Protein Overview: SEC53

General Information:
Name(s) found:	SEC53 / YFL045C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|836710\|dbj\|BAA09196.1\| phosphomannomutase [Saccharomyces cerevisiae] [NCBI NR] pir\|\|BVBY53 phosphomannomutase (EC 5.4.2.8) - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|51013369\|gb\|AAT92978.1\| YFL045C [Saccharomyces cerevisiae] [NCBI NR] gi\|45720074\|emb\|CAE52255.1\| Sec53p [Saccharomyces cerevisiae] [NCBI NR] gi\|45720072\|emb\|CAE52254.1\| Sec53p [Saccharomyces cerevisiae] [NCBI NR] gi\|45720070\|emb\|CAE52253.1\| Sec53p [Saccharomyces cerevisiae] [NCBI NR] gi\|45720068\|emb\|CAE52252.1\| Sec53p [Saccharomyces cerevisiae] [NCBI NR] gi\|45720064\|emb\|CAE52250.1\| Sec53p [Saccharomyces cerevisiae] [NCBI NR] gi\|45720062\|emb\|CAE52249.1\| Sec53p [Saccharomyces cerevisiae] [NCBI NR] gi\|45720058\|emb\|CAE52247.1\| Sec53p [Saccharomyces cerevisiae] [NCBI NR] gi\|45720056\|emb\|CAE52246.1\| Sec53p [Saccharomyces cerevisiae] [NCBI NR] gi\|45720054\|emb\|CAE52245.1\| Sec53p [Saccharomyces cerevisiae] [NCBI NR] gi\|45720052\|emb\|CAE52244.1\| Sec53p [Saccharomyces cerevisiae] [NCBI NR] gi\|45720050\|emb\|CAE52243.1\| Sec53p [Saccharomyces cerevisiae] [NCBI NR] gi\|45720048\|emb\|CAE52242.1\| Sec53p [Saccharomyces cerevisiae] [NCBI NR] gi\|45720046\|emb\|CAE52241.1\| Sec53p [Saccharomyces cerevisiae] [NCBI NR] gi\|45720044\|emb\|CAE52240.1\| SEC53p [Saccharomyces cerevisiae] [NCBI NR] gi\|4445\|emb\|CAA26957.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|14318474\|ref\|NP_116609.1\| Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen; Sec53p [Saccharomyces cerevisiae] [NCBI NR] gi\|134285\|sp\|P07283.1\|PMM_YEAST RecName: Full=Phosphomannomutase; Short=PMM [NCBI NR] Phosphomannomutase OS=Saccharomyces cerevisiae GN=SEC53 PE=1 SV=1 [Swiss-Prot] SEC53 Phosphomannomutase [mips] Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen [SGD] SEC53, Chr VI from 43628-44392, reverse complement [yeast_orfs2] Chr VI from 43628-44392, reverse complement [yeastorf3.fasta] Chr VI from 44392-43628, reverse complement [yeastorf5.fasta] (P07283) Phosphomannomutase (EC 5.4.2.8) (PMM) [4932.FASTACCERV.txt] SEC53 SGDID:S0001849, Chr VI from 44392-43628, reverse complement, Verified ORF [yeastflysequest.fasta] Sec53p [Saccharomyces cerevisiae]gi\|51013369\|gb\|AAT92978.1\| YFL045C [Saccharomyces cerevisiae]gi\|4445\|emb\|CAA26957.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|134285\|sp\|P07283\|PMM_YEAST Phosphomannomutase (PMM)gi\|73085\|pir\|\|BVBY53 phosphom [nrdb_11152004_Chlamydomonas] SEC53 SGDID:S000001849, Chr VI from 44392-43628, reverse complement, Verified ORF, "Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Sec53p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Phosphomannomutase (PMM) [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] phosphomannomutase [Saccharomyces cerevisiae] [nr-271106-contam.fasta] SEC53p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] YFL045C [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen; Sec53p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] Phosphomannomutase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SEC53 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] SEC53 SGDID:S000001849, Chr VI from 44392-43628, Genome Release 64-1-1, reverse complement, Verified ORF, "Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen" [yeast-030211-contam.fasta] SEC53 SGDID:S000001849, Chr VI from 44392-43628, Genome Release 64-2-1, reverse complement, Verified ORF, "Phosphomannomutase; involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen" [20141208_orf_trans.fasta] TR6246\|c0_g1_i1\|g.4386 ORF TR6246\|c0_g1_i1\|g.4386 TR6246\|c0_g1_i1\|m.4386 type:complete len:255 (+) TR6246\|c0_g1_i1:82-846(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] TR6465\|c0_g1_i1\|g.4650 ORF TR6465\|c0_g1_i1\|g.4650 TR6465\|c0_g1_i1\|m.4650 type:complete len:255 (+) TR6465\|c0_g1_i1:139-903(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YFL045C SGDID:S000001849, Chr VI from 44392-43628, Genome Release 64-1-1, reverse complement, Verified ORF, "Phosphomannomutase, involved in synthesis of GDP-mannose and dolichol-phosphate-mannose; required for folding and glycosylation of secretory proteins in the ER lumen" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_9490\|g.6872 ORF asmbl_9490\|g.6872 asmbl_9490\|m.6872 type:complete len:255 (+) asmbl_9490:76-840(+) [DBVPG6044_Trinity_v2_2_0_PASA_6May2016-contam.fasta] asmbl_9443\|g.6874 ORF asmbl_9443\|g.6874 asmbl_9443\|m.6874 type:complete len:255 (+) asmbl_9443:76-840(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_9343\|g.5751 ORF ID=asmbl_9343\|g.5751 ID=asmbl_9343\|m.5751 type:complete len:255 (+) ID=asmbl_9343:76-840(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta] ID=asmbl_9450\|g.5670 ORF ID=asmbl_9450\|g.5670 ID=asmbl_9450\|m.5670 type:complete len:255 (+) ID=asmbl_9450:76-840(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	254 amino acids
Gene Ontology:
Cellular Component:	cytosol [IDA]
Biological Process:	protein targeting to ER [IMP]
Molecular Function:	phosphomannomutase activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSIAEFAYKE KPETLVLFDV DGTLTPARLT VSEEVRKTLA KLRNKCCIGF VGGSDLSKQL  60
   61 EQLGPNVLDE FDYSFSENGL TAYRLGKELA SQSFINWLGE EKYNKLAVFI LRYLSEIDLP 120
  121 KRRGTFLEFR NGMINVSPIG RNASTEERNE FERYDKEHQI RAKFVEALKK EFPDYGLTFS 180
  181 IGGQISFDVF PAGWDKTYCL QHVEKDGFKE IHFFGDKTMV GGNDYEIFVD ERTIGHSVQS 240
  241 PDDTVKILTE LFNL

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		OLA1 SEC53
View Details	Gavin AC, et al. (2006)		FUR1 PMR1 RVB2 SEC53
View Details	Ho Y, et al. (2002)		AAC3 AAT2 ACC1 ACH1 ACO1 ADE17 ADH4 ADH6 ADK1 AFR1 AHA1 AHP1 APM4 ARC1 ARC35 ARF1 ATP3 BEM2 BIO3 BLM10 CAR2 CCT6 CDC12 CDC14 CDC15 CDC33 CLU1 COF1 COR1 CPR1 CPR3 CPR6 CYR1 CYS4 DOG1 DOG2 DUG2 DUN1 DUT1 ECM10 EFB1 EGD1 EGD2 ERB1 ERG13 ERG20 ERG6 FAA4 FPR1 FRS2 GAL83 GCD11 GDI1 GND1 GND2 GRX1 GUS1 HAS1 HEF3 HHF1, HHF2 HOM3 HOR2 HXT7 IDH2 ILV2 IMD4 KGD1 LIA1 LRO1 LSP1 MAM33 MDH1 MET6 MRT4 MSK1 MUB1 NOC2 NOC3 NOG1 NSA1 NTF2 NUG1 OLA1 OYE2 PFK1 PGM2 PIL1 PRB1 PRM2 PRP43 PTC3 PTC7 PYC1 QCR2 RAD26 RAD3 RAD52 RAD59 RGD1 RHR2 RNR2 RPT4 RSN1 SAH1 SCP160 SCS2 SEC23 SEC27 SEC53 SEN15 SES1 SGD1 SIP2 SIR2 SIR3 SIR4 SNF1 SNF4 SNU13 SOD1 SPB4 SRP1 SRS2 SSQ1 STE4 TCP1 TEF4 TEM1 TFP1 THS1 TIF1, TIF2 TIF6 TMA19 TMA29 TSA2 UBA1 UBR2 UTP5 VMA22 VMA4 VMA5 VMA8 WTM1 YER138C YGR066C YHB1 YNK1 YPT31 YSP2 YTM1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	YIF1	SEC53	1	Unpublished Fields Lab Data
View Screen	CAB2	SEC53	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	SEC53	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..254]	Pfam	179.236572	Eukaryotic phosphomannomutase No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
hydrolase activity	1.86932838801412	bayes_pls_golite062009
phosphatase activity	1.65156839204136	bayes_pls_golite062009
catalytic activity	1.55296860681783	bayes_pls_golite062009
phosphoric ester hydrolase activity	1.45258865666631	bayes_pls_golite062009
ATPase activity, coupled to transmembrane movement of ions, phosphorylative mechanism	1.33388154652051	bayes_pls_golite062009
substrate-specific transporter activity	1.31348938920819	bayes_pls_golite062009
substrate-specific transmembrane transporter activity	1.22591357588429	bayes_pls_golite062009
ion transmembrane transporter activity	1.15184224078831	bayes_pls_golite062009
ATPase activity, coupled to transmembrane movement of ions	1.07982919239634	bayes_pls_golite062009
di-, tri-valent inorganic cation transmembrane transporter activity	0.912974008714912	bayes_pls_golite062009
nucleic acid binding	0.911070095136259	bayes_pls_golite062009
binding	0.89911057995199	bayes_pls_golite062009
cation transmembrane transporter activity	0.843430522659521	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.764404472034968	bayes_pls_golite062009
pyrophosphatase activity	0.715824902222533	bayes_pls_golite062009
ATPase activity	0.714770840126679	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	0.694955044437904	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	0.693893836676894	bayes_pls_golite062009
hydrolase activity, acting on ester bonds	0.637441292083802	bayes_pls_golite062009
metal ion transmembrane transporter activity	0.527631061477265	bayes_pls_golite062009
inorganic cation transmembrane transporter activity	0.454331447449945	bayes_pls_golite062009
DNA binding	0.452491037562571	bayes_pls_golite062009
ATPase activity, coupled	0.287341673798449	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	0.197599118916494	bayes_pls_golite062009
active transmembrane transporter activity	0.194344891491143	bayes_pls_golite062009
cation-transporting ATPase activity	0.15490458026932	bayes_pls_golite062009
kinase activity	0.126534028294514	bayes_pls_golite062009
transporter activity	0.1049846678839	bayes_pls_golite062009
primary active transmembrane transporter activity	0.053543166906854	bayes_pls_golite062009
P-P-bond-hydrolysis-driven transmembrane transporter activity	0.041293091790546	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.0313681511675771	bayes_pls_golite062009
transmembrane transporter activity	0.00294700699983985	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2