Protein Overview: MES1

General Information:
Name(s) found:	MES1 / YGR264C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6321704\|ref\|NP_011780.1\| Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs; Mes1p [Saccharomyces cer [NCBI NR] pir\|\|SYBYMT methionine-tRNA ligase (EC 6.1.1.10), cytosolic - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1711639\|sp\|P00958.4\|SYMC_YEAST RecName: Full=Methionyl-tRNA synthetase, cytoplasmic; AltName: Full=Methionine--tRNA ligase; Short=MetRS [NCBI NR] gi\|1536956\|emb\|CAA69086.1\| methionine--tRNA ligase [Saccharomyces cerevisiae] [NCBI NR] gi\|1323481\|emb\|CAA97293.1\| MES1 [Saccharomyces cerevisiae] [NCBI NR] Methionyl-tRNA synthetase, cytoplasmic OS=Saccharomyces cerevisiae GN=MES1 PE=1 SV=4 [Swiss-Prot] MES1 Methionyl-tRNA synthetase [mips] Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs [SGD] MES1, Chr VII from 1019595-1021850, reverse complement [yeast_orfs2] Chr VII from 1019595-1021850, reverse complement [yeastorf3.fasta] Chr VII from 1019602-1021857, reverse complement [yeastorf4.fasta] Chr VII from 1021857-1019602, reverse complement [yeastorf5.fasta] MES1 SGDID:S0003496, Chr VII from 1021858-1019603, reverse complement, Verified ORF [yeastflysequest.fasta] MES1 SGDID:S0003496, Chr VII from 1021859-1019604, reverse complement, Verified ORF [10112005cervSGD.fasta] Mes1p [Saccharomyces cerevisiae]gi\|1323481\|emb\|CAA97293.1\| MES1 [Saccharomyces cerevisiae]gi\|1536956\|emb\|CAA69086.1\| methionine--tRNA ligase [Saccharomyces cerevisiae]gi\|2144553\|pir\|\|SYBYMT methionine-tRNA ligase (EC 6.1.1.10), cytosolic - yeast (Sacc [nrdb_11152004_Chlamydomonas] MES1 SGDID:S000003496, Chr VII from 1021859-1019604, reverse complement, Verified ORF, "Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs; Mes1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Methionyl-tRNA synthetase, cytoplasmic (Methionine--tRNA ligase) (MetRS) [nr-271106-contam.fasta] MES1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] methionine--tRNA ligase [Saccharomyces cerevisiae] [nr-271106-contam.fasta] MES1 SGDID:S000003496, Chr VII from 1021859-1019604, reverse complement, Verified ORF, "Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Methionine--tRNA ligase, cytoplasmic OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MES1 PE=1 SV=4 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] MES1 SGDID:S000003496, Chr VII from 1021853-1019598, Genome Release 64-1-1, reverse complement, Verified ORF, "Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs" [yeast-030211-contam.fasta] MES1 SGDID:S000003496, Chr VII from 1021853-1019598, Genome Release 64-2-1, reverse complement, Verified ORF, "Methionyl-tRNA synthetase; forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs" [20141208_orf_trans.fasta] YGR264C SGDID:S000003496, Chr VII from 1021853-1019598, Genome Release 64-1-1, reverse complement, Verified ORF, "Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	751 amino acids
Gene Ontology:
Cellular Component:	cytoplasm [IDA] methionyl glutamyl tRNA synthetase complex [IDA]
Biological Process:	methionyl-tRNA aminoacylation [IDA]
Molecular Function:	methionine-tRNA ligase activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSFLISFDKS KKHPAHLQLA NNLKIALALE YASKNLKPEV DNDNAAMELR NTKEPFLLFD  60
   61 ANAILRYVMD DFEGQTSDKY QFALASLQNL LYHKELPQQH VEVLTNKAIE NYLVELKEPL 120
  121 TTTDLILFAN VYALNSSLVH SKFPELPSKV HNAVALAKKH VPRDSSSFKN IGAVKIQADL 180
  181 TVKPKDSEIL PKPNERNILI TSALPYVNNV PHLGNIIGSV LSADIFARYC KGRNYNALFI 240
  241 CGTDEYGTAT ETKALEEGVT PRQLCDKYHK IHSDVYKWFQ IGFDYFGRTT TDKQTEIAQH 300
  301 IFTKLNSNGY LEEQSMKQLY CPVHNSYLAD RYVEGECPKC HYDDARGDQC DKCGALLDPF 360
  361 ELINPRCKLD DASPEPKYSD HIFLSLDKLE SQISEWVEKA SEEGNWSKNS KTITQSWLKD 420
  421 GLKPRCITRD LVWGTPVPLE KYKDKVLYVW FDATIGYVSI TSNYTKEWKQ WWNNPEHVSL 480
  481 YQFMGKDNVP FHTVVFPGSQ LGTEENWTML HHLNTTEYLQ YENGKFSKSR GVGVFGNNAQ 540
  541 DSGISPSVWR YYLASVRPES SDSHFSWDDF VARNNSELLA NLGNFVNRLI KFVNAKYNGV 600
  601 VPKFDPKKVS NYDGLVKDIN EILSNYVKEM ELGHERRGLE IAMSLSARGN QFLQENKLDN 660
  661 TLFSQSPEKS DAVVAVGLNI IYAVSSIITP YMPEIGEKIN KMLNAPALKI DDRFHLAILE 720
  721 GHNINKAEYL FQRIDEKKID EWRAKYGGQQ V

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ARC1 GUS1 MES1
View Details	Krogan NJ, et al. (2006)		ARC1 ATG14 CAT8 CCC2 HEK2 IRC20 MCA1 MES1 MGA2 NCS2 NHA1 PET127 PHO89 PHO91 YKL050C YLR363W-A
View Details	Gavin AC, et al. (2006)		ARC1 GUS1 MES1
View Details	Gavin AC, et al. (2002)		ADA2 ADH1 CLU1 FUN19 GCN5 HEM13 HFI1 KAP114 KAP123 MED4 MES1 MNN4 MOT1 MYO1 MYO4 NGG1 NOP1 PFK2 PSK1 PTC1 PUF6 RPB2 RPN9 RPO21 RPT5 RVB2 SGF29 SGF73 SIN3 SIN4 SPT15 SPT20 SPT7 SPT8 TAF1 TAF10 TAF11 TAF12 TAF13 TAF14 TAF2 TAF3 TAF4 TAF5 TAF6 TAF7 TAF8 TAF9 TBF1 TEF4 UBP8 VAS1 VPS1
View Details	Ho Y, et al. (2002)		ADH2 CDC16 CDC33 DIS3 EAF3 ERB1 FIP1 FPR3 HAS1 HHF1, HHF2 HPR1 KAP95 KRE33 KRI1 LOC1 MES1 MFT1 MKT1 MMS4 MUS81 NAM8 NHP2 NOP12 NOP2 NOP6 NUP1 NUP2 NUP60 PAP1 PCT1 PWP1 RAD53 REB1 RNT1 RPC10 RPF2 RRP4 RRP43 RRP6 RTT103 SEC23 SIF2 SIN3 SNU56 SRP1 STO1 TAF14 THO2 TRA1 UBI4 UME1 UTP22 YHB1 YPR090W YRA1
View Details	Qiu et al. (2008)		ARC1 FRS1 FRS2 GUS1 MES1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	MLP2	#25 Asynchronous Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#08 Alpha Factor Prep4-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	HBT1	MES1	1	Unpublished Fields Lab Data
View Screen	UBC7	MES1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	MES1	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..190]	deduced	N/A	No confident structure predictions are available.
2	View Details	[191..211] [234..316] [379..516]	PSI-BLAST	777.0	Methionyl-tRNA synthetase (MetRS); Methionyl-tRNA synthetase (MetRS), Zn-domain
3	View Details	[212..233] [317..378] [517..574] [627..639] [733..751]	PSI-BLAST	777.0	Methionyl-tRNA synthetase (MetRS); Methionyl-tRNA synthetase (MetRS), Zn-domain
4	View Details	[575..626] [640..732]	PSI-BLAST	777.0	Methionyl-tRNA synthetase (MetRS); Methionyl-tRNA synthetase (MetRS), Zn-domain

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
ligase activity, forming aminoacyl-tRNA and related compounds	7.16276509815667	bayes_pls_golite062009
ligase activity, forming carbon-oxygen bonds	7.16276509815667	bayes_pls_golite062009
aminoacyl-tRNA ligase activity	7.13957775963187	bayes_pls_golite062009
ligase activity	6.11211476973434	bayes_pls_golite062009
methionine-tRNA ligase activity	5.54691369460949	bayes_pls_golite062009
isoleucine-tRNA ligase activity	3.76808520367507	bayes_pls_golite062009
glutamate-tRNA ligase activity	2.63681738794687	bayes_pls_golite062009
catalytic activity	2.37674226834122	bayes_pls_golite062009
tyrosine-tRNA ligase activity	1.88984817882585	bayes_pls_golite062009
arginine-tRNA ligase activity	1.50161401062914	bayes_pls_golite062009
tryptophan-tRNA ligase activity	1.14187887833901	bayes_pls_golite062009
nucleotidyltransferase activity	1.06535308346284	bayes_pls_golite062009
cysteine-tRNA ligase activity	0.844943947059557	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.625793556381714	bayes_pls_golite062009
nucleic acid binding	0.449504767632354	bayes_pls_golite062009
glutamine-tRNA ligase activity	0.118292082006187	bayes_pls_golite062009
binding	0.090743795600025	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.74

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2