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Protein Overview: DIS3

General Information:
Name(s) found: DIS3 / YOL021C [SGD]
Description(s) found:
Found 29 descriptions.
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  • Exosome core complex catalytic subunit; possesses both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase R and to human DIS3 [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 1001 amino acids

    Gene Ontology:
    Cellular Component: cytoplasmic exosome (RNase complex) [IDALink to PubMed article]
    nuclear exosome (RNase complex) [IDALink to PubMed article]
    mitochondrion [IDALink to PubMed articleLink to PubMed article]
    nuclear outer membrane [TASLink to PubMed article]
    Biological Process: mRNA catabolic process [IPILink to PubMed article]
    polyadenylation-dependent ncRNA catabolic process [IGILink to PubMed article]
    Molecular Function: 3'-5'-exoribonuclease activity [IDALink to PubMed article][IMPLink to PubMed article][TASLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSVPAIAPRR KRLADGLSVT QKVFVRSRNG GATKIVREHY LRSDIPCLSR SCTKCPQIVV  60
   61 PDAQNELPKF ILSDSPLELS APIGKHYVVL DTNVVLQAID LLENPNCFFD VIVPQIVLDE 120
  121 VRNKSYPVYT RLRTLCRDSD DHKRFIVFHN EFSEHTFVER LPNETINDRN DRAIRKTCQW 180
  181 YSEHLKPYDI NVVLVTNDRL NREAATKEVE SNIITKSLVQ YIELLPNADD IRDSIPQMDS 240
  241 FDKDLERDTF SDFTFPEYYS TARVMGGLKN GVLYQGNIQI SEYNFLEGSV SLPRFSKPVL 300
  301 IVGQKNLNRA FNGDQVIVEL LPQSEWKAPS SIVLDSEHFD VNDNPDIEAG DDDDNNESSS 360
  361 NTTVISDKQR RLLAKDAMIA QRSKKIQPTA KVVYIQRRSW RQYVGQLAPS SVDPQSSSTQ 420
  421 NVFVILMDKC LPKVRIRTRR AAELLDKRIV ISIDSWPTTH KYPLGHFVRD LGTIESAQAE 480
  481 TEALLLEHDV EYRPFSKKVL ECLPAEGHDW KAPTKLDDPE AVSKDPLLTK RKDLRDKLIC 540
  541 SIDPPGCVDI DDALHAKKLP NGNWEVGVHI ADVTHFVKPG TALDAEGAAR GTSVYLVDKR 600
  601 IDMLPMLLGT DLCSLKPYVD RFAFSVIWEL DDSANIVNVN FMKSVIRSRE AFSYEQAQLR 660
  661 IDDKTQNDEL TMGMRALLKL SVKLKQKRLE AGALNLASPE VKVHMDSETS DPNEVEIKKL 720
  721 LATNSLVEEF MLLANISVAR KIYDAFPQTA MLRRHAAPPS TNFEILNEML NTRKNMSISL 780
  781 ESSKALADSL DRCVDPEDPY FNTLVRIMST RCMMAAQYFY SGAYSYPDFR HYGLAVDIYT 840
  841 HFTSPIRRYC DVVAHRQLAG AIGYEPLSLT HRDKNKMDMI CRNINRKHRN AQFAGRASIE 900
  901 YYVGQVMRNN ESTETGYVIK VFNNGIVVLV PKFGVEGLIR LDNLTEDPNS AAFDEVEYKL 960
  961 TFVPTNSDKP RDVYVFDKVE VQVRSVMDPI TSKRKAELLL K

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) CSL4 DIS3 ECM16 LRP1 MPP6 MTR3 RRP4 RRP40 RRP42 RRP43 RRP45 RRP46 RRP6 SKI6 SKI7 SRP1
    View Details (MIPS) Mewes HW, et al. (2004) DIS3 RRP4 RRP42 RRP43 RRP45 RRP46 SKI6
    View Details Krogan NJ, et al. (2006) CSL4 DDI1 DIS3 ENT3 LRP1 MPP6 MTR3 OSM1 RRP4 RRP40 RRP42 RRP43 RRP45 RRP46 RRP6 SKI6 SKI7 YBL055C
    View Details Gavin AC, et al. (2006) CSL4 DIS3 MTR3 RRP4 RRP40 RRP42 RRP43 RRP45 RRP46 RRP6 SKI6 SKI7
    View Details Gavin AC, et al. (2002) ADE4 ASC1 BFR2 BMS1 BRX1 BUD21 CBF5 CKA1 CKA2 CKB1 CKB2 CMD1 CMS1 CSL4 CTR9 DBP8 DIA4 DIM1 DIP2 DIS3 ECM16 ECM29 EMG1 ENP1 ENP2 ERO1 ESF1 ESF2 FAP7 GCN1 HCA4 HHF1, HHF2 HSL1 HTA2 IMP3 IMP4 ISW1 KRE33 KRR1 LCP5 LRP1 LTV1 MAM33 MAS1 MAS2 MLC1 MPP10 MRD1 MTR3 MVD1 MYO2 NAN1 NMD5 NOB1 NOC2 NOC4 NOP1 NOP14 NOP56 NOP58 NOP6 PNO1 PRE6 PRE9 PRP43 PWP2 RCL1 RML2 ROK1 RPP2B RRP12 RRP4 RRP40 RRP42 RRP43 RRP45 RRP46 RRP6 RRP7 RRP9 RTG2 SAM1 SCL1 SEC21 SHE4 SKI2 SKI3 SKI6 SKI7 SLX9 SMC4 SOF1 SRP1 TIF1, TIF2 TSR1 UTP10 UTP11 UTP13 UTP15 UTP18 UTP20 UTP21 UTP22 UTP30 UTP4 UTP6 UTP7 UTP8 UTP9 YIR035C YOR059C
    View Details Ho Y, et al. (2002) DIS3 EAF3 FIP1 HAS1 HPR1 KAP95 MES1 MFT1 NAM8 NOP6 NUP1 NUP2 NUP60 PAP1 PCT1 REB1 RNT1 RRP4 RRP43 RRP6 RTT103 SIF2 SIN3 SNU56 SRP1 STO1 THO2 TRA1 UME1 YPR090W
    View Details Qiu et al. (2008) CSL4 DIS3 MTR3 POP2 RRP4 RRP40 RRP42 RRP43 RRP45 RRP46 SKI6

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run BOI1 Sample bob1 (2nd set) from october 2005 McCusker D, et al (2007)
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)
    View Run MLP2 #30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

      BAIT PREY HITS PUBLICATION
    View Screen PRP16 DIS3 2 Unpublished Fields Lab Data

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data DIS3 Huh WK, et al. (2003)
    View Data DIS3 and NOP56 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..325] MSA 1.038904 View MSA. No confident structure predictions are available.
    2 View Details [326..415] MSA 1.081995 View MSA. No confident structure predictions are available.
    3 View Details [416..823] Pfam 168.39794 RNB-like protein No confident structure predictions are available.
    4 View Details [824..1001] PSI-BLAST 14.39794 C-terminal domain of RNA polymerase II subunit RBP4 (RpoE); N-terminal, heterodimerisation domain of RBP4 (RpoE)

    Functions predicted (by domain):

    # Gene Ontology predictions
    1 No functions predicted.
    2 No functions predicted.
    3
    Term Confidence Notes
  • ribonuclease activity
    		•  7.42330864261751 bayes_pls_golite062009
  • exoribonuclease activity
    		•  6.76241588209982 bayes_pls_golite062009
  • exoribonuclease activity, producing 5'-phosphomonoesters
    		•  6.73428326869965 bayes_pls_golite062009
  • 3'-5'-exoribonuclease activity
    		•  5.13367897180134 bayes_pls_golite062009
  • 3'-5' exonuclease activity
    		•  3.51306349336452 bayes_pls_golite062009
  • nucleic acid binding
    		•  3.194200758646 bayes_pls_golite062009
  • exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters
    		•  3.07263153665399 bayes_pls_golite062009
  • binding
    		•  2.83465364476231 bayes_pls_golite062009
  • hydrolase activity, acting on ester bonds
    		•  2.73968547121328 bayes_pls_golite062009
  • DNA binding
    		•  2.71213098011194 bayes_pls_golite062009
  • exonuclease activity
    		•  2.66053639652924 bayes_pls_golite062009
  • RNA binding
    		•  2.41160981373213 bayes_pls_golite062009
  • ligase activity, forming aminoacyl-tRNA and related compounds
    		•  2.04350689362549 bayes_pls_golite062009
  • ligase activity, forming carbon-oxygen bonds
    		•  2.04350689362549 bayes_pls_golite062009
  • aminoacyl-tRNA ligase activity
    		•  2.04218282227834 bayes_pls_golite062009
  • structural constituent of ribosome
    		•  2.02605441840546 bayes_pls_golite062009
  • structure-specific DNA binding
    		•  1.81524862921454 bayes_pls_golite062009
  • structural molecule activity
    		•  1.81283970647925 bayes_pls_golite062009
  • single-stranded DNA binding
    		•  1.62996999055328 bayes_pls_golite062009
    		• 1.5765009871268 bayes_pls_golite062009
  • exoribonuclease II activity
    		•  1.18248519339188 bayes_pls_golite062009
  • telomeric DNA binding
    		•  1.15591101342538 bayes_pls_golite062009
  • ligase activity
    		•  1.06979791212194 bayes_pls_golite062009
  • transcription regulator activity
    		•  0.996884322431837 bayes_pls_golite062009
  • hydrolase activity
    		•  0.724860465280978 bayes_pls_golite062009
  • single-stranded telomeric DNA binding
    		•  0.586976595520399 bayes_pls_golite062009
  • nuclease activity
    		•  0.57381531077345 bayes_pls_golite062009
  • aspartate-tRNA ligase activity
    		•  0.504302440847948 bayes_pls_golite062009
  • mRNA binding
    		•  0.4162128308402 bayes_pls_golite062009
  • sequence-specific DNA binding
    		•  0.32084431704638 bayes_pls_golite062009
  • transcription factor activity
    		•  0.298729064067577 bayes_pls_golite062009
  • translation regulator activity
    		•  0.22908707234671 bayes_pls_golite062009
  • chromatin binding
    		•  0.1876135725713 bayes_pls_golite062009
  • translation factor activity, nucleic acid binding
    		•  0.179682348023561 bayes_pls_golite062009
  • protein binding
    		•  0.152645195799531 bayes_pls_golite062009
  • translation initiation factor activity
    		•  0.11625379846973 bayes_pls_golite062009
  • RNA polymerase activity
    		•  0.0740938021136293 bayes_pls_golite062009
  • DNA-directed RNA polymerase activity
    		•  0.0740938021136293 bayes_pls_golite062009
    4 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.98

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle