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Protein Overview: SSF1

General Information:
Name(s) found: SSF1 / YHR066W [SGD]
Description(s) found:
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  • gi|731668|sp|P38789.1|SSF1_YEAST RecName: Full=Ribosome biogenesis protein SSF1 [NCBI NR]
  • gi|6321857|ref|NP_011933.1| Constituent of 66S pre-ribosomal particles, required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family; Ssf1p [Saccharomyces cerevisiae] [NCBI NR]
  • pir||S46700 SSF1 protein - yeast (Saccharomyces cerevisiae) [NCBI NR]
  • gi|603207|gb|AAA79980.1| Ssf1p [NCBI NR]
  • gi|487948|gb|AAB68379.1| Ssf1p [Saccharomyces cerevisiae] [NCBI NR]
  • Ribosome biogenesis protein SSF1 OS=Saccharomyces cerevisiae GN=SSF1 PE=1 SV=1 [Swiss-Prot]
  • SSF1 Nucleolar protein involved in the assembly of the large ribosomal subunit [mips]
  • Constituent of 66S pre-ribosomal particles, required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family [SGD]
  • SSF1, Chr VIII from 229336-230697 [yeast_orfs2]
  • Chr VIII from 229336-230697 [yeastorf3.fasta]
  • (P38789) Ribosome biogenesis protein SSF1 [4932.FASTACCERV.txt]
  • SSF1 SGDID:S0001108, Chr VIII from 229336-230697, Verified ORF [yeastflysequest.fasta]
  • Ssf1p [Saccharomyces cerevisiae]gi|731668|sp|P38789|SSF1_YEAST Ribosome biogenesis protein SSF1gi|626624|pir||S46700 SSF1 protein - yeast (Saccharomyces cerevisiae)gi|487948|gb|AAB68379.1| Ssf1p [Saccharomyces cerevisiae]gi|603207|gb|AAA79980.1| Ssf1p [nrdb_11152004_Chlamydomonas]
  • SSF1 SGDID:S000001108, Chr VIII from 229337-230698, Verified ORF, "Constituent of 66S pre-ribosomal particles, required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta]
  • Ssf1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • Constituent of 66S pre-ribosomal particles, required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family; Ssf1p [Saccharomyces cerevisiae] [nr-271106-contam.fasta]
  • Ssf1p [nr-271106-contam.fasta]
  • Ribosome biogenesis protein SSF1 [nr-271106-contam.fasta]
  • [chemostatdb.fasta]
  • Ribosome biogenesis protein SSF1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SSF1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta]
  • SSF1 SGDID:S000001108, Chr VIII from 229335-230696, Genome Release 64-1-1, Verified ORF, "Constituent of 66S pre-ribosomal particles, required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family" [yeast-030211-contam.fasta]
  • SSF1 SGDID:S000001108, Chr VIII from 229335-230696, Genome Release 64-2-1, Verified ORF, "Constituent of 66S pre-ribosomal particles; required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family; SSF1 has a paralog, SSF2, that arose from the whole genome duplication" [20141208_orf_trans.fasta]
  • YHR066W SGDID:S000001108, Chr VIII from 229335-230696, Genome Release 64-1-1, Verified ORF, "Constituent of 66S pre-ribosomal particles, required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
    • Organism: Saccharomyces cerevisiae
    Length: 453 amino acids

    Gene Ontology:
    Cellular Component: nucleolus [IDALink to PubMed article]
    Biological Process: conjugation with cellular fusion [IGILink to PubMed article]
    regulation of cell size [IMPLink to PubMed article]
    ribosomal large subunit assembly [IMPLink to PubMed article][IPILink to PubMed article]
    Molecular Function: rRNA binding [IPILink to PubMed article]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MAKRRQKKRT HAQLTPEQEQ GIPKSMVIRV GQTSLANHSL NQLVKDFRQI MQPHTAIKLK  60
   61 ERKSNKLKDF VVMCGPLGVT HLFMFTQSEK TGNVSLKIAR TPQGPTVTFQ VLDYSLGRDI 120
  121 KKFLKRPKSL NNDDVLNPPL LVLNGFSTSK RSGEDDQDVN VEKVIVSMFQ NIFPPLNPAR 180
  181 TSLNSIKRVF MINKDRETGE ISMRHYFIDI REVEISRNLK RLYKAKNNLS KTVPNLHRKE 240
  241 DISSLILDHD LGAYTSESEI EDDAIVRVVD NQDVKAKHSQ SLKSQRTPVE KKDNKEREKE 300
  301 TEEEDVEMEE PKPSENLQPT PRKKAIKLTE LGPRLTLKLV KIEEGICSGK VLHHEFVQKS 360
  361 SEEIKALEKR HAAKMRLKEQ RKKEQEENIA KKKAVKDAKK QRKLERRKAR AAEGGEGQGK 420
  421 DDAMSDDESS SSDSEHYGSV PEDLDSDLFS EVE

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) DBP10 PUF6 SSF1 TIF6 URB1
    View Details Krogan NJ, et al. (2006) RRP14 RRP15 SSF1 SSF2 URB1
    View Details Gavin AC, et al. (2002) ARX1 BEM2 BMS1 BRX1 BUD20 CIC1 CLU1 CYR1 CYS4 DBP10 DBP9 DCP2 DRS1 EBP2 ERB1 FPR4 GCN1 HAS1 HDA1 HER1 HIS3 INP2 IPI1 IPI3 KRE33 LHP1 LOC1 LSG1 MAK21 MAK5 MDN1 MIS1 MRT4 NAP1 NIP1 NIP7 NMD3 NOC2 NOC3 NOG1 NOG2 NOP1 NOP12 NOP15 NOP16 NOP2 NOP4 NOP56 NOP7 NSA1 NSA2 NUG1 PRT1 PUF6 REI1 RIX1 RIX7 RLP24 RLP7 RPA135 RPA190 RPF1 RPF2 RPG1 RPP2B RPT6 RRP1 RRP12 RSA3 RSA4 RTR1 SDA1 SEC7 SKI2 SNU13 SPB1 SPB4 SSF1 TIF6 TUB3 URB1 UTP10 UTP20 UTP22 YGL036W YTM1
    View Details Ho Y, et al. (2002) AAC3 BMS1 CDC53 CIC1 CKA1 CKA2 CKB1 CKB2 COF1 CTF4 CYS4 DBP10 DBP2 DED81 DIA2 EGD1 ENP1 ENP2 ERB1 FPR3 GDE1 GND1 GSP1 HAS1 HHF1, HHF2 HHT1, HHT2 HOT1 HTA1 HTB2 ILS1 KRE33 KRI1 LST4 MCM2 MCM3 MCM5 MGM101 MRPL1 NIP7 NMD3 NOC3 NOG1 NOP12 NOP2 NOP4 NOP56 NOP7 NUG1 PDI1 POB3 POL2 PUF6 PWP1 RLI1 RPF2 RRP12 RRP14 RRP5 SEH1 SFP1 SKP1 SLT2 SPB1 SPT16 SPT2 SRP1 SSF1 SSF2 SSZ1 STE23 SUP45 TIF4631 TIF6 TRL1 URB1 UTP10 UTP22 UTP7 VMR1 VPS73 WTM2 YAK1 YBL104C YER084W YRA1
    View Details Qiu et al. (2008) BRX1 DRS1 GAR1 NOP15 NSR1 PXR1 RPF2 RPL27A SSF1 SSF2 UTP13 UTP9

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run MLP2 #24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    SHOWING SINGLE HITS. [ Hide Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

    No yeast two-hybrid interactions found for this protein.

    Microscopy / Localization Data

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      PROTEIN(S) PUBLICATION
    View Data SSF1 and NOP56 Huh WK, et al. (2003)
    View Data SSF1 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..294] Pfam 172.79588 No description for PF03877 was found. No confident structure predictions are available.
    2 View Details [295..372] deduced N/A Confident ab initio structure predictions are available.
    3 View Details [373..453] MSA 2.01199 View MSA. No confident structure predictions are available.

    Functions predicted (by domain):

    # Gene Ontology predictions
    1
    Term Confidence Notes
  • binding
    		•  1.74559657602427 bayes_pls_golite062009
  • protein binding
    		•  0.360840654168616 bayes_pls_golite062009
    2 No functions predicted.
    3 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.70

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle