Protein Overview: FKS1

General Information:
Name(s) found:	FKS1 / YLR342W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|683791\|emb\|CAA86404.1\| sensitivity to papulacandin B [Saccharomyces cerevisiae] [NCBI NR] gi\|6323374\|ref\|NP_013446.1\| Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall [NCBI NR] gi\|609385\|gb\|AAB67256.1\| Fks1p [Saccharomyces cerevisiae] [NCBI NR] gi\|577657\|dbj\|BAA07706.1\| Gsc1p [Saccharomyces cerevisiae] [NCBI NR] gi\|577608\|emb\|CAA56783.1\| CWH53 [Saccharomyces cerevisiae] [NCBI NR] gi\|563150\|gb\|AAC48981.1\| 1,3-beta-D-glucan synthase subunit [NCBI NR] gi\|1346146\|sp\|P38631.2\|FKS1_YEAST RecName: Full=1,3-beta-glucan synthase component FKS1; AltName: Full=1,3-beta-D-glucan-UDP glucosyltransferase; AltName: Full=FK506 sensitivity protein 1; AltName: Full=Calcineurin dependent protein 1; AltName: Full=Calco [NCBI NR] pir\|\|S50235 1,3-beta-glucan synthase (EC 2.4.1.34) chain FKS1 - yeast (Saccharomyces cerevisiae) [NCBI NR] 1,3-beta-glucan synthase component GLS1 - Saccharomyces cerevisiae (Baker's yeast) [Swiss-Prot] 1,3-beta-glucan synthase component FKS1 OS=Saccharomyces cerevisiae GN=FKS1 PE=1 SV=2 [Swiss-Prot] FKS1 1,3-beta-D-glucan synthase, catalytic subunit [mips] Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling [SGD] FKS1, Chr XII from 809997-815627 [yeast_orfs2] Chr XII from 809997-815627 [yeastorf3.fasta] (P38631) 1,3-beta-glucan synthase component GLS1 (EC 2.4.1.34) (1,3-beta-D-glucan-UDP glucosyltransferase) (CND1 protein) (CWN53 protein) (FKS1 protein) (Papulacandin B sensitivity protein 1) [4932.FASTACCERV.txt] FKS1 SGDID:S0004334, Chr XII from 809997-815627, Verified ORF [yeastflysequest.fasta] Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling [Saccharomyces c [nrdb_11152004_Chlamydomonas] FKS1 SGDID:S000004334, Chr XII from 809997-815627, Verified ORF, "Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and mainte [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Gsc1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] 1,3-beta-glucan synthase component GLS1 (1,3-beta-D-glucan-UDP glucosyltransferase) (Protein CND1) (Protein CWN53) (Protein FKS1) (Papulacandin B sensitivity protein 1) [nr-271106-contam.fasta] sensitivity to papulacandin B [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling; Fks1p [Saccharo [nr-271106-contam.fasta] Fks1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] CWH53 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] 1,3-beta-D-glucan synthase subunit [nr-271106-contam.fasta] FKS1 SGDID:S000004334, Chr XII from 809997-815627, Verified ORF, "Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling" [yeast-bovrabprots.fasta] [chemostatdb.fasta] 1,3-beta-glucan synthase component FKS1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=FKS1 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] FKS1 SGDID:S000004334, Chr XII from 809997-815627, Genome Release 64-1-1, Verified ORF, "Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling" [yeast-030211-contam.fasta] FKS1 SGDID:S000004334, Chr XII from 809997-815627, Genome Release 64-2-1, Verified ORF, "Catalytic subunit of 1,3-beta-D-glucan synthase; functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling; FKS1 has a paralog, GSC2, that arose from the whole genome duplication" [20141208_orf_trans.fasta] YLR342W SGDID:S000004334, Chr XII from 809997-815627, Genome Release 64-1-1, Verified ORF, "Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	1876 amino acids
Gene Ontology:
Cellular Component:	mitochondrion [IDA] 1,3-beta-glucan synthase complex [TAS] actin cortical patch [IDA] actin cap [IDA]
Biological Process:	1,3-beta-glucan biosynthetic process [TAS] regulation of cell size [IMP] cellular cell wall organization [TAS] endocytosis [IMP]
Molecular Function:	1,3-beta-glucan synthase activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MNTDQQPYQG QTDYTQGPGN GQSQEQDYDQ YGQPLYPSQA DGYYDPNVAA GTEADMYGQQ  60
   61 PPNESYDQDY TNGEYYGQPP NMAAQDGENF SDFSSYGPPG TPGYDSYGGQ YTASQMSYGE 120
  121 PNSSGTSTPI YGNYDPNAIA MALPNEPYPA WTADSQSPVS IEQIEDIFID LTNRLGFQRD 180
  181 SMRNMFDHFM VLLDSRSSRM SPDQALLSLH ADYIGGDTAN YKKWYFAAQL DMDDEIGFRN 240
  241 MSLGKLSRKA RKAKKKNKKA MEEANPEDTE ETLNKIEGDN SLEAADFRWK AKMNQLSPLE 300
  301 RVRHIALYLL CWGEANQVRF TAECLCFIYK CALDYLDSPL CQQRQEPMPE GDFLNRVITP 360
  361 IYHFIRNQVY EIVDGRFVKR ERDHNKIVGY DDLNQLFWYP EGIAKIVLED GTKLIELPLE 420
  421 ERYLRLGDVV WDDVFFKTYK ETRTWLHLVT NFNRIWVMHI SIFWMYFAYN SPTFYTHNYQ 480
  481 QLVDNQPLAA YKWASCALGG TVASLIQIVA TLCEWSFVPR KWAGAQHLSR RFWFLCIIFG 540
  541 INLGPIIFVF AYDKDTVYST AAHVVAAVMF FVAVATIIFF SIMPLGGLFT SYMKKSTRRY 600
  601 VASQTFTAAF APLHGLDRWM SYLVWVTVFA AKYSESYYFL VLSLRDPIRI LSTTAMRCTG 660
  661 EYWWGAVLCK VQPKIVLGLV IATDFILFFL DTYLWYIIVN TIFSVGKSFY LGISILTPWR 720
  721 NIFTRLPKRI YSKILATTDM EIKYKPKVLI SQVWNAIIIS MYREHLLAID HVQKLLYHQV 780
  781 PSEIEGKRTL RAPTFFVSQD DNNFETEFFP RDSEAERRIS FFAQSLSTPI PEPLPVDNMP 840
  841 TFTVLTPHYA ERILLSLREI IREDDQFSRV TLLEYLKQLH PVEWECFVKD TKILAEETAA 900
  901 YEGNENEAEK EDALKSQIDD LPFYCIGFKS AAPEYTLRTR IWASLRSQTL YRTISGFMNY 960
  961 SRAIKLLYRV ENPEIVQMFG GNAEGLEREL EKMARRKFKF LVSMQRLAKF KPHELENAEF1020
 1021 LLRAYPDLQI AYLDEEPPLT EGEEPRIYSA LIDGHCEILD NGRRRPKFRV QLSGNPILGD1080
 1081 GKSDNQNHAL IFYRGEYIQL IDANQDNYLE ECLKIRSVLA EFEELNVEQV NPYAPGLRYE1140
 1141 EQTTNHPVAI VGAREYIFSE NSGVLGDVAA GKEQTFGTLF ARTLSQIGGK LHYGHPDFIN1200
 1201 ATFMTTRGGV SKAQKGLHLN EDIYAGMNAM LRGGRIKHCE YYQCGKGRDL GFGTILNFTT1260
 1261 KIGAGMGEQM LSREYYYLGT QLPVDRFLTF YYAHPGFHLN NLFIQLSLQM FMLTLVNLSS1320
 1321 LAHESIMCIY DRNKPKTDVL VPIGCYNFQP AVDWVRRYTL SIFIVFWIAF VPIVVQELIE1380
 1381 RGLWKATQRF FCHLLSLSPM FEVFAGQIYS SALLSDLAIG GARYISTGRG FATSRIPFSI1440
 1441 LYSRFAGSAI YMGARSMLML LFGTVAHWQA PLLWFWASLS SLIFAPFVFN PHQFAWEDFF1500
 1501 LDYRDYIRWL SRGNNQYHRN SWIGYVRMSR ARITGFKRKL VGDESEKAAG DASRAHRTNL1560
 1561 IMAEIIPCAI YAAGCFIAFT FINAQTGVKT TDDDRVNSVL RIIICTLAPI AVNLGVLFFC1620
 1621 MGMSCCSGPL FGMCCKKTGS VMAGIAHGVA VIVHIAFFIV MWVLESFNFV RMLIGVVTCI1680
 1681 QCQRLIFHCM TALMLTREFK NDHANTAFWT GKWYGKGMGY MAWTQPSREL TAKVIELSEF1740
 1741 AADFVLGHVI LICQLPLIII PKIDKFHSIM LFWLKPSRQI RPPIYSLKQT RLRKRMVKKY1800
 1801 CSLYFLVLAI FAGCIIGPAV ASAKIHKHIG DSLDGVVHNL FQPINTTNND TGSQMSTYQS1860
 1861 HYYTHTPSLK TWSTIK

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ANP1 BET5 FKS1 GEA2 MIR1 MNN10 PHB2 RPL17A RPL24A RPL28 RPS17B SEC7 TCB3 TRS23 YHR020W
View Details	(MIPS) Mewes HW, et al. (2004)		FEN1 FKS1 GSC2
View Details	Krogan NJ, et al. (2006)		CTP1 ERV25 FKS1 FTR1 MRH1 OLE1 PEX27 ROG3 RPL10 RPL36B RPL37A RPP1A SEC11 SPC1 SPC2 SPC3 TCM62 TSC13 YNR021W
View Details	Gavin AC, et al. (2002)		ADH1 ANP1 ARO1 ARX1 BET3 BET5 BMH1 BMH2 CCT4 CLU1 CRM1 CSE1 DHH1 DRS2 DUG1 ECM29 EMP24 ERO1 ERP1 ERP2 ERV25 ERV41 ERV46 FET5 FKS1 FLC2 FTH1 GCD6 GCN1 GCN20 GEA2 GFA1 GSG1 HAS1 HHF1, HHF2 HRP1 IDH2 KAP104 KAP123 KRE11 KTR3 LCB1 LCB2 LYS12 MIR1 MNN10 NAB2 NAT1 NEM1 NIP7 NUM1 OPI1 OSH2 PDR5 PHB1 PIL1 RGR1 RPN10 RPN3 RRP1 RVB2 SAM1 SAM2 SCS2 SEC13 SEC62 SEC63 SEC66 SEC7 SEC72 SKN1 SLC1 SRV2 STT4 SWH1 SWI3 TCB3 TRS120 TRS130 TRS20 TRS23 TRS31 TRS33 YAR044W YHR020W
View Details	Qiu et al. (2008)		FKS1 GSC2 RHO1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	TOR1	No Comments	Reinke A, et al. (2004)
View Run	TIM50	No Comments	Hazbun TR, et al. (2003)
View Run	TOR1	No Comments	Reinke A, et al. (2004)
View Run	TOR2	No Comments	Reinke A, et al. (2004)
View Run	NOP9	No Comments	Hazbun TR, et al. (2003)
View Run	TIM50	No Comments	Hazbun TR, et al. (2003)
View Run	MIA40	No Comments	Hazbun TR, et al. (2003)
View Run	LST8	No Comments	Reinke A, et al. (2004)
View Run	TOR2	No Comments	Reinke A, et al. (2004)
View Run	KOG1	No Comments	Reinke A, et al. (2004)
View Run	TOR2	No Comments	Reinke A, et al. (2004)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	RLF2	cac1: TAP-tagged	Green EM, et al (2005)
View Run	ASF1	3829 - low filter	Green EM, et al (2005)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	RLF2	phosphorylation data - cascade search	Green EM, et al (2005)
View Run	RLF2	identification data - first search in cascade	Green EM, et al (2005)
View Run	BOI1	Sample boi1 with gst from october 2005	McCusker D, et al (2007)
View Run	VAM3	sample: hx3	Hao Xu, et al. (2010)
View Run	VAM3	sample: hx4	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX11 - both fusion and trans-SNARE complex formation take place.	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX12 - trans-SNARE complex formation and fusion are inhibited (control).	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX13 - trans-SNARE complex forms but fusion is blocked.	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX14 - mixture in detergent (control).	Hao Xu, et al. (2010)
View Run	MLP2	#26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2	Keck JM, et al. (2011)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#10 Mitotic Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#02 Alpha Factor Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#01 Alpha factor Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#28 Asynchronous Prep5-TiO2 Phosphopeptide Enrichment	Keck JM, et al. (2011)
View Run	MLP2	#06 Alpha Factor Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#05 Alpha Factor Prep2-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#07 Alpha Factor Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#09 Alpha Factor Prep4-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	ILM1	FKS1	2	Lockshon D, et al. (2006)
View Screen	HMT1	FKS1	1	Unpublished Fields Lab Data
View Screen	UBP6	FKS1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	FKS1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..153]	MSA	108.145997	View MSA. No confident structure predictions are available.
2	View Details	[154..388]	MSA	1.05194	View MSA. Confident ab initio structure predictions are available.
3	View Details	[389..802]	deduced	N/A	No confident structure predictions are available.
4	View Details	[803..1666]	Pfam	1000.0	1,3-beta-glucan synthase component No confident structure predictions are available.
5	View Details	[1667..1876]	MSA	1.019943	View MSA. No confident structure predictions are available.
6	View Details	[620..784]	deduced	N/A	No confident structure predictions are available.
7	View Details	[785..850]	deduced	N/A	No confident structure predictions are available.
8	View Details	[851..941]	deduced	N/A	No confident structure predictions are available.
9	View Details	[942..1106]	MSA	2.048945	View MSA. No confident structure predictions are available.
10	View Details	[1107..1237]	MSA	3.048945	View MSA. No confident structure predictions are available.
11	View Details	[1238..1440]	MSA	2.050926	View MSA. No confident structure predictions are available.
12	View Details	[1441..1542]	MSA	1.046984	View MSA. No confident structure predictions are available.
13	View Details	[1543..1666]	deduced	N/A	No confident structure predictions are available.
14	View Details	[1667..1876]	MSA	2.023941	View MSA. No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

[View Details]

Source: Reynolds et al. 2008. Manuscript submitted

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2