Protein Overview: KAP95

General Information:
Name(s) found:	KAP95 / YLR347C [SGD]
Description(s) found: Found 32 descriptions. SHOW ALL	Karyopherin beta, forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p [SGD]
Organism:	Saccharomyces cerevisiae
Length:	861 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA] nuclear pore [IDA]
Biological Process:	protein import into nucleus [TAS] nuclear pore complex assembly [IMP]
Molecular Function:	protein transmembrane transporter activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSTAEFAQLL ENSILSPDQN IRLTSETQLK KLSNDNFLQF AGLSSQVLID ENTKLEGRIL  60
   61 AALTLKNELV SKDSVKTQQF AQRWITQVSP EAKNQIKTNA LTALVSIEPR IANAAAQLIA 120
  121 AIADIELPHG AWPELMKIMV DNTGAEQPEN VKRASLLALG YMCESADPQS QALVSSSNNI 180
  181 LIAIVQGAQS TETSKAVRLA ALNALADSLI FIKNNMEREG ERNYLMQVVC EATQAEDIEV 240
  241 QAAAFGCLCK IMSLYYTFMK PYMEQALYAL TIATMKSPND KVASMTVEFW STICEEEIDI 300
  301 AYELAQFPQS PLQSYNFALS SIKDVVPNLL NLLTRQNEDP EDDDWNVSMS AGACLQLFAQ 360
  361 NCGNHILEPV LEFVEQNITA DNWRNREAAV MAFGSIMDGP DKVQRTYYVH QALPSILNLM 420
  421 NDQSLQVKET TAWCIGRIAD SVAESIDPQQ HLPGVVQACL IGLQDHPKVA TNCSWTIINL 480
  481 VEQLAEATPS PIYNFYPALV DGLIGAANRI DNEFNARASA FSALTTMVEY ATDTVAETSA 540
  541 SISTFVMDKL GQTMSVDENQ LTLEDAQSLQ ELQSNILTVL AAVIRKSPSS VEPVADMLMG 600
  601 LFFRLLEKKD SAFIEDDVFY AISALAASLG KGFEKYLETF SPYLLKALNQ VDSPVSITAV 660
  661 GFIADISNSL EEDFRRYSDA MMNVLAQMIS NPNARRELKP AVLSVFGDIA SNIGADFIPY 720
  721 LNDIMALCVA AQNTKPENGT LEALDYQIKV LEAVLDAYVG IVAGLHDKPE ALFPYVGTIF 780
  781 QFIAQVAEDP QLYSEDATSR AAVGLIGDIA AMFPDGSIKQ FYGQDWVIDY IKRTRSGQLF 840
  841 SQATKDTARW AREQQKRQLS L

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		CBC2 KAP95 NAB3 NUP1 NUP2 NUP60 SRP1 STO1 ULP1
View Details	Krogan NJ, et al. (2006)		CBC2 KAP95 NUP1 NUP2 NUP60 OGG1 PCT1 RAD53 SRP1 STO1 ULP1 URB2 YRA2
View Details	Gavin AC, et al. (2006)		KAP95 NUP2 NUP60 SRP1
View Details	Gavin AC, et al. (2002)		AIR2 BLM10 BRR1 BUR2 CBC2 CCL1 CDC55 CIN1 CMR1 CPR1 DAM1 DBP9 DCS2 DNA2 ECM29 ERG13 EST3 FAP1 FPR1 GFA1 GUS1 HDA1 HDA2 HDA3 HHF1, HHF2 HOS2 HOS4 HSC82 HTA1 IML1 INO4 KAP95 KEL1 KIN28 LCD1 LTE1 LUC7 LYS12 MDS3 MEC1 MGM101 MLH1 MPH1 MRPS28 MSH2 MSH3 MSH4 MSH6 MUD1 MYO5 NAB3 NAB6 NAM8 NPL3 NRD1 NUD1 NUP60 OLA1 PDA1 PDB1 PFK1 PGK1 PMS1 PPH21 PPH22 PRE1 PRE10 PRE2 PRE3 PRE4 PRE5 PRE6 PRE7 PRE8 PRE9 PRO2 PRP39 PRP40 PRP42 PSE1 PUP1 PUP2 PUP3 RAD16 RAD23 RAD34 RAD4 RAD52 RFA1 RFA2 RFA3 RGR1 RIM1 RNH1 RPN10 RPN12 RPN3 RPN6 RPN8 RPN9 RPT2 RPT3 RPT5 RPT6 RRD2 RRP12 RSC8 RSE1 RTS1 RTS3 RVB2 SCL1 SCP160 SEN1 SET3 SGS1 SGV1 SHS1 SIF2 SLX9 SMD1 SMD2 SMD3 SNF2 SNP1 SNT1 SNU56 SNU71 SPA2 SPT7 SRO7 SRO9 SRP1 SSL1 STO1 TDH2 TDH3 TEF4 TFA1 TFB1 TFB3 TFB4 THO2 TIF1, TIF2 TIF4631 TIF4632 TIF6 TOP3 TPD3 ULP1 VAS1 VPS1 YBL104C YFL006W YHR122W YMR310C YOR1 YRA1 YRA2 ZDS1 ZDS2
View Details	Ho Y, et al. (2002)		AIM46 ASF1 CBF5 CBP2 CDC13 CDC5 DBP7 DIS3 DRS1 EAF3 EDE1 ENP2 ERB1 FIP1 GCN1 GSP1 GSP2 HAS1 HPR1 HTB1 HXT7 IRR1 ISW1 ISW2 IVY1 KAP95 KRE33 KRI1 KRR1 LEM3 MCD1 MES1 MFT1 MNP1 MOG1 MSS116 NAM8 NMD5 NOP13 NOP2 NOP58 NOP6 NOP7 NUG1 NUP1 NUP2 NUP60 PAP1 PCT1 PTC2 PUF6 PWP1 RAD53 REB1 RLF2 RNT1 RPF2 RRP4 RRP43 RRP6 RTT103 SIF2 SIN3 SMC1 SMC3 SNU56 SPC72 SRP1 STO1 SWI4 TBF1 TCP1 THO2 TIF1, TIF2 TIF4631 TIF6 TRA1 TSR1 UME1 UTP22 YLR326W YPR090W YTA7
View Details	Qiu et al. (2008)		KAP122 KAP123 KAP95

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	NOP9	No Comments	Hazbun TR, et al. (2003)
View Run	GWT1	No Comments	Hazbun TR, et al. (2003)
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	BOI1	Sample boi 1 with ha tag from october 2005	McCusker D, et al (2007)
View Run	MLP2	#25 Asynchronous Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2	Keck JM, et al. (2011)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	KAP95	Huh WK, et al. (2003)

Searching the YRC Public Image Repository, found results:

Currently showing to of results.

[View Search in the YRC Public Image Repository]

Searching the YRC Public Image Repository, found no results.

Ginzu Version Information

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

Gene Ontology predictions

Term	Confidence	Notes
protein transporter activity	4.58709163830613	bayes_pls_golite062009
binding	3.0667965351474	bayes_pls_golite062009
protein transmembrane transporter activity	2.74547353635661	bayes_pls_golite062009
transporter activity	2.48700774379895	bayes_pls_golite062009
nucleic acid binding	2.34284977509828	bayes_pls_golite062009
substrate-specific transporter activity	2.26725422172281	bayes_pls_golite062009
nuclear export signal receptor activity	2.13470398426489	bayes_pls_golite062009
nuclear localization sequence binding	1.91487015967262	bayes_pls_golite062009
transcription regulator activity	1.76231475025891	bayes_pls_golite062009
protein binding	1.73688475473996	bayes_pls_golite062009
DNA binding	1.62032607987786	bayes_pls_golite062009
transmembrane transporter activity	1.28481004323799	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	1.09652988954158	bayes_pls_golite062009
transcription factor activity	0.923253759652909	bayes_pls_golite062009
structural molecule activity	0.908029258648777	bayes_pls_golite062009
translation initiation factor activity	0.83079633551839	bayes_pls_golite062009
translation regulator activity	0.82193201542158	bayes_pls_golite062009
translation factor activity, nucleic acid binding	0.803700928110921	bayes_pls_golite062009
cytoskeletal protein binding	0.773975545005505	bayes_pls_golite062009
signal transducer activity	0.662662274021266	bayes_pls_golite062009
molecular transducer activity	0.662662274021266	bayes_pls_golite062009
actin binding	0.572473732371314	bayes_pls_golite062009
signal sequence binding	0.57106697498903	bayes_pls_golite062009
mRNA binding	0.46864854653143	bayes_pls_golite062009
RNA binding	0.40024158562931	bayes_pls_golite062009
active transmembrane transporter activity	0.252495966234234	bayes_pls_golite062009
ion transmembrane transporter activity	0.248585912061178	bayes_pls_golite062009
primary active transmembrane transporter activity	0.226380819487924	bayes_pls_golite062009
P-P-bond-hydrolysis-driven transmembrane transporter activity	0.217517113570989	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, catalyzing transmembrane movement of substances	0.178770060806735	bayes_pls_golite062009
ATPase activity, coupled to movement of substances	0.175241020572885	bayes_pls_golite062009
ATPase activity, coupled to transmembrane movement of substances	0.174922130116569	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.100181463251306	bayes_pls_golite062009
pyrophosphatase activity	0.0635404099346928	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	0.0548082069333885	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	0.052195223119721	bayes_pls_golite062009
cation transmembrane transporter activity	0.0405268469927988	bayes_pls_golite062009
transcription activator activity	0.0232727461674547	bayes_pls_golite062009

No functions predicted.

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..248]	PSI-BLAST	1397.0	Importin beta
2	View Details	[249..442]	PSI-BLAST	1397.0	Importin beta
3	View Details	[443..541]	PSI-BLAST	1397.0	Importin beta
4	View Details	[542..648]	PSI-BLAST	1397.0	Importin beta
5	View Details	[649..861]	PSI-BLAST	1397.0	Importin beta

Protein Overview: KAP95

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: