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Bait Protein: MLP2
Organism: Saccharomyces cerevisiae
Publication: Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561.
Comments: #28 Asynchronous Prep5-TiO2 Phosphopeptide Enrichment

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UNFILTERED Run Results: [filter]

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  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   SPC42 Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... 95 110 95.6% 0.123 42271
[View Peptides]   SPC29 Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... 65 69 95.3% 0.111 29280
[View Peptides]   YIL002W-A Putative protein of unknown function; identified by expression profiling and mass spectrometry 29 30 92.8% 0.177 7729
[View Peptides]   MLP2 Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... 300 358 89.8% 0.0869 195140
[View Peptides]   CMD1 Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... 12 15 85.0% 0.0416 16135
[View Peptides]   SPC110 Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... 164 193 83.5% 0.0833 111782
[View Peptides]   CDC31 Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication i... 19 20 81.4% 0.0506 18751
[View Peptides]   CNM67 Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... 82 97 73.5% 0.068 67400
[View Peptides]   BFA1 Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pa... 35 38 63.8% 0.027 66087
[View Peptides]   NBP1 Spindle pole body (SPB) component, required for the insertion of the duplication plaque into the nuc... 16 16 55.8% 0.0204 37354
[View Peptides]   NUD1 Component of the spindle pole body outer plaque, required for exit from mitosis 58 61 41.2% 0.0292 94104
[View Peptides]   RPS31 Fusion protein that is cleaved to yield a ribosomal protein of the small (40S) subunit and ubiquitin... 2 2 35.5% 0.00536 17216
[View Peptides]   Reverse_YML026C,... RPS18A SGDID:S000002858, Chr IV from 1359913-1359959,1360395-1360788, Verified ORF, "Protein compone... 2 2 32.2% 0.00554 17038
[View Peptides]   MPS2 Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required f... 9 9 29.2% 0.00947 44585
[View Peptides]   RPS18B, RPS18A Protein component of the small (40S) ribosomal subunit; nearly identical to Rps18Ap and has similari... 5 5 28.8% 0.014 17038
[View Peptides]   RPL5 Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 rib... 6 6 28.3% 0.00823 33715
[View Peptides]   BBP1 Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periph... 12 12 27.5% 0.0127 45384
[View Peptides]   KAR2 ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... 6 6 22.3% 0.00358 74468
[View Peptides]   STM1 Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with r... 3 3 21.2% 0.00448 29995
[View Peptides]   Reverse_YLR150W STM1 SGDID:S000004140, Chr XII from 440468-441289, Verified ORF, "Protein that binds G4 quadruplex a... 4 4 20.1% 0.00595 29995
[View Peptides]   Reverse_YKR094C,... RPL40A SGDID:S000001410, Chr IX from 68708-68715,69150-69528, Verified ORF, "Fusion protein, identic... 1 1 19.5% 0.00316 14554
[View Peptides]   SPC72 Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them... 7 7 19.0% 0.00458 72105
[View Peptides]   RPS1A Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Bp and has similarit... 2 2 18.0% 0.0032 28743
[View Peptides]   RPL10 Protein component of the large (60S) ribosomal subunit, responsible for joining the 40S and 60S subu... 2 2 16.3% 0.00369 25361
[View Peptides]   PMA1 Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... 4 4 13.7% 0.00178 99619
[View Peptides]   Reverse_YCL054W SPB1 SGDID:S000000559, Chr III from 31449-33974, Verified ORF, "AdoMet-dependent methyltransferase i... 5 5 13.3% 0.00242 96485
[View Peptides]   KAR1 Essential protein involved in karyogamy during mating and in spindle pole body duplication during mi... 4 4 13.2% 0.00376 50653
[View Peptides]   RPP2A Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction... 1 1 13.2% 0.00384 10746
[View Peptides]   MLP1 Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... 16 16 12.9% 0.00348 218454
[View Peptides]   Reverse_YDR172W SUP35 SGDID:S000002579, Chr IV from 808319-810376, Verified ORF, "Translation termination factor eRF... 2 2 12.7% 0.00119 76551
[View Peptides]   Reverse_YGL075C MPS2 SGDID:S000003043, Chr VII from 368091-366928, reverse complement, Verified ORF, "Essential memb... 2 2 12.7% 0.0021 44586
[View Peptides]   TUB4 Gamma-tubulin, involved in nucleating microtubules from both the cytoplasmic and nuclear faces of th... 2 2 12.3% 0.00172 52628
[View Peptides]   SSB2 Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... 3 3 11.9% 0.00199 66595
[View Peptides]   Reverse_YOL039W RPP2A SGDID:S000005399, Chr XV from 254295-254615, Verified ORF, "Ribosomal protein P2 alpha, a comp... 1 1 11.3% 0.00381 10746
[View Peptides]   Reverse_YDL229W SSB1 SGDID:S000002388, Chr IV from 44066-45907, Verified ORF, "Cytoplasmic ATPase that is a ribosome... 2 2 11.1% 0.00133 66602
[View Peptides]   Reverse_YNL209W SSB2 SGDID:S000005153, Chr XIV from 252060-253901, Verified ORF, "Cytoplasmic ATPase that is a ribos... 2 2 11.1% 0.00133 66595
[View Peptides]   Reverse_YGL173C KEM1 SGDID:S000003141, Chr VII from 180119-175533, reverse complement, Verified ORF, "5'-3' exonucle... 6 6 10.9% 0.0016 175459
[View Peptides]   EFT2, EFT1 Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein s... 3 3 10.7% 0.00145 93289
[View Peptides]   Reverse_YDR356W SPC110 SGDID:S000002764, Chr IV from 1186098-1188932, Verified ORF, "Inner plaque spindle pole body ... 6 6 10.3% 0.00259 111782
[View Peptides]   Reverse_YPL124W SPC29 SGDID:S000006045, Chr XVI from 316754-317515, Verified ORF, "Inner plaque spindle pole body (S... 2 2 10.3% 0.00321 29280
[View Peptides]   Reverse_YPL255W BBP1 SGDID:S000006176, Chr XVI from 67725-68882, Verified ORF, "Protein required for the spindle pol... 2 2 10.1% 0.00211 45384
[View Peptides]   Reverse_YLR167W RPS31 SGDID:S000004157, Chr XII from 498949-499407, Verified ORF, "Fusion protein that is cleaved to... 1 1 9.9% 0.00266 17216
[View Peptides]   Reverse_YAL047C SPC72 SGDID:S000000045, Chr I from 56858-54990, reverse complement, Verified ORF, "Component of the ... 4 4 9.6% 0.00262 72105
[View Peptides]   Reverse_YNL126W SPC98 SGDID:S000005070, Chr XIV from 387229-389769, Verified ORF, "Component of the microtubule-nucl... 2 2 9.3% 9.62E-4 98227
[View Peptides]   SPC98 Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the... 4 4 9.0% 0.00193 98227
[View Peptides]   Reverse_YLR441C,... RPS1A SGDID:S000004433, Chr XII from 1018904-1018137, reverse complement, Verified ORF, "Ribosomal p... 1 1 8.6% 0.00159 28743
[View Peptides]   Reverse_YML063W,... RPS1B SGDID:S000004528, Chr XIII from 146482-147249, Verified ORF, "Ribosomal protein 10 (rp10) of t... 1 1 8.6% 0.00159 28812
[View Peptides]   Reverse_YPL131W,... RPL5 SGDID:S000006052, Chr XVI from 303120-304013, Verified ORF, "Protein component of the large (60... 1 1 8.4% 0.00137 33715
[View Peptides]   POM152 Nuclear pore membrane glycoprotein; may be involved in duplication of nuclear pores and nuclear pore... 4 4 8.3% 0.00122 151652
[View Peptides]   Reverse_YNL014W HEF3 SGDID:S000004959, Chr XIV from 606320-609454, Verified ORF, "Translational elongation factor EF... 4 4 8.1% 0.00156 115868
[View Peptides]   KEM1 Evolutionarily-conserved 5'-3' exonuclease component of cytoplasmic processing (P) bodies involved i... 7 7 7.9% 0.00187 175459
[View Peptides]   YTM1 Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required fo... 2 2 7.8% 0.00177 51359
[View Peptides]   HSC82 Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, a... 4 4 7.7% 0.00231 80900
[View Peptides]   Reverse_YOR373W NUD1 SGDID:S000005900, Chr XV from 1036830-1039385, Verified ORF, "Component of the spindle pole bod... 3 3 7.6% 0.00143 94104
[View Peptides]   Reverse_YKR095W MLP1 SGDID:S000001803, Chr XI from 619447-625074, Verified ORF, "Myosin-like protein associated with... 10 10 7.6% 0.00217 218455
[View Peptides]   Reverse_YJR053W BFA1 SGDID:S000003814, Chr X from 533941-535665, Verified ORF, "Component of the GTPase-activating B... 3 3 7.3% 0.00213 66087
[View Peptides]   Reverse_YLR457C NBP1 SGDID:S000004449, Chr XII from 1056768-1055809, reverse complement, Verified ORF, "Component of... 2 2 6.9% 0.00255 37354
[View Peptides]   Reverse_YMR186W HSC82 SGDID:S000004798, Chr XIII from 632354-634471, Verified ORF, "Cytoplasmic chaperone of the Hsp... 2 2 6.8% 0.00115 80900
[View Peptides]   Reverse_YKL042W SPC42 SGDID:S000001525, Chr XI from 358119-359210, Verified ORF, "Central plaque component of spindl... 2 2 6.6% 0.00224 42271
[View Peptides]   Reverse_YDR385W,... EFT2 SGDID:S000002793, Chr IV from 1243220-1245748, Verified ORF, "Elongation factor 2 (EF-2), also ... 3 3 6.4% 0.00145 93289
[View Peptides]   HEF3 Translational elongation factor EF-3; paralog of YEF3 and member of the ABC superfamily; stimulates ... 3 3 6.1% 0.00117 115869
[View Peptides]   SPC97 Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the... 3 3 5.8% 0.00148 96825
[View Peptides]   Reverse_YNL188W KAR1 SGDID:S000005132, Chr XIV from 286309-287610, Verified ORF, "Essential protein involved in kary... 1 1 4.8% 9.39E-4 50653
[View Peptides]   Reverse_YLR075W RPL10 SGDID:S000004065, Chr XII from 282928-283593, Verified ORF, "Protein component of the large (6... 1 1 4.5% 0.00184 25361
[View Peptides]   Reverse_YMR129W POM152 SGDID:S000004736, Chr XIII from 527803-531816, Verified ORF, "Nuclear pore membrane glycoprot... 2 2 4.2% 6.09E-4 151652
[View Peptides]   ENO2 Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... 1 1 4.1% 9.32E-4 46914
[View Peptides]   Reverse_YIL149C MLP2 SGDID:S000001411, Chr IX from 68067-63028, reverse complement, Verified ORF, "Myosin-like prote... 5 5 4.0% 0.00121 195140
[View Peptides]   Reverse_YJR002W MPP10 SGDID:S000003762, Chr X from 438779-440560, Verified ORF, "Component of the SSU processome, wh... 2 2 3.9% 0.00137 66953
[View Peptides]   MPP10 Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts... 1 1 3.9% 6.87E-4 66953
[View Peptides]   Reverse_YLR342W FKS1 SGDID:S000004334, Chr XII from 809997-815627, Verified ORF, "Catalytic subunit of 1,3-beta-D-gl... 3 3 3.7% 6.51E-4 214849
[View Peptides]   SPB1 AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation;... 3 3 3.2% 0.00145 96485
[View Peptides]   SSA1 ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport;... 3 3 3.1% 0.0019 69768
[View Peptides]   Reverse_YNL225C CNM67 SGDID:S000005169, Chr XIV from 224470-222725, reverse complement, Verified ORF, "Component of ... 2 2 3.1% 0.0014 67400
[View Peptides]   HSL7 Protein arginine N-methyltransferase that exhibits septin and Hsl1p-dependent bud neck localization ... 1 1 2.9% 4.93E-4 95153
[View Peptides]   FKS1 Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic sub... 2 2 2.1% 4.34E-4 214849
[View Peptides]   Reverse_YDR212W TCP1 SGDID:S000002620, Chr IV from 887227-888906, Verified ORF, "Alpha subunit of chaperonin-contain... 1 1 2.1% 7.27E-4 60481
[View Peptides]   Reverse_YHR174W ENO2 SGDID:S000001217, Chr VIII from 451327-452640, Verified ORF, "Enolase II, a phosphopyruvate hyd... 1 1 1.8% 9.3E-4 46914
[View Peptides]   Reverse_YGR254W ENO1 SGDID:S000003486, Chr VII from 1000932-1002245, Verified ORF, "Enolase I, a phosphopyruvate hyd... 1 1 1.8% 9.3E-4 46816
[View Peptides]   Reverse_YAL005C SSA1 SGDID:S000000004, Chr I from 141433-139505, reverse complement, Verified ORF, "ATPase involved ... 1 1 1.7% 6.34E-4 69767
[View Peptides]   PDC1 Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates... 1 1 1.6% 7.24E-4 61495
[View Peptides]   SUP35 Translation termination factor eRF3; altered protein conformation creates the [PSI(+)] prion, a domi... 1 1 1.2% 5.95E-4 76551
[View Peptides]   Reverse_YJL034W KAR2 SGDID:S000003571, Chr X from 381243-383291, Verified ORF, "ATPase involved in protein import in... 2 2 1.2% 0.00119 74468
[View Peptides]   Reverse_YHR172W SPC97 SGDID:S000001215, Chr VIII from 448335-450806, Verified ORF, "Component of the microtubule-nuc... 1 1 1.1% 4.94E-4 96825
[View Peptides]   Reverse_YGL008C PMA1 SGDID:S000002976, Chr VII from 482671-479915, reverse complement, Verified ORF, "Plasma membran... 1 1 0.9% 4.43E-4 99619
YRC Informatics Platform - Version 3.0
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