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Protein Overview: DNA2

General Information:
Name(s) found: DNA2 / YHR164C [SGD]
Description(s) found:
Found 27 descriptions.
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  • Tripartite DNA replication factor with single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair; cell-cycle dependent localization [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 1522 amino acids

    Gene Ontology:
    Cellular Component: nucleus [TASLink to PubMed article]
    nuclear chromosome, telomeric region [IDALink to PubMed article]
    Biological Process: DNA repair [IGILink to PubMed article][IMPLink to PubMed article]
    lagging strand elongation [IGILink to PubMed articleLink to PubMed article][IMPLink to PubMed articleLink to PubMed article]
    DNA-dependent DNA replication [IDALink to PubMed article][IMPLink to PubMed article]
    telomere maintenance [IMPLink to PubMed article]
    replicative cell aging [IMPLink to PubMed article]
    Molecular Function: 5'-flap endonuclease activity [IDALink to PubMed article]
    ATP-dependent DNA helicase activity [IDALink to PubMed article]
    single-stranded DNA specific endodeoxyribonuclease activity [IDALink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MPGTPQKNKR SASISVSPAK KTEEKEIIQN DSKAILSKQT KRKKKYAFAP INNLNGKNTK  60
   61 VSNASVLKSI AVSQVRNTSR TKDINKAVSK SVKQLPNSQV KPKREMSNLS RHHDFTQDED 120
  121 GPMEEVIWKY SPLQRDMSDK TTSAAEYSDD YEDVQNPSST PIVPNRLKTV LSFTNIQVPN 180
  181 ADVNQLIQEN GNEQVRPKPA EISTRESLRN IDDILDDIEG DLTIKPTITK FSDLPSSPIK 240
  241 APNVEKKAEV NAEEVDKMDS TGDSNDGDDS LIDILTQKYV EKRKSESQIT IQGNTNQKSG 300
  301 AQESCGKNDN TKSRGEIEDH ENVDNQAKTG NAFYENEEDS NCQRIKKNEK IEYNSSDEFS 360
  361 DDSLIELLNE TQTQVEPNTI EQDLDKVEKM VSDDLRIATD STLSAYALRA KSGAPRDGVV 420
  421 RLVIVSLRSV ELPKIGTQKI LECIDGKGEQ SSVVVRHPWV YLEFEVGDVI HIIEGKNIEN 480
  481 KRLLSDDKNP KTQLANDNLL VLNPDVLFSA TSVGSSVGCL RRSILQMQFQ DPRGEPSLVM 540
  541 TLGNIVHELL QDSIKYKLSH NKISMEIIIQ KLDSLLETYS FSIIICNEEI QYVKELVMKE 600
  601 HAENILYFVN KFVSKSNYGC YTSISGTRRT QPISISNVID IEENIWSPIY GLKGFLDATV 660
  661 EANVENNKKH IVPLEVKTGK SRSVSYEVQG LIYTLLLNDR YEIPIEFFLL YFTRDKNMTK 720
  721 FPSVLHSIKH ILMSRNRMSM NFKHQLQEVF GQAQSRFELP PLLRDSSCDS CFIKESCMVL 780
  781 NKLLEDGTPE ESGLVEGEFE ILTNHLSQNL ANYKEFFTKY NDLITKEESS ITCVNKELFL 840
  841 LDGSTRESRS GRCLSGLVVS EVVEHEKTEG AYIYCFSRRR NDNNSQSMLS SQIAANDFVI 900
  901 ISDEEGHFCL CQGRVQFINP AKIGISVKRK LLNNRLLDKE KGVTTIQSVV ESELEQSSLI 960
  961 ATQNLVTYRI DKNDIQQSLS LARFNLLSLF LPAVSPGVDI VDERSKLCRK TKRSDGGNEI1020
 1021 LRSLLVDNRA PKFRDANDDP VIPYKLSKDT TLNLNQKEAI DKVMRAEDYA LILGMPGTGK1080
 1081 TTVIAEIIKI LVSEGKRVLL TSYTHSAVDN ILIKLRNTNI SIMRLGMKHK VHPDTQKYVP1140
 1141 NYASVKSYND YLSKINSTSV VATTCLGIND ILFTLNEKDF DYVILDEASQ ISMPVALGPL1200
 1201 RYGNRFIMVG DHYQLPPLVK NDAARLGGLE ESLFKTFCEK HPESVAELTL QYRMCGDIVT1260
 1261 LSNFLIYDNK LKCGNNEVFA QSLELPMPEA LSRYRNESAN SKQWLEDILE PTRKVVFLNY1320
 1321 DNCPDIIEQS EKDNITNHGE AELTLQCVEG MLLSGVPCED IGVMTLYRAQ LRLLKKIFNK1380
 1381 NVYDGLEILT ADQFQGRDKK CIIISMVRRN SQLNGGALLK ELRRVNVAMT RAKSKLIIIG1440
 1441 SKSTIGSVPE IKSFVNLLEE RNWVYTMCKD ALYKYKFPDR SNAIDEARKG CGKRTGAKPI1500
 1501 TSKSKFVSDK PIIKEILQEY ES

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) DNA2 RFA1 RFA2 RFA3
    View Details (MIPS) Mewes HW, et al. (2004) DNA2 ECM32
    View Details Krogan NJ, et al. (2006) DNA2 HSP12 PRO1 RFA1 RFA2 RFA3
    View Details Gavin AC, et al. (2002) CMR1 DNA2 ECM29 HDA1 HDA2 HDA3 HSC82 KAP95 LCD1 MEC1 MGM101 MLH1 MPH1 MRPS28 MSH2 MSH3 MSH6 OLA1 PGK1 PMS1 PRO2 RAD16 RAD23 RAD34 RAD4 RAD52 RFA1 RFA2 RFA3 RIM1 RNH1 RPN10 RPN12 RPN3 RPN6 RPN8 RPN9 RPT2 RPT3 RPT6 RSC8 RVB2 SGS1 SHS1 SRO7 SSL1 TFA1 TFB1 TFB4 TIF1, TIF2 TOP3 VAS1 VPS1 YHR122W
    View Details Qiu et al. (2008) DNA2 RRM3

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run CEP3 sample cbf3 from feb 2005 Sandall S, et all (2006)
    View Run RLF2 phosphorylation data - cascade search Green EM, et al (2005)
    View Run MLP2 #24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #12 Mitotic Prep1-TiO2 Flowthrough Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data DNA2 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..435] deduced N/A No confident structure predictions are available.
    2 View Details [436..754] MSA 2.021937 View MSA. No confident structure predictions are available.
    3 View Details [755..882]
    [970..1023]    		 PSI-BLAST 9.221849 RecG, N-terminal domain; RecG "wedge" domain; RecG helicase domain
    4 View Details [883..969]
    [1024..1231]    		 PSI-BLAST 9.221849 RecG, N-terminal domain; RecG "wedge" domain; RecG helicase domain
    5 View Details [1232..1423] PSI-BLAST 64.69897 DEXX box DNA helicase
    6 View Details [1424..1522] PSI-BLAST 2.39794 STRUCTURE OF DNA HELICASE WITH ADPNP


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.99

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle