Bait Protein: | VAM3 |
Organism: | Saccharomyces cerevisiae |
Publication: | Hao Xu , Youngsoo Jun, James Thompson, John Yates and William Wickner (2010) HOPS Prevents the Disassembly of Trans-SNARE Complexes by Sec17p/Sec18p during Membrane Fusion [In Press] |
Comments: | hx23: mixture in detergent (control) Investigating Vam3p, Nyv1p and their partners in trans-SNARE complex |
Raw Data: |
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FILTERED Run Results:
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Hit Protein | Protein Desc | Sequence Count | Spectrum Count | Sequence Coverage | NSAF | Mol. Wt. | |
[ |
PHO8 | Repressible alkaline phosphatase, a glycoprotein localized to the vacuole; regulated by levels of in... | 16 | 47 | 30.0% | 0.00784 | 63004 |
[ |
VAM3 | Syntaxin-related protein required for vacuolar assembly; functions with Vam7p in vacuolar protein tr... | 7 | 27 | 26.5% | 0.00901 | 32498 |
[ |
MAM3 | Protein required for normal mitochondrial morphology, has similarity to hemolysins | 9 | 26 | 17.7% | 0.00348 | 77712 |
[ |
SEH1 | Nuclear pore protein that is part of the evolutionarily conserved Nup84p complex (Nup84p, Nup85p, Nu... | 4 | 4 | 17.5% | 0.00108 | 39123 |
[ |
CDC19 | Pyruvate kinase, functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvat... | 6 | 11 | 15.8% | 0.00208 | 54545 |
[ |
TSA1 | Thioredoxin peroxidase, acts as both a ribosome-associated and free cytoplasmic antioxidant; self-as... | 2 | 2 | 14.8% | 9.64E-4 | 21590 |
[ |
NYV1 | v-SNARE component of the vacuolar SNARE complex involved in vesicle fusion; inhibits ATP-dependent C... | 3 | 3 | 14.2% | 0.00112 | 28964 |
[ |
VTI1 | Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and ... | 2 | 4 | 13.4% | 0.00174 | 24668 |
[ |
YPK9 | Vacuolar protein with a possible role in sequestering heavy metals; has similarity to the type V P-t... | 15 | 23 | 13.0% | 0.00148 | 166749 |
[ |
YBL104C | Putative protein of unknown function, promoter contains multiple GCN4 binding sites | 8 | 10 | 12.0% | 9.1E-4 | 117636 |
[ |
PPN1 | Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer | 6 | 9 | 11.9% | 0.00126 | 78344 |
[ |
SRO9 | Cytoplasmic RNA-binding protein that associates with translating ribosomes; involved in heme regulat... | 3 | 4 | 11.8% | 8.7E-4 | 48060 |
[ |
GPM1 | Tetrameric phosphoglycerate mutase, mediates the conversion of 3-phosphoglycerate to 2-phosphoglycer... | 2 | 2 | 10.9% | 7.65E-4 | 27609 |
[ |
FRA1 | Protein involved in negative regulation of transcription of iron regulon; forms an iron independent ... | 6 | 8 | 10.4% | 0.00101 | 84924 |
[ |
COT1 | Vacuolar transporter that mediates zinc transport into the vacuole; overexpression confers resistanc... | 3 | 3 | 10.0% | 6.45E-4 | 48155 |
[ |
TEF1, TEF2 | Translational elongation factor EF-1 alpha; also encoded by TEF2; functions in the binding reaction ... | 4 | 8 | 9.8% | 0.00165 | 50033 |
[ |
PHO84 | High-affinity inorganic phosphate (Pi) transporter and low-affinity manganese transporter; regulated... | 4 | 7 | 9.4% | 0.00113 | 64382 |
[ |
ACT1 | Actin, structural protein involved in cell polarization, endocytosis, and other cytoskeletal functio... | 3 | 6 | 8.8% | 0.00151 | 41690 |
[ |
YNL217W | Hypothetical protein | 3 | 3 | 8.6% | 8.69E-4 | 37151 |
[ |
UBI4 | Ubiquitin, becomes conjugated to proteins, marking them for selective degradation via the ubiquitin-... | 2 | 13 | 6.6% | 0.00322 | 42826 |
[ |
NPL3 | RNA-binding protein that promotes elongation, regulates termination, and carries poly(A) mRNA from n... | 2 | 2 | 6.5% | 4.56E-4 | 45407 |
[ |
FBA1 | Fructose 1,6-bisphosphate aldolase, required for glycolysis and gluconeogenesis; catalyzes conversio... | 2 | 3 | 6.4% | 7.89E-4 | 39621 |
[ |
ZRC1 | Vacuolar membrane zinc transporter, transports zinc from the cytosol into the vacuole for storage; a... | 2 | 13 | 6.3% | 0.00278 | 48345 |
[ |
VAM7 | Component of the vacuole SNARE complex involved in vacuolar morphogenesis; SNAP-25 homolog; function... | 2 | 2 | 6.3% | 5.98E-4 | 36711 |
[ |
ADH1 | Alcohol dehydrogenase, fermentative isozyme active as homo- or heterotetramers; required for the red... | 2 | 2 | 6.0% | 5.43E-4 | 36849 |
[ |
FAA4 | Long chain fatty acyl-CoA synthetase, activates imported fatty acids with a preference for C12:0-C16... | 3 | 4 | 5.8% | 5.44E-4 | 77267 |
[ |
ULA1 | Protein that acts together with Uba3p to activate Rub1p before its conjugation to proteins (neddylat... | 2 | 2 | 5.8% | 4.09E-4 | 52853 |
[ |
CPS1 | Vacuolar carboxypeptidase yscS; expression is induced under low-nitrogen conditions | 2 | 2 | 5.7% | 3.28E-4 | 64597 |
[ |
ADE5,7 | Bifunctional enzyme of the 'de novo' purine nucleotide biosynthetic pathway, contains aminoimidazole... | 3 | 3 | 4.6% | 3.53E-4 | 86068 |
[ |
YIL108W | Putative metalloprotease | 2 | 2 | 4.6% | 2.71E-4 | 77414 |
[ |
SLM1 | Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in ... | 2 | 2 | 4.5% | 2.75E-4 | 77995 |
[ |
LRO1 | Acyltransferase that catalyzes diacylglycerol esterification; one of several acyltransferases that c... | 2 | 3 | 4.4% | 4.29E-4 | 75393 |
[ |
VAM6 | Vacuolar protein that plays a critical role in the tethering steps of vacuolar membrane fusion by fa... | 3 | 4 | 4.2% | 3.6E-4 | 122881 |
[ |
MNR2 | Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has si... | 4 | 7 | 4.2% | 6.82E-4 | 109736 |
[ |
YDR089W | Protein of unknown function; deletion confers resistance to Nickel | 2 | 3 | 3.7% | 3.26E-4 | 98712 |
[ |
UTP25 | Nucleolar protein that cosediments with the 40S ribosomal subunit and is required for its biogenesis... | 2 | 2 | 3.7% | 2.62E-4 | 83991 |
[ |
YCF1 | Vacuolar glutathione S-conjugate transporter of the ATP-binding cassette family, has a role in detox... | 4 | 6 | 3.4% | 3.74E-4 | 171120 |
[ |
UBP3 | Ubiquitin-specific protease that interacts with Bre5p to co-regulate anterograde and retrograde tran... | 2 | 3 | 3.4% | 3.11E-4 | 101917 |
[ |
MNN1 | Alpha-1,3-mannosyltransferase, integral membrane glycoprotein of the Golgi complex, required for add... | 2 | 2 | 3.3% | 2.48E-4 | 88529 |
[ |
PHO90 | Low-affinity phosphate transporter; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthet... | 2 | 2 | 3.2% | 2.14E-4 | 97689 |
[ |
VPH1 | Subunit a of vacuolar-ATPase V0 domain, one of two isoforms (Vph1p and Stv1p); Vph1p is located in V... | 2 | 2 | 3.1% | 2.25E-4 | 95529 |
[ |
VPS41 | Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sort... | 2 | 2 | 3.0% | 1.9E-4 | 113412 |
[ |
VAC7 | Integral vacuolar membrane protein involved in vacuole inheritance and morphology; activates Fab1p k... | 2 | 3 | 2.7% | 2.43E-4 | 128140 |
[ |
PHO91 | Low-affinity phosphate transporter of the vacuolar membrane; deletion of pho84, pho87, pho89, pho90,... | 2 | 3 | 2.5% | 3.17E-4 | 99490 |
[ |
URA2 | Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase), catalyzes t... | 3 | 3 | 2.1% | 1.28E-4 | 245123 |
[ |
HSL1 | Nim1p-related protein kinase that regulates the morphogenesis and septin checkpoints; associates wit... | 2 | 6 | 1.2% | 3.73E-4 | 169592 |