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Protein Overview: PHO84

General Information:
Name(s) found: PHO84 / YML123C [SGD]
Description(s) found:
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  • gi|805019|emb|CAA89157.1| Pho84p [Saccharomyces cerevisiae] [NCBI NR]
  • gi|6323512|ref|NP_013583.1| High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; Pho84p [Sacch [NCBI NR]
  • gi|1346710|sp|P25297.2|PHO84_YEAST RecName: Full=Inorganic phosphate transporter PHO84 [NCBI NR]
  • pir||S54061 probable inorganic phosphate transport protein PHO84 - yeast (Saccharomyces cerevisiae) [NCBI NR]
  • Inorganic phosphate transporter PHO84 OS=Saccharomyces cerevisiae GN=PHO84 PE=1 SV=2 [Swiss-Prot]
  • (P25297) Inorganic phosphate transporter PHO84 [Swiss-Prot]
  • PHO84 Inorganic phosphate permease [mips]
  • High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p [SGD]
  • PHO84, Chr XIII from 24038-25801, reverse complement [yeast_orfs2]
  • Chr XIII from 24038-25801, reverse complement [yeastorf3.fasta]
  • Chr XIII from 25801-24038, reverse complement [yeastorf5.fasta]
  • PHO84 SGDID:S0004592, Chr XIII from 25801-24038, reverse complement, Verified ORF [yeastflysequest.fasta]
  • High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p [Saccharomyces cerevisiae]gi|805019|emb|C [nrdb_11152004_Chlamydomonas]
  • PHO84 SGDID:S000004592, Chr XIII from 25801-24038, reverse complement, Verified ORF, "High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit fr [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta]
  • Inorganic phosphate transporter PHO84 [nr-271106-contam.fasta]
  • Pho84p [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; Pho84p [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • PHO84 SGDID:S000004592, Chr XIII from 25801-24038, reverse complement, Verified ORF, "High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p" [yeast-bovrabprots.fasta]
  • [chemostatdb.fasta]
  • Inorganic phosphate transporter PHO84 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PHO84 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta]
  • PHO84 SGDID:S000004592, Chr XIII from 25800-24037, Genome Release 64-1-1, reverse complement, Verified ORF, "High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p" [yeast-030211-contam.fasta]
  • PHO84 SGDID:S000004592, Chr XIII from 25800-24037, Genome Release 64-2-1, reverse complement, Verified ORF, "High-affinity inorganic phosphate (Pi) transporter; also low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity" [20141208_orf_trans.fasta]
  • YML123C SGDID:S000004592, Chr XIII from 25800-24037, Genome Release 64-1-1, reverse complement, Verified ORF, "High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
    • Organism: Saccharomyces cerevisiae
    Length: 587 amino acids

    Gene Ontology:
    Cellular Component: integral to plasma membrane [IDALink to PubMed article][ISSLink to PubMed article]
    Biological Process: phosphate transport [IDALink to PubMed article][IMPLink to PubMed article][ISSLink to PubMed article]
    manganese ion transport [IMPLink to PubMed article]
    Molecular Function: inorganic phosphate transmembrane transporter activity [IDALink to PubMed article][IMPLink to PubMed article][ISSLink to PubMed article]
    manganese ion transmembrane transporter activity [IMPLink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSSVNKDTIH VAERSLHKEH LTEGGNMAFH NHLNDFAHIE DPLERRRLAL ESIDDEGFGW  60
   61 QQVKTISIAG VGFLTDSYDI FAINLGITMM SYVYWHGSMP GPSQTLLKVS TSVGTVIGQF 120
  121 GFGTLADIVG RKRIYGMELI IMIVCTILQT TVAHSPAINF VAVLTFYRIV MGIGIGGDYP 180
  181 LSSIITSEFA TTKWRGAIMG AVFANQAWGQ ISGGIIALIL VAAYKGELEY ANSGAECDAR 240
  241 CQKACDQMWR ILIGLGTVLG LACLYFRLTI PESPRYQLDV NAKLELAAAA QEQDGEKKIH 300
  301 DTSDEDMAIN GLERASTAVE SLDNHPPKAS FKDFCRHFGQ WKYGKILLGT AGSWFTLDVA 360
  361 FYGLSLNSAV ILQTIGYAGS KNVYKKLYDT AVGNLILICA GSLPGYWVSV FTVDIIGRKP 420
  421 IQLAGFIILT ALFCVIGFAY HKLGDHGLLA LYVICQFFQN FGPNTTTFIV PGECFPTRYR 480
  481 STAHGISAAS GKVGAIIAQT ALGTLIDHNC ARDGKPTNCW LPHVMEIFAL FMLLGIFTTL 540
  541 LIPETKRKTL EEINELYHDE IDPATLNFRN KNNDIESSSP SQLQHEA

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) FET4 PHO84
    View Details Ho Y, et al. (2002) AHA1 ARP2 ARP7 ART10 ATP3 BRX1 BUL1 BZZ1 CKB1 COX6 CPR6 DED1 DIG1 DIG2 DMA2 DUN1 ECM29 FAR10 FAR7 FAR8 FET4 GAL2 GAR1 GDE1 GFA1 GUS1 GYP5 HAS1 HOM6 HXT6 HXT7 IDH1 ILV5 KSS1 LAS17 LSB1 LSP1 LYS12 MET18 MKT1 MSE1 NAP1 NIP7 NMD3 NMD5 NOC2 NOP1 NOP2 NOP56 NOP7 NSA1 NSA2 NUG1 PEP1 PET10 PHO84 PIL1 PIM1 PMA1 PPH21 PPH22 PRB1 PRE10 PYC1 REP1 RGC1 RNQ1 RPA135 RPB3 RPB5 RPN1 RPN10 RPN12 RPN8 RPO21 RPO26 RRP1 RSP5 RVS167 SDH2 SEN1 SLA1 SPS1 SQT1 STE12 STE7 STH1 TEC1 UBI4 UBP7 URA7 VMA6 VMA8 VPS64 VPS74 YDR239C YHM2 YHR033W YKR018C YLR243W YPL009C
    View Details Qiu et al. (2008) ARR3 ATP6 ATP8 CTR3 ENB1 GAP1 OLI1 PHO84 PHO89 PMR1 SMF1 SSU1 SUL2 ZRT1

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run MCM16 No Comments Cheeseman IM, et al. (2002)
    View Run STU1 No Comments Cheeseman IM, et al. (2002)
    View Run TOR1 No Comments Reinke A, et al. (2004)
    View Run YNL313C No Comments Hazbun TR, et al. (2003)
    View Run TIM50 No Comments Hazbun TR, et al. (2003)
    View Run TOR1 No Comments Reinke A, et al. (2004)
    View Run YMR185W No Comments Hazbun TR, et al. (2003)
    View Run TIM50 No Comments Hazbun TR, et al. (2003)
    View Run PGA1 No Comments Hazbun TR, et al. (2003)
    View Run MIA40 No Comments Hazbun TR, et al. (2003)
    View Run GWT1 No Comments Hazbun TR, et al. (2003)
    View Run LST8 No Comments Reinke A, et al. (2004)
    View Run TOR2 No Comments Reinke A, et al. (2004)
    View Run HIR2 No Comments Green EM, et al (2005)
    View Run VAM3 sample: hx1 Hao Xu, et al. (2010)
    View Run VAM3 sample: hx2 Hao Xu, et al. (2010)
    View Run VAM3 sample: hx3 Hao Xu, et al. (2010)
    View Run VAM3 sample: hx4 Hao Xu, et al. (2010)
    View Run VAM3 Sample: HX11 - both fusion and trans-SNARE complex formation take place. Hao Xu, et al. (2010)
    View Run VAM3 Sample: HX12 - trans-SNARE complex formation and fusion are inhibited (control). Hao Xu, et al. (2010)
    View Run VAM3 Sample: HX13 - trans-SNARE complex forms but fusion is blocked. Hao Xu, et al. (2010)
    View Run VAM3 Sample: HX14 - mixture in detergent (control). Hao Xu, et al. (2010)
    View Run VAM3 hx21: both fusion and trans-SNARE complex formation take place. Investigating Vam3p, Nyv1p and their partners in trans-SNARE complex Hao Xu, et al. (2010)
    View Run VAM3 hx23: mixture in detergent (control) Investigating Vam3p, Nyv1p and their partners in trans-SNARE complex Hao Xu, et al. (2010)
    View Run MLP2 #19 Asynchronous Prep1-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #32 Asynchronous Prep-No Phosphopeptide enrichment Keck JM, et al. (2011)
    View Run MLP2 #19b Asynchronous Prep1-new search criteria Keck JM, et al. (2011)
    View Run MLP2 #19a Asynchronous Prep1-new search criteria Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    SHOWING SINGLE HITS. [ Hide Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data PHO84 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..271]
    [322..546]    		 PSI-BLAST 137.9897 No description for 1ja5A_ was found.
    2 View Details [272..321]
    [547..587]    		 PSI-BLAST 137.9897 No description for 1ja5A_ was found.

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • alcohol transmembrane transporter activity
    		•  6.7545566917184 bayes_pls_golite062009
  • polyol transmembrane transporter activity
    		•  6.7545566917184 bayes_pls_golite062009
  • glycerol transmembrane transporter activity
    		•  6.54968363274124 bayes_pls_golite062009
  • substrate-specific transmembrane transporter activity
    		•  5.46247398538901 bayes_pls_golite062009
  • glycerol-3-phosphate transmembrane transporter activity
    		•  5.40344495119017 bayes_pls_golite062009
  • ion transmembrane transporter activity
    		•  4.58978526526921 bayes_pls_golite062009
  • cation transmembrane transporter activity
    		•  4.31967711223954 bayes_pls_golite062009
  • transporter activity
    		•  4.09176719149278 bayes_pls_golite062009
  • anion transmembrane transporter activity
    		•  3.94605227012838 bayes_pls_golite062009
  • substrate-specific transporter activity
    		•  3.59744164024479 bayes_pls_golite062009
  • transmembrane transporter activity
    		•  3.5865018981083 bayes_pls_golite062009
  • organic anion transmembrane transporter activity
    		•  3.3413526350788 bayes_pls_golite062009
  • hexose phosphate transmembrane transporter activity
    		•  3.18173622457291 bayes_pls_golite062009
  • glucose-6-phosphate transmembrane transporter activity
    		•  3.18173622457291 bayes_pls_golite062009
  • cation:sugar symporter activity
    		•  2.99050613058546 bayes_pls_golite062009
  • sugar:hydrogen symporter activity
    		•  2.99050613058546 bayes_pls_golite062009
  • monosaccharide transmembrane transporter activity
    		•  2.63293544825311 bayes_pls_golite062009
  • carbohydrate transmembrane transporter activity
    		•  2.62141276633776 bayes_pls_golite062009
  • inorganic anion transmembrane transporter activity
    		•  2.52916479889426 bayes_pls_golite062009
  • sugar transmembrane transporter activity
    		•  2.47557586092261 bayes_pls_golite062009
  • organic acid transmembrane transporter activity
    		•  2.15783210522667 bayes_pls_golite062009
  • solute:hydrogen symporter activity
    		•  2.10485633822652 bayes_pls_golite062009
  • phosphate transmembrane transporter activity
    		•  1.78662033436109 bayes_pls_golite062009
  • active transmembrane transporter activity
    		•  1.59419818573076 bayes_pls_golite062009
  • binding
    		•  1.3181781780038 bayes_pls_golite062009
  • secondary active transmembrane transporter activity
    		•  1.22264637452409 bayes_pls_golite062009
  • solute:cation symporter activity
    		•  0.74591237387473 bayes_pls_golite062009
  • symporter activity
    		•  0.74591237387473 bayes_pls_golite062009
    2 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    [View Details]
    Source: Reynolds et al. 2008. Manuscript submitted Philius confidence legend
    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle