. SHOW ALL	Translation initiation factor eIF-4B, has RNA annealing activity; contains an RNA recognition motif and binds to single-stranded RNA [SGD]
Organism:	Saccharomyces cerevisiae
Length:	436 amino acids
Gene Ontology:
Cellular Component:	ribosome [TAS]
Biological Process:	translational initiation [TAS]
Molecular Function:	translation initiation factor activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MAPPKKTVKK MDLTSFLNDD TFGSSWAEED VDLNKITIPI ETANANTIPL SELAHAKNNS  60
   61 NNTRSGGFGG SFGGRSRLDP ALGGGSSDRR EEYPVPDAPP YRAVINNIPW DITPEGVQAW 120
  121 VEDGLVKPEA VEEVVLPKNL RDPTRLKGNA FVTLKERADL VAVLKFNGTK LNERTVYVSV 180
  181 AAPRRGGGAD VDWSSARGSN FQGDGREDAP DLDWGAARGS NFRGPRRERE EVDIDWTAAR 240
  241 GSNFQGSSRP PRREREEVDI DWSAARGSNF QGSSRPPRRE REEPDIDWSA ARGSNFQSSS 300
  301 RPPRREREEP DIDWSAARGS NFQSSSRPPR REREKEEPAL DWGAARGAQF GKPQQTKNTY 360
  361 KDRSLTNKKT TDEQPKIQKS VYDVLRTEDD DEDEEAEKQN GDAKENKVDA AVEKLQDKTA 420
  421 QLTVEDGDNW EVVGKK

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Krogan NJ, et al. (2006)		BYE1 MLP2 NOC4 RIF1 TIF3
View Details	Qiu et al. (2008)		ANB1 FUN12 GCD11 RPG1 SUI3 TIF11 TIF3 TIF4631 TIF4632 TIF5

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	TID3	No Comments	Cheeseman IM, et al. (2002)
View Run	TOR2	No Comments	Reinke A, et al. (2004)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#10 Mitotic Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	TIF3	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..92]	PSI-BLAST	46.522879	Nuclear ribonucleoprotein A1 (RNP A1, UP1)
2	View Details	[93..217]	PSI-BLAST	14.045757	Poly(A)-binding protein
3	View Details	[218..336]	PSI-BLAST	14.045757	Poly(A)-binding protein
4	View Details	[337..436]	deduced	N/A	No confident structure predictions are available.

Functions predicted (by domain):

#

Gene Ontology predictions

1

Term	Confidence	Notes
RNA binding	4.50438526063124	bayes_pls_golite062009
structural molecule activity	4.28696884073791	bayes_pls_golite062009
translation initiation factor activity	3.27900316559423	bayes_pls_golite062009
nucleic acid binding	3.2381342715237	bayes_pls_golite062009
mRNA binding	2.92795844574986	bayes_pls_golite062009
binding	2.62183511329394	bayes_pls_golite062009
	1.49431666307335	bayes_pls_golite062009
single-stranded RNA binding	0.90573796517364	bayes_pls_golite062009
RNA cap binding	0.65495719965273	bayes_pls_golite062009
protein binding	0.42571879933058	bayes_pls_golite062009
translation regulator activity	0.38120065819101	bayes_pls_golite062009
poly-pyrimidine tract binding	0.32812362201346	bayes_pls_golite062009
DNA binding	0.139020718026377	bayes_pls_golite062009
translation factor activity, nucleic acid binding	0.128616879457381	bayes_pls_golite062009
transcription regulator activity	0.0995666109361987	bayes_pls_golite062009

2

Term	Confidence	Notes
RNA binding	4.50438526063124	bayes_pls_golite062009
structural molecule activity	4.28696884073791	bayes_pls_golite062009
translation initiation factor activity	3.27900316559423	bayes_pls_golite062009
nucleic acid binding	3.2381342715237	bayes_pls_golite062009
mRNA binding	2.92795844574986	bayes_pls_golite062009
binding	2.62183511329394	bayes_pls_golite062009
	1.49431666307335	bayes_pls_golite062009
single-stranded RNA binding	0.90573796517364	bayes_pls_golite062009
RNA cap binding	0.65495719965273	bayes_pls_golite062009
protein binding	0.42571879933058	bayes_pls_golite062009
translation regulator activity	0.38120065819101	bayes_pls_golite062009
poly-pyrimidine tract binding	0.32812362201346	bayes_pls_golite062009
DNA binding	0.139020718026377	bayes_pls_golite062009
translation factor activity, nucleic acid binding	0.128616879457381	bayes_pls_golite062009
transcription regulator activity	0.0995666109361987	bayes_pls_golite062009

3

No functions predicted.

4

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

YRC Informatics Platform - Version 3.0
Created and Maintained by: Michael Riffle