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Bait Protein: MLP2
Organism: Saccharomyces cerevisiae
Publication: Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561.
Comments: #09 Alpha Factor Prep4-TiO2 Flowthrough

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UNFILTERED Run Results: [filter]

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  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   MLP2 Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... 54 57 32.4% 0.179 195140
[View Peptides]   YIL002W-A Putative protein of unknown function; identified by expression profiling and mass spectrometry 1 1 30.4% 0.0765 7729
[View Peptides]   STM1 Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with r... 4 4 28.2% 0.0773 29995
[View Peptides]   RPS1A Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Bp and has similarit... 3 3 17.3% 0.0621 28743
[View Peptides]   RPS1B Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Ap and has similarit... 3 3 17.3% 0.0621 28812
[View Peptides]   SSB1 Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... 6 6 13.9% 0.0516 66602
[View Peptides]   RPL40B, RPL40A Fusion protein, identical to Rpl40Ap, that is cleaved to yield ubiquitin and a ribosomal protein of ... 1 1 12.5% 0.0412 14554
[View Peptides]   SSB2 Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... 5 5 11.7% 0.043 66595
[View Peptides]   PDC1 Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates... 3 3 11.0% 0.0281 61495
[View Peptides]   BBP1 Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periph... 2 2 10.6% 0.0274 45384
[View Peptides]   RPL13B Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl13Ap; not essential f... 1 1 10.6% 0.0265 22525
[View Peptides]   RPS31 Fusion protein that is cleaved to yield a ribosomal protein of the small (40S) subunit and ubiquitin... 1 1 10.5% 0.0347 17216
[View Peptides]   ENO2 Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... 3 4 9.4% 0.0483 46914
[View Peptides]   SPC110 Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... 6 6 9.0% 0.0335 111782
[View Peptides]   EFT2, EFT1 Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein s... 4 4 8.0% 0.0251 93289
[View Peptides]   SSA1 ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport;... 3 3 4.7% 0.0247 69768
[View Peptides]   UBI4 Ubiquitin, becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-... 1 1 4.2% 0.0138 42826
[View Peptides]   HSC82 Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, a... 2 2 4.1% 0.015 80900
[View Peptides]   PMA1 Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... 3 3 4.0% 0.0172 99619
[View Peptides]   Reverse_YHR174W ENO2 SGDID:S000001217, Chr VIII from 451327-452640, Verified ORF, "Enolase II, a phosphopyruvate hyd... 1 1 3.4% 0.012 46914
[View Peptides]   Reverse_YGR254W ENO1 SGDID:S000003486, Chr VII from 1000932-1002245, Verified ORF, "Enolase I, a phosphopyruvate hyd... 1 1 3.4% 0.012 46816
[View Peptides]   Reverse_YNL225C CNM67 SGDID:S000005169, Chr XIV from 224470-222725, reverse complement, Verified ORF, "Component of ... 1 1 2.9% 0.00906 67400
[View Peptides]   KAR1 Essential protein involved in karyogamy during mating and in spindle pole body duplication during mi... 1 1 2.8% 0.0122 50653
[View Peptides]   SUP35 Translation termination factor eRF3; altered protein conformation creates the [PSI(+)] prion, a domi... 1 1 2.5% 0.0077 76551
[View Peptides]   Reverse_YGL173C KEM1 SGDID:S000003141, Chr VII from 180119-175533, reverse complement, Verified ORF, "5'-3' exonucle... 2 2 2.3% 0.0069 175459
[View Peptides]   Reverse_YNL188W KAR1 SGDID:S000005132, Chr XIV from 286309-287610, Verified ORF, "Essential protein involved in kary... 1 1 1.8% 0.0122 50653
[View Peptides]   Reverse_YJR053W BFA1 SGDID:S000003814, Chr X from 533941-535665, Verified ORF, "Component of the GTPase-activating B... 1 1 1.7% 0.00917 66087
[View Peptides]   Reverse_YKR095W MLP1 SGDID:S000001803, Chr XI from 619447-625074, Verified ORF, "Myosin-like protein associated with... 1 1 1.3% 0.00281 218455
[View Peptides]   SPC97 Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the... 2 2 1.3% 0.0128 96825
[View Peptides]   Reverse_YHR172W SPC97 SGDID:S000001215, Chr VIII from 448335-450806, Verified ORF, "Component of the microtubule-nuc... 1 1 1.2% 0.0064 96825
[View Peptides]   POM152 Nuclear pore membrane glycoprotein; may be involved in duplication of nuclear pores and nuclear pore... 1 1 1.1% 0.00395 151652
[View Peptides]   Reverse_YLR342W FKS1 SGDID:S000004334, Chr XII from 809997-815627, Verified ORF, "Catalytic subunit of 1,3-beta-D-gl... 1 1 0.8% 0.00281 214849
[View Peptides]   FKS1 Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic sub... 1 1 0.4% 0.00281 214849

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