Bait Protein: | MLP2 |
Organism: | Saccharomyces cerevisiae |
Publication: | Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561. |
Comments: | #21 Asynchronous Prep2-IMAC Phosphopeptide enrichment A1 |
Raw Data: |
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UNFILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
[ |
CMD1 | Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... | 5 | 6 | 49.7% | 0.119 | 16135 |
[ |
SPC42 | Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... | 15 | 17 | 44.4% | 0.136 | 42271 |
[ |
YIL002W-A | Putative protein of unknown function; identified by expression profiling and mass spectrometry | 1 | 1 | 34.8% | 0.0421 | 7729 |
[ |
SPC29 | Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... | 6 | 7 | 28.1% | 0.0804 | 29280 |
[ |
CNM67 | Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... | 16 | 17 | 26.9% | 0.085 | 67400 |
[ |
HHT1, HHT2 | Histone H3, core histone protein required for chromatin assembly, part of heterochromatin-mediated t... | 1 | 1 | 23.5% | 0.0214 | 15356 |
[ |
MLP2 | Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... | 25 | 25 | 18.2% | 0.0433 | 195140 |
[ |
NUD1 | Component of the spindle pole body outer plaque, required for exit from mitosis | 12 | 12 | 18.2% | 0.041 | 94104 |
[ |
CDC31 | Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication i... | 2 | 2 | 14.9% | 0.0361 | 18751 |
[ |
RPS7A | Protein component of the small (40S) ribosomal subunit, nearly identical to Rps7Bp; interacts with K... | 1 | 1 | 14.7% | 0.0153 | 21622 |
[ |
TDH3 | Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3, involved in glycolysis and gluconeogenesis; tet... | 2 | 2 | 13.3% | 0.0175 | 35747 |
[ |
BFA1 | Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pa... | 4 | 4 | 10.1% | 0.0202 | 66087 |
[ |
RPL5 | Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 rib... | 1 | 1 | 9.4% | 0.00978 | 33715 |
[ |
NSR1 | Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ri... | 1 | 1 | 7.7% | 0.00702 | 44535 |
[ |
Reverse_YOR292C | YOR292C SGDID:S000005818, Chr XV from 866579-865650, reverse complement, Uncharacterized ORF, "Hypot... | 1 | 1 | 7.1% | 0.00937 | 36195 |
[ |
RPS3 | Protein component of the small (40S) ribosomal subunit, has apurinic/apyrimidinic (AP) endonuclease ... | 1 | 1 | 7.1% | 0.0121 | 26503 |
[ |
SRX1 | Sulfiredoxin, contributes to oxidative stress resistance by reducing cysteine-sulfinic acid groups i... | 1 | 1 | 7.1% | 0.0229 | 13854 |
[ |
RPS12 | Protein component of the small (40S) ribosomal subunit; has similarity to rat ribosomal protein S12 | 1 | 1 | 7.0% | 0.0203 | 15472 |
[ |
RRN3 | Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of ... | 2 | 2 | 6.7% | 0.00927 | 72388 |
[ |
SSA2 | ATP binding protein involved in protein folding and vacuolar import of proteins; member of heat shoc... | 2 | 2 | 6.7% | 0.00909 | 69470 |
[ |
PUP1 | Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after ba... | 1 | 1 | 5.4% | 0.0111 | 28268 |
[ |
RPL17A | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl17Bp and has similari... | 1 | 1 | 5.4% | 0.0158 | 20549 |
[ |
RPL19B, RPL19A | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl19Ap and has similari... | 1 | 1 | 5.3% | 0.0154 | 21704 |
[ |
DAK2 | Dihydroxyacetone kinase, required for detoxification of dihydroxyacetone (DHA); involved in stress a... | 1 | 1 | 5.2% | 0.00492 | 62135 |
[ |
Reverse_YDR305C | HNT2 SGDID:S000002713, Chr IV from 1073482-1073396,1073306-1072740, reverse complement, Verified ORF... | 1 | 1 | 4.6% | 0.0133 | 24784 |
[ |
BBP1 | Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periph... | 1 | 1 | 4.4% | 0.00755 | 45384 |
[ |
ECM31 | Ketopantoate hydroxymethyltransferase, required for pantothenic acid biosynthesis, converts 2-oxoiso... | 1 | 1 | 4.2% | 0.00931 | 34465 |
[ |
Reverse_YOR323C | PRO2 SGDID:S000005850, Chr XV from 922900-921530, reverse complement, Verified ORF, "Gamma-glutamyl ... | 1 | 1 | 3.7% | 0.00636 | 49741 |
[ |
MFB1 | Mitochondria-associated F-box protein involved in maintenance of normal mitochondrial morphology; in... | 1 | 1 | 3.2% | 0.00625 | 53168 |
[ |
VPS4 | AAA-ATPase involved in multivesicular body (MVB) protein sorting, ATP-bound Vps4p localizes to endos... | 1 | 1 | 3.2% | 0.00665 | 48172 |
[ |
Reverse_YLR052W | IES3 SGDID:S000004042, Chr XII from 247202-247954, Verified ORF, "Subunit of the INO80 chromatin rem... | 1 | 1 | 3.2% | 0.0116 | 28243 |
[ |
Reverse_YIL131C | FKH1 SGDID:S000001393, Chr IX from 102235-100781, reverse complement, Verified ORF, "Transcription f... | 1 | 1 | 3.1% | 0.00599 | 53490 |
[ |
Reverse_YHR064C | SSZ1 SGDID:S000001106, Chr VIII from 227143-225527, reverse complement, Verified ORF, "Hsp70 protein... | 1 | 1 | 2.6% | 0.00539 | 58238 |
[ |
ALK2 | Protein kinase; accumulation and phosphorylation are periodic during the cell cycle; phosphorylated ... | 1 | 1 | 2.5% | 0.0043 | 76372 |
[ |
QNS1 | Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid ade... | 1 | 1 | 2.4% | 0.00407 | 80686 |
[ |
NOP1 | Nucleolar protein, component of the small subunit processome complex, which is required for processi... | 1 | 1 | 2.4% | 0.00889 | 34465 |
[ |
SPC110 | Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... | 2 | 3 | 2.1% | 0.00923 | 111782 |
[ |
SWP82 | Member of the SWI/SNF chromatin remodeling complex in which it plays an as yet unidentified role; ha... | 1 | 1 | 2.1% | 0.00466 | 70276 |
[ |
SAN1 | Ubiquitin-protein ligase, involved in the proteasome-dependent degradation of aberrant nuclear prote... | 1 | 1 | 2.1% | 0.00476 | 65618 |
[ |
APM1 | Mu1-like medium subunit of the clathrin-associated protein complex (AP-1); binds clathrin; involved ... | 1 | 1 | 2.1% | 0.00612 | 53874 |
[ |
TDA11 | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to th... | 1 | 1 | 2.0% | 0.00577 | 56314 |
[ |
YGR012W | Putative cysteine synthase, localized to the mitochondrial outer membrane | 1 | 1 | 2.0% | 0.00739 | 42801 |
[ |
Reverse_YML035C | AMD1 SGDID:S000004498, Chr XIII from 208860-206428, reverse complement, Verified ORF, "AMP deaminase... | 1 | 1 | 1.9% | 0.00358 | 93302 |
[ |
Reverse_YDR085C | AFR1 SGDID:S000002492, Chr IV from 616145-614283, reverse complement, Verified ORF, "Alpha-factor ph... | 1 | 1 | 1.9% | 0.00468 | 71364 |
[ |
Reverse_YGR260W | TNA1 SGDID:S000003492, Chr VII from 1012491-1014095, Verified ORF, "High affinity nicotinic acid pla... | 1 | 1 | 1.9% | 0.00543 | 60135 |
[ |
ROG3 | Protein that binds the ubiquitin ligase Rsp5p via its 2 PY motifs; has similarity to Rod1p; mutation... | 1 | 1 | 1.8% | 0.00396 | 79709 |
[ |
TOS1 | Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF tar... | 1 | 1 | 1.8% | 0.00639 | 47994 |
[ |
FRE1 | Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to u... | 1 | 1 | 1.6% | 0.00424 | 78854 |
[ |
IQG1 | Essential protein required for determination of budding pattern, promotes localization of axial mark... | 1 | 1 | 1.5% | 0.00194 | 172830 |
[ |
MSB2 | Mucin family member involved in the Cdc42p- and MAP kinase-dependent filamentous growth signaling pa... | 1 | 1 | 1.5% | 0.00222 | 133114 |
[ |
Reverse_YOL089C | HAL9 SGDID:S000005449, Chr XV from 153489-150397, reverse complement, Verified ORF, "Putative transc... | 1 | 1 | 1.5% | 0.00282 | 117926 |
[ |
UTP25 | Nucleolar protein that cosediments with the 40S ribosomal subunit and is required for its biogenesis... | 1 | 1 | 1.5% | 0.00403 | 83991 |
[ |
TDA9 | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to th... | 1 | 1 | 1.4% | 0.00232 | 141465 |
[ |
RAD3 | 5' to 3' DNA helicase, involved in nucleotide excision repair and transcription; subunit of RNA poly... | 1 | 1 | 1.3% | 0.00373 | 89786 |
[ |
SAS10 | Essential subunit of U3-containing Small Subunit (SSU) processome complex involved in production of ... | 1 | 1 | 1.3% | 0.00476 | 70260 |
[ |
HER1 | Protein of unknown function required for proliferation or remodeling of the ER that is caused by ove... | 1 | 1 | 1.1% | 0.00233 | 139457 |
[ |
VRP1 | Proline-rich actin-associated protein involved in cytoskeletal organization and cytokinesis; related... | 1 | 1 | 1.1% | 0.00356 | 82594 |
[ |
Reverse_YKR103W | NFT1 SGDID:S000001811, Chr XI from 652718-656374, Verified ORF, "Putative transporter of the multidr... | 1 | 1 | 1.0% | 0.00238 | 137996 |
[ |
Reverse_YDR379W | RGA2 SGDID:S000002787, Chr IV from 1230157-1233186, Verified ORF, "GTPase-activating protein for the... | 1 | 1 | 1.0% | 0.00288 | 113291 |
[ |
EDE1 | Key endocytic protein involved in a network of interactions with other endocytic proteins, binds mem... | 1 | 1 | 0.8% | 0.0021 | 150783 |
[ |
Reverse_YPR095C | SYT1 SGDID:S000006299, Chr XVI from 724715-721035, reverse complement, Verified ORF, "Guanine nucleo... | 1 | 1 | 0.7% | 0.00237 | 137583 |
[ |
Reverse_YMR207C | HFA1 SGDID:S000004820, Chr XIII from 683563-677192, reverse complement, Verified ORF, "Mitochondrial... | 1 | 1 | 0.6% | 0.00137 | 241784 |
[ |
Reverse_YNR016C | ACC1 SGDID:S000005299, Chr XIV from 661376-654675, reverse complement, Verified ORF, "Acetyl-CoA car... | 1 | 1 | 0.5% | 0.0013 | 250351 |