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Bait Protein: MLP2
Organism: Saccharomyces cerevisiae
Publication: Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561.
Comments: #05 Alpha Factor Prep2-TiO2 Flowthrough

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UNFILTERED Run Results: [filter]

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  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   YIL002W-A Putative protein of unknown function; identified by expression profiling and mass spectrometry 7 10 79.7% 0.0531 7729
[View Peptides]   MLP2 Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... 132 291 65.5% 0.0635 195140
[View Peptides]   RPL40B, RPL40A Fusion protein, identical to Rpl40Ap, that is cleaved to yield ubiquitin and a ribosomal protein of ... 5 98 47.7% 0.281 14554
[View Peptides]   RPS31 Fusion protein that is cleaved to yield a ribosomal protein of the small (40S) subunit and ubiquitin... 5 98 40.1% 0.236 17216
[View Peptides]   RPL10 Protein component of the large (60S) ribosomal subunit, responsible for joining the 40S and 60S subu... 11 18 39.8% 0.0299 25361
[View Peptides]   SPC110 Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... 23 42 32.0% 0.0163 111782
[View Peptides]   RPS1B Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Ap and has similarit... 5 10 28.6% 0.0144 28812
[View Peptides]   RPS1A Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Bp and has similarit... 5 10 27.5% 0.0144 28743
[View Peptides]   SSB1 Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... 12 23 25.3% 0.0138 66602
[View Peptides]   SSB2 Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... 12 23 25.3% 0.0138 66595
[View Peptides]   PDC1 Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates... 10 14 24.9% 0.00911 61495
[View Peptides]   SSA1 ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport;... 11 22 24.8% 0.0126 69768
[View Peptides]   SPC42 Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... 6 12 21.8% 0.0121 42271
[View Peptides]   ENO2 Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... 6 13 19.7% 0.0109 46914
[View Peptides]   EFT2, EFT1 Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein s... 10 28 17.3% 0.0122 93289
[View Peptides]   UBI4 Ubiquitin, becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-... 5 98 16.0% 0.0943 42826
[View Peptides]   PMA1 Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... 9 13 15.9% 0.00519 99619
[View Peptides]   NBP1 Spindle pole body (SPB) component, required for the insertion of the duplication plaque into the nuc... 3 3 15.0% 0.00345 37354
[View Peptides]   ENO1 Enolase I, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosph... 3 4 12.4% 0.00335 46816
[View Peptides]   YTM1 Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required fo... 2 3 10.7% 0.00239 51359
[View Peptides]   RPL13B Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl13Ap; not essential f... 2 4 10.6% 0.00737 22525
[View Peptides]   CNM67 Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... 6 9 10.5% 0.00568 67400
[View Peptides]   Reverse_YLR167W RPS31 SGDID:S000004157, Chr XII from 498949-499407, Verified ORF, "Fusion protein that is cleaved to... 2 5 10.5% 0.012 17216
[View Peptides]   CDC31 Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication i... 1 1 9.9% 0.00228 18751
[View Peptides]   SPC29 Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... 2 8 9.5% 0.0116 29280
[View Peptides]   Reverse_YNL225C CNM67 SGDID:S000005169, Chr XIV from 224470-222725, reverse complement, Verified ORF, "Component of ... 2 2 8.4% 0.00126 67400
[View Peptides]   Reverse_YMR186W HSC82 SGDID:S000004798, Chr XIII from 632354-634471, Verified ORF, "Cytoplasmic chaperone of the Hsp... 2 2 8.1% 0.00104 80900
[View Peptides]   Reverse_YLR075W RPL10 SGDID:S000004065, Chr XII from 282928-283593, Verified ORF, "Protein component of the large (6... 2 2 8.1% 0.0033 25361
[View Peptides]   RPS18B, RPS18A Protein component of the small (40S) ribosomal subunit; nearly identical to Rps18Ap and has similari... 1 1 7.5% 0.00251 17038
[View Peptides]   CMD1 Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... 1 3 6.1% 0.00748 16135
[View Peptides]   Reverse_YJR053W BFA1 SGDID:S000003814, Chr X from 533941-535665, Verified ORF, "Component of the GTPase-activating B... 1 1 5.7% 6.37E-4 66087
[View Peptides]   MPP10 Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts... 3 3 5.6% 0.00185 66953
[View Peptides]   Reverse_YLR457C NBP1 SGDID:S000004449, Chr XII from 1056768-1055809, reverse complement, Verified ORF, "Component of... 1 1 5.6% 0.00115 37354
[View Peptides]   BFA1 Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pa... 5 7 5.4% 0.00447 66087
[View Peptides]   HSC82 Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, a... 3 3 5.4% 0.00156 80900
[View Peptides]   Reverse_YNL014W HEF3 SGDID:S000004959, Chr XIV from 606320-609454, Verified ORF, "Translational elongation factor EF... 4 4 4.6% 0.0014 115868
[View Peptides]   Reverse_YDR385W,... EFT2 SGDID:S000002793, Chr IV from 1243220-1245748, Verified ORF, "Elongation factor 2 (EF-2), also ... 2 3 4.6% 0.0013 93289
[View Peptides]   MLP1 Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... 5 7 4.2% 0.00137 218454
[View Peptides]   Reverse_YJL034W KAR2 SGDID:S000003571, Chr X from 381243-383291, Verified ORF, "ATPase involved in protein import in... 2 4 3.4% 0.00215 74468
[View Peptides]   BBP1 Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periph... 1 1 3.4% 9.52E-4 45384
[View Peptides]   Reverse_YGL173C KEM1 SGDID:S000003141, Chr VII from 180119-175533, reverse complement, Verified ORF, "5'-3' exonucle... 3 3 3.3% 7.19E-4 175459
[View Peptides]   Reverse_YNL209W SSB2 SGDID:S000005153, Chr XIV from 252060-253901, Verified ORF, "Cytoplasmic ATPase that is a ribos... 1 1 3.3% 5.97E-4 66595
[View Peptides]   Reverse_YDL229W SSB1 SGDID:S000002388, Chr IV from 44066-45907, Verified ORF, "Cytoplasmic ATPase that is a ribosome... 1 1 3.3% 5.97E-4 66602
[View Peptides]   Reverse_YLR441C,... RPS1A SGDID:S000004433, Chr XII from 1018904-1018137, reverse complement, Verified ORF, "Ribosomal p... 1 1 3.1% 0.00143 28743
[View Peptides]   Reverse_YKR095W MLP1 SGDID:S000001803, Chr XI from 619447-625074, Verified ORF, "Myosin-like protein associated with... 4 4 3.0% 7.81E-4 218455
[View Peptides]   Reverse_YIL149C MLP2 SGDID:S000001411, Chr IX from 68067-63028, reverse complement, Verified ORF, "Myosin-like prote... 4 5 3.0% 0.00109 195140
[View Peptides]   Reverse_YCL054W SPB1 SGDID:S000000559, Chr III from 31449-33974, Verified ORF, "AdoMet-dependent methyltransferase i... 2 6 3.0% 0.00261 96485
[View Peptides]   HSL7 Protein arginine N-methyltransferase that exhibits septin and Hsl1p-dependent bud neck localization ... 1 1 2.9% 4.43E-4 95153
[View Peptides]   TCP1 Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; inv... 2 3 2.9% 0.00197 60481
[View Peptides]   Reverse_YLR150W STM1 SGDID:S000004140, Chr XII from 440468-441289, Verified ORF, "Protein that binds G4 quadruplex a... 1 1 2.9% 0.00134 29995
[View Peptides]   RPL5 Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 rib... 1 2 2.7% 0.00247 33715
[View Peptides]   NUD1 Component of the spindle pole body outer plaque, required for exit from mitosis 1 1 2.7% 4.31E-4 94104
[View Peptides]   Reverse_YDR172W SUP35 SGDID:S000002579, Chr IV from 808319-810376, Verified ORF, "Translation termination factor eRF... 1 1 2.5% 5.34E-4 76551
[View Peptides]   SPC72 Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them... 1 1 2.4% 5.89E-4 72105
[View Peptides]   HEF3 Translational elongation factor EF-3; paralog of YEF3 and member of the ABC superfamily; stimulates ... 2 8 2.3% 0.00281 115869
[View Peptides]   Reverse_YMR129W POM152 SGDID:S000004736, Chr XIII from 527803-531816, Verified ORF, "Nuclear pore membrane glycoprot... 3 4 2.2% 0.0011 151652
[View Peptides]   Reverse_YNL126W SPC98 SGDID:S000005070, Chr XIV from 387229-389769, Verified ORF, "Component of the microtubule-nucl... 1 1 2.1% 4.33E-4 98227
[View Peptides]   Reverse_YAL047C SPC72 SGDID:S000000045, Chr I from 56858-54990, reverse complement, Verified ORF, "Component of the ... 1 1 2.1% 5.88E-4 72105
[View Peptides]   Reverse_YAL005C SSA1 SGDID:S000000004, Chr I from 141433-139505, reverse complement, Verified ORF, "ATPase involved ... 1 2 1.6% 0.00114 69767
[View Peptides]   KAR1 Essential protein involved in karyogamy during mating and in spindle pole body duplication during mi... 1 1 1.6% 8.46E-4 50653
[View Peptides]   Reverse_YDR356W SPC110 SGDID:S000002764, Chr IV from 1186098-1188932, Verified ORF, "Inner plaque spindle pole body ... 2 2 1.6% 7.76E-4 111782
[View Peptides]   POM152 Nuclear pore membrane glycoprotein; may be involved in duplication of nuclear pores and nuclear pore... 2 2 1.5% 5.48E-4 151652
[View Peptides]   SPC98 Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the... 1 1 1.4% 4.33E-4 98227
[View Peptides]   SUP35 Translation termination factor eRF3; altered protein conformation creates the [PSI(+)] prion, a domi... 1 2 1.2% 0.00107 76551
[View Peptides]   FKS1 Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic sub... 2 5 0.9% 9.77E-4 214849
[View Peptides]   Reverse_YLR342W FKS1 SGDID:S000004334, Chr XII from 809997-815627, Verified ORF, "Catalytic subunit of 1,3-beta-D-gl... 1 1 0.8% 1.95E-4 214849
[View Peptides]   KEM1 Evolutionarily-conserved 5'-3' exonuclease component of cytoplasmic processing (P) bodies involved i... 1 1 0.8% 2.4E-4 175459
[View Peptides]   Reverse_YHR172W SPC97 SGDID:S000001215, Chr VIII from 448335-450806, Verified ORF, "Component of the microtubule-nuc... 1 1 0.7% 4.45E-4 96825

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