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Run Information:

Bait Protein: VAM3
Organism: Saccharomyces cerevisiae
Publication: Hao Xu , Youngsoo Jun, James Thompson, John Yates and William Wickner (2010) HOPS Prevents the Disassembly of Trans-SNARE Complexes by Sec17p/Sec18p during Membrane Fusion [In Press]
Comments: hx23: mixture in detergent (control) Investigating Vam3p, Nyv1p and their partners in trans-SNARE complex

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  Hit Protein Protein Desc Sequence Count Spectrum Count Sequence Coverage NSAF Mol. Wt.
[View Peptides]   PHO8 Repressible alkaline phosphatase, a glycoprotein localized to the vacuole; regulated by levels of in... 16 47 30.0% 0.00784 63004
[View Peptides]   VAM3 Syntaxin-related protein required for vacuolar assembly; functions with Vam7p in vacuolar protein tr... 7 27 26.5% 0.00901 32498
[View Peptides]   MAM3 Protein required for normal mitochondrial morphology, has similarity to hemolysins 9 26 17.7% 0.00348 77712
[View Peptides]   SEH1 Nuclear pore protein that is part of the evolutionarily conserved Nup84p complex (Nup84p, Nup85p, Nu... 4 4 17.5% 0.00108 39123
[View Peptides]   CDC19 Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... 6 11 15.8% 0.00208 54545
[View Peptides]   TSA1 Thioredoxin peroxidase, acts as both a ribosome-associated and free cytoplasmic antioxidant; self-as... 2 2 14.8% 9.64E-4 21590
[View Peptides]   NYV1 v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent C... 3 3 14.2% 0.00112 28964
[View Peptides]   VTI1 Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and ... 2 4 13.4% 0.00174 24668
[View Peptides]   YPK9 Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-t... 15 23 13.0% 0.00148 166749
[View Peptides]   YBL104C Putative protein of unknown function, promoter contains multiple GCN4 binding sites 8 10 12.0% 9.1E-4 117636
[View Peptides]   PPN1 Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer 6 9 11.9% 0.00126 78344
[View Peptides]   SRO9 Cytoplasmic RNA-binding protein that associates with translating ribosomes; involved in heme regulat... 3 4 11.8% 8.7E-4 48060
[View Peptides]   GPM1 Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycer... 2 2 10.9% 7.65E-4 27609
[View Peptides]   FRA1 Protein involved in negative regulation of transcription of iron regulon; forms an iron independent ... 6 8 10.4% 0.00101 84924
[View Peptides]   COT1 Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistanc... 3 3 10.0% 6.45E-4 48155
[View Peptides]   TEF1, TEF2 Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... 4 8 9.8% 0.00165 50033
[View Peptides]   PHO84 High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated... 4 7 9.4% 0.00113 64382
[View Peptides]   ACT1 Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functio... 3 6 8.8% 0.00151 41690
[View Peptides]   YNL217W Hypothetical protein 3 3 8.6% 8.69E-4 37151
[View Peptides]   UBI4 Ubiquitin, becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-... 2 13 6.6% 0.00322 42826
[View Peptides]   NPL3 RNA-binding protein that promotes elongation, regulates termination, and carries poly(A) mRNA from n... 2 2 6.5% 4.56E-4 45407
[View Peptides]   FBA1 Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversio... 2 3 6.4% 7.89E-4 39621
[View Peptides]   ZRC1 Vacuolar membrane zinc transporter, transports zinc from the cytosol into the vacuole for storage; a... 2 13 6.3% 0.00278 48345
[View Peptides]   VAM7 Component of the vacuole SNARE complex involved in vacuolar morphogenesis; SNAP-25 homolog; function... 2 2 6.3% 5.98E-4 36711
[View Peptides]   ADH1 Alcohol dehydrogenase, fermentative isozyme active as homo- or heterotetramers; required for the red... 2 2 6.0% 5.43E-4 36849
[View Peptides]   FAA4 Long chain fatty acyl-CoA synthetase, activates imported fatty acids with a preference for C12:0-C16... 3 4 5.8% 5.44E-4 77267
[View Peptides]   ULA1 Protein that acts together with Uba3p to activate Rub1p before its conjugation to proteins (neddylat... 2 2 5.8% 4.09E-4 52853
[View Peptides]   CPS1 Vacuolar carboxypeptidase yscS; expression is induced under low-nitrogen conditions 2 2 5.7% 3.28E-4 64597
[View Peptides]   ADE5,7 Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole... 3 3 4.6% 3.53E-4 86068
[View Peptides]   YIL108W Putative metalloprotease 2 2 4.6% 2.71E-4 77414
[View Peptides]   SLM1 Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in ... 2 2 4.5% 2.75E-4 77995
[View Peptides]   LRO1 Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that c... 2 3 4.4% 4.29E-4 75393
[View Peptides]   VAM6 Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by fa... 3 4 4.2% 3.6E-4 122881
[View Peptides]   MNR2 Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has si... 4 7 4.2% 6.82E-4 109736
[View Peptides]   YDR089W Protein of unknown function; deletion confers resistance to Nickel 2 3 3.7% 3.26E-4 98712
[View Peptides]   UTP25 Nucleolar protein that cosediments with the 40S ribosomal subunit and is required for its biogenesis... 2 2 3.7% 2.62E-4 83991
[View Peptides]   YCF1 Vacuolar glutathione S-conjugate transporter of the ATP-binding cassette family, has a role in detox... 4 6 3.4% 3.74E-4 171120
[View Peptides]   UBP3 Ubiquitin-specific protease that interacts with Bre5p to co-regulate anterograde and retrograde tran... 2 3 3.4% 3.11E-4 101917
[View Peptides]   MNN1 Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for add... 2 2 3.3% 2.48E-4 88529
[View Peptides]   PHO90 Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthet... 2 2 3.2% 2.14E-4 97689
[View Peptides]   VPH1 Subunit a of vacuolar-ATPase V0 domain, one of two isoforms (Vph1p and Stv1p); Vph1p is located in V... 2 2 3.1% 2.25E-4 95529
[View Peptides]   VPS41 Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sort... 2 2 3.0% 1.9E-4 113412
[View Peptides]   VAC7 Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p k... 2 3 2.7% 2.43E-4 128140
[View Peptides]   PHO91 Low-affinity phosphate transporter of the vacuolar membrane; deletion of pho84, pho87, pho89, pho90,... 2 3 2.5% 3.17E-4 99490
[View Peptides]   URA2 Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes t... 3 3 2.1% 1.28E-4 245123
[View Peptides]   HSL1 Nim1p-related protein kinase that regulates the morphogenesis and septin checkpoints; associates wit... 2 6 1.2% 3.73E-4 169592

YRC Informatics Platform - Version 3.0
Created and Maintained by: Michael Riffle