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Protein Overview: PAP1

General Information:
Name(s) found: PAP1 / YKR002W [SGD]
Description(s) found:
Found 33 descriptions.
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  • Poly(A) polymerase, one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 568 amino acids

    Gene Ontology:
    Cellular Component: mRNA cleavage and polyadenylation specificity factor complex [IPILink to PubMed article]
    nucleoplasm [IPILink to PubMed article]
    Biological Process: mRNA export from nucleus [IMPLink to PubMed article]
    mRNA polyadenylation [IDALink to PubMed article][IMPLink to PubMed article]
    Molecular Function: polynucleotide adenylyltransferase activity [IDALink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSSQKVFGIT GPVSTVGATA AENKLNDSLI QELKKEGSFE TEQETANRVQ VLKILQELAQ  60
   61 RFVYEVSKKK NMSDGMARDA GGKIFTYGSY RLGVHGPGSD IDTLVVVPKH VTREDFFTVF 120
  121 DSLLRERKEL DEIAPVPDAF VPIIKIKFSG ISIDLICARL DQPQVPLSLT LSDKNLLRNL 180
  181 DEKDLRALNG TRVTDEILEL VPKPNVFRIA LRAIKLWAQR RAVYANIFGF PGGVAWAMLV 240
  241 ARICQLYPNA CSAVILNRFF IILSEWNWPQ PVILKPIEDG PLQVRVWNPK IYAQDRSHRM 300
  301 PVITPAYPSM CATHNITEST KKVILQEFVR GVQITNDIFS NKKSWANLFE KNDFFFRYKF 360
  361 YLEITAYTRG SDEQHLKWSG LVESKVRLLV MKLEVLAGIK IAHPFTKPFE SSYCCPTEDD 420
  421 YEMIQDKYGS HKTETALNAL KLVTDENKEE ESIKDAPKAY LSTMYIGLDF NIENKKEKVD 480
  481 IHIPCTEFVN LCRSFNEDYG DHKVFNLALR FVKGYDLPDE VFDENEKRPS KKSKRKNLDA 540
  541 RHETVKRSKS DAASGDNING TTAAVDVN

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) CFT1 CFT2 FIP1 GLC7 MPE1 PAP1 PCF11 PFS2 PTA1 PTI1 REF2 RNA14 SSU72 SWD2 YSH1 YTH1
    View Details Krogan NJ, et al. (2006) AIM1 CFT1 CFT2 ECM21 FIP1 GIP4 MPE1 PAP1 PFS2 PTA1 PTI1 REF2 SSU72 SWD2 SYC1 YBL010C YLR358C YSH1 YTH1
    View Details Gavin AC, et al. (2006) CFT1 CFT2 FIP1 MPE1 PAP1 PFS2 PTA1 YSH1 YTH1
    View Details Gavin AC, et al. (2002) ACT1 ADE4 AIM31 BFR2 BMS1 BUD21 CBF5 CFT1 CFT2 CKA1 CLP1 DBP8 DIP2 ECM16 ECM29 EMG1 ENO2 ENP1 ENP2 ERO1 ESF1 ESF2 FIP1 GCN1 GLC7 HAS1 HCA4 HHF1, HHF2 HSL1 HTA1 HTA2 IMP3 IMP4 KRE33 KRR1 MPE1 MPP10 MVD1 NAN1 NOC4 NOP1 NOP12 NOP14 NOP56 NOP58 NOP6 PAP1 PCF11 PFK1 PFS2 PRE6 PRE9 PRP43 PTA1 PTI1 PWP2 REF2 RML2 RNA14 RNA15 ROK1 RPP2B RRP9 RSA3 RTG2 SCL1 SEC13 SEC21 SEC31 SOF1 SRO9 SSA3 SSU72 SYC1 TAF6 TIF1, TIF2 TIF4632 TYE7 UTP10 UTP11 UTP13 UTP15 UTP18 UTP20 UTP21 UTP22 UTP30 UTP4 UTP6 UTP7 UTP8 UTP9 VID24 VMR1 VPS53 YCL046W YSH1 YTH1
    View Details Ho Y, et al. (2002) DIS3 EAF3 FIP1 HAS1 HPR1 KAP95 MES1 MFT1 NAM8 NOP6 NUP1 NUP2 NUP60 PAP1 PCT1 REB1 RNT1 RRP4 RRP43 RRP6 RTT103 SIF2 SIN3 SNU56 SRP1 STO1 THO2 TRA1 UME1 YPR090W
    View Details Qiu et al. (2008) CFT1 CFT2 CLP1 HRP1 PAP1 PFS2 REF2 RNA14 RNA15 SWD2 YSH1

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run ASF1 3829 - low filter Green EM, et al (2005)
    View Run RPA135 No Comments Schneider, DA, et al. (2006)
    View Run BOI1 Sample bob1 (2nd set) from october 2005 McCusker D, et al (2007)
    View Run MLP2 #24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #32 Asynchronous Prep-No Phosphopeptide enrichment Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data PAP1 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..38]
    [191..353]    		 PSI-BLAST 10166.0 Poly(A) polymerase, middle domain; Poly(A) polymerase N-terminal, catalytic domain; Poly(A) polymerase, C-terminal domain
    2 View Details [39..190]
    [354..568]    		 PSI-BLAST 10166.0 Poly(A) polymerase, middle domain; Poly(A) polymerase N-terminal, catalytic domain; Poly(A) polymerase, C-terminal domain

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • polynucleotide adenylyltransferase activity
    		•  5.40967709450432 bayes_pls_golite062009
  • RNA binding
    		•  2.46555984694381 bayes_pls_golite062009
  • nucleotidyltransferase activity
    		•  2.12021443504286 bayes_pls_golite062009
  • nucleic acid binding
    		•  1.43322212500405 bayes_pls_golite062009
  • transferase activity, transferring phosphorus-containing groups
    		•  1.08480919674453 bayes_pls_golite062009
  • DNA binding
    		•  0.920001549206154 bayes_pls_golite062009
  • binding
    		•  0.888087430062829 bayes_pls_golite062009
  • catalytic activity
    		•  0.75232920999699 bayes_pls_golite062009
  • protein binding
    		•  0.516354942587518 bayes_pls_golite062009
  • transferase activity
    		•  0.432029324907561 bayes_pls_golite062009
    2 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    [View Details]
    Source: Reynolds et al. 2008. Manuscript submitted Philius confidence legend
    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle