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Protein Overview: RSP5

General Information:
Name(s) found: RSP5 / YER125W [SGD]
Description(s) found:
Found 30 descriptions.
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  • E3 ubiquitin ligase of the NEDD4 family; involved in regulating many cellular processes, including MVB sorting, heat shock response, transcription, and endocytosis; the human homolog is invovled in Liddle syndrome [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 809 amino acids

    Gene Ontology:
    Cellular Component: ubiquitin ligase complex [IDALink to PubMed article]
    cytoplasm [IDALink to PubMed article]
    mitochondrion [IDALink to PubMed articleLink to PubMed article]
    Golgi apparatus [IDALink to PubMed article]
    endosome membrane [IDALink to PubMed article]
    plasma membrane [IDALink to PubMed article]
    Biological Process: mRNA export from nucleus [IMPLink to PubMed article]
    farnesyl diphosphate biosynthetic process, mevalonate pathway [IMPLink to PubMed article]
    transcription [TASLink to PubMed article]
    protein polyubiquitination [IDALink to PubMed article]
    chromatin assembly or disassembly [IMPLink to PubMed article]
    endocytosis [TASLink to PubMed article]
    protein monoubiquitination [IDALink to PubMed article]
    Molecular Function: ubiquitin-protein ligase activity [IDALink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MPSSISVKLV AAESLYKRDV FRSPDPFAVL TIDGYQTKST SAAKKTLNPY WNETFKFDDI  60
   61 NENSILTIQV FDQKKFKKKD QGFLGVVNVR VGDVLGHLDE DTATSSGRPR EETITRDLKK 120
  121 SNDGMAVSGR LIVVLSKLPS SSPHSQAPSG HTASSSTNTS STTRTNGHST SSTRNHSTSH 180
  181 PSRGTAQAVE STLQSGTTAA TNTATTSHRS TNSTSSATRQ YSSFEDQYGR LPPGWERRTD 240
  241 NFGRTYYVDH NTRTTTWKRP TLDQTEAERG NQLNANTELE RRQHRGRTLP GGSSDNSSVT 300
  301 VQVGGGSNIP PVNGAAAAAF AATGGTTSGL GELPSGWEQR FTPEGRAYFV DHNTRTTTWV 360
  361 DPRRQQYIRT YGPTNTTIQQ QPVSQLGPLP SGWEMRLTNT ARVYFVDHNT KTTTWDDPRL 420
  421 PSSLDQNVPQ YKRDFRRKVI YFRSQPALRI LPGQCHIKVR RKNIFEDAYQ EIMRQTPEDL 480
  481 KKRLMIKFDG EEGLDYGGVS REFFFLLSHE MFNPFYCLFE YSAYDNYTIQ INPNSGINPE 540
  541 HLNYFKFIGR VVGLGVFHRR FLDAFFVGAL YKMMLRKKVV LQDMEGVDAE VYNSLNWMLE 600
  601 NSIDGVLDLT FSADDERFGE VVTVDLKPDG RNIEVTDGNK KEYVELYTQW RIVDRVQEQF 660
  661 KAFMDGFNEL IPEDLVTVFD ERELELLIGG IAEIDIEDWK KHTDYRGYQE SDEVIQWFWK 720
  721 CVSEWDNEQR ARLLQFTTGT SRIPVNGFKD LQGSDGPRRF TIEKAGEVQQ LPKSHTCFNR 780
  781 VDLPQYVDYD SMKQKLTLAV EETIGFGQE

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) RSP5 RUP1 UBP2
    View Details Krogan NJ, et al. (2006) ADE6 BUL1 LDB19 RSP5 RUP1 UBP2 YHR131C
    View Details Ho Y, et al. (2002) ART10 BUL1 DUN1 HXT6 LSB1 MMS2 PHO84 RNQ1 RPB3 RPB5 RPO21 RPO26 RSP5 RVS161 RVS167 UBC13 YKR018C
    View Details Qiu et al. (2008) APC9 CDC16 RSP5

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run DAD1 Dam1-765 complex purified from yeast with Dad1-TAP Shimogawa MM, et al. (2006)
    View Run VAM3 Sample: HX12 - trans-SNARE complex formation and fusion are inhibited (control). Hao Xu, et al. (2010)
    View Run MLP2 #34 Asynchronous SPB prep Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    NOT SHOWING SINGLE HITS. [ Show Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data RSP5 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..199] PSI-BLAST 31.69897 Synaptogamin I
    2 View Details [200..314] PSI-BLAST 37.67837 Mitotic rotamase PIN1; Mitotic rotamase PIN1, domain 2
    3 View Details [315..372] PSI-BLAST 70.264245 Ubiquitin ligase NEDD4 WWIII domain
    4 View Details [373..450] ORFEUS 10.59 Ubiquitin ligase NEDD4 WWIII domain
    5 View Details [451..494] PSI-BLAST 664.0 Ubiquitin-protein ligase E3a, Hect catalytic domain (E6ap)
    6 View Details [495..592]
    [648..690]    		 PSI-BLAST 664.0 Ubiquitin-protein ligase E3a, Hect catalytic domain (E6ap)
    7 View Details [593..647]
    [691..809]    		 PSI-BLAST 664.0 Ubiquitin-protein ligase E3a, Hect catalytic domain (E6ap)

    Functions predicted (by domain):

    # Gene Ontology predictions
    1 No functions predicted.
    2
    Term Confidence Notes
  • ubiquitin-protein ligase activity
    		•  7.19063870734022 bayes_pls_golite062009
  • small conjugating protein ligase activity
    		•  6.90783262781184 bayes_pls_golite062009
  • acid-amino acid ligase activity
    		•  6.09904629433884 bayes_pls_golite062009
  • ligase activity, forming carbon-nitrogen bonds
    		•  5.1950291192201 bayes_pls_golite062009
  • ligase activity
    		•  4.32965319288714 bayes_pls_golite062009
  • transcription activator activity
    		•  3.61296179666474 bayes_pls_golite062009
  • transcription regulator activity
    		•  3.2682543222543 bayes_pls_golite062009
  • binding
    		•  2.62219069341981 bayes_pls_golite062009
  • protein binding
    		•  2.51948432647454 bayes_pls_golite062009
  • transcription factor binding
    		•  2.18630749239417 bayes_pls_golite062009
  • transcription cofactor activity
    		•  2.07432061489599 bayes_pls_golite062009
  • transcription coactivator activity
    		•  2.02433252968502 bayes_pls_golite062009
  • histone methyltransferase activity
    		•  1.90194027965368 bayes_pls_golite062009
  • protein-lysine N-methyltransferase activity
    		•  1.14806422628668 bayes_pls_golite062009
  • lysine N-methyltransferase activity
    		•  1.14806422628668 bayes_pls_golite062009
  • histone-lysine N-methyltransferase activity
    		•  1.14806422628668 bayes_pls_golite062009
  • protein methyltransferase activity
    		•  1.121001087082 bayes_pls_golite062009
  • catalytic activity
    		•  0.210712504691113 bayes_pls_golite062009
  • transcription repressor activity
    		•  0.18603158355229 bayes_pls_golite062009
  • histone methyltransferase activity (H3-K36 specific)
    		•  0.173723075520888 bayes_pls_golite062009
    3
    Term Confidence Notes
  • ubiquitin-protein ligase activity
    		•  7.19063870734022 bayes_pls_golite062009
  • small conjugating protein ligase activity
    		•  6.90783262781184 bayes_pls_golite062009
  • acid-amino acid ligase activity
    		•  6.09904629433884 bayes_pls_golite062009
  • ligase activity, forming carbon-nitrogen bonds
    		•  5.1950291192201 bayes_pls_golite062009
  • ligase activity
    		•  4.32965319288714 bayes_pls_golite062009
  • transcription activator activity
    		•  3.61296179666474 bayes_pls_golite062009
  • transcription regulator activity
    		•  3.2682543222543 bayes_pls_golite062009
  • binding
    		•  2.62219069341981 bayes_pls_golite062009
  • protein binding
    		•  2.51948432647454 bayes_pls_golite062009
  • transcription factor binding
    		•  2.18630749239417 bayes_pls_golite062009
  • transcription cofactor activity
    		•  2.07432061489599 bayes_pls_golite062009
  • transcription coactivator activity
    		•  2.02433252968502 bayes_pls_golite062009
  • histone methyltransferase activity
    		•  1.90194027965368 bayes_pls_golite062009
  • protein-lysine N-methyltransferase activity
    		•  1.14806422628668 bayes_pls_golite062009
  • lysine N-methyltransferase activity
    		•  1.14806422628668 bayes_pls_golite062009
  • histone-lysine N-methyltransferase activity
    		•  1.14806422628668 bayes_pls_golite062009
  • protein methyltransferase activity
    		•  1.121001087082 bayes_pls_golite062009
  • catalytic activity
    		•  0.210712504691113 bayes_pls_golite062009
  • transcription repressor activity
    		•  0.18603158355229 bayes_pls_golite062009
  • histone methyltransferase activity (H3-K36 specific)
    		•  0.173723075520888 bayes_pls_golite062009
    4
    Term Confidence Notes
  • ubiquitin-protein ligase activity
    		•  11.7414753295465 bayes_pls_golite062009
  • small conjugating protein ligase activity
    		•  11.4871643691645 bayes_pls_golite062009
  • acid-amino acid ligase activity
    		•  10.4323719338452 bayes_pls_golite062009
  • ligase activity, forming carbon-nitrogen bonds
    		•  9.13168684685485 bayes_pls_golite062009
  • ligase activity
    		•  7.74494807432593 bayes_pls_golite062009
  • sodium channel inhibitor activity
    		•  6.01425793895251 bayes_pls_golite062009
  • phosphothreonine binding
    		•  5.90497053948475 bayes_pls_golite062009
  • phosphoserine binding
    		•  5.90497053948475 bayes_pls_golite062009
  • ion channel inhibitor activity
    		•  2.86572445544473 bayes_pls_golite062009
  • channel inhibitor activity
    		•  2.86572445544473 bayes_pls_golite062009
  • binding
    		•  2.51893471705946 bayes_pls_golite062009
  • protein binding
    		•  2.44418607577971 bayes_pls_golite062009
  • transcription regulator activity
    		•  2.32249912875549 bayes_pls_golite062009
  • nucleic acid binding
    		•  2.12515000812201 bayes_pls_golite062009
  • DNA binding
    		•  1.96906809911381 bayes_pls_golite062009
  • SMAD binding
    		•  1.72092268696303 bayes_pls_golite062009
    		• 1.3844044305632 bayes_pls_golite062009
  • channel regulator activity
    		•  1.37005888814694 bayes_pls_golite062009
  • transcription activator activity
    		•  1.36023462068212 bayes_pls_golite062009
  • sodium channel regulator activity
    		•  1.19047973745862 bayes_pls_golite062009
  • receptor binding
    		•  1.15852292149378 bayes_pls_golite062009
  • catalytic activity
    		•  1.15152367676155 bayes_pls_golite062009
  • R-SMAD binding
    		•  1.09824752083864 bayes_pls_golite062009
  • activin binding
    		•  0.883990007327631 bayes_pls_golite062009
  • transcription factor binding
    		•  0.803487897091874 bayes_pls_golite062009
  • small conjugating protein binding
    		•  0.68109357273955 bayes_pls_golite062009
  • ubiquitin binding
    		•  0.60123113960943 bayes_pls_golite062009
  • ubiquitin-ubiquitin ligase activity
    		•  0.598436219078807 bayes_pls_golite062009
  • nuclear hormone receptor binding
    		•  0.470794254334721 bayes_pls_golite062009
    5 No functions predicted.
    6 No functions predicted.
    7 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.98

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle