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Protein Overview: MKT1

General Information:
Name(s) found: MKT1 / YNL085W [SGD]
Description(s) found:
Found 30 descriptions.
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  • Protein that forms a complex with Pbp1p that may mediate posttranscriptional regulation of HO endonuclease; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 830 amino acids

    Gene Ontology:
    Cellular Component: polysome [IDALink to PubMed article]
    cytoplasm [IDALink to PubMed article]
    Biological Process: interspecies interaction between organisms [IMPLink to PubMed article]
    positive regulation of translation [IMPLink to PubMed article][IPILink to PubMed article]
    Molecular Function: nuclease activity [ISSLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MAIKSLESFL FERGLVGSYA IEALNNCTLD IDVNHYVSRL LTNKREQYLD AIGGFPTSLK  60
   61 MYLESDLKIF KDFNITPIFV FNGGLTYNQL EASGHFTAAS ASASISSTTT SSSGTNATTR 120
  121 SNTESVLLQR SRGWTQWNNL ISSNQNSYID QPIQPQEPFR HNTTIDSKAY QNDLIAYFIE 180
  181 HGYMYQVAPY SSWFQLAYLL NSAYIDAIYG PTDCLMLDCV DRFILGMEFP NKEFRFIDRS 240
  241 RVMKDLGCTH EEFIDIAMAV GNDLQPTTLP PLQIYPVPQL FDIALEMVLN TGTNFYAYQL 300
  301 STTLQNDSKE NIQNYQRGIS ALRYMPVLKD TGKVELFVQE IVVSEEDSEK NNKDGKKSNL 360
  361 SSPSSASSSA SPATTVTKNA SEKLTYEKSS TKEVRKPRDI PNDVHDFIGQ MLPHEYYFYR 420
  421 SIGLVTGKLF DAIVTGVYPE EPPLGGGSST SYKKLVSKSV EIFKNKEINL LTQPINRYYQ 480
  481 IKQIKQVKWY AANEPTTLTN RMSPSMFETI NHLIVKTETS DEKEFSISEF ITTINGSSNM 540
  541 AKDFISEKVI FPNSVPIESK LNSPFNLLST NFLRLLVLLE FFTFDFKEKL LEPTRWGEVF 600
  601 LKLNELNIDS KYHESVIIFL VFLKCDVLKL DEEVQPPAPS ALSQATLRSY PEESLYVLLI 660
  661 TRVLTLFQVD QKPSNYHGPI DKKTLIFRDH LSFIKENLNE LFEAVLISSL TSGEFNRLSL 720
  721 DNFGWARKIV RYLPFKLDSP NTIMAMMWEF FLQKYLHNGN AKNDALSLVA TEFNTYKSTP 780
  781 NLDEQFVESH RFLLEISKVM QELNAAKLID ENVFKLFTKA VEFTTTALSS

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) MKT1 YHB1
    View Details Krogan NJ, et al. (2006) DRS1 ERB1 HAA1 HRQ1 MKT1 STE20 UBP1
    View Details Gavin AC, et al. (2006) MKT1 RPL13A VID24
    View Details Gavin AC, et al. (2002) ARO1 CCT8 CLU1 CPA2 GCN20 GFA1 GUS1 HSC82 IDH2 ILV1 KAP123 MKT1 PFK1 RAD51 RAD52 RFA1 RPA135 RPB2 RPN9 RPT3 RPT6 RVB2 YHB1
    View Details Ho Y, et al. (2002) ADH2 ADR1 AHA1 ARP2 ARP7 BBC1 BCK1 CDC16 CDC33 CDC39 CDC53 CDC55 CLU1 CPR6 CRM1 CYS4 DIG1 DIG2 DUR1,2 ECM29 ECM33 EGD2 ERB1 FAA1 FAA4 FET4 FOL2 FPR3 GAL3 GAL7 GCN1 GCN3 GFA1 GND1 GPG1 GPH1 GUF1 GUS1 HAS1 HHF1, HHF2 HRT1 HTA2 HTB1 HTB2 HXT6 HYP2 IDH1 IDH2 ILV5 IPP1 KIC1 KIN2 KRE33 KRI1 KSS1 LHS1 LOC1 LYS12 MAM33 MDN1 MER1 MES1 MKK2 MKT1 MMS1 MMS4 MRE11 MSE1 MUS81 MYO2 NAP1 NAS6 NHP2 NOP12 NOP2 OYE2 PDC6 PFK1 PHO84 PIL1 PIM1 PMA1 PMA2 POR1 PPE1 PPH22 PWP1 PYC1 QCR2 RAD50 RAD53 RCN2 RGC1 RHO2 RNQ1 RNR1 RNR3 RPA135 RPA190 RPB11 RPC10 RPF2 RPN1 RPN10 RPN11 RPN12 RPN14 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RTS1 RTS3 RVB1 SCP160 SEC23 SEN1 SIS1 SKG3 SLC1 SLT2 SMK1 SSL2 STE12 STE7 STI1 TAF14 TAP42 TEC1 TIF1, TIF2 TPD3 TPS1 UBC12 UBI4 UTP22 VMA8 VPS13 VPS21 XRS2 YAR009C YDR239C YGP1 YGR067C YHB1 YHM2 YHR033W YLR035C-A YNL134C YRA1
    View Details Qiu et al. (2008) ECM32 MKT1 NAM7 PBP1 SSB1 SSB2

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run BIR1 No Comments Widlund PO, et al. (2005)
    View Run BIR1 No Comments Widlund PO, et al. (2005)
    View Run BIR1 No Comments Widlund PO, et al. (2005)
    View Run RPA135 his-HA tag on RPA135 Schneider, DA, et al. (2006)
    View Run CEP3 sample cbf3 from feb 2005 Sandall S, et all (2006)
    View Run RPA135 No Comments Schneider, DA, et al. (2006)
    View Run ZDS1 Sample 2- cpn2 from june 2005 McCusker D, et al (2007)
    View Run ZDS1 Sample 3 - cpn + atp, from june 2005 McCusker D, et al (2007)
    View Run MLP2 #03 Alpha Factor Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #29 Asynchronous Prep5-TiO2 Flowthrough Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data MKT1 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..84]
    [100..248]
    [303..372]    		 PSI-BLAST 56.30103 Flap endonuclease-1 (Fen-1 nuclease)
    2 View Details [85..99]
    [249..302]    		 PSI-BLAST 56.30103 Flap endonuclease-1 (Fen-1 nuclease)
    3 View Details [373..439] PSI-BLAST 12.522879 Exonuclease domain of prokaryotic DNA polymerase; DNA polymerase I (Klenow fragment)
    4 View Details [440..562] deduced N/A No confident structure predictions are available.
    5 View Details [563..830] MSA 1.005883 View MSA. No confident structure predictions are available.

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • endodeoxyribonuclease activity
    		•  4.18461263107599 bayes_pls_golite062009
  • deoxyribonuclease activity
    		•  3.85892177341967 bayes_pls_golite062009
  • exonuclease activity
    		•  3.45142689336533 bayes_pls_golite062009
  • flap endonuclease activity
    		•  3.15967964087761 bayes_pls_golite062009
  • exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters
    		•  2.42937586057619 bayes_pls_golite062009
  • exodeoxyribonuclease activity, producing 5'-phosphomonoesters
    		•  2.28283401034201 bayes_pls_golite062009
  • exodeoxyribonuclease activity
    		•  2.26089375411362 bayes_pls_golite062009
  • nuclease activity
    		•  2.25783328399886 bayes_pls_golite062009
  • 5'-3' exonuclease activity
    		•  2.09065411241765 bayes_pls_golite062009
  • DNA binding
    		•  1.80079106105882 bayes_pls_golite062009
  • nucleic acid binding
    		•  1.77028256579181 bayes_pls_golite062009
  • protein binding
    		•  1.53930087292625 bayes_pls_golite062009
  • endonuclease activity
    		•  1.42881364561524 bayes_pls_golite062009
  • binding
    		•  1.42844458880206 bayes_pls_golite062009
  • 5'-3' exodeoxyribonuclease activity
    		•  1.40692088927452 bayes_pls_golite062009
  • catalytic activity
    		•  1.37534898662234 bayes_pls_golite062009
  • double-stranded DNA specific exodeoxyribonuclease activity
    		•  1.20639149622758 bayes_pls_golite062009
  • 5'-flap endonuclease activity
    		•  0.995401458224216 bayes_pls_golite062009
  • hydrolase activity, acting on ester bonds
    		•  0.931536010162285 bayes_pls_golite062009
  • endodeoxyribonuclease activity, producing 5'-phosphomonoesters
    		•  0.592871503540176 bayes_pls_golite062009
  • damaged DNA binding
    		•  0.571529443801057 bayes_pls_golite062009
  • single-stranded DNA specific 5'-3' exodeoxyribonuclease activity
    		•  0.380267633493762 bayes_pls_golite062009
  • structure-specific DNA binding
    		•  0.274441095399626 bayes_pls_golite062009
    2 No functions predicted.
    3 No functions predicted.
    4 No functions predicted.
    5 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.96

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle