Protein Overview: CMD1

General Information:
Name(s) found:	CMD1 / YBR109C [SGD]
Description(s) found: Found 38 descriptions. SHOW ALL	Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca++ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin [SGD]
Organism:	Saccharomyces cerevisiae
Length:	147 amino acids
Gene Ontology:
Cellular Component:	incipient cellular bud site [IDA] cellular bud tip [IDA] central plaque of spindle pole body [IDA] cytoplasm [TAS] cellular bud neck [IDA] mating projection tip [IDA]
Biological Process:	cytoskeleton organization [IMP] cell budding [IMP] vacuole fusion, non-autophagic [IMP ] mitosis [TAS] endocytosis [IMP]
Molecular Function:	protein binding [TAS] calcium ion binding [IDA] calcium-dependent protein binding [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSSNLTEEQI AEFKEAFALF DKDNNGSISS SELATVMRSL GLSPSEAEVN DLMNEIDVDG  60
   61 NHQIEFSEFL ALMSRQLKSN DSEQELLEAF KVFDKNGDGL ISAAELKHVL TSIGEKLTDA 120
  121 EVDDMLREVS DGSGEINIQQ FAALLSK

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ABP1 BSP1 CMD1 CMP2 CRN1 INP52 LSP1 MLC1 MYO2 MYO3 MYO4 MYO5 NOP53 PIL1 RPL40B, RPL40A RVS161 SAC6 SHE3 YGL242C YNL208W
View Details	(MIPS) Mewes HW, et al. (2004)		BBP1 CDC31 CMD1 CNM67 KAR1 NUD1 SPC105 SPC110 SPC24 SPC25 SPC29 SPC42 SPC72
View Details	Krogan NJ, et al. (2006)		CMD1 CMK1 CMK2 CMP2 CNA1 CNB1 MYO3 MYO5 YGL242C
View Details	Gavin AC, et al. (2002)		CMD1 CSL4 CTR9 DIS3 ECM16 GCN1 MAM33 MLC1 MTR3 MYO2 NMD5 RRP4 RRP40 RRP42 RRP43 RRP45 RRP46 RRP6 SAM1 SHE4 SKI6 SKI7 SRP1 UTP10
View Details	Ho Y, et al. (2002)		CMD1 CMK1 CMK2 CMP2 CNA1 COF1 CPR1 EDE1 HCH1 HUL5 HYP2 IDH1 ILS1 IPP1 MLC1 MYO2 MYO3 MYO4 MYO5 PGM2 PST2 RFC3 RPN7 SAY1 SHE3 SHE4 SOD1 SPC110 TEF4 UBA1 VAS1 VPH2 VPS13 YNK1
View Details	Qiu et al. (2008)		CDC31 CMD1 HSK3 KAR1 SPC110 SPC29 SPC42 SPC72

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	TOR1	No Comments	Reinke A, et al. (2004)
View Run	TOR2	No Comments	Reinke A, et al. (2004)
View Run	LST8	No Comments	Reinke A, et al. (2004)
View Run	TOR2	No Comments	Reinke A, et al. (2004)
View Run	KOG1	No Comments	Reinke A, et al. (2004)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	BOI1	Sample bob1 (2nd set) from october 2005	McCusker D, et al (2007)
View Run	MLP2	#33 Asynchronous SPB prep	Keck JM, et al. (2011)
View Run	MLP2	#19 Asynchronous Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#20 Asynchronous Prep1-TiO2 Phosphopeptide enrichment, PartB	Keck JM, et al. (2011)
View Run	MLP2	#25 Asynchronous Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2	Keck JM, et al. (2011)
View Run	MLP2	#27 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps3-5	Keck JM, et al. (2011)
View Run	MLP2	#21 Asynchronous Prep2-IMAC Phosphopeptide enrichment A1	Keck JM, et al. (2011)
View Run	MLP2	#22 Asynchronous Prep2-IMAC Phosphopeptide enrichment B1-2	Keck JM, et al. (2011)
View Run	MLP2	#34 Asynchronous SPB prep	Keck JM, et al. (2011)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#35 Asynchronous SPB prep	Keck JM, et al. (2011)
View Run	MLP2	#31 Asynchronous Prep (Protease cleavage)	Keck JM, et al. (2011)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#14 Mitotic Prep2-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#13 Mitotic Prep2-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#10 Mitotic Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#02 Alpha Factor Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#01 Alpha factor Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#28 Asynchronous Prep5-TiO2 Phosphopeptide Enrichment	Keck JM, et al. (2011)
View Run	MLP2	#06 Alpha Factor Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#08 Alpha Factor Prep4-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#15 Mitotic 2nd-TiO2 enriched, new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#19b Asynchronous Prep1-new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#05 Alpha Factor Prep2-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#07 Alpha Factor Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	None Entered	#17 Mitotic Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#19a Asynchronous Prep1-new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	CMD1	YML094C-A	2	Unpublished Fields Lab Data
View Screen	CMD1	YMR111C	2	Unpublished Fields Lab Data
View Screen	CMD1	YMR111C	2	Unpublished Fields Lab Data
View Screen	CMD1	MET2	2	Unpublished Fields Lab Data
View Screen	CMD1	YJR028W	1	Unpublished Fields Lab Data
View Screen	CMD1	VPS51	1	Unpublished Fields Lab Data
View Screen	CMD1	GAL2	1	Unpublished Fields Lab Data
View Screen	CMD1	YBR099C	1	Unpublished Fields Lab Data
View Screen	CMD1	EMP70	1	Unpublished Fields Lab Data
View Screen	CMD1	CPR4	1	Unpublished Fields Lab Data
View Screen	CMD1	SYM1	1	Unpublished Fields Lab Data
View Screen	CMD1	QRI7	1	Unpublished Fields Lab Data
View Screen	CMD1	BET5	1	Unpublished Fields Lab Data
View Screen	CMD1	MHR1	1	Unpublished Fields Lab Data
View Screen	CMD1	FMP48	1	Unpublished Fields Lab Data
View Screen	CMD1	SEC72	1	Unpublished Fields Lab Data
View Screen	CMD1	ESC1	1	Unpublished Fields Lab Data
View Screen	CMD1	SUI1	1	Unpublished Fields Lab Data
View Screen	CMD1	TRF5	1	Unpublished Fields Lab Data
View Screen	CMD1	SHE3	1	Unpublished Fields Lab Data
View Screen	CMD1	YNR025C	1	Unpublished Fields Lab Data
View Screen	CMD1	PPH3	1	Unpublished Fields Lab Data
View Screen	CMD1	VAM10	1	Unpublished Fields Lab Data
View Screen	CMD1	KGD2	1	Unpublished Fields Lab Data
View Screen	CMD1	BIM1	1	Unpublished Fields Lab Data
View Screen	CMD1	ARO8	1	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	CMD1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..77]	PSI-BLAST	481.67837	Calmodulin
2	View Details	[78..147]	PSI-BLAST	481.67837	Calmodulin

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
troponin T binding	7.68526659589559	bayes_pls_golite062009
phosphoprotein phosphatase activity	7.47426847861728	bayes_pls_golite062009
protein serine/threonine phosphatase activity	6.37104157544514	bayes_pls_golite062009
structural constituent of muscle	6.34041234021534	bayes_pls_golite062009
actin-dependent ATPase activity	6.31366142616096	bayes_pls_golite062009
phosphatase activity	6.28157965296365	bayes_pls_golite062009
phosphoric ester hydrolase activity	6.17799259119197	bayes_pls_golite062009
N-terminal myristoylation domain binding	5.746886320739	bayes_pls_golite062009
microfilament motor activity	5.62061944255026	bayes_pls_golite062009
calcium-dependent protein serine/threonine phosphatase activity	5.58369939851095	bayes_pls_golite062009
G-protein beta/gamma-subunit binding	5.51712867097471	bayes_pls_golite062009
myosin II heavy chain binding	5.49688740358168	bayes_pls_golite062009
hydrolase activity, acting on ester bonds	5.11186887221409	bayes_pls_golite062009
calcium-dependent protein serine/threonine phosphatase regulator activity	4.70715233640875	bayes_pls_golite062009
actin binding	3.96919556235483	bayes_pls_golite062009
calcium ion binding	3.79609619542776	bayes_pls_golite062009
myosin heavy chain binding	3.63014173976595	bayes_pls_golite062009
cytoskeletal protein binding	3.49668552859347	bayes_pls_golite062009
motor activity	3.25814691068414	bayes_pls_golite062009
binding	3.17556402087346	bayes_pls_golite062009
cation binding	2.62077305565026	bayes_pls_golite062009
metal ion binding	2.62077305565026	bayes_pls_golite062009
metal ion transmembrane transporter activity	2.61874575467092	bayes_pls_golite062009
ion binding	2.61312132078717	bayes_pls_golite062009
ion transmembrane transporter activity	2.57032504567157	bayes_pls_golite062009
cation transmembrane transporter activity	2.55650624542181	bayes_pls_golite062009
tubulin binding	2.51210014078446	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	2.37465721152046	bayes_pls_golite062009
actin filament binding	2.35534489389161	bayes_pls_golite062009
myosin binding	2.34125070430971	bayes_pls_golite062009
calcium sensitive guanylate cyclase activator activity	2.25146252368284	bayes_pls_golite062009
protein binding	2.21291432166051	bayes_pls_golite062009
microtubule binding	2.17136406283012	bayes_pls_golite062009
structural molecule activity	2.02132683356917	bayes_pls_golite062009
transcription regulator activity	1.82714164083189	bayes_pls_golite062009
signal transducer activity	1.56751504958972	bayes_pls_golite062009
molecular transducer activity	1.56751504958972	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	1.51057181410195	bayes_pls_golite062009
cation channel activity	1.49312394054212	bayes_pls_golite062009
kinase activity	1.48008348280256	bayes_pls_golite062009
nucleic acid binding	1.43263098461462	bayes_pls_golite062009
DNA binding	1.40714762620388	bayes_pls_golite062009
hydrolase activity	1.35435291164259	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	1.34982153616582	bayes_pls_golite062009
peptidase activity	1.33558871501581	bayes_pls_golite062009
clathrin binding	1.25585696981668	bayes_pls_golite062009
transporter activity	1.15928534291452	bayes_pls_golite062009
passive transmembrane transporter activity	1.05847414989139	bayes_pls_golite062009
channel activity	1.05847414989139	bayes_pls_golite062009
ion channel activity	1.0369591011376	bayes_pls_golite062009
substrate-specific channel activity	1.03529144716694	bayes_pls_golite062009
transferase activity	1.00143834284844	bayes_pls_golite062009
actin monomer binding	0.9954841049256	bayes_pls_golite062009
voltage-gated cation channel activity	0.986621437232479	bayes_pls_golite062009
nitric-oxide synthase binding	0.965098383344279	bayes_pls_golite062009
peptidase activity, acting on L-amino acid peptides	0.956941988596969	bayes_pls_golite062009
transmembrane transporter activity	0.945691505472547	bayes_pls_golite062009
voltage-gated ion channel activity	0.910148857310729	bayes_pls_golite062009
potassium channel activity	0.906643355854798	bayes_pls_golite062009
voltage-gated channel activity	0.896351900356978	bayes_pls_golite062009
phosphatase regulator activity	0.87149881541475	bayes_pls_golite062009
gated channel activity	0.855502499003241	bayes_pls_golite062009
cyclase regulator activity	0.84389905735679	bayes_pls_golite062009
guanylate cyclase regulator activity	0.84389905735679	bayes_pls_golite062009
protein phosphatase regulator activity	0.822713961141111	bayes_pls_golite062009
voltage-gated potassium channel activity	0.810245330492755	bayes_pls_golite062009
calcium channel regulator activity	0.7164851905066	bayes_pls_golite062009
substrate-specific transporter activity	0.693724160233023	bayes_pls_golite062009
peptide binding	0.3862375622127	bayes_pls_golite062009
	0.34446792626966	bayes_pls_golite062009
channel regulator activity	0.32809521759917	bayes_pls_golite062009
myosin II binding	0.311147527050891	bayes_pls_golite062009
active transmembrane transporter activity	0.246383459363256	bayes_pls_golite062009
ER retention sequence binding	0.234166588492671	bayes_pls_golite062009
structural constituent of cytoskeleton	0.116648335890738	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2