Protein Overview: SCP160

General Information:
Name(s) found:	SCP160 / YJL080C [SGD]
Description(s) found: Found 31 descriptions. SHOW ALL	Essential RNA-binding G protein effector of mating response pathway, mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins [SGD]
Organism:	Saccharomyces cerevisiae
Length:	1222 amino acids
Gene Ontology:
Cellular Component:	endoplasmic reticulum membrane [IDA] polysome [IDA] nuclear membrane-endoplasmic reticulum network [IDA]
Biological Process:	meiotic telomere clustering [IMP] intracellular mRNA localization [IPI] response to drug [IMP] chromosome segregation [IMP] chromatin silencing at silent mating-type cassette [IMP] chromatin silencing at telomere [IGI][IMP] pheromone-dependent signal transduction involved in conjugation with cellular fusion [IGI]
Molecular Function:	RNA binding [IDA] G-protein alpha-subunit binding [IPI]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSEEQTAIDS PPSTVEGSVE TVTTIDSPST TASTIAATAE EHPQLEKKPT PLPSLKDLPS  60
   61 LGSNAAFANV KVSWGPNMKP AVSNSPSPSP SAPSLTTGLG AKRMRSKNIQ EAFTLDLQSQ 120
  121 LSITKPELSR IVQSVKKNHD VSVESTLSKN ARTFLVSGVA ANVHEAKREL VKKLTKPINA 180
  181 VIEVPSKCKA SIIGSGGRTI REISDAYEVK INVSKEVNEN SYDEDMDDTT SNVSLFGDFE 240
  241 SVNLAKAKIL AIVKEETKNA TIKLVVEDEK YLPYIDVSEF ASDEGDEEVK VQFYKKSGDI 300
  301 VILGPREKAK ATKTSIQDYL KKLASNLDEE KVKIPSKFQF LIDAEELKEK YNVIVTFPST 360
  361 PDDELVSFVG LRDKVGEAIT YARSSSKSYV VESLDISKAH SKNLTHAKNL IMYFTKYSVL 420
  421 KGLEESHPNV KISLPSIQSL PTAETVTIHI SAKSDEANDI KAVRKELISF VNNIPPSETL 480
  481 VITDLDYELF GGSIKHCLLA SESSVAFVQF GDYYPNDNSI LLVALTEDED FKPSIEEIQA 540
  541 SLNKANESLN SLRTKQNNME TKTYEFSEEV QDSLFKPSSA TWKLIMEDIS EQEGHLQIKL 600
  601 HTPEENQLTV RGDEKAAKAA NKIFESILNS PSSKSKMTVN IPANSVARLI GNKGSNLQQI 660
  661 REKFACQIDI PNEENNNASK DKTVEVTLTG LEYNLTHAKK YLAAEAKKWA DIITKELIVP 720
  721 VKFHGSLIGP HGTYRNRLQE KYNVFINFPR DNEIVTIRGP SRGVNKAHEE LKALLDFEME 780
  781 NGHKMVINVP AEHVPRIIGK NGDNINDIRA EYGVEMDFLQ KSTDPKAQET GEVELEITGS 840
  841 RQNIKDAAKR VESIVAEASD FVTEVLKIDH KYHKSIVGSG GHILREIISK AGGEEIRNKS 900
  901 VDIPNADSEN KDITVQGPQK FVKKVVEEIN KIVKDAENSV TKTIDIPAER KGALIGPGGI 960
  961 VRRQLESEFN INLFVPNKDD PSGKITITGA PENVEKAEKK ILNEIIRENF DREVDVPASI1020
 1021 YEYVSERGAF IQKLRMDLSV NVRFGNTSKK ANKLARAPIE IPLEKVCGST EGENAEKTKF1080
 1081 TIEEVGAPTS SEEGDITMRL TYEPIDLSSI LSDGEEKEVT KDTSNDSAKK EEALDTAVKL1140
 1141 IKERIAKAPS ATYAGYVWGA DTRRFNMIVG PGGSNIKKIR EAADVIINVP RKSDKVNDVV1200
 1201 YIRGTKAGVE KAGEMVLKSL RR

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		SCP160 SSZ1
View Details	Krogan NJ, et al. (2006)		ALA1 ARO1 DUG1 GDH1 HIS4 HIS6 HRD1 MET6 OYE2 RAD14 SCP160 SPE3 SPE4 TIM11 URA6 YLF2 YRB2
View Details	Gavin AC, et al. (2006)		SCP160 SSZ1 ZUO1
View Details	Gavin AC, et al. (2002)		AIR2 AMN1 ASC1 BEM2 BRR1 BUD14 BUR2 CBC2 CKA1 GCD6 HTA1 KAP95 KIN3 LUC7 MIS1 MSH4 MUD1 NAB3 NAB6 NAM8 NPL3 NRD1 NUP60 PRP39 PRP40 PRP42 RSE1 SCP160 SEN1 SGV1 SKI3 SMD1 SMD2 SMD3 SNP1 SNU56 SNU71 SRO9 SRP1 SSZ1 STO1 THO2 TIF4631 TIF4632 YRA2 ZUO1
View Details	Ho Y, et al. (2002)		AAT2 ACH1 ACO1 ACS2 ADE3 ADE6 ADO1 AFR1 AHP1 ALA1 APE2 ARA1 ARC1 ARF1 ATP3 BAT1 CAR2 CDC10 CDC33 CLU1 COF1 COR1 CPR1 CYR1 CYS3 CYS4 DED81 DPS1 EGD1 ERG10 ERG13 ERG20 ERG6 FPR1 FRS2 GCY1 GND1 GND2 GPH1 GRS1 GRX1 GUS1 HEF3 HHF1, HHF2 HOM2 HTB2 HYP2 ILS1 IMD3 KRS1 LRO1 LSP1 MDH3 MET6 MKT1 NTF2 OLA1 OYE2 PAB1 PFK1 PFK2 PGM2 PIL1 PMI40 PRM2 PRP6 RNR2 RSN1 SAC6 SAH1 SCP160 SEC53 SEN15 SES1 SNU13 SOD1 SSZ1 TEF4 THR4 THS1 TIF1, TIF2 TMA19 TRR1 TSA2 UBA1 VMA4 VMA5 WTM1 YDL124W YGR067C YNK1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	MLP2	#19 Asynchronous Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#14 Mitotic Prep2-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#19b Asynchronous Prep1-new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#19a Asynchronous Prep1-new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	GPA1	SCP160	2	Unpublished Fields Lab Data
View Screen	SCP160	TUP1	2	Unpublished Fields Lab Data
View Screen	SCP160	CDC26	2	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	SCP160	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..171]	MSA	1.045986	View MSA. No confident structure predictions are available.
2	View Details	[172..266]	PSI-BLAST	7.522879	RNA splicing factor 1
3	View Details	[267..339]	deduced	N/A	Confident ab initio structure predictions are available.
4	View Details	[340..417]	PSI-BLAST	3.221849	S1 domain of NusA; Transcription factor NusA, C-terminal domains; Transcription factor NusA, N-terminal domain
5	View Details	[418..483]	PSI-BLAST	44.39794	Polynucleotide phosphorylase/guanosine pentaphosphate synthase (PNPase/GPSI), domain 3; S1 RNA-binding domain of polyribonucleotide phosphorylase, PNPase; Polynucleotide phosphorylase/guanosine pentaphosphate synthase (PNPase/GPSI), domains 1 and 4; Polynucleotide phosphorylase/guanosine pentaphosphate synthase (PNPase/GPSI), domain 6; Polynucleotide phosphorylase/guanosine pentaphosphate synthase (PNPase/GPSI), domains 2 and 5
6	View Details	[484..508] [643..701]	PSI-BLAST	44.39794	Polynucleotide phosphorylase/guanosine pentaphosphate synthase (PNPase/GPSI), domain 3; S1 RNA-binding domain of polyribonucleotide phosphorylase, PNPase; Polynucleotide phosphorylase/guanosine pentaphosphate synthase (PNPase/GPSI), domains 1 and 4; Polynucleotide phosphorylase/guanosine pentaphosphate synthase (PNPase/GPSI), domain 6; Polynucleotide phosphorylase/guanosine pentaphosphate synthase (PNPase/GPSI), domains 2 and 5
7	View Details	[509..642] [702..849]	PSI-BLAST	44.39794	Polynucleotide phosphorylase/guanosine pentaphosphate synthase (PNPase/GPSI), domain 3; S1 RNA-binding domain of polyribonucleotide phosphorylase, PNPase; Polynucleotide phosphorylase/guanosine pentaphosphate synthase (PNPase/GPSI), domains 1 and 4; Polynucleotide phosphorylase/guanosine pentaphosphate synthase (PNPase/GPSI), domain 6; Polynucleotide phosphorylase/guanosine pentaphosphate synthase (PNPase/GPSI), domains 2 and 5
8	View Details	[850..941]	ORFEUS	11.56	HnRNP K, KH3
9	View Details	[942..1010]	PSI-BLAST	32.383689	Vigilin, KH6
10	View Details	[1011..1082]	PSI-BLAST	3.39794	S1 RNA-binding domain of polyribonucleotide phosphorylase, PNPase
11	View Details	[1083..1148]	PSI-BLAST	18.045757	Far upstream binding element, FBP, KH3 and KH4 domains
12	View Details	[1149..1222]	PSI-BLAST	18.045757	Far upstream binding element, FBP, KH3 and KH4 domains

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
RNA binding	4.12546383654586	bayes_pls_golite062009
nucleic acid binding	3.94829960615623	bayes_pls_golite062009
binding	2.88506305420422	bayes_pls_golite062009
mRNA binding	2.56780637687327	bayes_pls_golite062009
transcription regulator activity	1.92947291634926	bayes_pls_golite062009
DNA binding	1.60641898958006	bayes_pls_golite062009
transcription repressor activity	1.48649836219447	bayes_pls_golite062009
protein binding	1.09698608157472	bayes_pls_golite062009
signal transducer activity	1.00592302262807	bayes_pls_golite062009
molecular transducer activity	1.00592302262807	bayes_pls_golite062009
transcription factor activity	0.693474044974507	bayes_pls_golite062009
catalytic activity	0.1435046019283	bayes_pls_golite062009

Term	Confidence	Notes
RNA binding	4.53756130039297	bayes_pls_golite062009
nucleic acid binding	4.20349765519502	bayes_pls_golite062009
binding	3.03029468866374	bayes_pls_golite062009
mRNA binding	2.96669269799942	bayes_pls_golite062009
transcription regulator activity	1.94297875367222	bayes_pls_golite062009
DNA binding	1.62328996594128	bayes_pls_golite062009
transcription repressor activity	1.56066397370123	bayes_pls_golite062009
protein binding	1.16541072642796	bayes_pls_golite062009
signal transducer activity	1.00684766673868	bayes_pls_golite062009
molecular transducer activity	1.00684766673868	bayes_pls_golite062009
transcription factor activity	0.687475481524107	bayes_pls_golite062009
catalytic activity	0.113112541746059	bayes_pls_golite062009
	0.0350881281129536	bayes_pls_golite062009

No functions predicted.

Term	Confidence	Notes
structural constituent of ribosome	3.83603737358696	bayes_pls_golite062009
transcription regulator activity	3.74935939787001	bayes_pls_golite062009
structural molecule activity	3.27216171079692	bayes_pls_golite062009
nucleic acid binding	1.97035850468348	bayes_pls_golite062009
RNA binding	1.82714562401326	bayes_pls_golite062009
binding	1.5466198129438	bayes_pls_golite062009
transcription termination factor activity	1.53584399356788	bayes_pls_golite062009
DNA binding	1.42930679560452	bayes_pls_golite062009
transcription repressor activity	0.805620048597073	bayes_pls_golite062009
ribonuclease activity	0.621577761289592	bayes_pls_golite062009
transcription factor activity	0.599956044396141	bayes_pls_golite062009
telomeric DNA binding	0.55247451742813	bayes_pls_golite062009
protein binding	0.523813050066193	bayes_pls_golite062009
deacetylase activity	0.446316761887039	bayes_pls_golite062009
ATPase activity	0.368110506118156	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.31569932728929	bayes_pls_golite062009
3'-5'-exoribonuclease activity	0.2703049141847	bayes_pls_golite062009
pyrophosphatase activity	0.256048137250105	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	0.237784820115582	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	0.235837031080758	bayes_pls_golite062009
exoribonuclease activity, producing 5'-phosphomonoesters	0.20814642097724	bayes_pls_golite062009
translation initiation factor activity	0.2080607201312	bayes_pls_golite062009
exoribonuclease activity	0.19299160134668	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.158428921465478	bayes_pls_golite062009
catalytic activity	0.10089766806364	bayes_pls_golite062009
ATPase activity, coupled	0.0992081031610161	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	0.0481402103729352	bayes_pls_golite062009

Term	Confidence	Notes
RNA binding	4.12546383654586	bayes_pls_golite062009
nucleic acid binding	3.94829960615623	bayes_pls_golite062009
binding	2.88506305420422	bayes_pls_golite062009
mRNA binding	2.56780637687327	bayes_pls_golite062009
transcription regulator activity	1.92947291634926	bayes_pls_golite062009
DNA binding	1.60641898958006	bayes_pls_golite062009
transcription repressor activity	1.48649836219447	bayes_pls_golite062009
protein binding	1.09698608157472	bayes_pls_golite062009
signal transducer activity	1.00592302262807	bayes_pls_golite062009
molecular transducer activity	1.00592302262807	bayes_pls_golite062009
transcription factor activity	0.693474044974507	bayes_pls_golite062009
catalytic activity	0.1435046019283	bayes_pls_golite062009

Term	Confidence	Notes
RNA binding	4.53756130039297	bayes_pls_golite062009
nucleic acid binding	4.20349765519502	bayes_pls_golite062009
binding	3.03029468866374	bayes_pls_golite062009
mRNA binding	2.96669269799942	bayes_pls_golite062009
transcription regulator activity	1.94297875367222	bayes_pls_golite062009
DNA binding	1.62328996594128	bayes_pls_golite062009
transcription repressor activity	1.56066397370123	bayes_pls_golite062009
protein binding	1.16541072642796	bayes_pls_golite062009
signal transducer activity	1.00684766673868	bayes_pls_golite062009
molecular transducer activity	1.00684766673868	bayes_pls_golite062009
transcription factor activity	0.687475481524107	bayes_pls_golite062009
catalytic activity	0.113112541746059	bayes_pls_golite062009
	0.0350881281129536	bayes_pls_golite062009

Term	Confidence	Notes
structural constituent of ribosome	3.72252697400364	bayes_pls_golite062009
nucleic acid binding	3.7132569326175	bayes_pls_golite062009
structural molecule activity	3.13468682204566	bayes_pls_golite062009
DNA binding	3.12689559879854	bayes_pls_golite062009
binding	2.40532042551671	bayes_pls_golite062009
RNA binding	1.76502371106766	bayes_pls_golite062009
transcription regulator activity	1.50283169429524	bayes_pls_golite062009
structure-specific DNA binding	1.10289736160471	bayes_pls_golite062009
single-stranded DNA binding	0.96886436248958	bayes_pls_golite062009
transcription repressor activity	0.805620048597073	bayes_pls_golite062009
transcription factor activity	0.599956044396141	bayes_pls_golite062009
telomeric DNA binding	0.55247451742813	bayes_pls_golite062009
ribonuclease activity	0.498439111185072	bayes_pls_golite062009
sequence-specific DNA binding	0.3506031439435	bayes_pls_golite062009
translation regulator activity	0.31332179848255	bayes_pls_golite062009
translation factor activity, nucleic acid binding	0.263197317040851	bayes_pls_golite062009
translation initiation factor activity	0.2080607201312	bayes_pls_golite062009
nuclease activity	0.17660139576327	bayes_pls_golite062009
protein binding	0.152645195799531	bayes_pls_golite062009

Term	Confidence	Notes
RNA binding	4.53756130039297	bayes_pls_golite062009
nucleic acid binding	4.20349765519502	bayes_pls_golite062009
binding	3.03029468866374	bayes_pls_golite062009
mRNA binding	2.96669269799942	bayes_pls_golite062009
transcription regulator activity	1.94297875367222	bayes_pls_golite062009
DNA binding	1.62328996594128	bayes_pls_golite062009
transcription repressor activity	1.56066397370123	bayes_pls_golite062009
protein binding	1.16541072642796	bayes_pls_golite062009
signal transducer activity	1.00684766673868	bayes_pls_golite062009
molecular transducer activity	1.00684766673868	bayes_pls_golite062009
transcription factor activity	0.687475481524107	bayes_pls_golite062009
catalytic activity	0.113112541746059	bayes_pls_golite062009
	0.0350881281129536	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2