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Bait Protein: BIR1
Organism: Saccharomyces cerevisiae
Publication: Widlund PO, et al. (2005) Phosphorylation of the Chromosomal Passenger Protein, Bir1, Is Required for Localization of Ndc10 to the Spindle during Anaphase and Full Spindle Elongation. Mol Biol Cell [Epub ahead of print]
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  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   ENO2 Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosp... 19 22 54.2% 0.0125 46914
[View Peptides]   BIR1 Subunit of chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), which regulates chromosome... 36 48 44.0% 0.0125 108667
[View Peptides]   PDC1 Major of three pyruvate decarboxylase isozymes, key enzyme in alcoholic fermentation, decarboxylates... 12 18 32.3% 0.00797 61495
[View Peptides]   PGK1 3-phosphoglycerate kinase, catalyzes transfer of high-energy phosphoryl groups from the acyl phospha... 8 10 30.3% 0.00599 44738
[View Peptides]   FBA1 Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversio... 7 9 29.0% 0.00625 39621
[View Peptides]   CDC19 Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... 7 7 23.8% 0.00349 54545
[View Peptides]   ASC1 G-protein beta subunit and guanine nucleotide dissociation inhibitor for Gpa2p; ortholog of RACK1 th... 3 3 22.6% 0.00234 34805
[View Peptides]   PSA1 GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase), synthesizes GDP-mannose from ... 4 4 19.9% 0.00276 39566
[View Peptides]   TEF1, TEF2 Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... 6 7 14.6% 0.00381 50033
[View Peptides]   TEF1, TEF2 Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... 6 7 14.6% 0.00381 50033
[View Peptides]   SLX9 Protein required for pre-rRNA processing; associated with the 90S pre-ribosome and 43S small ribosom... 2 3 13.8% 0.00356 24069
[View Peptides]   PET9 Major ADP/ATP carrier of the mitochondrial inner membrane, exchanges cytosolic ADP for mitochondrial... 2 2 12.9% 0.00157 34426
[View Peptides]   DED1 ATP-dependent DEAD (Asp-Glu-Ala-Asp)-box RNA helicase, required for translation initiation of all ye... 4 4 12.6% 0.00165 65553
[View Peptides]   PBP1 Component of glucose deprivation induced stress granules, involved in P-body-dependent granule assem... 6 7 12.2% 0.00242 78782
[View Peptides]   SRO9 Cytoplasmic RNA-binding protein that associates with translating ribosomes; involved in heme regulat... 3 3 10.9% 0.0016 51789
[View Peptides]   ATP2 Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily c... 3 3 10.8% 0.00146 54794
[View Peptides]   ACT1 Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functio... 2 2 9.9% 0.00133 41690
[View Peptides]   DOC1 Processivity factor required for the ubiquitination activity of the anaphase promoting complex (APC)... 2 2 9.9% 0.00176 32822
[View Peptides]   ADH1 Alcohol dehydrogenase, fermentative isozyme active as homo- or heterotetramers; required for the red... 2 2 9.8% 0.00143 36849
[View Peptides]   GDH1 NADP(+)-dependent glutamate dehydrogenase, synthesizes glutamate from ammonia and alpha-ketoglutarat... 2 2 9.3% 0.0011 49570
[View Peptides]   HXK2 Hexokinase isoenzyme 2 that catalyzes phosphorylation of glucose in the cytosol; predominant hexokin... 2 2 7.8% 0.00103 53943
[View Peptides]   PDI1 Protein disulfide isomerase, multifunctional protein resident in the endoplasmic reticulum lumen, es... 2 2 7.5% 9.55E-4 58227
[View Peptides]   HXK1 Hexokinase isoenzyme 1, a cytosolic protein that catalyzes phosphorylation of glucose during glucose... 2 4 7.2% 0.00206 53738
[View Peptides]   ILV5 Acetohydroxyacid reductoisomerase, mitochondrial protein involved in branched-chain amino acid biosy... 2 2 7.1% 0.00126 44368
[View Peptides]   CBF2 Essential kinetochore protein, component of the CBF3 multisubunit complex that binds to the CDEIII r... 4 4 7.0% 0.00104 111917
[View Peptides]   EFT2, EFT1 Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein s... 3 3 6.2% 8.88E-4 93289
[View Peptides]   EFT2, EFT1 Elongation factor 2 (EF-2), also encoded by EFT1; catalyzes ribosomal translocation during protein s... 3 3 6.2% 8.88E-4 93289
[View Peptides]   PGI1 Glycolytic enzyme phosphoglucose isomerase, catalyzes the interconversion of glucose-6-phosphate and... 2 2 6.1% 9.0E-4 61299
[View Peptides]   BRE5 Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde ... 2 2 5.8% 9.68E-4 57674
[View Peptides]   SLI15 Subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), which regula... 2 3 5.7% 0.00107 79186
[View Peptides]   MKT1 Protein that forms a complex with Pbp1p that may mediate posttranscriptional regulation of HO endonu... 2 2 5.2% 6.0E-4 94494
[View Peptides]   RGA1 GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of se... 3 3 5.2% 7.42E-4 112831
[View Peptides]   HSC82 Cytoplasmic chaperone of the Hsp90 family, redundant in function and nearly identical with Hsp82p, a... 2 2 4.4% 7.07E-4 80900
[View Peptides]   HSP82 Hsp90 chaperone required for pheromone signaling and negative regulation of Hsf1p; docks with Tom70p... 2 2 4.4% 7.03E-4 81406
[View Peptides]   UBI4 Ubiquitin, becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-... 2 10 3.9% 0.00654 42826
[View Peptides]   TIF4631 Translation initiation factor eIF4G, subunit of the mRNA cap-binding protein complex (eIF4F) that al... 2 2 2.3% 5.23E-4 107101

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