Bait Protein: | VAM3 |
Organism: | Saccharomyces cerevisiae |
Publication: | Hao Xu , Youngsoo Jun, James Thompson, John Yates and William Wickner (2010) HOPS Prevents the Disassembly of Trans-SNARE Complexes by Sec17p/Sec18p during Membrane Fusion [In Press] |
Comments: | sample: hx1 |
Raw Data: |
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FILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
[ |
SEC17 | Peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' ste... | 13 | 37 | 44.5% | 0.0858 | 32803 |
[ |
NYV1 | v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent C... | 5 | 6 | 30.0% | 0.0161 | 28964 |
[ |
VAM7 | Component of the vacuole SNARE complex involved in vacuolar morphogenesis; SNAP-25 homolog; function... | 8 | 18 | 27.5% | 0.0386 | 36711 |
[ |
VTI1 | Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and ... | 5 | 13 | 26.7% | 0.0406 | 24668 |
[ |
RPP2A | Ribosomal protein P2 alpha, a component of the ribosomal stalk, which is involved in the interaction... | 2 | 2 | 24.5% | 0.0128 | 10746 |
[ |
RPP2B | Ribosomal protein P2 beta, a component of the ribosomal stalk, which is involved in the interaction ... | 2 | 2 | 23.6% | 0.0123 | 11050 |
[ |
VAM3 | Syntaxin-related protein required for vacuolar assembly; functions with Vam7p in vacuolar protein tr... | 5 | 15 | 18.4% | 0.0359 | 32498 |
[ |
PHO8 | Repressible alkaline phosphatase, a glycoprotein localized to the vacuole; regulated by levels of in... | 6 | 6 | 15.9% | 0.00718 | 63004 |
[ |
YKT6 | Vesicle membrane protein (v-SNARE) with acyltransferase activity; involved in trafficking to and wit... | 2 | 2 | 13.0% | 0.00677 | 22707 |
[ |
ROT1 | Molecular chaperone involved in protein folding in the ER; mutation causes defects in cell wall synt... | 2 | 2 | 12.1% | 0.00529 | 28908 |
[ |
CDC19 | Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... | 4 | 4 | 11.6% | 0.00542 | 54545 |
[ |
SHM2 | Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrof... | 3 | 3 | 8.7% | 0.00433 | 52219 |
[ |
PHO84 | High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated... | 3 | 3 | 8.0% | 0.00346 | 64382 |
[ |
VPH1 | Subunit a of vacuolar-ATPase V0 domain, one of two isoforms (Vph1p and Stv1p); Vph1p is located in V... | 4 | 4 | 7.3% | 0.00322 | 95529 |
[ |
PDA1 | E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex, catalyzes the direct oxidative decarbo... | 2 | 2 | 6.4% | 0.00322 | 46343 |
[ |
KAR2 | ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding i... | 2 | 2 | 4.5% | 0.00199 | 74468 |
[ |
VTC4 | Vacuolar membrane polyphosphate polymerase; subunit of the vacuolar transporter chaperone (VTC) comp... | 3 | 3 | 4.2% | 0.00282 | 83155 |
[ |
RGA1 | GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of se... | 2 | 2 | 4.0% | 0.00134 | 112831 |
[ |
VAM6 | Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by fa... | 2 | 2 | 3.3% | 0.00129 | 122881 |
[ |
YCF1 | Vacuolar glutathione S-conjugate transporter of the ATP-binding cassette family, has a role in detox... | 3 | 3 | 3.0% | 0.00134 | 171120 |