Bait Protein: | MLP2 |
Organism: | Saccharomyces cerevisiae |
Publication: | Keck JM, Jones MH, Wong CC, Binkley J, Chen D, Jaspersen SL, Holinger EP, Xu T, Niepel M, Rout MP, Vogel J, Sidow A, Yates JR, Winey M., A Cell Cycle Phosphoproteome of the Yeast Centrosome, Science 24 June 2011: 1557-1561. |
Comments: | #09 Alpha Factor Prep4-TiO2 Flowthrough |
Raw Data: |
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UNFILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
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MLP2 | Myosin-like protein associated with the nuclear envelope, connects the nuclear pore complex with the... | 54 | 57 | 32.4% | 0.179 | 195140 |
[ |
YIL002W-A | Putative protein of unknown function; identified by expression profiling and mass spectrometry | 1 | 1 | 30.4% | 0.0765 | 7729 |
[ |
STM1 | Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with r... | 4 | 4 | 28.2% | 0.0773 | 29995 |
[ |
RPS1A | Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Bp and has similarit... | 3 | 3 | 17.3% | 0.0621 | 28743 |
[ |
RPS1B | Ribosomal protein 10 (rp10) of the small (40S) subunit; nearly identical to Rps1Ap and has similarit... | 3 | 3 | 17.3% | 0.0621 | 28812 |
[ |
SSB1 | Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... | 6 | 6 | 13.9% | 0.0516 | 66602 |
[ |
RPL40B, RPL40A | Fusion protein, identical to Rpl40Ap, that is cleaved to yield ubiquitin and a ribosomal protein of ... | 1 | 1 | 12.5% | 0.0412 | 14554 |
[ |
SSB2 | Cytoplasmic ATPase that is a ribosome-associated molecular chaperone, functions with J-protein partn... | 5 | 5 | 11.7% | 0.043 | 66595 |
[ |
PDC1 | Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates... | 3 | 3 | 11.0% | 0.0281 | 61495 |
[ |
BBP1 | Protein required for the spindle pole body (SPB) duplication, localized at the central plaque periph... | 2 | 2 | 10.6% | 0.0274 | 45384 |
[ |
RPL13B | Protein component of the large (60S) ribosomal subunit, nearly identical to Rpl13Ap; not essential f... | 1 | 1 | 10.6% | 0.0265 | 22525 |
[ |
RPS31 | Fusion protein that is cleaved to yield a ribosomal protein of the small (40S) subunit and ubiquitin... | 1 | 1 | 10.5% | 0.0347 | 17216 |
[ |
ENO2 | Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... | 3 | 4 | 9.4% | 0.0483 | 46914 |
[ |
SPC110 | Inner plaque spindle pole body (SPB) component, ortholog of human kendrin; involved in connecting nu... | 6 | 6 | 9.0% | 0.0335 | 111782 |
[ |
EFT2, EFT1 | Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein s... | 4 | 4 | 8.0% | 0.0251 | 93289 |
[ |
SSA1 | ATPase involved in protein folding and nuclear localization signal (NLS)-directed nuclear transport;... | 3 | 3 | 4.7% | 0.0247 | 69768 |
[ |
UBI4 | Ubiquitin, becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-... | 1 | 1 | 4.2% | 0.0138 | 42826 |
[ |
HSC82 | Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, a... | 2 | 2 | 4.1% | 0.015 | 80900 |
[ |
PMA1 | Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plas... | 3 | 3 | 4.0% | 0.0172 | 99619 |
[ |
Reverse_YHR174W | ENO2 SGDID:S000001217, Chr VIII from 451327-452640, Verified ORF, "Enolase II, a phosphopyruvate hyd... | 1 | 1 | 3.4% | 0.012 | 46914 |
[ |
Reverse_YGR254W | ENO1 SGDID:S000003486, Chr VII from 1000932-1002245, Verified ORF, "Enolase I, a phosphopyruvate hyd... | 1 | 1 | 3.4% | 0.012 | 46816 |
[ |
Reverse_YNL225C | CNM67 SGDID:S000005169, Chr XIV from 224470-222725, reverse complement, Verified ORF, "Component of ... | 1 | 1 | 2.9% | 0.00906 | 67400 |
[ |
KAR1 | Essential protein involved in karyogamy during mating and in spindle pole body duplication during mi... | 1 | 1 | 2.8% | 0.0122 | 50653 |
[ |
SUP35 | Translation termination factor eRF3; altered protein conformation creates the [PSI(+)] prion, a domi... | 1 | 1 | 2.5% | 0.0077 | 76551 |
[ |
Reverse_YGL173C | KEM1 SGDID:S000003141, Chr VII from 180119-175533, reverse complement, Verified ORF, "5'-3' exonucle... | 2 | 2 | 2.3% | 0.0069 | 175459 |
[ |
Reverse_YNL188W | KAR1 SGDID:S000005132, Chr XIV from 286309-287610, Verified ORF, "Essential protein involved in kary... | 1 | 1 | 1.8% | 0.0122 | 50653 |
[ |
Reverse_YJR053W | BFA1 SGDID:S000003814, Chr X from 533941-535665, Verified ORF, "Component of the GTPase-activating B... | 1 | 1 | 1.7% | 0.00917 | 66087 |
[ |
Reverse_YKR095W | MLP1 SGDID:S000001803, Chr XI from 619447-625074, Verified ORF, "Myosin-like protein associated with... | 1 | 1 | 1.3% | 0.00281 | 218455 |
[ |
SPC97 | Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the... | 2 | 2 | 1.3% | 0.0128 | 96825 |
[ |
Reverse_YHR172W | SPC97 SGDID:S000001215, Chr VIII from 448335-450806, Verified ORF, "Component of the microtubule-nuc... | 1 | 1 | 1.2% | 0.0064 | 96825 |
[ |
POM152 | Nuclear pore membrane glycoprotein; may be involved in duplication of nuclear pores and nuclear pore... | 1 | 1 | 1.1% | 0.00395 | 151652 |
[ |
Reverse_YLR342W | FKS1 SGDID:S000004334, Chr XII from 809997-815627, Verified ORF, "Catalytic subunit of 1,3-beta-D-gl... | 1 | 1 | 0.8% | 0.00281 | 214849 |
[ |
FKS1 | Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic sub... | 1 | 1 | 0.4% | 0.00281 | 214849 |