Protein Overview: RPN11

General Information:
Name(s) found:	RPN11 / YFR004W [SGD]
Description(s) found: Found 35 descriptions. SHOW ALL	Metalloprotease subunit of the 19S regulatory particle of the 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes [SGD]
Organism:	Saccharomyces cerevisiae
Length:	306 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] proteasome regulatory particle, lid subcomplex [IDA][IPI]
Biological Process:	ubiquitin-dependent protein catabolic process [TAS ]
Molecular Function:	endopeptidase activity [TAS ]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MERLQRLMMN SKVGSADTGR DDTKETVYIS SIALLKMLKH GRAGVPMEVM GLMLGEFVDD  60
   61 YTVNVVDVFA MPQSGTGVSV EAVDDVFQAK MMDMLKQTGR DQMVVGWYHS HPGFGCWLSS 120
  121 VDVNTQKSFE QLNSRAVAVV VDPIQSVKGK VVIDAFRLID TGALINNLEP RQTTSNTGLL 180
  181 NKANIQALIH GLNRHYYSLN IDYHKTAKET KMLMNLHKEQ WQSGLKMYDY EEKEESNLAA 240
  241 TKSMVKIAEQ YSKRIEEEKE LTEEELKTRY VGRQDPKKHL SETADETLEN NIVSVLTAGV 300
  301 NSVAIK

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		CDC39 ECM29 GSG1 NAS6 OSH7 POL4 PRE5 RPN1 RPN10 RPN11 RPN12 RPN13 RPN14 RPN2 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6 SEC18 SEM1 SLX5 SPG5 SWA2 TOD6 UBP6
View Details	(MIPS) Mewes HW, et al. (2004)		NAS6 RPN1 RPN10 RPN11 RPN12 RPN2 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6
View Details	Krogan NJ, et al. (2006)		CSI1 CSN9 ERV46 FPR2 HUL5 IQG1 MOT1 MPT5 NAS6 NMD3 NPT1 PCI8 PEP1 PST1 PUS9 RPN1 RPN10 RPN11 RPN12 RPN13 RPN14 RPN2 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6 RRI1 RRI2 SEC18 SEM1 SKP1 SPG5 SPO77 SQT1 SWA2 TCB2 TIP41 TOD6 TOR2 TSR4 UBP6 YEN1 YKL027W YMR258C YNL022C YPR003C YPT52
View Details	Gavin AC, et al. (2006)		ECM29 RPN1 RPN10 RPN11 RPN12 RPN13 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1
View Details	Gavin AC, et al. (2002)		ACC1 APM1 BLM10 CCT8 CDC6 CLU1 CPA2 CYM1 DOA4 DUR1,2 ECM29 FDH1 GCN20 GFA1 GUS1 HSP42 ILV1 KAP123 LAT1 LPD1 LYS12 MLH2 MRPL3 NAS6 PDA1 PDB1 PDX1 PGK1 PRE1 PRE10 PRE2 PRE3 PRE4 PRE5 PRE6 PRE7 PRE8 PRE9 PRP46 PSA1 PUP3 RGR1 RPA135 RPB2 RPN10 RPN11 RPN12 RPN13 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6 SAM1 SCL1 SEC27 SMC3 TCB1 TCM62 TIF1, TIF2 TSA1 TUB3 UBP6 URA7 VAS1 YFL006W YOL098C
View Details	Ho Y, et al. (2002)		AAC1 AAC3 ADE5,7 AHA1 ARC1 ARO1 ARP2 ATG11 ATG12 ATG17 CAR2 CPA2 CPR6 FET4 GCD11 GFA1 GUS1 IDH2 IPP1 LHS1 MDN1 MKT1 NAS6 PPX1 PRB1 PTC7 REP1 REX2 RFC3 RFC4 RFC5 RPN1 RPN10 RPN11 RPN12 RPN13 RPN14 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 SEC18 SHM2 STI1 TCB1 TIF1, TIF2 TMA29 TYR1 UBC12 YCL042W YHR020W YHR033W YNL208W
View Details	Qiu et al. (2008)		NAS6 PRE1 PRE10 PRE2 PRE3 PRE4 PRE5 PRE6 PRE7 PRE8 PRE9 PUP2 RPN1 RPN10 RPN11 RPN12 RPN13 RPN2 RPN3 RPN5 RPN6 RPN7 RPN8 RPN9 RPT1 RPT2 RPT3 RPT4 RPT5 RPT6 SCL1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	BOI1	Sample boi 1 with ha tag from october 2005	McCusker D, et al (2007)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	RPN11	PEX18	2	Unpublished Fields Lab Data
View Screen	RPN11	TPA1	2	Unpublished Fields Lab Data
View Screen	RPN11	RPN8	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	RPN11	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..159]	Pfam	44.958607	Mov34/MPN/PAD-1 family No confident structure predictions are available.
2	View Details	[160..211]	deduced	N/A	Confident ab initio structure predictions are available.
3	View Details	[212..306]	MSA	1.045968	View MSA. Confident ab initio structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2