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Run Information:


Bait Protein: MLP2
Organism: Saccharomyces cerevisiae
Publication: Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561.
Comments: #27 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps3-5

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UNFILTERED Run Results: [filter]

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  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   CDC31 Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication i... 3 8 40.4% 0.252 18751
[View Peptides]   CMD1 Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... 2 6 25.2% 0.207 16135
[View Peptides]   RDL2 Protein with rhodanese activity; contains a rhodanese-like domain similar to Rdl1p, Uba4p, Tum1p, an... 1 1 18.8% 0.034 16697
[View Peptides]   SPC29 Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... 4 5 15.0% 0.1 29280
[View Peptides]   SNP1 Component of U1 snRNP required for mRNA splicing via spliceosome; may interact with poly(A) polymera... 1 1 13.3% 0.0169 34447
[View Peptides]   CDC14 Protein phosphatase required for mitotic exit; located in the nucleolus until liberated by the FEAR ... 2 2 12.7% 0.0184 61907
[View Peptides]   FBA1 Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversio... 1 1 12.3% 0.0141 39621
[View Peptides]   RPL5 Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 rib... 1 3 10.4% 0.0512 33715
[View Peptides]   YNR040W Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purifi... 1 1 9.4% 0.0198 28636
[View Peptides]   Reverse_YBR192W RIM2 SGDID:S000000396, Chr II from 607647-608780, Verified ORF, "Mitochondrial pyrimidine nucleotide... 1 1 9.3% 0.0134 42102
[View Peptides]   MID2 O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and a... 1 1 8.2% 0.0135 39146
[View Peptides]   YDJ1 Protein chaperone involved in regulation of the HSP90 and HSP70 functions; involved in protein trans... 1 1 8.1% 0.0124 44671
[View Peptides]   Reverse_YKL141W SDH3 SGDID:S000001624, Chr XI from 179672-180268, Verified ORF, "Cytochrome b subunit of succinate d... 1 1 8.1% 0.0255 22068
[View Peptides]   MPS2 Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required f... 1 1 7.2% 0.0131 44585
[View Peptides]   CNM67 Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... 1 2 5.9% 0.0175 67400
[View Peptides]   YHB1 Nitric oxide oxidoreductase, flavohemoglobin involved in nitric oxide detoxification; plays a role i... 1 1 5.3% 0.0127 44646
[View Peptides]   BFA1 Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pa... 3 4 5.2% 0.0354 66087
[View Peptides]   Reverse_YBL097W BRN1 SGDID:S000000193, Chr II from 40828-43092, Verified ORF, "Essential protein required for chromo... 1 1 3.7% 0.00672 86230
[View Peptides]   SMF1 Divalent metal ion transporter with a broad specificity for di-valent and tri-valent metals; post-tr... 1 1 3.3% 0.00882 63264
[View Peptides]   PMC1 Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activ... 1 1 3.0% 0.00433 130861
[View Peptides]   MDL1 Mitochondrial inner membrane half-type ATP-binding cassette (ABC) transporter, mediates export of pe... 1 1 3.0% 0.0073 75950
[View Peptides]   Reverse_YOL087C YOL087C SGDID:S000005447, Chr XV from 158636-155286, reverse complement, Uncharacterized ORF, "Hypot... 1 1 2.9% 0.00454 125382
[View Peptides]   HSL7 Protein arginine N-methyltransferase that exhibits septin and Hsl1p-dependent bud neck localization ... 1 1 2.7% 0.00613 95153
[View Peptides]   SPC72 Component of the cytoplasmic Tub4p (gamma-tubulin) complex, binds spindle pole bodies and links them... 1 1 2.7% 0.00816 72105
[View Peptides]   RAT1 Nuclear 5' to 3' single-stranded RNA exonuclease, involved in RNA metabolism, including rRNA and snR... 1 1 2.4% 0.00504 115934
[View Peptides]   NUD1 Component of the spindle pole body outer plaque, required for exit from mitosis 2 8 2.2% 0.0477 94104
[View Peptides]   MSS11 Transcription factor involved in regulation of invasive growth and starch degradation; controls the ... 1 1 2.2% 0.00669 85050
[View Peptides]   YCF1 Vacuolar glutathione S-conjugate transporter of the ATP-binding cassette family, has a role in detox... 1 1 2.0% 0.00335 171120
[View Peptides]   VPS5 Nexin-1 homolog required for localizing membrane proteins from a prevacuolar/late endosomal compartm... 1 1 1.9% 0.00752 76484
[View Peptides]   YLR413W Putative protein of unknown function; YLR413W is not an essential gene 1 1 1.8% 0.00752 72698
[View Peptides]   AIP1 Actin cortical patch component, interacts with the actin depolymerizing factor cofilin; required to ... 1 1 1.5% 0.00825 67326
[View Peptides]   PDR12 Plasma membrane ATP-binding cassette (ABC) transporter, weak-acid-inducible multidrug transporter re... 1 1 1.1% 0.00336 171064
[View Peptides]   TRM3 2'-O-ribose methyltransferase, catalyzes the ribose methylation of the guanosine nucleotide at posit... 1 1 1.1% 0.00353 165047
[View Peptides]   ECM5 Non-essential protein of unknown function, contains ATP/GTP-binding site motif A; null mutant exhibi... 1 1 0.6% 0.0036 162700

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