Protein Overview: MGM101

General Information:
Name(s) found:	MGM101 / YJR144W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6322604\|ref\|NP_012678.1\| Protein involved in mitochondrial genome maintenance; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage; Mgm101p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S34849 MGM101 protein - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|417271\|sp\|P32787.1\|MG101_YEAST RecName: Full=Mitochondrial genome maintenance protein MGM101; Flags: Precursor [NCBI NR] gi\|3955\|emb\|CAA48502.1\| MGM101 [Saccharomyces cerevisiae] [NCBI NR] gi\|1015890\|emb\|CAA89677.1\| MGM101 [Saccharomyces cerevisiae] [NCBI NR] Mitochondrial genome maintenance protein MGM101 OS=Saccharomyces cerevisiae GN=MGM101 PE=1 SV=1 [Swiss-Prot] (P32787) Mitochondrial genome maintenance protein MGM101, mitochondrial precursor [Swiss-Prot] MGM101 Mitochondrial genome maintenance protein [mips] Protein involved in mitochondrial genome maintenance; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage [SGD] MGM101, Chr X from 700575-701384 [yeast_orfs2] Chr X from 700575-701384 [yeastorf3.fasta] Chr X from 700576-701385 [yeastorf4.fasta] Chr X from 700577-701386 [yeastorf5.fasta] MGM101 SGDID:S0003905, Chr X from 700797-701606, Verified ORF [yeastflysequest.fasta] Involved in mitochondrial genome maintenance; (putative) nucleic acid interactor [Saccharomyces cerevisiae]gi\|1015890\|emb\|CAA89677.1\| MGM101 [Saccharomyces cerevisiae]gi\|3955\|emb\|CAA48502.1\| MGM101 [Saccharomyces cerevisiae]gi\|479602\|pir\|\|S34849 MGM101 [nrdb_11152004_Chlamydomonas] MGM101 SGDID:S000003905, Chr X from 700797-701606, Verified ORF, "Protein involved in mitochondrial genome maintenance; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] MGM101 SGDID:S000003905, Chr X from 700876-701685, Verified ORF, "Protein involved in mitochondrial genome maintenance; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage" [yeast-contam.fasta] MGM101 SGDID:S000003905, Chr X from 700873-701682, Verified ORF, "Protein involved in mitochondrial genome maintenance; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage" [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] MGM101 SGDID:S000003905, Chr X from 700872-701681, Verified ORF, "Protein involved in mitochondrial genome maintenance; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage" [yeast-200209-contam.fasta] MGM101 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Mitochondrial genome maintenance protein MGM101, mitochondrial precursor [nr-271106-contam.fasta] Protein involved in mitochondrial genome maintenance; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage; Mgm101p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] Mitochondrial genome maintenance protein MGM101 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MGM101 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] MGM101 SGDID:S000003905, Chr X from 700882-701691, Genome Release 64-1-1, Verified ORF, "Protein involved in mitochondrial genome maintenance; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage" [yeast-030211-contam.fasta] MGM101 SGDID:S000003905, Chr X from 700882-701691, Genome Release 64-2-1, Verified ORF, "Protein with a role in mitochondrial DNA recombinational repair; also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage and mitochondrial genome maintenance" [20141208_orf_trans.fasta] YJR144W SGDID:S000003905, Chr X from 700882-701691, Genome Release 64-1-1, Verified ORF, "Protein involved in mitochondrial genome maintenance; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	269 amino acids
Gene Ontology:
Cellular Component:	mitochondrial chromosome [IDA] mitochondrion [IDA] mitochondrial nucleoid [IDA]
Biological Process:	DNA repair [IMP] mitochondrial genome maintenance [IMP]
Molecular Function:	DNA binding [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MKSIFKVRGC VSHAAQFCQK RTVVSTGTSN TATAGAVRKS FNSTETKPVF ATKSEAGNGS  60
   61 HMKEYSSGIN SKLGGTPLET RSTADDSLNN SYKQVKGDID WYTSWYGLGM KPFEAKVQKD 120
  121 LIEPLDPKDI EIKPDGLIYL PEIKYRRILN KAFGAGGWGL VPRSQTIVTS KLVTREYGLI 180
  181 CHGQLISVAR GEQDYFNEAG IPTATEGCKS NALMRCCKDL GVGSELWDPV FIKKFKVDHC 240
  241 TEKFVEHVTT KRKKKIWLRK DRQVEYPYK

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		MGM101 MPH1
View Details	Gavin AC, et al. (2002)		CMR1 DNA2 HDA1 HDA2 HDA3 HSC82 KAP95 LCD1 MEC1 MGM101 MLH1 MPH1 MSH2 MSH3 MSH6 OLA1 PGK1 PMS1 PRO2 RAD16 RAD52 RFA1 RFA2 RFA3 RIM1 RNH1 RSC8 RVB2 SGS1 SHS1 TIF1, TIF2 TOP3 VAS1 VPS1 YHR122W
View Details	Ho Y, et al. (2002)		ADH2 AIM21 AIM46 ARE1 BUD20 CBF5 CBP2 CCM1 CDC10 CDC28 CDC33 CDC5 CEG1 CIC1 CKA1 CKA2 CKB1 CKB2 COF1 CPR1 DBP2 DBP7 EGD1 EHD3 ENP2 ERB1 FET4 FKH1 FPR3 FUN12 FYV8 GBP2 GCD2 GCD7 GCN3 GPI15 GRS1 HAS1 HHF1, HHF2 HHO1 HHT1, HHT2 HIR3 HMO1 HOT1 HTA1 HTB1 HTB2 HYP2 ICP55 IMD1 IMD2 IMD3 IMD4 ISA1 KRE33 KRI1 LOC1 LSM2 MAG2 MAM33 MBP1 MDH1 MGM101 MPH1 MRPS16 MSC3 MSS116 MUS81 NET1 NOC2 NOG1 NOP1 NOP12 NOP2 NOP4 NOP56 NOP6 NPL3 NUG1 OYE2 PDC6 PDI1 PET127 POB3 POL5 PRP43 PSO2 PUF6 PWP1 RFA2 RLI1 RLP24 RPF2 RRP1 RRP12 RRP5 SEC2 SIN3 SPT16 SSF1 SUI2 SUI3 TAF14 TEF4 TIF4631 TIF4632 TIF6 TMA29 TRA1 TSR1 UBP12 URB1 URB2 URE2 UTP10 UTP20 UTP22 YER084W YFL006W YGR017W YMR144W YPP1 YRA1 YTM1
View Details	Qiu et al. (2008)		ABF2 MGM101 YHM2

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RLF2	phosphorylation data - cascade search	Green EM, et al (2005)
View Run	BOI2	Boi 2 gst control	McCusker D, et al (2007)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	SPC97	MGM101	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..77]	deduced	N/A	Confident ab initio structure predictions are available.
2	View Details	[78..269]	MSA	1.003908	View MSA. No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
binding	2.58949805002675	bayes_pls_golite062009
nucleic acid binding	1.86070042016282	bayes_pls_golite062009
protein binding	1.54537643602694	bayes_pls_golite062009
structure-specific DNA binding	1.24523960036293	bayes_pls_golite062009
DNA-dependent ATPase activity	0.872400487455321	bayes_pls_golite062009
RNA binding	0.686982923008616	bayes_pls_golite062009
structural constituent of ribosome	0.635289190073156	bayes_pls_golite062009
structural molecule activity	0.552042000067166	bayes_pls_golite062009
DNA binding	0.458518652485702	bayes_pls_golite062009
DNA helicase activity	0.404666699673269	bayes_pls_golite062009
cytoskeletal protein binding	0.395139311341453	bayes_pls_golite062009
ATP-dependent DNA helicase activity	0.143807478857287	bayes_pls_golite062009
catalytic activity	0.00430305721638241	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.90

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2