YRC Public Data Repository - Protein Structure Prediction

Protein:	MES1
Organism:	Saccharomyces cerevisiae
Length:	751 amino acids
Reference:	Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

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Top Sequence Alignment Hits

Listed below are up to the top 10 sequence alignment matches, by species, for the PSI-BLAST search against the protein sequence for MES1.

Description	E-value	Query Range	Subject Range
gi\|190406732 - gi\|190406732\|gb\|EDV09999.1\| methionine-tRNA ligase [Saccharomyces cerevisiae RM11-1a] MES1 - Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which in... SYMC_YEAST - Methionine--tRNA ligase, cytoplasmic OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MES...	0.0	[1..751]	[1..751]
SYMC_SCHPO - Probable methionine--tRNA ligase, cytoplasmic OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843)... SPBC17A3.04c - methionine-tRNA ligase	0.0	[20..748]	[19..778]
MARS - methionyl-tRNA synthetase HGNC:6898\|MIM:15... - synthetase gi\|60656267 - gi\|60656267\|gb\|AAX32697.1\| methionine-tRNA synthetase [synthetic construct]	0.0	[97..750]	[159..821]
SYM_METTH - Methionine--tRNA ligase OS=Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JC... gi\|7437664 - pir\|\|G69177 methionine-tRNA ligase (EC 6.1.1.10) - Methanobacterium thermoautotrophicum (strain Delt...	0.0	[197..746]	[3..540]
SYMC_ARATH - Methionine--tRNA ligase, cytoplasmic OS=Arabidopsis thaliana GN=At4g13780 PE=2 SV=1	0.0	[189..750]	[10..587]
SYM_PYRAB - Methionyl-tRNA synthetase OS=Pyrococcus abyssi GN=metG PE=1 SV=1 SYM_PYRAB - Methionine--tRNA ligase OS=Pyrococcus abyssi (strain GE5 / Orsay) GN=metG PE=1 SV=1	0.0	[196..748]	[2..562]
SYM_GEOSE - Methionine--tRNA ligase OS=Geobacillus stearothermophilus GN=metG PE=3 SV=1	0.0	[194..750]	[2..528]

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Predicted Domain #1

Region A:
Residues: [1-190]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSFLISFDKS KKHPAHLQLA NNLKIALALE YASKNLKPEV DNDNAAMELR NTKEPFLLFD  60
   61 ANAILRYVMD DFEGQTSDKY QFALASLQNL LYHKELPQQH VEVLTNKAIE NYLVELKEPL 120
  121 TTTDLILFAN VYALNSSLVH SKFPELPSKV HNAVALAKKH VPRDSSSFKN IGAVKIQADL 180
  181 TVKPKDSEIL

[Run NCBI BLAST on this sequence.]

Detection Method:

deduced

Shown below is our most confident de novo (Rosetta) prediction for this domain.
Click here to view all matches.

Found no confident structure predictions for this domain.

Predicted Domain #2

Region A:
Residues: [191-211]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 PKPNERNILI TSALPYVNNV P

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [234-316]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 NYNALFICGT DEYGTATETK ALEEGVTPRQ LCDKYHKIHS DVYKWFQIGF DYFGRTTTDK  60
   61 QTEIAQHIFT KLNSNGYLEE QSM

[Run NCBI BLAST on this sequence.]

Region C:
Residues: [379-516]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 SDHIFLSLDK LESQISEWVE KASEEGNWSK NSKTITQSWL KDGLKPRCIT RDLVWGTPVP  60
   61 LEKYKDKVLY VWFDATIGYV SITSNYTKEW KQWWNNPEHV SLYQFMGKDN VPFHTVVFPG 120
  121 SQLGTEENWT MLHHLNTT

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	777.0
Match:	1f4lA_
Description:	Methionyl-tRNA synthetase (MetRS); Methionyl-tRNA synthetase (MetRS), Zn-domain
Matching Structure (courtesy of the PDB):

Predicted functions:

Term	Confidence	Notes
ligase activity, forming aminoacyl-tRNA and related compounds	7.16276509815667	bayes_pls_golite062009
ligase activity, forming carbon-oxygen bonds	7.16276509815667	bayes_pls_golite062009
aminoacyl-tRNA ligase activity	7.13957775963187	bayes_pls_golite062009
ligase activity	6.11211476973434	bayes_pls_golite062009
methionine-tRNA ligase activity	5.54691369460949	bayes_pls_golite062009
isoleucine-tRNA ligase activity	3.76808520367507	bayes_pls_golite062009
glutamate-tRNA ligase activity	2.63681738794687	bayes_pls_golite062009
catalytic activity	2.37674226834122	bayes_pls_golite062009
tyrosine-tRNA ligase activity	1.88984817882585	bayes_pls_golite062009
arginine-tRNA ligase activity	1.50161401062914	bayes_pls_golite062009
tryptophan-tRNA ligase activity	1.14187887833901	bayes_pls_golite062009
nucleotidyltransferase activity	1.06535308346284	bayes_pls_golite062009
cysteine-tRNA ligase activity	0.844943947059557	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	0.625793556381714	bayes_pls_golite062009
nucleic acid binding	0.449504767632354	bayes_pls_golite062009
glutamine-tRNA ligase activity	0.118292082006187	bayes_pls_golite062009
binding	0.090743795600025	bayes_pls_golite062009

Predicted Domain #3

Region A: Residues: [212-233]	1 11 21 31 41 51 \| \| \| \| \| \| 1 HLGNIIGSVL SADIFARYCK GR [Run NCBI BLAST on this sequence.]
Region B: Residues: [317-378]	1 11 21 31 41 51 \| \| \| \| \| \| 1 KQLYCPVHNS YLADRYVEGE CPKCHYDDAR GDQCDKCGAL LDPFELINPR CKLDDASPEP 60 61 KY [Run NCBI BLAST on this sequence.]
Region C: Residues: [517-574]	1 11 21 31 41 51 \| \| \| \| \| \| 1 EYLQYENGKF SKSRGVGVFG NNAQDSGISP SVWRYYLASV RPESSDSHFS WDDFVARN [Run NCBI BLAST on this sequence.]
Region D: Residues: [627-639]	1 11 21 31 41 51 \| \| \| \| \| \| 1 VKEMELGHER RGL [Run NCBI BLAST on this sequence.]
Region E: Residues: [733-751]	1 11 21 31 41 51 \| \| \| \| \| \| 1 RIDEKKIDEW RAKYGGQQV [Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	777.0
Match:	1f4lA_
Description:	Methionyl-tRNA synthetase (MetRS); Methionyl-tRNA synthetase (MetRS), Zn-domain
Matching Structure (courtesy of the PDB):

Predicted Domain #4

Region A:
Residues: [575-626]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 NSELLANLGN FVNRLIKFVN AKYNGVVPKF DPKKVSNYDG LVKDINEILS NY

[Run NCBI BLAST on this sequence.]

Region B:
Residues: [640-732]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 EIAMSLSARG NQFLQENKLD NTLFSQSPEK SDAVVAVGLN IIYAVSSIIT PYMPEIGEKI  60
   61 NKMLNAPALK IDDRFHLAIL EGHNINKAEY LFQ

[Run NCBI BLAST on this sequence.]

Detection Method:	PSI-BLAST
Confidence:	777.0
Match:	1f4lA_
Description:	Methionyl-tRNA synthetase (MetRS); Methionyl-tRNA synthetase (MetRS), Zn-domain
Matching Structure (courtesy of the PDB):

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

View Structure Prediction Details

Top Sequence Alignment Hits

Predicted functions: