Protein Overview: TPS1

General Information:
Name(s) found:	TPS1 / YBR126C [SGD]
Description(s) found: Found 45 descriptions. SHOW ALL	Synthase subunit of trehalose-6-phosphate synthase/phosphatase complex, which synthesizes the storage carbohydrate trehalose; also found in a monomeric form; expression is induced by the stress response and repressed by the Ras-cAMP pathway [SGD]
Organism:	Saccharomyces cerevisiae
Length:	495 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA] alpha,alpha-trehalose-phosphate synthase complex (UDP-forming) [IDA][IGI][IMP ][IPI]
Biological Process:	carbohydrate metabolic process [IDA][IMP] response to drug [IMP] trehalose biosynthetic process [IDA][IMP] response to stress [IDA][IGI][IMP]
Molecular Function:	alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity [IDA][IMP]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTTDNAKAQL TSSSGGNIIV VSNRLPVTIT KNSSTGQYEY AMSSGGLVTA LEGLKKTYTF  60
   61 KWFGWPGLEI PDDEKDQVRK DLLEKFNAVP IFLSDEIADL HYNGFSNSIL WPLFHYHPGE 120
  121 INFDENAWLA YNEANQTFTN EIAKTMNHND LIWVHDYHLM LVPEMLRVKI HEKQLQNVKV 180
  181 GWFLHTPFPS SEIYRILPVR QEILKGVLSC DLVGFHTYDY ARHFLSSVQR VLNVNTLPNG 240
  241 VEYQGRFVNV GAFPIGIDVD KFTDGLKKES VQKRIQQLKE TFKGCKIIVG VDRLDYIKGV 300
  301 PQKLHAMEVF LNEHPEWRGK VVLVQVAVPS RGDVEEYQYL RSVVNELVGR INGQFGTVEF 360
  361 VPIHFMHKSI PFEELISLYA VSDVCLVSST RDGMNLVSYE YIACQEEKKG SLILSEFTGA 420
  421 AQSLNGAIIV NPWNTDDLSD AINEALTLPD VKKEVNWEKL YKYISKYTSA FWGENFVHEL 480
  481 YSTSSSSTSS SATKN

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		TPS1 TPS2 TPS3 TSL1
View Details	Krogan NJ, et al. (2006)		TPS1 TPS2 TPS3 TSL1
View Details	Gavin AC, et al. (2002)		CTR3 DAL3 GLN1 GTO3 IML1 MCM2 MKS1 REG1 TIF1, TIF2 TPS1 TPS2 TPS3 TSC10 TSL1 YIL177C
View Details	Ho Y, et al. (2002)		AAT2 ACS2 ADE17 ADH4 ADK1 ADR1 ALA1 APT1 ARG4 ARO1 ATP3 ATP5 ATP7 BBC1 BFR2 BIR1 BNA5 CAR2 CDC14 CDC25 CDC39 CDC53 CDC60 CDC7 CKI1 CPR1 CRM1 CYR1 DBF2 DED81 DEF1 DPM1 DSS1 DUR1,2 ECM10 ECM29 ECM33 ENP1 ERG10 ERG20 FAA1 FAA4 FUR1 GAL3 GCN1 GCR2 GDE1 GDI1 GIN4 GLC7 GPH1 GRS1 HEF3 HMS1 HOM3 HRT1 HYP2 ILS1 IRA1 IRA2 JIP3 KGD1 KNS1 LSM4 MCR1 MDH1 MDN1 MER1 MKT1 MMS1 MOB1 MPC54 MRS6 MTC5 MYO2 MYO4 NAP1 NAS6 NPA3 NUM1 NUT1 OYE2 PAA1 PAT1 PDC5 PDC6 PFK1 PGM2 PMA2 PMD1 PRE1 PRO2 PRP6 PRS2 PRS3 PRS5 PST2 PTC4 PTC6 PUP3 PYC1 RAD1 RIM11 RPA190 RPC19 RPN1 RPN5 RPT3 RPT5 SAR1 SEC27 SEF1 SKT5 SNF4 SOD2 SPE3 SPT16 SSZ1 STI1 SUI1 SYF1 TFP1 THI3 THR4 TIF1, TIF2 TMA29 TPS1 TRR1 TSA2 TSL1 UBI4 UBP15 VAC14 VAS1 VHS1 VPS13 VPS21 YAR009C YDL124W YDR170W-A YER138C YER160C YGP1 YHR033W YJR027W YJR028W YKU80 YLR035C-A
View Details	Qiu et al. (2008)		AST2 GIP2 GLK1 REG1 TKL2 TPS1 TPS2 TPS3 TSL1 YGR149W

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	BOI1	Sample boi 1 with ha tag from october 2005	McCusker D, et al (2007)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	ZIM17	TPS1	2	Unpublished Fields Lab Data
View Screen	NUP1	TPS1	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	TPS1	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..115]	MSA	1.41699	View MSA. No confident structure predictions are available.
2	View Details	[116..263] [463..495]	ORFEUS	13.21	UDP-N-acetylglucosamine 2-epimerase
3	View Details	[264..462]	ORFEUS	13.21	UDP-N-acetylglucosamine 2-epimerase

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
alpha,alpha-trehalose-phosphate synthase (UDP-forming) activity	11.2045941324603	bayes_pls_golite062009
glucosyltransferase activity	8.25064561355471	bayes_pls_golite062009
UDP-glucosyltransferase activity	8.0463972704113	bayes_pls_golite062009
trehalose-phosphatase activity	7.58500038184065	bayes_pls_golite062009
transferase activity, transferring hexosyl groups	3.70184884300914	bayes_pls_golite062009
catalytic activity	2.65148466065976	bayes_pls_golite062009
transferase activity, transferring glycosyl groups	2.62403914335522	bayes_pls_golite062009
transferase activity	2.18054407163329	bayes_pls_golite062009
UDP-glycosyltransferase activity	2.13085705769565	bayes_pls_golite062009
UDP-N-acetylglucosamine 2-epimerase activity	1.55280519670033	bayes_pls_golite062009
carbohydrate phosphatase activity	0.78952375993773	bayes_pls_golite062009
glycogen phosphorylase activity	0.607220464589157	bayes_pls_golite062009
binding	0.345236672370351	bayes_pls_golite062009
phosphorylase activity	0.296436712681472	bayes_pls_golite062009
mannosyltransferase activity	0.253888301381021	bayes_pls_golite062009
protein binding	0.198267161626932	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2