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Bait Protein: MLP2
Organism: Saccharomyces cerevisiae
Publication: Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561.
Comments: #07 Alpha Factor Prep3-TiO2 Flowthrough

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  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   MLP2 Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... 184 220 69.6% 0.127 195140
[View Peptides]   YIL002W-A Putative protein of unknown function; identified by expression profiling and mass spectrometry 8 11 65.2% 0.155 7729
[View Peptides]   SPC42 Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate att... 11 11 42.4% 0.0295 42271
[View Peptides]   SSB1 Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... 19 19 42.4% 0.0301 66602
[View Peptides]   RPS1A Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Bp and has similarit... 9 9 40.8% 0.0343 28743
[View Peptides]   SSB2 Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... 18 18 40.3% 0.0285 66595
[View Peptides]   PDC1 Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates... 16 19 36.9% 0.0328 61495
[View Peptides]   RPS1B Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Ap and has similarit... 9 9 35.7% 0.0343 28812
[View Peptides]   RPS18B, RPS18A Protein component of the small (40S) ribosomal subunit; nearly identical to Rps18Ap and has similari... 6 7 34.2% 0.0466 17038
[View Peptides]   RPL5 Protein component of the large (60S) ribosomal subunit with similarity to E. coli L18 and rat L5 rib... 9 9 33.7% 0.0295 33715
[View Peptides]   RPL13B Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl13Ap; not essential f... 8 8 33.7% 0.0391 22525
[View Peptides]   RPP2A Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction... 2 2 29.2% 0.0183 10746
[View Peptides]   ENO2 Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... 9 9 28.8% 0.02 46914
[View Peptides]   SPC29 Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inn... 6 6 28.5% 0.0231 29280
[View Peptides]   SSA1 ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport;... 16 17 27.6% 0.0257 69768
[View Peptides]   RPL10 Protein component of the large (60S) ribosomal subunit, responsible for joining the 40S and 60S subu... 6 6 26.2% 0.0264 25361
[View Peptides]   CDC31 Calcium-binding component of the spindle pole body (SPB) half-bridge, required for SPB duplication i... 3 3 26.1% 0.0181 18751
[View Peptides]   CMD1 Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... 3 3 24.5% 0.0198 16135
[View Peptides]   EFT2, EFT1 Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein s... 18 18 23.4% 0.0208 93289
[View Peptides]   SPC110 Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... 17 17 20.0% 0.0175 111782
[View Peptides]   PMA1 Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... 12 13 19.5% 0.0138 99619
[View Peptides]   STM1 Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with r... 4 4 19.4% 0.0142 29995
[View Peptides]   ENO1 Enolase I, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosph... 6 6 18.8% 0.0133 46816
[View Peptides]   CNM67 Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclea... 5 5 11.7% 0.00837 67400
[View Peptides]   KAR2 ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... 4 4 10.6% 0.0057 74468
[View Peptides]   Reverse_YLR457C NBP1 SGDID:S000004449, Chr XII from 1056768-1055809, reverse complement, Verified ORF, "Component of... 2 2 10.0% 0.00608 37354
[View Peptides]   Reverse_YLR150W STM1 SGDID:S000004140, Chr XII from 440468-441289, Verified ORF, "Protein that binds G4 quadruplex a... 2 2 9.5% 0.0071 29995
[View Peptides]   NBP1 Spindle pole body (SPB) component, required for the insertion of the duplication plaque into the nuc... 4 4 9.4% 0.0122 37354
[View Peptides]   MPS2 Essential membrane protein localized at the nuclear envelope and spindle pole body (SPB), required f... 3 3 9.0% 0.00754 44585
[View Peptides]   BBP1 Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periph... 2 2 8.1% 0.00505 45384
[View Peptides]   BFA1 Component of the GTPase-activating Bfa1p-Bub2p complex involved in multiple cell cycle checkpoint pa... 6 6 8.0% 0.0102 66087
[View Peptides]   Reverse_YPL131W,... RPL5 SGDID:S000006052, Chr XVI from 303120-304013, Verified ORF, "Protein component of the large (60... 2 2 7.7% 0.00653 33715
[View Peptides]   HSC82 Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, a... 4 4 7.2% 0.00552 80900
[View Peptides]   Reverse_YNL225C CNM67 SGDID:S000005169, Chr XIV from 224470-222725, reverse complement, Verified ORF, "Component of ... 3 3 7.1% 0.00501 67400
[View Peptides]   Reverse_YLR075W RPL10 SGDID:S000004065, Chr XII from 282928-283593, Verified ORF, "Protein component of the large (6... 1 1 6.3% 0.00438 25361
[View Peptides]   MPP10 Component of the SSU processome and 90S preribosome, required for pre-18S rRNA processing, interacts... 3 3 6.2% 0.00492 66953
[View Peptides]   NUD1 Component of the spindle pole body outer plaque, required for exit from mitosis 4 4 6.2% 0.00457 94104
[View Peptides]   Reverse_YPL124W SPC29 SGDID:S000006045, Chr XVI from 316754-317515, Verified ORF, "Inner plaque spindle pole body (S... 1 1 5.9% 0.00383 29280
[View Peptides]   Reverse_YML026C,... RPS18A SGDID:S000002858, Chr IV from 1359913-1359959,1360395-1360788, Verified ORF, "Protein compone... 1 1 5.5% 0.00661 17038
[View Peptides]   RPS31 Fusion protein that is cleaved to yield a ribosomal protein of the small (40S) subunit and ubiquitin... 1 1 5.3% 0.0064 17216
[View Peptides]   SPC97 Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the... 3 3 5.2% 0.00354 96825
[View Peptides]   KAR1 Essential protein involved in karyogamy during mating and in spindle pole body duplication during mi... 1 1 5.1% 0.00225 50653
[View Peptides]   TCP1 Alpha subunit of chaperonin-containing T-complex, which mediates protein folding in the cytosol; inv... 2 2 5.0% 0.00348 60481
[View Peptides]   HEF3 Translational elongation factor EF-3; paralog of YEF3 and member of the ABC superfamily; stimulates ... 4 4 4.9% 0.00373 115869
[View Peptides]   Reverse_YCL054W SPB1 SGDID:S000000559, Chr III from 31449-33974, Verified ORF, "AdoMet-dependent methyltransferase i... 2 2 4.9% 0.00231 96485
[View Peptides]   Reverse_YPL255W BBP1 SGDID:S000006176, Chr XVI from 67725-68882, Verified ORF, "Protein required for the spindle pol... 1 1 4.9% 0.00252 45384
[View Peptides]   Reverse_YMR142C RPL13B SGDID:S000004750, Chr XIII from 551206-551203,550800-550205, reverse complement, Verified ORF... 1 1 4.5% 0.00486 22525
[View Peptides]   Reverse_YDR356W SPC110 SGDID:S000002764, Chr IV from 1186098-1188932, Verified ORF, "Inner plaque spindle pole body ... 5 5 4.4% 0.00514 111782
[View Peptides]   MLP1 Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... 6 6 4.3% 0.00311 218454
[View Peptides]   Reverse_YKR095W MLP1 SGDID:S000001803, Chr XI from 619447-625074, Verified ORF, "Myosin-like protein associated with... 5 5 4.2% 0.00259 218455
[View Peptides]   Reverse_YMR129W POM152 SGDID:S000004736, Chr XIII from 527803-531816, Verified ORF, "Nuclear pore membrane glycoprot... 4 4 3.8% 0.00291 151652
[View Peptides]   SUP35 Translation termination factor eRF3; altered protein conformation creates the [PSI(+)] prion, a domi... 2 2 3.5% 0.00284 76551
[View Peptides]   FKS1 Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic sub... 4 4 3.3% 0.00207 214849
[View Peptides]   Reverse_YOR272W YTM1 SGDID:S000005798, Chr XV from 832810-834192, Verified ORF, "Constituent of 66S pre-ribosomal pa... 1 1 3.0% 0.00211 51359
[View Peptides]   YTM1 Constituent of 66S pre-ribosomal particles, forms a complex with Nop7p and Erb1p that is required fo... 1 1 2.8% 0.00211 51359
[View Peptides]   SPB1 AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation;... 1 1 2.7% 0.00116 96485
[View Peptides]   Reverse_YBR133C HSL7 SGDID:S000000337, Chr II from 504281-501798, reverse complement, Verified ORF, "Protein arginin... 1 1 2.7% 0.00117 95153
[View Peptides]   Reverse_YMR186W HSC82 SGDID:S000004798, Chr XIII from 632354-634471, Verified ORF, "Cytoplasmic chaperone of the Hsp... 1 1 2.7% 0.00138 80900
[View Peptides]   Reverse_YIL149C MLP2 SGDID:S000001411, Chr IX from 68067-63028, reverse complement, Verified ORF, "Myosin-like prote... 4 4 2.7% 0.00231 195140
[View Peptides]   Reverse_YGL173C KEM1 SGDID:S000003141, Chr VII from 180119-175533, reverse complement, Verified ORF, "5'-3' exonucle... 3 3 2.4% 0.00191 175459
[View Peptides]   Reverse_YDR212W TCP1 SGDID:S000002620, Chr IV from 887227-888906, Verified ORF, "Alpha subunit of chaperonin-contain... 1 1 2.3% 0.00174 60481
[View Peptides]   Reverse_YNL188W KAR1 SGDID:S000005132, Chr XIV from 286309-287610, Verified ORF, "Essential protein involved in kary... 1 1 2.3% 0.00224 50653
[View Peptides]   Reverse_YGL008C PMA1 SGDID:S000002976, Chr VII from 482671-479915, reverse complement, Verified ORF, "Plasma membran... 1 1 2.2% 0.00106 99619
[View Peptides]   KEM1 Evolutionarily-conserved 5'-3' exonuclease component of cytoplasmic processing (P) bodies involved i... 2 2 1.9% 0.00127 175459
[View Peptides]   Reverse_YOR373W NUD1 SGDID:S000005900, Chr XV from 1036830-1039385, Verified ORF, "Component of the spindle pole bod... 2 2 1.8% 0.00228 94104
[View Peptides]   Reverse_YDR385W,... EFT2 SGDID:S000002793, Chr IV from 1243220-1245748, Verified ORF, "Elongation factor 2 (EF-2), also ... 1 1 1.7% 0.00115 93289
[View Peptides]   Reverse_YNL126W SPC98 SGDID:S000005070, Chr XIV from 387229-389769, Verified ORF, "Component of the microtubule-nucl... 1 1 1.4% 0.00115 98227
[View Peptides]   Reverse_YJR053W BFA1 SGDID:S000003814, Chr X from 533941-535665, Verified ORF, "Component of the GTPase-activating B... 1 1 1.4% 0.00169 66087
[View Peptides]   Reverse_YHR172W SPC97 SGDID:S000001215, Chr VIII from 448335-450806, Verified ORF, "Component of the microtubule-nuc... 1 1 1.3% 0.00118 96825
[View Peptides]   HSL7 Protein arginine N-methyltransferase that exhibits septin and Hsl1p-dependent bud neck localization ... 1 1 1.3% 0.00118 95153
[View Peptides]   Reverse_YJR002W MPP10 SGDID:S000003762, Chr X from 438779-440560, Verified ORF, "Component of the SSU processome, wh... 1 1 1.2% 0.00164 66953
[View Peptides]   Reverse_YNL014W HEF3 SGDID:S000004959, Chr XIV from 606320-609454, Verified ORF, "Translational elongation factor EF... 1 1 1.1% 9.3E-4 115868
[View Peptides]   POM152 Nuclear pore membrane glycoprotein; may be involved in duplication of nuclear pores and nuclear pore... 1 1 1.0% 7.27E-4 151652

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