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Protein Overview: RPB2

General Information:
Name(s) found: RPB2 / YOR151C [SGD]
Description(s) found:
Found 114 descriptions.
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  • RNA polymerase II second largest subunit B150, part of central core; similar to bacterial beta subunit [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 1224 amino acids

    Gene Ontology:
    Cellular Component: mitochondrion [IDALink to PubMed article]
    DNA-directed RNA polymerase II, core complex [TASLink to PubMed article]
    Biological Process: transcription from RNA polymerase II promoter [TASLink to PubMed article]
    Molecular Function: DNA-directed RNA polymerase activity [TASLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSDLANSEKY YDEDPYGFED ESAPITAEDS WAVISAFFRE KGLVSQQLDS FNQFVDYTLQ  60
   61 DIICEDSTLI LEQLAQHTTE SDNISRKYEI SFGKIYVTKP MVNESDGVTH ALYPQEARLR 120
  121 NLTYSSGLFV DVKKRTYEAI DVPGRELKYE LIAEESEDDS ESGKVFIGRL PIMLRSKNCY 180
  181 LSEATESDLY KLKECPFDMG GYFIINGSEK VLIAQERSAG NIVQVFKKAA PSPISHVAEI 240
  241 RSALEKGSRF ISTLQVKLYG REGSSARTIK ATLPYIKQDI PIVIIFRALG IIPDGEILEH 300
  301 ICYDVNDWQM LEMLKPCVED GFVIQDRETA LDFIGRRGTA LGIKKEKRIQ YAKDILQKEF 360
  361 LPHITQLEGF ESRKAFFLGY MINRLLLCAL DRKDQDDRDH FGKKRLDLAG PLLAQLFKTL 420
  421 FKKLTKDIFR YMQRTVEEAH DFNMKLAINA KTITSGLKYA LATGNWGEQK KAMSSRAGVS 480
  481 QVLNRYTYSS TLSHLRRTNT PIGRDGKLAK PRQLHNTHWG LVCPAETPEG QACGLVKNLS 540
  541 LMSCISVGTD PMPIITFLSE WGMEPLEDYV PHQSPDATRV FVNGVWHGVH RNPARLMETL 600
  601 RTLRRKGDIN PEVSMIRDIR EKELKIFTDA GRVYRPLFIV EDDESLGHKE LKVRKGHIAK 660
  661 LMATEYQDIE GGFEDVEEYT WSSLLNEGLV EYIDAEEEES ILIAMQPEDL EPAEANEEND 720
  721 LDVDPAKRIR VSHHATTFTH CEIHPSMILG VAASIIPFPD HNQSPRNTYQ SAMGKQAMGV 780
  781 FLTNYNVRMD TMANILYYPQ KPLGTTRAME YLKFRELPAG QNAIVAIACY SGYNQEDSMI 840
  841 MNQSSIDRGL FRSLFFRSYM DQEKKYGMSI TETFEKPQRT NTLRMKHGTY DKLDDDGLIA 900
  901 PGVRVSGEDV IIGKTTPISP DEEELGQRTA YHSKRDASTP LRSTENGIVD QVLVTTNQDG 960
  961 LKFVKVRVRT TKIPQIGDKF ASRHGQKGTI GITYRREDMP FTAEGIVPDL IINPHAIPSR1020
 1021 MTVAHLIECL LSKVAALSGN EGDASPFTDI TVEGISKLLR EHGYQSRGFE VMYNGHTGKK1080
 1081 LMAQIFFGPT YYQRLRHMVD DKIHARARGP MQVLTRQPVE GRSRDGGLRF GEMERDCMIA1140
 1141 HGAASFLKER LMEASDAFRV HICGICGLMT VIAKLNHNQF ECKGCDNKID IYQIHIPYAA1200
 1201 KLLFQELMAM NITPRLYTDR SRDF

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) ASR1 CLU1 DST1 IWR1 RPA135 RPB10 RPB11 RPB2 RPB3 RPB4 RPB5 RPB7 RPB8 RPB9 RPO21 RPO26 SAM1 SPT4 SPT5 TAF14 TFG1 TFG2
    View Details (MIPS) Mewes HW, et al. (2004) CDC73 RPB10 RPB11 RPB2 RPB3 RPB4 RPB5 RPB7 RPB8 RPB9 RPC10 RPO21 RPO26
    View Details Krogan NJ, et al. (2006) ALG3 BUD27 CBF2 DST1 ESS1 IWR1 RPB10 RPB11 RPB2 RPB3 RPB4 RPB5 RPB7 RPB8 RPB9 RPO21 RPO26 SPT4 TAF14 TFG1 TFG2 YBL059W YBR259W YPL225W
    View Details Gavin AC, et al. (2006) IWR1 RPB11 RPB2 RPB3 RPB4 RPB7 RPB9 TFG1 TFG2
    View Details Gavin AC, et al. (2002) ABD1 ACC1 ADA2 ADH1 AOS1 ARO1 CCT8 CLU1 CPA2 FDH1 FUN19 GCN20 GCN5 GFA1 GUS1 HEM13 HFI1 HSC82 IDH2 ILV1 IWR1 KAP114 KAP123 LYS12 MED4 MES1 MKT1 MNN4 MOT1 MRPL3 MYO1 MYO4 NGG1 NOP1 PFK1 PFK2 PSA1 PSK1 PTC1 PUF6 RAD51 RAD52 RFA1 RPA135 RPB11 RPB2 RPB3 RPB4 RPB5 RPB7 RPB8 RPB9 RPN10 RPN11 RPN12 RPN8 RPN9 RPO21 RPO26 RPT3 RPT5 RPT6 RVB2 SAM1 SEC27 SGF29 SGF73 SIN3 SIN4 SMC3 SPT15 SPT20 SPT4 SPT5 SPT7 SPT8 TAF1 TAF10 TAF11 TAF12 TAF13 TAF14 TAF2 TAF3 TAF4 TAF5 TAF6 TAF7 TAF8 TAF9 TBF1 TCB1 TEF4 TFG1 TFG2 TIF1, TIF2 TSA1 TUB3 UBP8 URA7 VAS1 VPS1 YHB1 YOL098C
    View Details Qiu et al. (2008) RET1 RPB10 RPB11 RPB2 RPB3 RPB4 RPB5 RPB7 RPB8 RPB9 RPC10 RPC19 RPC34 RPC40 RPC82 RPO21 RPO26 TAF14

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run RBA50 No Comments Hazbun TR, et al. (2003)
    View Run YMR185W No Comments Hazbun TR, et al. (2003)
    View Run TOR2 No Comments Reinke A, et al. (2004)
    View Run RPA135 his-HA tag on RPA135 Schneider, DA, et al. (2006)
    View Run RPA135 his-HA tag on RPA135 Schneider, DA, et al. (2006)
    View Run RPA135 his-HA tag on RPA135 Schneider, DA, et al. (2006)
    View Run CEP3 sample cbf3 from feb 2005 Sandall S, et all (2006)
    View Run RPA135 No Comments Schneider, DA, et al. (2006)
    View Run ZDS1 Sample 3 - cpn + atp, from june 2005 McCusker D, et al (2007)
    View Run BOI1 Sample boi 1 with ha tag from october 2005 McCusker D, et al (2007)
    View Run MLP2 #03 Alpha Factor Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #02 Alpha Factor Prep1-TiO2 enriched, new search criteria Keck JM, et al. (2011)
    View Run MLP2 #30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #11 Mitotic Prep1-TiO2 enriched, new search criteria Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

      BAIT PREY HITS PUBLICATION
    View Screen RPB2 PPZ2 2 Unpublished Fields Lab Data

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data RPB2 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..220]
    [398..551]
    [598..750]    		 PSI-BLAST 11000.0 RBP2
    2 View Details [221..397]
    [552..597]
    [751..1128]    		 PSI-BLAST 11000.0 RBP2
    3 View Details [1129..1224] PSI-BLAST 11000.0 RBP2

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • DNA-directed RNA polymerase activity
    		•  10.2021663255698 bayes_pls_golite062009
  • RNA polymerase activity
    		•  10.2021663255698 bayes_pls_golite062009
  • nucleotidyltransferase activity
    		•  7.6240729546188 bayes_pls_golite062009
  • general RNA polymerase II transcription factor activity
    		•  6.31198922553685 bayes_pls_golite062009
  • transferase activity, transferring phosphorus-containing groups
    		•  5.44374972993429 bayes_pls_golite062009
  • transferase activity
    		•  4.83218811360621 bayes_pls_golite062009
  • transcription regulator activity
    		•  2.34884279395902 bayes_pls_golite062009
  • nucleic acid binding
    		•  2.33174616086951 bayes_pls_golite062009
  • DNA binding
    		•  2.27079409864244 bayes_pls_golite062009
  • binding
    		•  1.73416306650822 bayes_pls_golite062009
  • RNA polymerase II transcription mediator activity
    		•  1.53259746846673 bayes_pls_golite062009
  • catalytic activity
    		•  1.36406839864424 bayes_pls_golite062009
  • RNA binding
    		•  1.12618950637766 bayes_pls_golite062009
  • RNA polymerase II transcription factor activity
    		•  0.847783043631028 bayes_pls_golite062009
  • protein binding
    		•  0.45174975126993 bayes_pls_golite062009
    2 No functions predicted.
    3 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.98

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle