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Bait Protein: RPA135
Organism: Saccharomyces cerevisiae
Publication: Schneider DA, French SL, Osheim YN, Bailey AO, Vu L, Dodd J, Yates JR, Beyer AL, Nomura M. RNA polymerase II elongation factors Spt4p and Spt5p play roles in transcription elongation by RNA polymerase I and rRNA processing. Proc Natl Acad Sci U S A. 2006;103(34):12707-12.
Comments: his-HA tag on RPA135

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  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   SUP35 Translation termination factor eRF3; altered protein conformation creates the [PSI(+)] prion, a domi... 42 111 55.5% 0.0372 76551
[View Peptides]   RPA190 RNA polymerase I largest subunit A190 91 190 53.8% 0.0262 186431
[View Peptides]   RPA12 RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is resp... 6 12 53.6% 0.0221 13661
[View Peptides]   RPC40 RNA polymerase subunit AC40, common to RNA polymerase I and III 16 31 50.7% 0.0213 37687
[View Peptides]   RPB4 RNA polymerase II subunit B32; forms two subunit dissociable complex with Rpb7p; involved recruitmen... 12 20 49.8% 0.0208 25414
[View Peptides]   IBI2 Protein of unknown function that associates with ribosomes; green fluorescent protein (GFP)-fusion p... 4 4 48.7% 0.00467 22179
[View Peptides]   CMD1 Calmodulin; Ca++ binding protein that regulates Ca++ independent processes (mitosis, bud growth, act... 3 3 47.6% 0.00469 16135
[View Peptides]   RPO26 RNA polymerase subunit ABC23, common to RNA polymerases I, II, and III; part of central core; simila... 4 11 45.2% 0.0163 17910
[View Peptides]   RPA34 RNA polymerase I subunit A34.5 12 20 44.2% 0.0197 26875
[View Peptides]   RPC19 RNA polymerase subunit AC19, common to RNA polymerases I and III 4 6 43.7% 0.00971 16151
[View Peptides]   RPP2A Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction... 4 9 43.4% 0.0195 10746
[View Peptides]   SPT5 Protein involved in regulating Pol I and Pol II transcription and pre-mRNA processing; forms a compl... 55 88 42.4% 0.019 115650
[View Peptides]   RPA135 RNA polymerase I second largest subunit A135 38 95 42.1% 0.0181 135742
[View Peptides]   RPA49 RNA polymerase I subunit A49 21 44 41.9% 0.0244 46651
[View Peptides]   RPB5 RNA polymerase subunit ABC27, common to RNA polymerases I, II, and III; contacts DNA and affects tra... 9 18 40.5% 0.0192 25079
[View Peptides]   RPC10 RNA polymerase subunit ABC10-alpha, found in RNA polymerase complexes I, II, and III 2 4 40.0% 0.0131 7716
[View Peptides]   RPB3 RNA polymerase II third largest subunit B44, part of central core; similar to prokaryotic alpha subu... 7 18 39.0% 0.013 35298
[View Peptides]   DDR48 DNA damage-responsive protein, expression is increased in response to heat-shock stress or treatment... 9 10 37.7% 0.00534 46233
[View Peptides]   RPA43 RNA polymerase I subunit A43 9 48 33.1% 0.0338 36224
[View Peptides]   ACT1 Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functio... 7 13 32.0% 0.00797 41690
[View Peptides]   RPO21 RNA polymerase II largest subunit B220, part of central core; phosphorylation of C-terminal heptapep... 44 62 31.7% 0.00822 191610
[View Peptides]   RPB2 RNA polymerase II second largest subunit B150, part of central core; similar to bacterial beta subun... 29 56 30.1% 0.0105 138751
[View Peptides]   DEF1 RNAPII degradation factor, forms a complex with Rad26p in chromatin, enables ubiquitination and prot... 21 30 27.5% 0.00934 83973
[View Peptides]   SSC1 Hsp70 family ATPase, constituent of the import motor component of the Translocase of the Inner Mitoc... 16 21 26.3% 0.00738 70628
[View Peptides]   PIN3 Protein that induces appearance of [PIN+] prion when overproduced 3 3 26.0% 0.00321 23539
[View Peptides]   LSB1 Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldri... 3 3 24.1% 0.00286 26139
[View Peptides]   PDC1 Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates... 7 8 22.7% 0.00327 61495
[View Peptides]   SSE1 ATPase that is a component of the heat shock protein Hsp90 chaperone complex; binds unfolded protein... 11 11 21.9% 0.00365 77367
[View Peptides]   ENO2 Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... 6 11 21.5% 0.00578 46914
[View Peptides]   RPB8 RNA polymerase subunit ABC14.5, common to RNA polymerases I, II, and III 2 2 21.2% 0.00315 16511
[View Peptides]   RPP2B Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction ... 2 2 20.0% 0.00418 11050
[View Peptides]   TEF1, TEF2 Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... 5 6 19.4% 0.00301 50033
[View Peptides]   TEF1, TEF2 Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... 5 6 19.4% 0.00301 50033
[View Peptides]   RPB11 RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit 2 2 19.2% 0.00383 13616
[View Peptides]   RPA14 RNA polymerase I subunit A14 2 2 19.0% 0.00335 14585
[View Peptides]   YEL007W Putative protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) 7 10 18.5% 0.00345 72534
[View Peptides]   BRE5 Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde ... 5 7 16.7% 0.00312 57674
[View Peptides]   YEF3 Translational elongation factor 3, stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes b... 7 8 14.9% 0.00176 115945
[View Peptides]   SOK2 Nuclear protein that plays a regulatory role in the cyclic AMP (cAMP)-dependent protein kinase (PKA)... 5 5 14.5% 0.00146 85643
[View Peptides]   YPR137C-A, YAR010C Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 3 3 14.5% 0.00157 49072
[View Peptides]   YDR365W-A, YPL25... Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 3 3 14.5% 0.00157 48953
[View Peptides]   YDR365W-A, YPL25... Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 3 3 14.5% 0.00157 48953
[View Peptides]   YDR261C-C Retrotransposon TYA Gag gene co-transcribed with TYB Pol; in YDRCTY1-3 TYB is mutant and probably no... 3 3 14.5% 0.00157 49009
[View Peptides]   ENO1 Enolase I, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosph... 4 8 14.2% 0.00421 46816
[View Peptides]   RNQ1 [PIN(+)] prion, an infectious protein conformation that is generally an ordered protein aggregate 3 6 13.3% 0.0034 42580
[View Peptides]   RPP0 Conserved ribosomal protein P0 similar to rat P0, human P0, and E. coli L10e; shown to be phosphoryl... 3 4 13.1% 0.00295 33717
[View Peptides]   SEC17 Peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' ste... 2 2 13.0% 0.00157 32803
[View Peptides]   UBP3 Ubiquitin-specific protease that interacts with Bre5p to co-regulate anterograde and retrograde tran... 6 6 12.9% 0.00151 101917
[View Peptides]   YOR192C-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 3 4 12.6% 0.0021 49736
[View Peptides]   YDR261W-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 3 4 12.6% 0.0021 49724
[View Peptides]   YDR210W-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 3 4 12.6% 0.0021 49835
[View Peptides]   YCL020W Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 3 4 12.6% 0.0021 49841
[View Peptides]   PIN4 Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contai... 4 4 12.3% 0.00138 73776
[View Peptides]   CDC19 Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... 4 4 11.8% 0.00184 54545
[View Peptides]   ATP1 Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily ... 3 3 11.7% 0.00126 58618
[View Peptides]   YBR012W-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 2 2 11.6% 0.00104 49068
[View Peptides]   YGR038C-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 2 2 11.6% 0.00104 49034
[View Peptides]   YGR027W-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 2 2 11.6% 0.00104 49056
[View Peptides]   YER159C-A, YDR31... Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 2 2 11.6% 0.00104 49129
[View Peptides]   YER159C-A, YDR31... Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 2 2 11.6% 0.00104 49129
[View Peptides]   YDR210C-C Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 2 2 11.6% 0.00104 49187
[View Peptides]   YDR098C-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 2 2 11.6% 0.00104 49057
[View Peptides]   CTS2 Protein similar to Ashbya gossypii sporulation-specific chitinase 3 3 11.0% 0.00135 59118
[View Peptides]   MOT3 Nuclear transcription factor with two Cys2-His2 zinc fingers; involved in repression of a subset of ... 3 3 10.8% 0.00141 54382
[View Peptides]   YPL247C Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to th... 3 3 10.5% 0.00132 57324
[View Peptides]   ATP2 Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily c... 3 3 9.4% 0.00135 54794
[View Peptides]   FAS1 Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty a... 12 12 9.3% 0.00134 228689
[View Peptides]   FKS1 Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic sub... 10 11 9.0% 0.00135 214849
[View Peptides]   FAS2 Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty ... 7 9 8.7% 0.0011 206945
[View Peptides]   YFL002W-B, YGR161W-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 2 3 8.7% 0.00157 49762
[View Peptides]   YFL002W-B, YGR161W-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 2 3 8.7% 0.00157 49762
[View Peptides]   YBL100W-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 2 2 7.5% 0.00105 49898
[View Peptides]   YOR343W-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 2 2 7.5% 0.00105 49868
[View Peptides]   YLR410W-A Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 2 2 7.5% 0.00105 49772
[View Peptides]   YDR034C-C Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; ... 2 2 7.5% 0.00105 49689
[View Peptides]   PGK1 3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phospha... 2 2 7.5% 0.0011 44738
[View Peptides]   DED1 ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all ye... 3 3 7.3% 0.00114 65553
[View Peptides]   SFB3 Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat, required for cargo selection du... 2 2 7.1% 4.95E-4 103950
[View Peptides]   MNN1 Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for add... 2 2 6.8% 6.03E-4 88529
[View Peptides]   VMA2 Subunit B of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), a... 2 2 6.4% 8.89E-4 57749
[View Peptides]   PSP1 Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) an... 3 4 5.7% 0.00109 95348
[View Peptides]   PAN2 Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tai... 3 4 5.5% 8.24E-4 127039
[View Peptides]   HSC82 Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, a... 3 3 5.5% 9.78E-4 80900
[View Peptides]   HSP82 Hsp90 chaperone required for pheromone signaling and negative regulation of Hsf1p; docks with Tom70p... 3 3 5.5% 9.72E-4 81406
[View Peptides]   HSP60 Tetradecameric mitochondrial chaperonin required for ATP-dependent folding of precursor polypeptides... 2 2 5.4% 8.04E-4 60752
[View Peptides]   CHC1 Clathrin heavy chain, subunit of the major coat protein involved in intracellular protein transport ... 5 5 5.3% 6.95E-4 187233
[View Peptides]   SAP185 Protein that forms a complex with the Sit4p protein phosphatase and is required for its function; me... 2 3 4.9% 6.52E-4 121403
[View Peptides]   NUP100 Subunit of the nuclear pore complex (NPC) that is localized to both sides of the pore; contains a re... 2 2 4.8% 4.79E-4 99988
[View Peptides]   GSC2 Catalytic subunit of 1,3-beta-glucan synthase, involved in formation of the inner layer of the spore... 5 5 4.4% 6.06E-4 216988
[View Peptides]   PBP1 Component of glucose deprivation induced stress granules, involved in P-body-dependent granule assem... 2 5 4.4% 0.00159 78782
[View Peptides]   PAN3 Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tai... 2 2 4.4% 6.77E-4 76455
[View Peptides]   PAN1 Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p, associates with actin patches on th... 3 3 4.3% 4.66E-4 160266
[View Peptides]   KAR2 ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... 2 3 4.3% 0.00101 74468
[View Peptides]   KAR2 ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... 2 3 4.3% 0.00101 74468
[View Peptides]   CBK1 Serine/threonine protein kinase that regulates cell morphogenesis pathways; involved in cell wall bi... 2 2 4.2% 6.08E-4 86946
[View Peptides]   NRP1 Putative RNA binding protein of unknown function; localizes to stress granules induced by glucose de... 2 2 4.2% 6.39E-4 79299
[View Peptides]   YDR261C-D Retrotransposon TYA Gag and TYB Pol genes; in YDRCTY1-3 TYB is mutant and probably non-functional 3 3 4.0% 4.3E-4 181660
[View Peptides]   DRS2 Aminophospholipid translocase (flippase) that maintains membrane lipid asymmetry in post-Golgi secre... 2 2 3.8% 3.39E-4 153844
[View Peptides]   YER138C Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 3 3 3.6% 3.93E-4 198561
[View Peptides]   YDR365W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 3 3 3.6% 3.93E-4 198658
[View Peptides]   SLA1 Cytoskeletal protein binding protein required for assembly of the cortical actin cytoskeleton; inter... 2 2 3.3% 3.69E-4 135848
[View Peptides]   YCL019W Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 3 4 3.1% 5.19E-4 202095
[View Peptides]   YOR192C-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 3 4 3.1% 5.19E-4 201983
[View Peptides]   YDR261W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 3 4 3.1% 5.19E-4 201961
[View Peptides]   YDR210W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 3 4 3.1% 5.19E-4 202094
[View Peptides]   NUP116 Subunit of the Nup82 subcomplex of the nuclear pore complex; localized to both sides of the pore; co... 2 2 3.1% 4.13E-4 116234
[View Peptides]   YGR038C-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 2 2.9% 2.62E-4 198520
[View Peptides]   YGR027W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 2 2.9% 2.62E-4 198656
[View Peptides]   YER160C Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 2 2.9% 2.62E-4 198568
[View Peptides]   YDR316W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 2 2.9% 2.62E-4 198526
[View Peptides]   YDR210C-D Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 2 2.9% 2.62E-4 198838
[View Peptides]   YDR098C-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 2 2.9% 2.62E-4 198594
[View Peptides]   YBR012W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 2 2.9% 2.62E-4 198868
[View Peptides]   RPG1 eIF3a subunit of the core complex of translation initiation factor 3 (eIF3), essential for translati... 2 4 2.7% 9.54E-4 110344
[View Peptides]   SEC31 Component of the Sec13p-Sec31p complex of the COPII vesicle coat, required for vesicle formation in ... 2 2 2.4% 3.61E-4 138703
[View Peptides]   YFL002W-A, YGR161W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 3 2.1% 3.9E-4 202038
[View Peptides]   YFL002W-A, YGR161W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 3 2.1% 3.9E-4 202038
[View Peptides]   YOR343W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 2 1.9% 2.6E-4 202160
[View Peptides]   YLR410W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 2 1.9% 2.6E-4 202121
[View Peptides]   YDR034C-D Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 2 1.9% 2.6E-4 201964
[View Peptides]   YBL100W-B Retrotransposon TYA Gag and TYB Pol genes; transcribed/translated as one unit; polyprotein is proces... 2 2 1.9% 2.6E-4 202214

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