Bait Protein: | VAM3 |
Organism: | Saccharomyces cerevisiae |
Publication: | Hao Xu , Youngsoo Jun, James Thompson, John Yates and William Wickner (2010) HOPS Prevents the Disassembly of Trans-SNARE Complexes by Sec17p/Sec18p during Membrane Fusion [In Press] |
Comments: | sample: hx3 |
Raw Data: |
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FILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
[ |
SEC17 | Peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' ste... | 15 | 26 | 56.2% | 0.0382 | 32803 |
[ |
NYV1 | v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent C... | 9 | 12 | 52.2% | 0.0204 | 28964 |
[ |
VAM7 | Component of the vacuole SNARE complex involved in vacuolar morphogenesis; SNAP-25 homolog; function... | 14 | 30 | 44.6% | 0.0408 | 36711 |
[ |
VAM3 | Syntaxin-related protein required for vacuolar assembly; functions with Vam7p in vacuolar protein tr... | 12 | 36 | 32.9% | 0.0546 | 32498 |
[ |
VTI1 | Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and ... | 8 | 20 | 32.3% | 0.0396 | 24668 |
[ |
RPP2B | Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction ... | 2 | 2 | 26.4% | 0.00781 | 11050 |
[ |
VMA6 | Subunit d of the five-subunit V0 integral membrane domain of vacuolar H+-ATPase (V-ATPase), an elect... | 4 | 6 | 21.4% | 0.00747 | 39791 |
[ |
PHO8 | Repressible alkaline phosphatase, a glycoprotein localized to the vacuole; regulated by levels of in... | 7 | 8 | 17.0% | 0.00607 | 63004 |
[ |
SEC18 | ATPase required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuol... | 6 | 7 | 13.6% | 0.00397 | 84056 |
[ |
KAR2 | ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... | 7 | 17 | 13.3% | 0.0107 | 74468 |
[ |
PDB1 | E1 beta subunit of the pyruvate dehydrogenase (PDH) complex, which is an evolutionarily-conserved mu... | 3 | 4 | 12.3% | 0.00469 | 40054 |
[ |
YKT6 | Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and wit... | 2 | 2 | 12.0% | 0.00429 | 22707 |
[ |
SHM2 | Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrof... | 4 | 8 | 11.7% | 0.00732 | 52219 |
[ |
IVY1 | Phospholipid-binding protein that interacts with both Ypt7p and Vps33p, may partially counteract the... | 3 | 8 | 11.7% | 0.00758 | 49976 |
[ |
VPH1 | Subunit a of vacuolar-ATPase V0 domain, one of two isoforms (Vph1p and Stv1p); Vph1p is located in V... | 7 | 11 | 11.5% | 0.00562 | 95529 |
[ |
YNL058C | Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to th... | 2 | 3 | 11.4% | 0.00408 | 35046 |
[ |
CDC19 | Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... | 3 | 3 | 10.6% | 0.00258 | 54545 |
[ |
VBA4 | Protein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GF... | 5 | 6 | 10.4% | 0.00335 | 85683 |
[ |
PHO84 | High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated... | 4 | 12 | 10.1% | 0.00878 | 64382 |
[ |
LAT1 | Dihydrolipoamide acetyltransferase component (E2) of pyruvate dehydrogenase complex, which catalyzes... | 3 | 4 | 9.8% | 0.00356 | 51818 |
[ |
YDR089W | Protein of unknown function; deletion confers resistance to Nickel | 5 | 10 | 9.3% | 0.00494 | 98712 |
[ |
PDI1 | Protein disulfide isomerase, multifunctional protein resident in the endoplasmic reticulum lumen, es... | 3 | 3 | 9.0% | 0.00247 | 58227 |
[ |
FKS1 | Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic sub... | 11 | 15 | 9.0% | 0.00343 | 214849 |
[ |
PDX1 | Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogena... | 2 | 2 | 9.0% | 0.00209 | 45362 |
[ |
YNL217W | Hypothetical protein | 2 | 2 | 8.9% | 0.00263 | 37151 |
[ |
PPN1 | Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer | 5 | 6 | 8.5% | 0.00382 | 78344 |
[ |
PRM5 | Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrit... | 2 | 3 | 7.9% | 0.00405 | 34651 |
[ |
NOP56 | Essential evolutionarily-conserved nucleolar protein component of the box C/D snoRNP complexes that ... | 2 | 2 | 7.7% | 0.0017 | 56864 |
[ |
FRA1 | Protein involved in negative regulation of transcription of iron regulon; forms an iron independent ... | 4 | 7 | 7.3% | 0.00401 | 84924 |
[ |
VAM6 | Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by fa... | 5 | 7 | 7.2% | 0.00287 | 122881 |
[ |
DAP2 | Dipeptidyl aminopeptidase, synthesized as a glycosylated precursor; localizes to the vacuolar membra... | 4 | 4 | 6.5% | 0.0021 | 93404 |
[ |
CCC1 | Putative vacuolar Fe2+/Mn2+ transporter; suppresses respiratory deficit of yfh1 mutants, which lack ... | 2 | 3 | 6.5% | 0.004 | 34250 |
[ |
ENO2 | Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... | 2 | 6 | 6.4% | 0.0059 | 46914 |
[ |
VPS33 | ATP-binding protein that is a subunit of the HOPS complex and of the CORVET tethering complex; essen... | 3 | 3 | 5.5% | 0.00186 | 79271 |
[ |
CPS1 | Vacuolar carboxypeptidase yscS; expression is induced under low-nitrogen conditions | 2 | 2 | 5.4% | 0.00149 | 64597 |
[ |
PEP3 | Component of CORVET tethering complex; vacuolar peripheral membrane protein that promotes vesicular ... | 4 | 4 | 5.3% | 0.00187 | 107398 |
[ |
YPK9 | Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-t... | 6 | 6 | 5.1% | 0.00175 | 166749 |
[ |
YCF1 | Vacuolar glutathione S-conjugate transporter of the ATP-binding cassette family, has a role in detox... | 5 | 8 | 4.8% | 0.00227 | 171120 |
[ |
TEF1, TEF2 | Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... | 2 | 3 | 4.1% | 0.00281 | 50033 |
[ |
LYP1 | Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of c... | 2 | 2 | 3.8% | 0.00141 | 68090 |
[ |
PHO91 | Low-affinity phosphate transporter of the vacuolar membrane; deletion of pho84, pho87, pho89, pho90,... | 3 | 3 | 3.6% | 0.00144 | 99490 |
[ |
FAA4 | Long chain fatty acyl-CoA synthetase, activates imported fatty acids with a preference for C12:0-C16... | 2 | 2 | 3.6% | 0.00124 | 77267 |
[ |
MNN1 | Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for add... | 2 | 2 | 3.5% | 0.00113 | 88529 |
[ |
VPS16 | Subunit of the vacuole fusion and protein sorting HOPS complex and the CORVET tethering complex; par... | 3 | 3 | 3.5% | 0.00161 | 92741 |
[ |
VPS41 | Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sort... | 3 | 4 | 3.5% | 0.00173 | 113412 |
[ |
PEP5 | Component of CORVET tethering complex; peripheral vacuolar membrane protein required for protein tra... | 3 | 3 | 3.0% | 0.00125 | 117477 |
[ |
VTC3 | Subunit of the vacuolar transporter chaperone (VTC) complex involved in membrane trafficking, vacuol... | 2 | 2 | 2.9% | 0.00103 | 96553 |
[ |
YLR241W | Putative protein of unknown function, may be involved in detoxification | 2 | 3 | 2.8% | 0.00165 | 89344 |
[ |
PHO90 | Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthet... | 2 | 2 | 2.7% | 9.75E-4 | 97689 |
[ |
PMC1 | Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activ... | 2 | 2 | 2.0% | 7.32E-4 | 130861 |
[ |
PEP1 | Type I transmembrane sorting receptor for multiple vacuolar hydrolases; cycles between the late-Golg... | 2 | 2 | 1.9% | 5.44E-4 | 177777 |
[ |
YBT1 | Transporter of the ATP-binding cassette (ABC) family involved in bile acid transport; similar to mam... | 2 | 4 | 1.8% | 0.00103 | 189161 |
[ |
MNR2 | Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has si... | 2 | 2 | 1.5% | 8.86E-4 | 109736 |