Protein Overview: HRR25

General Information:
Name(s) found:	HRR25 / YPL204W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6325052\|ref\|NP_015120.1\| Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta); Hrr25p [Saccharomy [NCBI NR] gi\|234768\|gb\|AAB19685.1\| HRR25=putative protein kinase [Saccharomyces cerevisiae, Peptide, 494 aa] [NCBI NR] gi\|171706\|gb\|AAA34687.1\| protein kinase [Saccharomyces cerevisiae] [NCBI NR] gi\|1370424\|emb\|CAA97918.1\| HRR25 [Saccharomyces cerevisiae] [NCBI NR] gi\|123515\|sp\|P29295.1\|HRR25_YEAST RecName: Full=Casein kinase I homolog HRR25 [NCBI NR] pir\|\|A40860 probable protein kinase HRR25 (EC 2.7.1.-) - yeast (Saccharomyces cerevisiae) [NCBI NR] Casein kinase I homolog HRR25 OS=Saccharomyces cerevisiae GN=HRR25 PE=1 SV=1 [Swiss-Prot] (P29295) Casein kinase I homolog HRR25 (EC 2.7.1.-) [Swiss-Prot] HRR25 Casein kinase I, ser/thr/tyr protein kinase [mips] Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta) [SGD] Chr XVI from 164275-165759 [hmr_yeast5_DM3-1_pombe.fasta] HRR25, Chr XVI from 164275-165759 [yeast_orfs2] HRR25 SGDID:S0006125, Chr XVI from 164275-165759, Verified ORF [yeastflysequest.fasta] Hrr25p [Saccharomyces cerevisiae]gi\|1370424\|emb\|CAA97918.1\| HRR25 [Saccharomyces cerevisiae]gi\|123515\|sp\|P29295\|HR25_YEAST Casein kinase I homolog HRR25gi\|101506\|pir\|\|A40860 probable protein kinase HRR25 (EC 2.7.1.-) - yeast (Saccharomyces cerevisiae) [nrdb_11152004_Chlamydomonas] HRR25 SGDID:S000006125, Chr XVI from 164275-165759, Verified ORF, "Protein kinase involved in regulating diverse events including vesicular trafficking, gene expression, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mamma [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] HRR25 SGDID:S000006125, Chr XVI from 164275-165759, Verified ORF, "Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinas [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] HRR25=putative protein kinase [Saccharomyces cerevisiae, Peptide, 494 aa] [nr-271106-contam.fasta] Protein kinase involved in regulating diverse events including vesicular trafficking, gene expression, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta); Hrr25p [Saccharomyces cerevis [nr-271106-contam.fasta] protein kinase [nr-271106-contam.fasta] Casein kinase I homolog HRR25 [nr-271106-contam.fasta] HRR25 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] HRR25 SGDID:S000006125, Chr XVI from 164275-165759, Verified ORF, "Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta)" [yeast-bovrabprots.fasta] HRR25 SGDID:S000006125, Chr XVI from 164275-165759, Verified ORF, "Protein kinase involved in regulating diverse events including vesicular trafficking, gene expression, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta)" [yeast-contam_custom.fasta] [chemostatdb.fasta] Casein kinase I homolog HRR25 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=HRR25 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] HRR25 SGDID:S000006125, Chr XVI from 164276-165760, Genome Release 64-1-1, Verified ORF, "Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta)" [yeast-030211-contam.fasta] HRR25 SGDID:S000006125, Chr XVI from 164276-165760, Genome Release 64-2-1, Verified ORF, "Protein kinase; involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; phosphorylates the COPII coat; phosphorylates Tif6p which plays a critical role in 60S ribosomal subunit biogenesis; interacts with Sit4p phosphatase; homolog of mammalian casein kinase 1delta (CK1delta)" [20141208_orf_trans.fasta] YPL204W SGDID:S000006125, Chr XVI from 164276-165760, Genome Release 64-1-1, Verified ORF, "Protein kinase involved in regulating diverse events including vesicular trafficking, DNA repair, and chromosome segregation; binds the CTD of RNA pol II; homolog of mammalian casein kinase 1delta (CK1delta)" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	494 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] plasma membrane [IDA]
Biological Process:	DNA repair [IMP ] nuclear division [TAS] ribosomal small subunit biogenesis [IMP] chromosome segregation [TAS] mitosis [IMP] meiosis [IMP]
Molecular Function:	casein kinase activity [IDA][ISS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MDLRVGRKFR IGRKIGSGSF GDIYHGTNLI SGEEVAIKLE SIRSRHPQLD YESRVYRYLS  60
   61 GGVGIPFIRW FGREGEYNAM VIDLLGPSLE DLFNYCHRRF SFKTVIMLAL QMFCRIQYIH 120
  121 GRSFIHRDIK PDNFLMGVGR RGSTVHVIDF GLSKKYRDFN THRHIPYREN KSLTGTARYA 180
  181 SVNTHLGIEQ SRRDDLESLG YVLIYFCKGS LPWQGLKATT KKQKYDRIME KKLNVSVETL 240
  241 CSGLPLEFQE YMAYCKNLKF DEKPDYLFLA RLFKDLSIKL EYHNDHLFDW TMLRYTKAMV 300
  301 EKQRDLLIEK GDLNANSNAA SASNSTDNKS ETFNKIKLLA MKKFPTHFHY YKNEDKHNPS 360
  361 PEEIKQQTIL NNNAASSLPE ELLNALDKGM ENLRQQQPQQ QVQSSQPQPQ PQQLQQQPNG 420
  421 QRPNYYPEPL LQQQQRDSQE QQQQVPMATT RATQYPPQIN SNNFNTNQAS VPPQMRSNPQ 480
  481 QPPQDKPAGQ SIWL

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		DIM1 ENP1 HRR25 LTV1 RIO2 TSR1
View Details	(MIPS) Mewes HW, et al. (2004)		HRR25 YCK1 YCK2 YCK3
View Details	Gavin AC, et al. (2006)		HRR25 LTV1 NOB1 RIO2 TSR1
View Details	Gavin AC, et al. (2002)		ADH1 ARP9 ATG26 BEM2 BUD3 CBF5 COP1 CYR1 DIM1 ECM16 EMG1 ENP1 HRR25 IMG1 IMG2 IML1 IMP3 IMP4 KRE33 KRR1 LTV1 MHR1 MPP10 MRH4 MRM1 MRP20 MRP49 MRP7 MRPL1 MRPL10 MRPL13 MRPL16 MRPL17 MRPL19 MRPL20 MRPL23 MRPL24 MRPL25 MRPL27 MRPL28 MRPL3 MRPL35 MRPL36 MRPL39 MRPL4 MRPL44 MRPL51 MRPL6 MRPL7 MRPL8 NOB1 NOC4 NOP1 NOP14 NUG1 PAA1 PFK1 PNO1 PRP43 PTC3 RAD50 RCL1 RET2 RIO2 ROM2 RRP12 RTC6 SAM1 SEC1 SEC26 SEC27 SIN4 SLX9 SNF12 SNF2 SNF5 SNF6 SOF1 SWI1 SWI3 SWP82 SYP1 TAF5 TSR1 UTP15 UTP20 UTP22 UTP6 UTP7 VPS1 VPS15 YDR115W YGR054W YML6
View Details	Ho Y, et al. (2002)		ABP1 ACO1 AIM41 ARP4 ATE1 ATP4 BUD14 CAR2 CDC25 CDC33 CKA2 COR1 CRZ1 CYS4 DCP1 DCP2 DNM1 EAF1 EDC3 EDE1 EGD2 ENP1 EPL1 ESA1 FPR3 GAS1 GCN3 GLC7 GPH1 HHF1, HHF2 HRR25 HSP104 HXT7 HYP2 IPP1 LOC1 LSP1 LTV1 MDH1 MDS3 MGE1 NSA1 NUG1 OYE2 PEX19 PIL1 PIN4 POR1 PTC4 PUF3 RIO2 RPC19 RPM2 SAP185 SAP190 SAS10 SEC2 SEC23 SES1 SFB3 SGM1 SIT4 TAF14 TAF4 TGL1 TIP41 TMA19 TRA1 TSR1 TUF1 VMA4 YBR225W YER138C YRA1
View Details	Qiu et al. (2008)		HRR25 YCK1 YCK2

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	RAD51	HRR25	1	Unpublished Fields Lab Data
View Screen	UBC7	HRR25	1	Unpublished Fields Lab Data
View Screen	YPR097W	HRR25	1	Unpublished Fields Lab Data
View Screen	CDC48	HRR25	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	HRR25	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..52] [68..84]	PSI-BLAST	1370.0	Casein kinase-1, CK1
2	View Details	[53..67] [85..347]	PSI-BLAST	1370.0	Casein kinase-1, CK1
3	View Details	[348..494]	deduced	N/A	No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
phosphotransferase activity, alcohol group as acceptor	5.49988294224815	bayes_pls_golite062009
protein kinase activity	5.4586557943719	bayes_pls_golite062009
kinase activity	5.3287080791198	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	4.96052051118534	bayes_pls_golite062009
protein serine/threonine kinase activity	4.46381887831481	bayes_pls_golite062009
transferase activity	3.97138602499442	bayes_pls_golite062009
protein tyrosine kinase activity	2.8153155526437	bayes_pls_golite062009
binding	2.74863538127007	bayes_pls_golite062009
transmembrane receptor activity	2.59526003830791	bayes_pls_golite062009
transmembrane receptor protein kinase activity	2.54265774272269	bayes_pls_golite062009
signal transducer activity	2.40586892801446	bayes_pls_golite062009
molecular transducer activity	2.40586892801446	bayes_pls_golite062009
receptor activity	2.39311927684508	bayes_pls_golite062009
tubulin binding	2.18641675163967	bayes_pls_golite062009
transmembrane receptor protein tyrosine kinase activity	2.16831387024372	bayes_pls_golite062009
cytoskeletal protein binding	2.12615078788254	bayes_pls_golite062009
receptor signaling protein activity	2.10379226653669	bayes_pls_golite062009
ATP binding	1.97600611746091	bayes_pls_golite062009
adenyl ribonucleotide binding	1.93596671856664	bayes_pls_golite062009
adenyl nucleotide binding	1.90928021591891	bayes_pls_golite062009
microtubule binding	1.88110801192607	bayes_pls_golite062009
receptor signaling protein serine/threonine kinase activity	1.81249386965144	bayes_pls_golite062009
purine ribonucleotide binding	1.65732943334212	bayes_pls_golite062009
ribonucleotide binding	1.65726906484847	bayes_pls_golite062009
purine nucleotide binding	1.63763394711025	bayes_pls_golite062009
nucleotide binding	1.63195828048121	bayes_pls_golite062009
actin binding	1.33479685165255	bayes_pls_golite062009
protein binding	1.22565748038754	bayes_pls_golite062009
catalytic activity	1.21655087018101	bayes_pls_golite062009
transporter activity	1.11833481501707	bayes_pls_golite062009
transmembrane transporter activity	0.97575992765505	bayes_pls_golite062009
growth factor binding	0.86076618871384	bayes_pls_golite062009
ligase activity, forming aminoacyl-tRNA and related compounds	0.835012681606991	bayes_pls_golite062009
ligase activity, forming carbon-oxygen bonds	0.835012681606991	bayes_pls_golite062009
aminoacyl-tRNA ligase activity	0.832111648073981	bayes_pls_golite062009
nucleic acid binding	0.794058505655489	bayes_pls_golite062009
transcription regulator activity	0.775404673285876	bayes_pls_golite062009
DNA binding	0.714163549867672	bayes_pls_golite062009
ligase activity	0.665445021164512	bayes_pls_golite062009
structural constituent of cytoskeleton	0.612883428421652	bayes_pls_golite062009
transcription factor activity	0.563262875317016	bayes_pls_golite062009
cyclin-dependent protein kinase activity	0.499492353295369	bayes_pls_golite062009
protein serine/threonine/tyrosine kinase activity	0.48345772948903	bayes_pls_golite062009
substrate-specific transporter activity	0.451090007290896	bayes_pls_golite062009
magnesium ion binding	0.393328812780268	bayes_pls_golite062009
MAP kinase kinase kinase activity	0.37115026509016	bayes_pls_golite062009
telethonin binding	0.366817170817532	bayes_pls_golite062009
MAP kinase kinase activity	0.35402045013148	bayes_pls_golite062009
kinase binding	0.26752427107238	bayes_pls_golite062009
MAP kinase activity	0.26024936404169	bayes_pls_golite062009
actin filament binding	0.2403700250846	bayes_pls_golite062009
protein complex binding	0.239339759110451	bayes_pls_golite062009
transmembrane receptor protein serine/threonine kinase activity	0.20643152368029	bayes_pls_golite062009
transforming growth factor beta receptor activity	0.20643152368029	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	0.205652638854091	bayes_pls_golite062009
RNA binding	0.202560498527088	bayes_pls_golite062009
receptor binding	0.197402253061255	bayes_pls_golite062009
protein kinase C activity	0.0795689006739899	bayes_pls_golite062009
protein kinase binding	0.00674998152095019	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.0040394359628233	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.90

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2