Protein Overview: POL2

General Information:
Name(s) found:	POL2 / YNL262W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6324067\|ref\|NP_014137.1\| Catalytic subunit of DNA polymerase (II) epsilon, one of the major chromosomal DNA replication polymerases characterized by processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair; P [NCBI NR] gi\|171409\|gb\|AAA88711.1\| DNA polymerase II [Saccharomyces cerevisiae] [NCBI NR] gi\|1302317\|emb\|CAA96169.1\| POL2 [Saccharomyces cerevisiae] [NCBI NR] gi\|118841\|sp\|P21951.1\|DPOE_YEAST RecName: Full=DNA polymerase epsilon catalytic subunit A; Short=DNA polymerase II subunit A [NCBI NR] pir\|\|A36028 DNA-directed DNA polymerase (EC 2.7.7.7) II catalytic chain - yeast (Saccharomyces cerevisiae) [NCBI NR] DNA polymerase epsilon catalytic subunit A OS=Saccharomyces cerevisiae GN=POL2 PE=1 SV=1 [Swiss-Prot] POL2 DNA-directed DNA polymerase epsilon, catalytic subunit A [mips] Catalytic subunit of DNA polymerase (II) epsilon, a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p [SGD] Chr XIV from 148211-154879 [yeastorf3.fasta] (P21951) DNA polymerase epsilon, catalytic subunit A (EC 2.7.7.7) (DNA polymerase II subunit A) [4932.FASTACCERV.txt] POL2, Chr XIV from 148211-154879 [yeast_orfs2] POL2 SGDID:S0005206, Chr XIV from 148211-154879, Verified ORF [yeastflysequest.fasta] Catalytic subunit of DNA polymerase epsilon, one of the major chromosomal DNA replication polymerases characterized by processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair [Saccharomyces cerevisiae]gi\|13023 [nrdb_11152004_Chlamydomonas] POL2 SGDID:S000005206, Chr XIV from 148213-154881, Verified ORF, "Catalytic subunit of DNA polymerase epsilon, one of the major chromosomal DNA replication polymerases characterized by processivity and proofreading exonuclease activity; also involved in D [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] POL2 SGDID:S000005206, Chr XIV from 148213-154881, Verified ORF, "Catalytic subunit of DNA polymerase (II) epsilon, one of the major chromosomal DNA replication polymerases characterized by processivity and proofreading exonuclease activity; also involved [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] POL2 SGDID:S000005206, Chr XIV from 148213-154881, Verified ORF, "Catalytic subunit of DNA polymerase (II) epsilon, a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; also involved in DNA synthesis d [yeast-200209-contam.fasta] POL2 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] DNA polymerase II [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Catalytic subunit of DNA polymerase epsilon, one of the major chromosomal DNA replication polymerases characterized by processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair; Pol2p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] DNA polymerase epsilon, catalytic subunit A (DNA polymerase II subunit A) [nr-271106-contam.fasta] POL2 SGDID:S000005206, Chr XIV from 148213-154881, Verified ORF, "Catalytic subunit of DNA polymerase (II) epsilon, a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p" [yeast-bovrabprots.fasta] POL2 SGDID:S000005206, Chr XIV from 148213-154881, Verified ORF, "Catalytic subunit of DNA polymerase epsilon, one of the major chromosomal DNA replication polymerases characterized by processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair" [yeast-contam_custom.fasta] [chemostatdb.fasta] DNA polymerase epsilon catalytic subunit A OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=POL2 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] POL2 SGDID:S000005206, Chr XIV from 148212-154880, Genome Release 64-1-1, Verified ORF, "Catalytic subunit of DNA polymerase (II) epsilon, a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p" [yeast-030211-contam.fasta] POL2 SGDID:S000005206, Chr XIV from 148212-154880, Genome Release 64-2-1, Verified ORF, "Catalytic subunit of DNA polymerase (II) epsilon; a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; participates in leading-strand synthesis during DNA replication; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p" [20141208_orf_trans.fasta] YNL262W SGDID:S000005206, Chr XIV from 148212-154880, Genome Release 64-1-1, Verified ORF, "Catalytic subunit of DNA polymerase (II) epsilon, a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	2222 amino acids
Gene Ontology:
Cellular Component:	replication fork [IDA] epsilon DNA polymerase complex [IDA]
Biological Process:	lagging strand elongation [TAS] leading strand elongation [TAS] nucleotide-excision repair [TAS] mismatch repair [TAS] DNA synthesis involved in DNA repair [IMP] chromatin silencing at telomere [IMP]
Molecular Function:	nucleotide binding [IEA] nucleic acid binding [IEA] DNA binding [IEA] zinc ion binding [IEA] DNA-directed DNA polymerase activity [IEA][IMP] nucleotidyltransferase activity [IEA] metal ion binding [IEA] double-stranded DNA binding [IDA] transferase activity [IEA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MMFGKKKNNG GSSTARYSAG NKYNTLSNNY ALSAQQLLNA SKIDDIDSMM GFERYVPPQY  60
   61 NGRFDAKDID QIPGRVGWLT NMHATLVSQE TLSSGSNGGG NSNDGERVTT NQGISGVDFY 120
  121 FLDEEGGSFK STVVYDPYFF IACNDESRVN DVEELVKKYL ESCLKSLQII RKEDLTMDNH 180
  181 LLGLQKTLIK LSFVNSNQLF EARKLLRPIL QDNANNNVQR NIYNVAANGS EKVDAKHLIE 240
  241 DIREYDVPYH VRVSIDKDIR VGKWYKVTQQ GFIEDTRKIA FADPVVMAFD IETTKPPLKF 300
  301 PDSAVDQIMM ISYMIDGEGF LITNREIISE DIEDFEYTPK PEYPGFFTIF NENDEVALLQ 360
  361 RFFEHIRDVR PTVISTFNGD FFDWPFIHNR SKIHGLDMFD EIGFAPDAEG EYKSSYCSHM 420
  421 DCFRWVKRDS YLPQGSQGLK AVTQSKLGYN PIELDPELMT PYAFEKPQHL SEYSVSDAVA 480
  481 TYYLYMKYVH PFIFSLCTII PLNPDETLRK GTGTLCEMLL MVQAYQHNIL LPNKHTDPIE 540
  541 RFYDGHLLES ETYVGGHVES LEAGVFRSDL KNEFKIDPSA IDELLQELPE ALKFSVEVEN 600
  601 KSSVDKVTNF EEIKNQITQK LLELKENNIR NELPLIYHVD VASMYPNIMT TNRLQPDSIK 660
  661 AERDCASCDF NRPGKTCARK LKWAWRGEFF PSKMDEYNMI KRALQNETFP NKNKFSKKKV 720
  721 LTFDELSYAD QVIHIKKRLT EYSRKVYHRV KVSEIVEREA IVCQRENPFY VDTVKSFRDR 780
  781 RYEFKGLAKT WKGNLSKIDP SDKHARDEAK KMIVLYDSLQ LAHKVILNSF YGYVMRKGSR 840
  841 WYSMEMAGIT CLTGATIIQM ARALVERVGR PLELDTDGIW CILPKSFPET YFFTLENGKK 900
  901 LYLSYPCSML NYRVHQKFTN HQYQELKDPL NYIYETHSEN TIFFEVDGPY KAMILPSSKE 960
  961 EGKGIKKRYA VFNEDGSLAE LKGFELKRRG ELQLIKNFQS DIFKVFLEGD TLEGCYSAVA1020
 1021 SVCNRWLDVL DSHGLMLEDE DLVSLICENR SMSKTLKEYE GQKSTSITTA RRLGDFLGED1080
 1081 MVKDKGLQCK YIISSKPFNA PVTERAIPVA IFSADIPIKR SFLRRWTLDP SLEDLDIRTI1140
 1141 IDWGYYRERL GSAIQKIITI PAALQGVSNP VPRVEHPDWL KRKIATKEDK FKQTSLTKFF1200
 1201 SKTKNVPTMG KIKDIEDLFE PTVEEDNAKI KIARTTKKKA VSKRKRNQLT NEEDPLVLPS1260
 1261 EIPSMDEDYV GWLNYQKIKW KIQARDRKRR DQLFGNTNSS RERSALGSMI RKQAESYANS1320
 1321 TWEVLQYKDS GEPGVLEVFV TINGKVQNIT FHIPKTIYMK FKSQTMPLQK IKNCLIEKSS1380
 1381 ASLPNNPKTS NPAGGQLFKI TLPESVFLEE KENCTSIFND ENVLGVFEGT ITPHQRAIMD1440
 1441 LGASVTFRSK AMGALGKGIQ QGFEMKDLSM AENERYLSGF SMDIGYLLHF PTSIGYEFFS1500
 1501 LFKSWGDTIT ILVLKPSNQA QEINASSLGQ IYKQMFEKKK GKIETYSYLV DIKEDINFEF1560
 1561 VYFTDISKLY RRLSQETTKL KEERGLQFLL LLQSPFITKL LGTIRLLNQM PIVKLSLNEV1620
 1621 LLPQLNWQPT LLKKLVNHVL SSGSWISHLI KLSQYSNIPI CNLRLDSMDY IIDVLYARKL1680
 1681 KKENIVLWWN EKAPLPDHGG IQNDFDLNTS WIMNDSEFPK INNSGVYDNV VLDVGVDNLT1740
 1741 VNTILTSALI NDAEGSDLVN NNMGIDDKDA VINSPSEFVH DAFSNDALNV LRGMLKEWWD1800
 1801 EALKENSTAD LLVNSLASWV QNPNAKLFDG LLRYHVHNLT KKALLQLVNE FSALGSTIVY1860
 1861 ADRNQILIKT NKYSPENCYA YSQYMMKAVR TNPMFSYLDL NIKRYWDLLI WMDKFNFSGL1920
 1921 ACIEIEEKEN QDYTAVSQWQ LKKFLSPIYQ PEFEDWMMII LDSMLKTKQS YLKLNSGTQR1980
 1981 PTQIVNVKKQ DKEDSVENSL NGFSHLFSKP LMKRVKKLFK NQQEFILDPQ YEADYVIPVL2040
 2041 PGSHLNVKNP LLELVKSLCH VMLLSKSTIL EIRTLRKELL KIFELREFAK VAEFKDPSLS2100
 2101 LVVPDFLCEY CFFISDIDFC KAAPESIFSC VRCHKAFNQV LLQEHLIQKL RSDIESYLIQ2160
 2161 DLRCSRCHKV KRDYMSAHCP CAGAWEGTLP RESIVQKLNV FKQVAKYYGF DILLSCIADL2220
 2221 TI

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		DPB2 DPB3 DPB4 LHP1 POL2 STE50 TIF4631 TIF4632
View Details	(MIPS) Mewes HW, et al. (2004)		CDC45 DPB11 DPB2 DPB3 MCM2 MCM3 MCM4 MCM5 MCM6 MCM7 ORC1 ORC2 ORC3 ORC4 ORC5 ORC6 POL2 POL3 POL31 POL32
View Details	Krogan NJ, et al. (2006)		DPB2 DPB3 DPB4 INM1 MBA1 MCM2 POL2 RPN4
View Details	Gavin AC, et al. (2006)		DPB2 DPB4 POL2
View Details	Gavin AC, et al. (2002)		AAR2 ASC1 BRX1 CBF5 CDC33 CIC1 CTF18 CTF8 DBP2 DBP9 DPB2 DPB4 DRS1 ELG1 ERB1 FUN12 GAR1 HAS1 HCA4 HEK2 HHF1, HHF2 IML1 ISW1 ISW2 KAP114 KRE33 KRR1 LHP1 MAK21 MDN1 MIS1 MOT1 MRP7 NAB3 NAB6 NAM7 NAP1 NHP2 NIP1 NOC2 NOG1 NOP1 NOP12 NOP2 NOP4 NOP56 NOP6 NOP7 NPL3 NPL6 NRD1 NRP1 NUG1 OLA1 POL2 PRP4 PUB1 PUF6 PUS7 RBG1 RET1 RFC1 RFC2 RFC3 RFC4 RFC5 RLP7 ROM2 RPA135 RPC82 RPO31 RRP12 SBP1 SGN1 SMB1 SMD3 SNF2 SNP1 SNU114 SNU66 SRO9 STH1 STM1 STO1 SUI3 SWI1 TIF1, TIF2 TIF4631 TIF4632 TOP1 TOP2 URB1 UTP10 UTP20 UTP21 VPS1 VPS13 YMR181C YMR310C YTM1
View Details	Ho Y, et al. (2002)		CIC1 CKA1 CKA2 CKB1 CKB2 DBP10 ENP2 GDE1 HHF1, HHF2 HOT1 KRI1 MRPL1 NOC3 NOP12 NOP2 NOP7 PDI1 POB3 POL2 PUF6 PWP1 RRP14 RRP5 SFP1 SPT16 SSF1 TIF4631 TRL1 UTP22 VMR1 VPS73 WTM2 YRA1
View Details	Qiu et al. (2008)		CDC45 DPB11 DPB2 DPB3 DPB4 HCS1 MCM2 MCM3 MCM4 MCM5 MCM6 MCM7 ORC1 ORC2 ORC3 ORC4 ORC5 ORC6 POL1 POL12 POL2 POL3 POL30 POL31 POL32 PRI1 PRI2

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	POL2	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..100]	deduced	N/A	No confident structure predictions are available.
2	View Details	[101..136] [246..534]	PSI-BLAST	274.0	Exonuclease domain of family B (archaeal and phage) DNA polymerases; T4-like DNA polymerase
3	View Details	[137..245] [535..555] [575..678]	PSI-BLAST	274.0	Exonuclease domain of family B (archaeal and phage) DNA polymerases; T4-like DNA polymerase
4	View Details	[556..574] [679..857]	PSI-BLAST	274.0	Exonuclease domain of family B (archaeal and phage) DNA polymerases; T4-like DNA polymerase
5	View Details	[858..1098]	PSI-BLAST	13.0	No description for 1b1fR_ was found.
6	View Details	[1099..1232]	MSA	2.037994	View MSA. No confident structure predictions are available.
7	View Details	[1233..1924]	deduced	N/A	No confident structure predictions are available.
8	View Details	[1925..2222]	MSA	3.018912	View MSA. No confident structure predictions are available.

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Protein Overview: POL2

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: