Protein Overview: SNF2

General Information:
Name(s) found:	SNF2 / YOR290C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|806532\|dbj\|BAA14423.1\| RIC1 [Saccharomyces cerevisiae] [NCBI NR] gi\|6324864\|ref\|NP_014933.1\| Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p; Snf2p [NCBI NR] gi\|4500\|emb\|CAA40969.1\| GAM1/SNF2 protein [Saccharomyces cerevisiae] [NCBI NR] gi\|172632\|gb\|AAA35059.1\| SNF2protein [Saccharomyces cerevisiae] [NCBI NR] gi\|1420644\|emb\|CAA99517.1\| SNF2 [Saccharomyces cerevisiae] [NCBI NR] gi\|134589\|sp\|P22082.1\|SNF2_YEAST RecName: Full=Transcription regulatory protein SNF2; AltName: Full=ATP-dependent helicase SNF2; AltName: Full=SWI/SNF complex component SNF2; AltName: Full=Regulatory protein SWI2; AltName: Full=Regulatory protein GAM1; Al [NCBI NR] gi\|1279713\|emb\|CAA61793.1\| regulatory protein gam1 [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S15047 SNF2 protein - yeast (Saccharomyces cerevisiae) [NCBI NR] Transcription regulatory protein SNF2 OS=Saccharomyces cerevisiae GN=SNF2 PE=1 SV=1 [Swiss-Prot] SNF2 Component of SWI/SNF global transcription activator complex [mips] Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p [SGD] (P22082) Transcription regulatory protein SNF2 (SWI/SNF complex component SNF2) (Regulatory protein SWI2) (Regulatory protein GAM1) (Transcription factor TYE3) [4932.FASTACCERV.txt] Chr XV from 860255-855144, reverse complement [hmr_yeast5_DM3-1_pombe.fasta] Chr XV from 855143-860254, reverse complement [yeastorf3.fasta] Chr XV from 855144-860255, reverse complement [yeastorf4.fasta] SNF2, Chr XV from 855143-860254, reverse complement [yeast_orfs2] SNF2 SGDID:S0005816, Chr XV from 860255-855144, reverse complement, Verified ORF [yeastflysequest.fasta] Snf2p [Saccharomyces cerevisiae]gi\|1279713\|emb\|CAA61793.1\| regulatory protein gam1 [Saccharomyces cerevisiae]gi\|1420644\|emb\|CAA99517.1\| SNF2 [Saccharomyces cerevisiae]gi\|4500\|emb\|CAA40969.1\| GAM1/SNF2 protein [Saccharomyces cerevisiae]gi\|101629\|pir\|\|S [nrdb_11152004_Chlamydomonas] SNF2 SGDID:S000005816, Chr XV from 860255-855144, reverse complement, Verified ORF, "Catalytic subunit of the 11-subunit SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interd [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] SNF2 SGDID:S000005816, Chr XV from 860257-855146, reverse complement, Verified ORF, "Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently [yeast-contam.fasta] regulatory protein gam1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] GAM1/SNF2 protein [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Transcription regulatory protein SNF2 (ATP-dependent helicase SNF2) (SWI/SNF complex component SNF2) (Regulatory protein SWI2) (Regulatory protein GAM1) (Transcription factor TYE3) [nr-271106-contam.fasta] Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p; Snf2p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] SNF2 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] SNF2protein [nr-271106-contam.fasta] RIC1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] SNF2 SGDID:S000005816, Chr XV from 860257-855146, reverse complement, Verified ORF, "Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Transcription regulatory protein SNF2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SNF2 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] SNF2 SGDID:S000005816, Chr XV from 860258-855147, Genome Release 64-1-1, reverse complement, Verified ORF, "Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p" [yeast-030211-contam.fasta] SNF2 SGDID:S000005816, Chr XV from 860258-855147, Genome Release 64-2-1, reverse complement, Verified ORF, "Catalytic subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p" [20141208_orf_trans.fasta] YOR290C SGDID:S000005816, Chr XV from 860258-855147, Genome Release 64-1-1, reverse complement, Verified ORF, "Catalytic subunit of the SWI/SNF chromatin remodeling complex involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	1703 amino acids
Gene Ontology:
Cellular Component:	SWI/SNF complex [TAS] chromatin remodeling complex [TAS]
Biological Process:	chromatin remodeling [TAS] double-strand break repair [IMP]
Molecular Function:	DNA-dependent ATPase activity [IDA][IMP] general RNA polymerase II transcription factor activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MNIPQRQFSN EEVNRCYLRW QHLRNEHGMN APSVPEFIYL TKVLQFAAKQ RQELQMQRQQ  60
   61 QGISGSQQNI VPNSSDQAEL PNNASSHISA SASPHLAPNM QLNGNETFST SAHQSPIMQT 120
  121 QMPLNSNGGN NMLPQRQSSV GSLNATNFSP TPANNGENAA EKPDNSNHNN LNLNNSELQP 180
  181 QNRSLQEHNI QDSNVMPGSQ INSPMPQQAQ MQQAQFQAQQ AQQAQQAQQA QQAQARLQQG 240
  241 RRLPMTMFTA EQSELLKAQI TSLKCLVNRK PIPFEFQAVI QKSINHPPDF KRMLLSLSEF 300
  301 ARRRQPTDQN NQSNLNGGNN TQQPGTNSHY NNTNTDNVSG LTRNAPLDSK DENFASVSPA 360
  361 GPSSVHNAKN GTLDKNSQTV SGTPITQTES KKEENETISN VAKTAPNSNK THTEQNNPPK 420
  421 PQKPVPLNVL QDQYKEGIKV VDIDDPDMMV DSFTMPNISH SNIDYQTLLA NSDHAKFTIE 480
  481 PGVLPVGIDT HTATDIYQTL IALNLDTTVN DCLDKLLNDE CTESTRENAL YDYYALQLLP 540
  541 LQKAVRGHVL QFEWHQNSLL TNTHPNFLSK IRNINVQDAL LTNQLYKNHE LLKLERKKTE 600
  601 AVARLKSMNK SAINQYNRRQ DKKNKRLKFG HRLIATHTNL ERDEQKRAEK KAKERLQALK 660
  661 ANDEEAYIKL LDQTKDTRIT HLLRQTNAFL DSLTRAVKDQ QKYTKEMIDS HIKEASEEVD 720
  721 DLSMVPKMKD EEYDDDDDNS NVDYYNVAHR IKEDIKKQPS ILVGGTLKDY QIKGLQWMVS 780
  781 LFNNHLNGIL ADEMGLGKTI QTISLLTYLY EMKNIRGPYL VIVPLSTLSN WSSEFAKWAP 840
  841 TLRTISFKGS PNERKAKQAK IRAGEFDVVL TTFEYIIKER ALLSKVKWVH MIIDEGHRMK 900
  901 NAQSKLSLTL NTHYHADYRL ILTGTPLQNN LPELWALLNF VLPKIFNSVK SFDEWFNTPF 960
  961 ANTGGQDKIE LSEEETLLVI RRLHKVLRPF LLRRLKKDVE KELPDKVEKV VKCKMSALQQ1020
 1021 IMYQQMLKYR RLFIGDQNNK KMVGLRGFNN QIMQLKKICN HPFVFEEVED QINPTRETND1080
 1081 DIWRVAGKFE LLDRILPKLK ATGHRVLIFF QMTQIMDIME DFLRYINIKY LRLDGHTKSD1140
 1141 ERSELLRLFN APDSEYLCFI LSTRAGGLGL NLQTADTVII FDTDWNPHQD LQAQDRAHRI1200
 1201 GQKNEVRILR LITTNSVEEV ILERAYKKLD IDGKVIQAGK FDNKSTSEEQ EALLRSLLDA1260
 1261 EEERRKKRES GVEEEEELKD SEINEILARN DEEMAVLTRM DEDRSKKEEE LGVKSRLLEK1320
 1321 SELPDIYSRD IGAELKREES ESAAVYNGRG ARERKTATYN DNMSEEQWLR QFEVSDDEKN1380
 1381 DKQARKQRTK KEDKSEAIDG NGEIKGENID ADNDGPRINN ISAEDRADTD LAMNDDDFLS1440
 1441 KKRKAGRPRG RPKKVKLEGS ENSEPPALES SPVTGDNSPS EDFMDIPKPR TAGKTSVKSA1500
 1501 RTSTRGRGRG RGRGRGRGRG RGRPPKARNG LDYVRTPAAA TSPIDIREKV AKQALDLYHF1560
 1561 ALNYENEAGR KLSDIFLSKP SKALYPDYYM IIKYPVAFDN INTHIETLAY NSLKETLQDF1620
 1621 HLIFSNARIY NTEGSVVYED SLELEKVVTK KYCEIMGDNS QLDFTEFDEQ YGTRPLVLPP1680
 1681 VVTSSVAESF TDEADSSMTE ASV

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ARP7 ARP9 SNF12 SNF2 SNF5 SNF6 SWI1 SWI3 SWP82
View Details	(MIPS) Mewes HW, et al. (2004)		ARP7 ARP9 SNF11 SNF12 SNF2 SNF5 SNF6 SWI1 SWI3 TAF14
View Details	Krogan NJ, et al. (2006)		SNF12 SNF2 SNF5 SNF6 SWI1 SWI3 SWP82
View Details	Gavin AC, et al. (2002)		ADH1 ARP9 ATG26 BEM2 BUD3 CBF5 COP1 CPR1 CYR1 DIM1 ECM16 EMG1 ENP1 HCA4 HHF1, HHF2 HOS2 HOS4 HRR25 IMG1 IMG2 IML1 IMP3 IMP4 KAP95 KRE33 KRR1 LHP1 LTV1 MHR1 MPP10 MRH4 MRM1 MRP20 MRP49 MRP7 MRPL1 MRPL10 MRPL13 MRPL16 MRPL17 MRPL19 MRPL20 MRPL23 MRPL24 MRPL25 MRPL27 MRPL28 MRPL3 MRPL35 MRPL36 MRPL39 MRPL4 MRPL44 MRPL51 MRPL6 MRPL7 MRPL8 NAB3 NAB6 NAM7 NOB1 NOC4 NOP1 NOP14 NPL3 NRD1 NRP1 NUG1 PNO1 POL2 PRP43 PUB1 RCL1 RET2 RIO2 ROM2 RRP12 RTC6 RVB2 SAM1 SEC1 SEC26 SEC27 SET3 SGN1 SIF2 SIN4 SLX9 SMD3 SNF12 SNF2 SNF5 SNF6 SNT1 SOF1 STO1 SWI1 SWI3 SWP82 SYP1 TAF5 TIF4631 TIF4632 TSR1 UTP15 UTP20 UTP22 UTP6 UTP7 VPS1 YDR115W YGR054W YML6
View Details	Qiu et al. (2008)		ARP7 ARP9 SNF11 SNF12 SNF2 SNF5 SNF6 SWI1 SWI3 TAF14 TAF5 TFB5

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CAB3	No Comments	Hazbun TR, et al. (2003)
View Run	LST8	No Comments	Reinke A, et al. (2004)
View Run	TOR2	No Comments	Reinke A, et al. (2004)
View Run	PAN1	No Comments	Toshima J, et al (2006)
View Run	RLF2	phosphorylation data - cascade search	Green EM, et al (2005)
View Run	RLF2	identification data - first search in cascade	Green EM, et al (2005)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	VAM3	Sample: HX11 - both fusion and trans-SNARE complex formation take place.	Hao Xu, et al. (2010)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	RAD51	SNF2	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	SNF2	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..404]	MSA	28.176997	View MSA. No confident structure predictions are available.
2	View Details	[405..708]	deduced	N/A	No confident structure predictions are available.
3	View Details	[709..962]	PSI-BLAST	7.221849	RecG, N-terminal domain; RecG "wedge" domain; RecG helicase domain
4	View Details	[963..1014]	deduced	N/A	No confident structure predictions are available.
5	View Details	[1015..1086]	PSI-BLAST	7.39794	Putative DEAD box RNA helicase
6	View Details	[1087..1347]	PSI-BLAST	5.39794	Nucleotide excision repair enzyme UvrB
7	View Details	[1348..1510]	deduced	N/A	No confident structure predictions are available.
8	View Details	[1511..1703]	PSI-BLAST	87.10721	GCN5

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
binding	2.64537357529343	bayes_pls_golite062009
nucleic acid binding	2.51683451033161	bayes_pls_golite062009
ATP-dependent DNA helicase activity	2.4348680404207	bayes_pls_golite062009
pyrophosphatase activity	2.13284212711072	bayes_pls_golite062009
DNA binding	2.12258000781149	bayes_pls_golite062009
transcription regulator activity	2.09345198747025	bayes_pls_golite062009
helicase activity	1.70242731830224	bayes_pls_golite062009
structure-specific DNA binding	1.2539059700735	bayes_pls_golite062009
protein binding	1.22129154759482	bayes_pls_golite062009
DNA-dependent ATPase activity	1.2155186842031	bayes_pls_golite062009
purine NTP-dependent helicase activity	1.19311444180077	bayes_pls_golite062009
ATP-dependent helicase activity	1.19311444180077	bayes_pls_golite062009
double-stranded DNA binding	1.117585097627	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	1.07968294254548	bayes_pls_golite062009
histone acetyltransferase activity	1.05990253628867	bayes_pls_golite062009
lysine N-acetyltransferase activity	1.05990253628867	bayes_pls_golite062009
catalytic activity	1.00104173493322	bayes_pls_golite062009
transcription activator activity	0.889929694023098	bayes_pls_golite062009
transcription factor activity	0.887108427603557	bayes_pls_golite062009
DNA helicase activity	0.72881469450553	bayes_pls_golite062009
motor activity	0.677658554107	bayes_pls_golite062009
single-stranded DNA binding	0.568457189190858	bayes_pls_golite062009
sequence-specific DNA binding	0.543891899378373	bayes_pls_golite062009
mismatched DNA binding	0.31144582840038	bayes_pls_golite062009
RNA polymerase II transcription factor activity	0.243902356228423	bayes_pls_golite062009
RNA helicase activity	0.18426050934025	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.147579541298736	bayes_pls_golite062009
RNA-dependent ATPase activity	0.14606996093053	bayes_pls_golite062009
ATP-dependent RNA helicase activity	0.13050483816765	bayes_pls_golite062009
microtubule motor activity	0.11674780318211	bayes_pls_golite062009
nucleotide binding	0.114848452385726	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	0.113504049290503	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	0.111293884322157	bayes_pls_golite062009
purine nucleotide binding	0.10713474095071	bayes_pls_golite062009
purine ribonucleotide binding	0.0916519414118148	bayes_pls_golite062009
ribonucleotide binding	0.0915913646217636	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2