Protein Overview: SRP1

General Information:
Name(s) found:	SRP1 / YNL189W [SGD]
Description(s) found: Found 30 descriptions. SHOW ALL	Karyopherin alpha homolog, forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; may also play a role in regulation of protein degradation [SGD]
Organism:	Saccharomyces cerevisiae
Length:	542 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA ] cytoplasm [IDA]
Biological Process:	nucleocytoplasmic transport [TAS]
Molecular Function:	protein transmembrane transporter activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MDNGTDSSTS KFVPEYRRTN FKNKGRFSAD ELRRRRDTQQ VELRKAKRDE ALAKRRNFIP  60
   61 PTDGADSDEE DESSVSADQQ FYSQLQQELP QMTQQLNSDD MQEQLSATVK FRQILSREHR 120
  121 PPIDVVIQAG VVPRLVEFMR ENQPEMLQLE AAWALTNIAS GTSAQTKVVV DADAVPLFIQ 180
  181 LLYTGSVEVK EQAIWALGNV AGDSTDYRDY VLQCNAMEPI LGLFNSNKPS LIRTATWTLS 240
  241 NLCRGKKPQP DWSVVSQALP TLAKLIYSMD TETLVDACWA ISYLSDGPQE AIQAVIDVRI 300
  301 PKRLVELLSH ESTLVQTPAL RAVGNIVTGN DLQTQVVINA GVLPALRLLL SSPKENIKKE 360
  361 ACWTISNITA GNTEQIQAVI DANLIPPLVK LLEVAEYKTK KEACWAISNA SSGGLQRPDI 420
  421 IRYLVSQGCI KPLCDLLEIA DNRIIEVTLD ALENILKMGE ADKEARGLNI NENADFIEKA 480
  481 GGMEKIFNCQ QNENDKIYEK AYKIIETYFG EEEDAVDETM APQNAGNTFG FGSNVNQQFN 540
  541 FN

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		CBC2 CSL4 DIS3 EAF3 EAF5 EAF7 KAP95 MED7 MTR3 NUP2 NUP60 RRP4 RRP40 RRP42 RRP43 RRP45 RRP46 RRP6 SKI6 SKI7 SRP1 STO1 SWC4 ULP1 YRA2
View Details	Krogan NJ, et al. (2006)		CBC2 KAP95 NUP1 NUP2 NUP60 OGG1 PCT1 RAD53 SRP1 STO1 ULP1 URB2 YRA2
View Details	Gavin AC, et al. (2006)		KAP95 NUP2 NUP60 SRP1
View Details	Gavin AC, et al. (2002)		ADA2 ADH1 AIR2 BLM10 BRR1 BUR2 CBC2 CDC55 CIN1 CMD1 COG3 CSE2 CSL4 CTR9 DAM1 DBP9 DIS3 ECM16 ECM29 ENO2 ERG13 EST3 FAB1 FBA1 GAL11 GCN1 GCN5 GFA1 GUS1 HHF1, HHF2 HOS2 HOS4 HTA1 IML1 INO4 KAP95 KEL1 LRP1 LTE1 LUC7 LYS12 MAM33 MDS3 MED1 MED11 MED2 MED4 MED6 MED7 MED8 MLC1 MLP1 MRPL6 MSH4 MTR3 MUD1 MYO2 MYO5 NAB3 NAB6 NAM8 NMD5 NPL3 NRD1 NUD1 NUP60 NUT1 NUT2 PDA1 PDB1 PFK1 PGD1 PGK1 PPH21 PPH22 PRE1 PRE10 PRE2 PRE3 PRE4 PRE5 PRE6 PRE7 PRE8 PRE9 PRP39 PRP40 PRP42 PSE1 PUP1 PUP2 PUP3 RGR1 ROX3 RPG1 RPO21 RPT3 RPT5 RRD2 RRP12 RRP4 RRP40 RRP42 RRP43 RRP45 RRP46 RRP6 RSE1 RTS1 RTS3 SAM1 SCL1 SCP160 SEN1 SET3 SGV1 SHE4 SIF2 SIN4 SKI2 SKI3 SKI6 SKI7 SLX9 SMD1 SMD2 SMD3 SNP1 SNT1 SNU56 SNU71 SPA2 SPT7 SRB2 SRB4 SRB5 SRB6 SRB7 SRB8 SRO9 SRP1 SSE2 SSN2 SSN3 SSN8 STO1 TAF6 TDH2 TDH3 TEF4 THO2 TIF1, TIF2 TIF4631 TIF4632 TPD3 ULP1 UTP10 UTP20 UTP22 YBL104C YFL006W YIL077C YIR035C YMR310C YOR1 YRA1 YRA2 ZDS1 ZDS2
View Details	Ho Y, et al. (2002)		AAC3 ACH1 ACO1 ADE5,7 ADH2 ADH4 AIR2 ARP2 ASF1 BLM10 BUD13 CBF5 CDC13 CDC5 CKA1 CKA2 CKB2 CLU1 COP1 COR1 CPA2 CYS4 DBP10 DED1 DIG2 DIS3 DOG1 DPB11 DSS1 EAF3 EDE1 ENP1 FAR1 FET4 FIP1 FPR3 GAL7 GND1 GSP1 HAS1 HEF3 HHF1, HHF2 HPR1 ILS1 IMD1 IMD4 IPP1 IRR1 IVY1 KAP95 KSS1 MAM33 MCD1 MCM4 MDH1 MES1 MFT1 MGE1 MRPS35 NAF1 NAM8 NMD3 NOP1 NOP12 NOP13 NOP56 NOP6 NOP7 NPL3 NUG1 NUP1 NUP2 NUP60 PAP1 PCT1 PDC5 PDS1 PFK2 PGM2 PIM1 PML1 PRP8 PTC2 PTC7 PUF6 QCR2 RAD53 REB1 RFC2 RLF2 RLI1 RNT1 RPA135 RPF2 RPT3 RRP1 RRP15 RRP4 RRP43 RRP5 RRP6 RTT103 SAN1 SAP185 SAP190 SEC53 SES1 SHQ1 SIF2 SIN3 SIR2 SIR3 SIR4 SIT4 SLD2 SMC1 SMC3 SMC4 SMC6 SNP1 SNU56 SPB1 SPC72 SPT2 SRP1 SSF1 SSK2 STE23 STO1 SUP45 SWI4 TAL1 TBF1 THO2 TIF4631 TIF6 TRA1 TRL1 UBI4 UBP15 UME1 UTP22 UTP7 VAC14 YFL006W YPL113C YPR090W YTA7 YTM1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	BOI1	Sample boi 1 with ha tag from october 2005	McCusker D, et al (2007)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	TWF1	SRP1	1	Unpublished Fields Lab Data
View Screen	MET18	SRP1	1	Unpublished Fields Lab Data
View Screen	YLR132C	SRP1	1	Hazbun TR, et al. (2003)
View Screen	MIG1	SRP1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	SRP1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..62]	PSI-BLAST	43.69897	STRUCTURE OF IMPORTIN BETA BOUND TO THE IBB DOMAIN OF IMPORTIN ALPHA
2	View Details	[63..205]	PSI-BLAST	10107.0	Karyopherin alpha
3	View Details	[206..372]	PSI-BLAST	10107.0	Karyopherin alpha
4	View Details	[373..542]	PSI-BLAST	10107.0	Karyopherin alpha

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
protein transporter activity	4.58709163830613	bayes_pls_golite062009
nuclear export signal receptor activity	3.48172991171337	bayes_pls_golite062009
binding	3.0667965351474	bayes_pls_golite062009
actin binding	2.8014924948341	bayes_pls_golite062009
nucleic acid binding	2.74745843355294	bayes_pls_golite062009
protein transmembrane transporter activity	2.74547353635661	bayes_pls_golite062009
cytoskeletal protein binding	2.61566088685247	bayes_pls_golite062009
transporter activity	2.48700774379895	bayes_pls_golite062009
substrate-specific transporter activity	2.26725422172281	bayes_pls_golite062009
transcription regulator activity	2.16673352512615	bayes_pls_golite062009
DNA binding	1.95242774903625	bayes_pls_golite062009
nuclear localization sequence binding	1.91487015967262	bayes_pls_golite062009
protein binding	1.73688475473996	bayes_pls_golite062009
molecular transducer activity	1.32874350341969	bayes_pls_golite062009
signal transducer activity	1.32874350341969	bayes_pls_golite062009
transmembrane transporter activity	1.28481004323799	bayes_pls_golite062009
transcription factor activity	1.20660383756339	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	1.09652988954158	bayes_pls_golite062009
signal sequence binding	0.57106697498903	bayes_pls_golite062009
RNA binding	0.40024158562931	bayes_pls_golite062009
structural molecule activity	0.279926218252647	bayes_pls_golite062009
importin-alpha export receptor activity	0.259676026705859	bayes_pls_golite062009
active transmembrane transporter activity	0.252495966234234	bayes_pls_golite062009
ion transmembrane transporter activity	0.248585912061178	bayes_pls_golite062009
primary active transmembrane transporter activity	0.226380819487924	bayes_pls_golite062009
P-P-bond-hydrolysis-driven transmembrane transporter activity	0.217517113570989	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances	0.178770060806735	bayes_pls_golite062009
ATPase activity, coupled to movement of substances	0.175241020572885	bayes_pls_golite062009
ATPase activity, coupled to transmembrane movement of substances	0.174922130116569	bayes_pls_golite062009
transcription activator activity	0.160275383613705	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.100181463251306	bayes_pls_golite062009
pyrophosphatase activity	0.0635404099346928	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	0.0548082069333885	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	0.052195223119721	bayes_pls_golite062009
cation transmembrane transporter activity	0.0405268469927988	bayes_pls_golite062009
transcription factor binding	0.0221966060394205	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2