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Protein Overview: SPB1

General Information:
Name(s) found: SPB1 / YCL054W [SGD]
Description(s) found:
Found 24 descriptions.
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  • AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 841 amino acids

    Gene Ontology:
    Cellular Component: nucleus [IDALink to PubMed article]
    nucleolus [IDALink to PubMed articleLink to PubMed article]
    Biological Process: rRNA methylation [IDALink to PubMed article][IGILink to PubMed articleLink to PubMed article][IMPLink to PubMed article]
    processing of 27S pre-rRNA [IDALink to PubMed article][IMPLink to PubMed articleLink to PubMed article]
    Molecular Function: rRNA (uridine-2'-O-)-methyltransferase activity [IGILink to PubMed article]
    rRNA (guanine) methyltransferase activity [IDALink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MGKTQKKNSK GRLDRYYYLA KEKGYRARSS FKIIQINEKY GHFLEKSKVV IDLCAAPGSW  60
   61 CQVASKLCPV NSLIIGVDIV PMKPMPNVIT FQSDITTEDC RSKLRGYMKT WKADTVLHDG 120
  121 APNVGLGWVQ DAFTQSQLTL QALKLAVENL VVNGTFVTKI FRSKDYNKLI WVFQQLFEKV 180
  181 EATKPPASRN VSAEIFVVCK GFKAPKRLDP RLLDPKEVFE ELPDGQQNME SKIYNPEKKV 240
  241 RKRQGYEEGD NLLYHETSIL DFVRTEDPIS MLGEMNKFTI DENDHEWKIL KKLKQTTDEF 300
  301 RSCIEDLKVL GKKDFKMILR WRKIAREILG IEVKDDAKTE IEVVPLTEEE QIEKDLQGLQ 360
  361 EKQRLNVKRE RRRKNEMKQK ELQRMQMNMI TPTDIGIEAA SLGKESLFNL KTAEKTGILN 420
  421 DLAKGKKRMI FTDDELAKDN DIYIDENIMI KDKDSAADAD DLESELNAMY SDYKTRRSER 480
  481 DAKFRAKQAR GGDNEEEWTG FNEGSLEKKE EEGKDYIEDN DDEGVEGDSD DDEAITNLIS 540
  541 KLKGQEGDHK LSSKARMIFN DPIFNNVEPD LPVNTVNDGI MSSESVGDIS KLNKKRKHEE 600
  601 MHQKQDEADS SDESSSDDSD FEIVANDNAS EEFDSDYDSE EEKNQTKKEK HSRDIDIATV 660
  661 EAMTLAHQLA LGQKNKHDLV DEGFNRYTFR DTENLPDWFL EDEKEHSKIN KPITKEAAMA 720
  721 IKEKIKAMNA RPIKKVAEAK ARKRMRAVAR LEKIKKKAGL INDDSDKTEK DKAEEISRLM 780
  781 RKVTKKPKTK PKVTLVVASG RNKGLAGRPK GVKGKYKMVD GVMKNEQRAL RRIAKKHHKK 840
  841 K

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) ARX1 BRX1 CIC1 DBP10 DRS1 ERB1 HAS1 MAK21 NIP7 NOG1 NOP15 NOP2 NOP7 NSA1 NUG1 RIX1 RIX7 RLP7 SPB1 TIF6 YTM1
    View Details Krogan NJ, et al. (2006) APN2 BFR1 BRX1 BUR2 LOC1 NHP6A PRK1 RIM1 SGV1 SPB1 TMA64 TSR1
    View Details Gavin AC, et al. (2002) ARX1 BEM2 BMS1 BRX1 BUD20 CIC1 CLU1 CYR1 CYS4 DBP10 DBP9 DCP2 DRS1 EBP2 ERB1 FPR4 GCN1 HAS1 HDA1 HER1 HIS3 INP2 IPI1 IPI3 KRE33 LHP1 LOC1 LSG1 MAK21 MAK5 MDN1 MIS1 MRT4 NAP1 NIP1 NIP7 NMD3 NOC2 NOC3 NOG1 NOG2 NOP1 NOP12 NOP15 NOP16 NOP2 NOP4 NOP56 NOP7 NSA1 NSA2 NUG1 PRT1 PUF6 REI1 RIX1 RIX7 RLP24 RLP7 RPA135 RPA190 RPF1 RPF2 RPG1 RPP2B RPT6 RRP1 RRP12 RSA3 RSA4 RTR1 SDA1 SEC7 SKI2 SNU13 SPB1 SPB4 SSF1 TIF6 TUB3 URB1 UTP10 UTP20 UTP22 YGL036W YTM1
    View Details Ho Y, et al. (2002) BMS1 CDC53 CFT1 CIC1 CKA2 CKB2 CLU1 COF1 CTF4 DBP10 DED81 DIA2 ENP1 ERB1 FIN1 FIP1 FPR3 FPR4 GLC7 GLC8 GPH1 HAS1 HER1 ILS1 KEL1 KRE33 KRI1 LST4 MAM33 MCM2 MCM3 MCM5 MHP1 NIP7 NMD3 NOP1 NOP12 NOP7 NSA1 NUG1 REG1 RPF2 RRP1 RRP12 RRP15 RRP17 RRP5 SCD5 SDS22 SEH1 SEN1 SKP1 SLT2 SPB1 SRP1 SSF1 SSF2 SSZ1 SUI2 SUI3 TIF6 URB1 UTP10 YAK1 YBL104C YTM1

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run MLP2 #24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #10 Mitotic Prep1-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #11 Mitotic Prep1-TiO2 enriched, new search criteria Keck JM, et al. (2011)
    View Run MLP2 #01 Alpha factor Prep1-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #28 Asynchronous Prep5-TiO2 Phosphopeptide Enrichment Keck JM, et al. (2011)
    View Run MLP2 #07 Alpha Factor Prep3-TiO2 Flowthrough Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    SHOWING SINGLE HITS. [ Hide Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

      BAIT PREY HITS PUBLICATION
    View Screen CDC48 SPB1 1 Unpublished Fields Lab Data

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

    No microscopy data found in the PDR for this protein.

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..204] PSI-BLAST 226.679424 RNA methyltransferase FtsJ
    2 View Details [205..532] PSI-BLAST 16.0 Heavy meromyosin subfragment
    3 View Details [533..629] PSI-BLAST 5.0 Tropomyosin
    4 View Details [630..713] PSI-BLAST 5.0 Tropomyosin
    5 View Details [714..841] MSA 8.703997 View MSA. No confident structure predictions are available.

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • rRNA (guanine) methyltransferase activity
    		•  4.21285117944777 bayes_pls_golite062009
  • methyltransferase activity
    		•  4.15445027778357 bayes_pls_golite062009
  • rRNA methyltransferase activity
    		•  4.09362182773099 bayes_pls_golite062009
  • N-methyltransferase activity
    		•  4.07806017452628 bayes_pls_golite062009
  • transferase activity, transferring one-carbon groups
    		•  4.07153733729662 bayes_pls_golite062009
  • protein methyltransferase activity
    		•  3.92746815303714 bayes_pls_golite062009
  • S-adenosylmethionine-dependent methyltransferase activity
    		•  3.39429041855776 bayes_pls_golite062009
  • RNA methyltransferase activity
    		•  3.07428840534971 bayes_pls_golite062009
  • histone methyltransferase activity
    		•  2.99461585647365 bayes_pls_golite062009
  • DNA-methyltransferase activity
    		•  2.43145108778901 bayes_pls_golite062009
  • protein-arginine N-methyltransferase activity
    		•  2.41876112831625 bayes_pls_golite062009
  • arginine N-methyltransferase activity
    		•  2.41876112831625 bayes_pls_golite062009
  • transferase activity
    		•  2.22303238506686 bayes_pls_golite062009
  • histone-arginine N-methyltransferase activity
    		•  2.04296107639729 bayes_pls_golite062009
  • RNA binding
    		•  1.99752836873461 bayes_pls_golite062009
  • rRNA (adenine) methyltransferase activity
    		•  1.83964463328968 bayes_pls_golite062009
  • tRNA methyltransferase activity
    		•  1.76571145896453 bayes_pls_golite062009
  • rRNA (adenine-N6,N6-)-dimethyltransferase activity
    		•  1.75675289134467 bayes_pls_golite062009
  • protein-arginine omega-N monomethyltransferase activity
    		•  1.66871163498737 bayes_pls_golite062009
  • catalytic activity
    		•  1.25333584501255 bayes_pls_golite062009
  • histone methyltransferase activity (H4-R3 specific)
    		•  1.1103522853299 bayes_pls_golite062009
  • transcription regulator activity
    		•  0.914549843668268 bayes_pls_golite062009
  • rRNA (uridine-2'-O-)-methyltransferase activity
    		•  0.90544396691236 bayes_pls_golite062009
  • protein-arginine omega-N asymmetric methyltransferase activity
    		•  0.89325812193225 bayes_pls_golite062009
  • DNA binding
    		•  0.86027591375666 bayes_pls_golite062009
  • rRNA (uridine) methyltransferase activity
    		•  0.742142092552002 bayes_pls_golite062009
  • site-specific DNA-methyltransferase (adenine-specific) activity
    		•  0.703178429468566 bayes_pls_golite062009
  • tRNA (cytosine-5-)-methyltransferase activity
    		•  0.672282175551951 bayes_pls_golite062009
  • tRNA (cytosine)-methyltransferase activity
    		•  0.672282175551951 bayes_pls_golite062009
  • nucleic acid binding
    		•  0.616949327277483 bayes_pls_golite062009
  • binding
    		•  0.383237930789959 bayes_pls_golite062009
  • O-methyltransferase activity
    		•  0.250946859465502 bayes_pls_golite062009
  • rRNA (guanine-N1-)-methyltransferase activity
    		•  0.234572322347698 bayes_pls_golite062009
  • tRNA (adenine-N1-)-methyltransferase activity
    		•  0.1772047818218 bayes_pls_golite062009
  • tRNA (adenine)-methyltransferase activity
    		•  0.1772047818218 bayes_pls_golite062009
  • mRNA binding
    		•  0.0540207721800283 bayes_pls_golite062009
  • snoRNA binding
    		•  0.00848192089639488 bayes_pls_golite062009
    2 No functions predicted.
    3 No functions predicted.
    4 No functions predicted.
    5 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.99

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle