Protein Overview: SEC21

General Information:
Name(s) found:	SEC21 / YNL287W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6324042\|ref\|NP_014112.1\| Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo; Sec21p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S63261 SEC21 protein - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1706001\|sp\|P32074.2\|COPG_YEAST RecName: Full=Coatomer subunit gamma; AltName: Full=Gamma-coat protein; Short=Gamma-COP [NCBI NR] gi\|1302369\|emb\|CAA96204.1\| SEC21 [Saccharomyces cerevisiae] [NCBI NR] Coatomer subunit gamma OS=Saccharomyces cerevisiae GN=SEC21 PE=1 SV=2 [Swiss-Prot] SEC21 Coatomer complex gamma chain (gamma-COP) of secretory pathway vesicles [mips] Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo [SGD] Chr XIV from 91992-94799 [yeastorf3.fasta] (P32074) Coatomer gamma subunit (Gamma-coat protein) (Gamma-COP) [4932.FASTACCERV.txt] SEC21, Chr XIV from 91992-94799 [yeast_orfs2] SEC21 SGDID:S0005231, Chr XIV from 91992-94799, Verified ORF [yeastflysequest.fasta] Sec21p [Saccharomyces cerevisiae]gi\|1302369\|emb\|CAA96204.1\| SEC21 [Saccharomyces cerevisiae]gi\|2133126\|pir\|\|S63261 SEC21 protein - yeast (Saccharomyces cerevisiae)gi\|1706001\|sp\|P32074\|COPG_YEAST Coatomer gamma subunit (Gamma-coat protein) (Gamma-COP) [nrdb_11152004_Chlamydomonas] SEC21 SGDID:S000005231, Chr XIV from 91994-94801, Verified ORF, "Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] SEC21 SGDID:S000005231, Chr XIV from 91994-94801, Verified ORF, "Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo" [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo; Sec21p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] SEC21 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Coatomer subunit gamma (Gamma-coat protein) (Gamma-COP) [nr-271106-contam.fasta] [chemostatdb.fasta] Coatomer subunit gamma OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SEC21 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] SEC21 SGDID:S000005231, Chr XIV from 91994-94801, Genome Release 64-1-1, Verified ORF, "Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo" [yeast-030211-contam.fasta] SEC21 SGDID:S000005231, Chr XIV from 91994-94801, Genome Release 64-2-1, Verified ORF, "Gamma subunit of coatomer; coatomer is a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo" [20141208_orf_trans.fasta] TR8803\|c0_g1_i1\|g.6072 ORF TR8803\|c0_g1_i1\|g.6072 TR8803\|c0_g1_i1\|m.6072 type:complete len:936 (+) TR8803\|c0_g1_i1:193-3000(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] TR4513\|c0_g1_i1\|g.3059 ORF TR4513\|c0_g1_i1\|g.3059 TR4513\|c0_g1_i1\|m.3059 type:complete len:936 (+) TR4513\|c0_g1_i1:192-2999(+) [DBVPG6044_S_cerevisiae-Sep2015-contam.fasta] YNL287W SGDID:S000005231, Chr XIV from 91994-94801, Genome Release 64-1-1, Verified ORF, "Gamma subunit of coatomer, a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_5458\|g.3895 ORF asmbl_5458\|g.3895 asmbl_5458\|m.3895 type:complete len:936 (+) asmbl_5458:190-2997(+) [DBVPG6044_Trinity_v2_2_0_PASA_6May2016-contam.fasta] asmbl_9737\|g.7006 ORF asmbl_9737\|g.7006 asmbl_9737\|m.7006 type:complete len:936 (+) asmbl_9737:192-2999(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_5455\|g.3587 ORF ID=asmbl_5455\|g.3587 ID=asmbl_5455\|m.3587 type:complete len:936 (+) ID=asmbl_5455:190-2997(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta] ID=asmbl_9743\|g.5895 ORF ID=asmbl_9743\|g.5895 ID=asmbl_9743\|m.5895 type:complete len:936 (+) ID=asmbl_9743:192-2999(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	935 amino acids
Gene Ontology:
Cellular Component:	endosome [IDA] COPI vesicle coat [IDA]
Biological Process:	ER to Golgi vesicle-mediated transport [IMP] retrograde vesicle-mediated transport, Golgi to ER [IMP]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSAHTYKKFE NSTSGDLPDK MTIYQDCMNT FNESPVNSKR CRLLISRLLR LLAQGETFPQ  60
   61 NEATALFFSI SKLFQHQNDP LRQAVYLAIK ELSGISEDVL MATSSIMKDV QNGSDLIKPD 120
  121 AIRSLTYVLD ESTAFSAERL LKSAVVSRHP SISSAALCTS YHLLPISEVT IRRFTNETQE 180
  181 AVLDLKQFPN QHGNSEYYPN STYISQYHAL GLLYQLKKTD KMALLKLVRH FSENNSMKNQ 240
  241 LAKVELVKIV NDLIYRDPQL FSQFRPLLSD WLSNKFESVQ LETAKLITSF ATRNSRLVAP 300
  301 ELYAAAISAL QSLLTVPRVS SRFAALRILN RISMVSPEKI VVCNPELESL INDSNRNIST 360
  361 YAITTLLKTG TSKNISSLIS TITNFIHDVS DDFKIIIIDA VRTLSLNFPQ EWKSILNFLI 420
  421 DVLKNSEGGF KFKNSIVEAL IDIVSFVPQS KELALENLCD FIEDCEFNEI LVRILHLLGK 480
  481 EGPSAPNPSL YVRHIYNRVV LENSIIRSAA VVALSKFALT KNDPTLYESI ISLLKRIAND 540
  541 KDDEVRDRAT IALEFIDSAR NKDDVIAQNL IESKYFYDIP SLESKLSSYI SSNTDSFATA 600
  601 FDVNQVRKFT EDEMKAINLK RKQEQIFNQK SETTLDTTPE AESVPEKRAD ANSFAGPNLD 660
  661 DHQEDLLATK YADELLSIEQ IKPFGQLVNS SRAISLTEPE AEFVVRGVKH LFKDNVVLQF 720
  721 NITNTLTDIA LDNVSVVCTP EISDEAELEE LFTLQVDRLL PSEEAACYVA FKKLDEIVME 780
  781 GFLNNLTFTT KEINPDTNEP FDGDEGFQDE YEIDSIFLNA GDYVKSSFTG NFSATFDELP 840
  841 CEEVAVFNIQ EDLSIQEVVD KIILNSSCLP VESTQFAPSD SNSHTLKLFG KSALTGSKVA 900
  901 LQIKMIKSSK GLALKVHGKG EDSLLCSDLV NGLMQ

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		COP1 GLO3 RET2 RET3 SEC21 SEC26 SEC27 SEC28
View Details	(MIPS) Mewes HW, et al. (2004)		ARF1 COP1 RET2 RET3 SEC21 SEC26 SEC27 SEC28
View Details	Krogan NJ, et al. (2006)		COP1 FCF2 FMP23 LCB5 PIP2 RET2 RET3 SCS2 SEC2 SEC21 SEC26 SEC27 SEC28
View Details	Gavin AC, et al. (2006)		COP1 RET2 RET3 SEC21 SEC26 SEC27 SEC28
View Details	Gavin AC, et al. (2002)		ADE4 ASC1 BFR2 BMS1 BRX1 BUD21 CBF5 CFT2 CKA1 CKA2 CKB1 CKB2 CMS1 COP1 DBP8 DIA4 DIM1 DIP2 DIS3 ECM16 ECM29 EMG1 ENP1 ENP2 ERO1 ESF1 ESF2 FAP7 FIP1 GCN1 GLC7 GLO3 GPT2 HAS1 HCA4 HHF1, HHF2 HSL1 HTA2 ILV1 IMP3 IMP4 KRE33 KRR1 LCP5 LTV1 MPP10 MRD1 MVD1 NAN1 NOB1 NOC2 NOC4 NOP1 NOP12 NOP14 NOP56 NOP58 NOP6 PAP1 PFS2 PGK1 PNO1 PRE6 PRE9 PRP43 PTA1 PTI1 PWP2 RCL1 REF2 RET2 RET3 RML2 RNA14 ROK1 RPP2B RRP12 RRP7 RRP9 RTG2 SAM1 SCJ1 SCL1 SEC21 SEC26 SEC27 SEC28 SEH1 SLX9 SOF1 SRO9 TIF1, TIF2 TOS1 TSR1 UTP10 UTP11 UTP13 UTP15 UTP18 UTP20 UTP21 UTP22 UTP30 UTP4 UTP6 UTP7 UTP8 UTP9 YOR059C YSH1
View Details	Ho Y, et al. (2002)		ADR1 ARG4 ATP3 AYR1 COP1 COR1 CPR6 ERG27 GPT2 LIA1 MAE1 OM45 PCT1 PDA1 PET10 PET9 PHO86 PIL1 PRB1 PRE10 PRO3 RET2 RNR2 RTN2 RVS161 SEC18 SEC21 SEC26 SEC27 SEC28 TIF34 TIF35 TPA1 TRP3 YNL181W
View Details	Qiu et al. (2008)		APL5 ARF1 ARF2 COG3 COP1 GLO3 RET2 RET3 SAR1 SEC21 SEC22 SEC24 SEC26 SEC27 SEC28 SFB3 SLY1 VRG4 YMD8 YOS1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MIH1	control 1	McCusker D, et al (2007)
View Run	MIH1	control 2	McCusker D, et al (2007)
View Run	MIH1	experimental1 - mih1-1	McCusker D, et al (2007)
View Run	MIH1	experimental2 - mih1-2	McCusker D, et al (2007)
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	BOI1	Sample boi 1 with ha tag from october 2005	McCusker D, et al (2007)
View Run	MLP2	#31 Asynchronous Prep (Protease cleavage)	Keck JM, et al. (2011)
View Run	MLP2	#04 Alpha Factor Prep2	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	SEC21 and ANP1	Huh WK, et al. (2003)
View Data	SEC21 and COP1	Huh WK, et al. (2003)
View Data	SEC21	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..105]	PSI-BLAST	115.70927	Adaptin beta subunit N-terminal fragment
2	View Details	[106..213]	PSI-BLAST	115.70927	Adaptin beta subunit N-terminal fragment
3	View Details	[214..357] [579..650]	PSI-BLAST	115.70927	Adaptin beta subunit N-terminal fragment
4	View Details	[358..451]	PSI-BLAST	115.70927	Adaptin beta subunit N-terminal fragment
5	View Details	[452..578]	PSI-BLAST	115.70927	Adaptin beta subunit N-terminal fragment
6	View Details	[651..749]	MSA	1.106983	View MSA. No confident structure predictions are available.
7	View Details	[750..935]	MSA	3.019968	View MSA. No confident structure predictions are available.

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.94

Protein Overview: SEC21

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: