Protein Overview: PMA1

General Information:
Name(s) found:	PMA1 / YGL008C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6321430\|ref\|NP_011507.1\| Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; part of the P2 subgroup of cation-transporting ATPases; Pma1p [Saccharomyces cerevisiae] [NCBI NR] gi\|4187\|emb\|CAA27237.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] pir\|\|PXBY1P H+-exporting ATPase (EC 3.6.3.6) 1, plasma membrane - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1322461\|emb\|CAA96708.1\| PMA1 [Saccharomyces cerevisiae] [NCBI NR] gi\|1168544\|sp\|P05030.2\|PMA1_YEAST RecName: Full=Plasma membrane ATPase 1; AltName: Full=Proton pump 1 [NCBI NR] Plasma membrane ATPase 1 OS=Saccharomyces cerevisiae GN=PMA1 PE=1 SV=2 [Swiss-Prot] PMA1 H+-transporting P-type ATPase [mips] Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; part of the P2 subgroup of cation-transporting ATPases [SGD] PMA1, Chr VII from 479906-482662, reverse complement [yeast_orfs2] Chr VII from 479906-482662, reverse complement [yeastorf3.fasta] Chr VII from 479913-482669, reverse complement [yeastorf4.fasta] Chr VII from 482669-479913, reverse complement [yeastorf5.fasta] (P05030) Plasma membrane ATPase 1 (EC 3.6.3.6) (Proton pump 1) [4932.FASTACCERV.txt] PMA1 SGDID:S0002976, Chr VII from 482669-479913, reverse complement, Verified ORF [yeastflysequest.fasta] PMA1 SGDID:S0002976, Chr VII from 482671-479915, reverse complement, Verified ORF [10112005cervSGD.fasta] Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH; part of the P2 subgroup of cation-transporting ATPases [Saccharomyces cerevisiae]gi\|4187\|emb\|CAA27237.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|13 [nrdb_11152004_Chlamydomonas] PMA1 SGDID:S000002976, Chr VII from 482671-479915, reverse complement, Verified ORF, "Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; part of the P2 subgroup of cation-transporting [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Plasma membrane ATPase 1 (Proton pump 1) [nr-271106-contam.fasta] PMA1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; part of the P2 subgroup of cation-transporting ATPases; Pma1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] PMA1 SGDID:S000002976, Chr VII from 482671-479915, reverse complement, Verified ORF, "Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; part of the P2 subgroup of cation-transporting ATPases" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Plasma membrane ATPase 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PMA1 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] PMA1 SGDID:S000002976, Chr VII from 482666-479910, Genome Release 64-1-1, reverse complement, Verified ORF, "Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; P2-type ATPase; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+]" [yeast-030211-contam.fasta] PMA1 SGDID:S000002976, Chr VII from 482666-479910, Genome Release 64-2-1, reverse complement, Verified ORF, "Plasma membrane P2-type H+-ATPase; pumps protons out of cell; major regulator of cytoplasmic pH and plasma membrane potential; long-lived protein asymmetrically distributed at plasma membrane between mother cells and buds; accumulates at high levels in mother cells during aging, buds emerge with very low levels of Pma1p, newborn cells have low levels of Pma1p; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+]" [20141208_orf_trans.fasta] YGL008C SGDID:S000002976, Chr VII from 482666-479910, Genome Release 64-1-1, reverse complement, Verified ORF, "Plasma membrane H+-ATPase, pumps protons out of the cell; major regulator of cytoplasmic pH and plasma membrane potential; P2-type ATPase; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+]" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	918 amino acids
Gene Ontology:
Cellular Component:	mitochondrion [IDA] membrane raft [IDA] plasma membrane [IDA ]
Biological Process:	proton transport [IDA] regulation of pH [IEP]
Molecular Function:	hydrogen-exporting ATPase activity, phosphorylative mechanism [TAS ]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MTDTSSSSSS SSASSVSAHQ PTQEKPAKTY DDAASESSDD DDIDALIEEL QSNHGVDDED  60
   61 SDNDGPVAAG EARPVPEEYL QTDPSYGLTS DEVLKRRKKY GLNQMADEKE SLVVKFVMFF 120
  121 VGPIQFVMEA AAILAAGLSD WVDFGVICGL LMLNAGVGFV QEFQAGSIVD ELKKTLANTA 180
  181 VVIRDGQLVE IPANEVVPGD ILQLEDGTVI PTDGRIVTED CFLQIDQSAI TGESLAVDKH 240
  241 YGDQTFSSST VKRGEGFMVV TATGDNTFVG RAAALVNKAA GGQGHFTEVL NGIGIILLVL 300
  301 VIATLLLVWT ACFYRTNGIV RILRYTLGIT IIGVPVGLPA VVTTTMAVGA AYLAKKQAIV 360
  361 QKLSAIESLA GVEILCSDKT GTLTKNKLSL HEPYTVEGVS PDDLMLTACL AASRKKKGLD 420
  421 AIDKAFLKSL KQYPKAKDAL TKYKVLEFHP FDPVSKKVTA VVESPEGERI VCVKGAPLFV 480
  481 LKTVEEDHPI PEDVHENYEN KVAELASRGF RALGVARKRG EGHWEILGVM PCMDPPRDDT 540
  541 AQTVSEARHL GLRVKMLTGD AVGIAKETCR QLGLGTNIYN AERLGLGGGG DMPGSELADF 600
  601 VENADGFAEV FPQHKYRVVE ILQNRGYLVA MTGDGVNDAP SLKKADTGIA VEGATDAARS 660
  661 AADIVFLAPG LSAIIDALKT SRQIFHRMYS YVVYRIALSL HLEIFLGLWI AILDNSLDID 720
  721 LIVFIAIFAD VATLAIAYDN APYSPKPVKW NLPRLWGMSI ILGIVLAIGS WITLTTMFLP 780
  781 KGGIIQNFGA MNGIMFLQIS LTENWLIFIT RAAGPFWSSI PSWQLAGAVF AVDIIATMFT 840
  841 LFGWWSENWT DIVTVVRVWI WSIGIFCVLG GFYYEMSTSE AFDRLMNGKP MKEKKSTRSV 900
  901 EDFMAAMQRV STQHEKET

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		PMA1 POR1 RAD4 RPL20A RPL26A RPL2A, RPL2B RPL3 RPL4A RPL9A RPS14A
View Details	(MIPS) Mewes HW, et al. (2004)		PMA1 PMA2 PMP1 PMP2
View Details	Krogan NJ, et al. (2006)		GPD1 PDR5 PHO86 PMA1 PMT1 SSH1 WBP1 YGR266W
View Details	Ho Y, et al. (2002)		AHA1 ARP2 ARP7 BCK1 CDC33 CKA1 CKA2 CKB1 CPR6 DAP1 DIG1 DIG2 EGD2 ESF1 ESF2 FET4 FOL2 GAL7 GCN1 GFA1 GLC7 GND1 GSP1 GSP2 GUF1 GUS1 HAS1 HTB1 HXT6 IDH1 ILV5 IMD3 IPP1 KIC1 KIN2 KOG1 KSS1 LAA1 LHP1 LHS1 LYS12 MKK2 MKT1 MSE1 MSS116 NAP1 NET1 NMD5 NOP12 NOP6 NSR1 NUS1 OYE2 PDC6 PHO84 PIL1 PIM1 PMA1 PMA2 PPZ2 PYC1 QCR2 RCN2 REG1 RGC1 RPA135 RPF2 RPN10 RPN6 RPT3 RSE1 RVB1 SDS22 SEN1 SIS1 SKG3 SLT2 SMK1 SNF4 STE12 STE7 TEC1 TIF1, TIF2 UBI4 YCR087W YDR102C YDR239C YGR130C YHR033W YRA1
View Details	Qiu et al. (2008)		PMA1 PMA2 TRK1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	TOR1	No Comments	Reinke A, et al. (2004)
View Run	BIR1	No Comments	Widlund PO, et al. (2005)
View Run	TOR1	No Comments	Reinke A, et al. (2004)
View Run	TOR2	No Comments	Reinke A, et al. (2004)
View Run	LST8	No Comments	Reinke A, et al. (2004)
View Run	TOR2	No Comments	Reinke A, et al. (2004)
View Run	KOG1	No Comments	Reinke A, et al. (2004)
View Run	DAD1	Dam1 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	RLF2	cac1: TAP-tagged	Green EM, et al (2005)
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	DAD1	Dam1 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	RLF2	phosphorylation data - cascade search	Green EM, et al (2005)
View Run	RLF2	identification data - first search in cascade	Green EM, et al (2005)
View Run	ASF1	sample 3928 - Asf1-S-TEV-ZZ (in hir3delta mutant)	Green EM, et al (2005)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	BOI1	Sample boi 1 with ha tag from october 2005	McCusker D, et al (2007)
View Run	BOI1	Sample boi1 with gst from october 2005	McCusker D, et al (2007)
View Run	VAM3	Sample: HX11 - both fusion and trans-SNARE complex formation take place.	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX12 - trans-SNARE complex formation and fusion are inhibited (control).	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX14 - mixture in detergent (control).	Hao Xu, et al. (2010)
View Run	MLP2	#19 Asynchronous Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#25 Asynchronous Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2	Keck JM, et al. (2011)
View Run	MLP2	#22 Asynchronous Prep2-IMAC Phosphopeptide enrichment B1-2	Keck JM, et al. (2011)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#04 Alpha Factor Prep2	Keck JM, et al. (2011)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#10 Mitotic Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#02 Alpha Factor Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#01 Alpha factor Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#28 Asynchronous Prep5-TiO2 Phosphopeptide Enrichment	Keck JM, et al. (2011)
View Run	MLP2	#06 Alpha Factor Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#08 Alpha Factor Prep4-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#19b Asynchronous Prep1-new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#05 Alpha Factor Prep2-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#07 Alpha Factor Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#09 Alpha Factor Prep4-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#19a Asynchronous Prep1-new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	PMA1	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..107] [178..280]	PSI-BLAST	1306.9897	Calcium ATPase, transduction domain A; Calcium ATPase, catalytic domain P; Calcium ATPase; Calcium ATPase, transmembrane domain M
2	View Details	[108..167] [281..345] [684..918]	PSI-BLAST	1306.9897	Calcium ATPase, transduction domain A; Calcium ATPase, catalytic domain P; Calcium ATPase; Calcium ATPase, transmembrane domain M
3	View Details	[168..177] [346..385] [536..683]	PSI-BLAST	1306.9897	Calcium ATPase, transduction domain A; Calcium ATPase, catalytic domain P; Calcium ATPase; Calcium ATPase, transmembrane domain M
4	View Details	[386..535]	PSI-BLAST	1306.9897	Calcium ATPase, transduction domain A; Calcium ATPase, catalytic domain P; Calcium ATPase; Calcium ATPase, transmembrane domain M

Protein Overview: PMA1

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: