Protein Overview: MCM2

General Information:
Name(s) found:	MCM2 / YBL023C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|6319448\|ref\|NP_009530.1\| Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; Mcm2p [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S45757 replication licensing factor MCM2 [validated] - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|602896\|emb\|CAA54503.1\| MCM2 [Saccharomyces cerevisiae] [NCBI NR] gi\|585465\|sp\|P29469.2\|MCM2_YEAST RecName: Full=DNA replication licensing factor MCM2; AltName: Full=Minichromosome maintenance protein 2 [NCBI NR] gi\|536021\|emb\|CAA84842.1\| MCM2 [Saccharomyces cerevisiae] [NCBI NR] DNA replication licensing factor MCM2 OS=Saccharomyces cerevisiae GN=MCM2 PE=1 SV=2 [Swiss-Prot] MCM2 Member of the Mcm2p,Mcm3p,Cdc46p family [mips] Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex [SGD] MCM2, Chr II from 174882-177488, reverse complement [yeast_orfs2] Chr II from 174882-177488, reverse complement [yeastorf3.fasta] Chr II from 177488-174882, reverse complement [yeastorf5.fasta] (P29469) DNA replication licensing factor MCM2 (Minichromosome maintenance protein 2) [4932.FASTACCERV.txt] MCM2 SGDID:S0000119, Chr II from 177491-174885, reverse complement, Verified ORF [yeastflysequest.fasta] MCM2 SGDID:S0000119, Chr II from 177529-174923, reverse complement, Verified ORF [10112005cervSGD.fasta] Mcm2p [Saccharomyces cerevisiae]gi\|602896\|emb\|CAA54503.1\| MCM2 [Saccharomyces cerevisiae]gi\|536021\|emb\|CAA84842.1\| MCM2 [Saccharomyces cerevisiae]gi\|585465\|sp\|P29469\|MCM2_YEAST DNA replication licensing factor MCM2 (Minichromosome maintenance protein 2 [nrdb_11152004_Chlamydomonas] MCM2 SGDID:S000000119, Chr II from 177529-174923, reverse complement, Verified ORF, "Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex" [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; Mcm2p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] DNA replication licensing factor MCM2 (Minichromosome maintenance protein 2) [nr-271106-contam.fasta] MCM2 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] [chemostatdb.fasta] DNA replication licensing factor MCM2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MCM2 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] MCM2 SGDID:S000000119, Chr II from 177526-174920, Genome Release 64-1-1, reverse complement, Verified ORF, "Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex" [yeast-030211-contam.fasta] MCM2 SGDID:S000000119, Chr II from 177526-174920, Genome Release 64-2-1, reverse complement, Verified ORF, "Protein involved in DNA replication; component of the Mcm2-7 hexameric helicase complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress" [20141208_orf_trans.fasta] TR2058\|c0_g1_i1\|g.1544 ORF TR2058\|c0_g1_i1\|g.1544 TR2058\|c0_g1_i1\|m.1544 type:complete len:869 (+) TR2058\|c0_g1_i1:131-2737(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] YBL023C SGDID:S000000119, Chr II from 177526-174920, Genome Release 64-1-1, reverse complement, Verified ORF, "Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] ID=asmbl_4927\|g.2834 ORF ID=asmbl_4927\|g.2834 ID=asmbl_4927\|m.2834 type:complete len:869 (+) ID=asmbl_4927:131-2737(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	868 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA] pre-replicative complex [IDA] cytoplasm [IDA][TAS] MCM complex [IDA]
Biological Process:	DNA replication initiation [TAS] pre-replicative complex assembly [IPI] DNA unwinding involved in replication [TAS]
Molecular Function:	chromatin binding [TAS] ATP-dependent DNA helicase activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSDNRRRRRE EDDSDSENEL PPSSPQQHFR GGMNPVSSPI GSPDMINPEG DDNEVDDVPD  60
   61 IDEVEEQMNE VDLMDDNMYE DYAADHNRDR YDPDQVDDRE QQELSLSERR RIDAQLNERD 120
  121 RLLRNVAYID DEDEEQEGAA QLDEMGLPVQ RRRRRRQYED LENSDDDLLS DMDIDPLREE 180
  181 LTLESLSNVK ANSYSEWITQ PNVSRTIARE LKSFLLEYTD ETGRSVYGAR IRTLGEMNSE 240
  241 SLEVNYRHLA ESKAILALFL AKCPEEMLKI FDLVAMEATE LHYPDYARIH SEIHVRISDF 300
  301 PTIYSLRELR ESNLSSLVRV TGVVTRRTGV FPQLKYVKFN CLKCGSILGP FFQDSNEEIR 360
  361 ISFCTNCKSK GPFRVNGEKT VYRNYQRVTL QEAPGTVPPG RLPRHREVIL LADLVDVSKP 420
  421 GEEVEVTGIY KNNYDGNLNA KNGFPVFATI IEANSIKRRE GNTANEGEEG LDVFSWTEEE 480
  481 EREFRKISRD RGIIDKIISS MAPSIYGHRD IKTAVACSLF GGVPKNVNGK HSIRGDINVL 540
  541 LLGDPGTAKS QILKYVEKTA HRAVFATGQG ASAVGLTASV RKDPITKEWT LEGGALVLAD 600
  601 KGVCLIDEFD KMNDQDRTSI HEAMEQQSIS ISKAGIVTTL QARCSIIAAA NPNGGRYNST 660
  661 LPLAQNVSLT EPILSRFDIL CVVRDLVDEE ADERLATFVV DSHVRSHPEN DEDREGEELK 720
  721 NNGESAIEQG EDEINEQLNA RQRRLQRQRK KEEEISPIPQ ELLMKYIHYA RTKIYPKLHQ 780
  781 MDMDKVSRVY ADLRRESIST GSFPITVRHL ESILRIAESF AKMRLSEFVS SYDLDRAIKV 840
  841 VVDSFVDAQK VSVRRQLRRS FAIYTLGH

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	(MIPS) Mewes HW, et al. (2004)		CDC45 CDC6 DPB11 DPB2 DPB3 MCM2 MCM3 MCM4 MCM5 MCM6 MCM7 ORC1 ORC2 ORC3 ORC4 ORC5 ORC6 POL2 POL3 POL31 POL32 SLD3
View Details	Krogan NJ, et al. (2006)		DPB2 DPB3 DPB4 INM1 MBA1 MCM2 POL2 RPN4
View Details	Gavin AC, et al. (2006)		GLN1 MCM2 MKS1 TSC10
View Details	Gavin AC, et al. (2002)		CDC45 CTR3 DAL3 ERG26 GLN1 GLT1 GTO3 HAT1 HSP42 IML1 LYS12 MCM2 MCM5 MCM6 MCM7 MKS1 ORC1 ORC2 ORC3 ORC4 ORC5 ORC6 REG1 RPD3 RPN9 SIN3 TFP1 TIF1, TIF2 TPS1 TPS2 TPS3 TSC10 TSL1 YIL177C
View Details	Ho Y, et al. (2002)		BMS1 CDC53 CIC1 COF1 CTF4 DBP10 DED81 DIA2 ENP1 FPR3 ILS1 KRE33 KRI1 LST4 MCM2 MCM3 MCM5 NIP7 NMD3 NOP12 RRP12 SEH1 SKP1 SLT2 SPB1 SSF1 SSF2 SSZ1 TIF6 URB1 UTP10 YAK1 YBL104C
View Details	Qiu et al. (2008)		CDC45 CDC6 DPB11 DPB2 DPB3 DPB4 MCM2 MCM3 MCM4 MCM5 MCM6 MCM7 ORC1 ORC2 ORC3 ORC4 ORC5 ORC6 POB3 POL2 POL3 POL30 POL31 POL32 RFC1 RRM3 SLD3

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	MLP2	#29 Asynchronous Prep5-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..121]	MSA	1.015984	View MSA. No confident structure predictions are available.
2	View Details	[122..175]	deduced	N/A	No confident structure predictions are available.
3	View Details	[176..297]	MSA	1.095989	View MSA. Confident ab initio structure predictions are available.
4	View Details	[298..477]	Pfam	7.221849	MCM2/3/5 family No confident structure predictions are available.
5	View Details	[478..496] [688..800]	PSI-BLAST	2.522879	Replication factor C
6	View Details	[497..687]	PSI-BLAST	2.522879	Replication factor C
7	View Details	[801..868]	MSA	1.494987	View MSA. No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
nucleic acid binding	3.99127970564994	bayes_pls_golite062009
DNA binding	3.53568546959418	bayes_pls_golite062009
structural constituent of ribosome	3.51998515668903	bayes_pls_golite062009
structural molecule activity	3.29857127235306	bayes_pls_golite062009
structure-specific DNA binding	3.06066809614422	bayes_pls_golite062009
single-stranded DNA binding	3.0183551365402	bayes_pls_golite062009
binding	2.83465364476231	bayes_pls_golite062009
telomerase inhibitor activity	2.77979101622935	bayes_pls_golite062009
DNA replication origin binding	1.84819558112187	bayes_pls_golite062009
transcription regulator activity	1.84350127337404	bayes_pls_golite062009
telomeric DNA binding	1.7847835613194	bayes_pls_golite062009
single-stranded telomeric DNA binding	1.48033977789232	bayes_pls_golite062009
translation regulator activity	1.46320772716954	bayes_pls_golite062009
translation factor activity, nucleic acid binding	1.40718318783582	bayes_pls_golite062009
translation initiation factor activity	1.27539679024898	bayes_pls_golite062009
ribonuclease activity	1.19236946227657	bayes_pls_golite062009
DNA helicase activity	1.12203697857261	bayes_pls_golite062009
transcription factor activity	0.952569279670437	bayes_pls_golite062009
sequence-specific DNA binding	0.85691075793641	bayes_pls_golite062009
DNA-directed RNA polymerase activity	0.79554655100991	bayes_pls_golite062009
RNA polymerase activity	0.79554655100991	bayes_pls_golite062009
DNA-dependent ATPase activity	0.638831894247026	bayes_pls_golite062009
nuclease activity	0.57381531077345	bayes_pls_golite062009
aminoacyl-tRNA ligase activity	0.474251377131502	bayes_pls_golite062009
ligase activity, forming carbon-oxygen bonds	0.47336621289748	bayes_pls_golite062009
ligase activity, forming aminoacyl-tRNA and related compounds	0.47336621289748	bayes_pls_golite062009
nucleotidyltransferase activity	0.325774164899685	bayes_pls_golite062009
exonuclease activity	0.28313884527829	bayes_pls_golite062009
RNA binding	0.18502033889261	bayes_pls_golite062009
protein binding	0.152645195799531	bayes_pls_golite062009
exoribonuclease activity, producing 5'-phosphomonoesters	0.125256208482441	bayes_pls_golite062009
exoribonuclease activity	0.109774918833391	bayes_pls_golite062009
exonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 5'-phosphomonoesters	0.0954216620147701	bayes_pls_golite062009
ATP-dependent DNA helicase activity	0.0179535526984695	bayes_pls_golite062009
helicase activity	0.0121541775159524	bayes_pls_golite062009

Term	Confidence	Notes
DNA clamp loader activity	4.91429252297969	bayes_pls_golite062009
protein-DNA loading ATPase activity	4.80695929076807	bayes_pls_golite062009
DNA-dependent ATPase activity	2.72462789867829	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	2.66107861968804	bayes_pls_golite062009
binding	2.64537357529343	bayes_pls_golite062009
DNA helicase activity	2.2817164193758	bayes_pls_golite062009
nucleic acid binding	2.21184938313192	bayes_pls_golite062009
pyrophosphatase activity	2.13284212711072	bayes_pls_golite062009
DNA binding	1.94055378599526	bayes_pls_golite062009
ATP-dependent DNA helicase activity	1.88455339138556	bayes_pls_golite062009
transcription regulator activity	1.83584389083042	bayes_pls_golite062009
helicase activity	1.70242731830224	bayes_pls_golite062009
DNA replication origin binding	1.62491387774159	bayes_pls_golite062009
structural constituent of ribosome	1.47858140861683	bayes_pls_golite062009
microtubule motor activity	1.35938517416356	bayes_pls_golite062009
purine NTP-dependent helicase activity	1.19311444180077	bayes_pls_golite062009
ATP-dependent helicase activity	1.19311444180077	bayes_pls_golite062009
catalytic activity	1.00104173493322	bayes_pls_golite062009
DNA-directed DNA polymerase activity	0.971476020912368	bayes_pls_golite062009
DNA polymerase activity	0.928091843881235	bayes_pls_golite062009
purine ribonucleotide binding	0.923452757551011	bayes_pls_golite062009
ribonucleotide binding	0.923391844349459	bayes_pls_golite062009
purine nucleotide binding	0.920821955769652	bayes_pls_golite062009
nucleotide binding	0.917754212279227	bayes_pls_golite062009
structural molecule activity	0.86514677273731	bayes_pls_golite062009
structure-specific DNA binding	0.782430219754236	bayes_pls_golite062009
transcription factor activity	0.697256579145077	bayes_pls_golite062009
motor activity	0.61636596887462	bayes_pls_golite062009
double-stranded DNA binding	0.6071273686445	bayes_pls_golite062009
ATP binding	0.60527666756293	bayes_pls_golite062009
adenyl ribonucleotide binding	0.573214556107072	bayes_pls_golite062009
adenyl nucleotide binding	0.554530910579109	bayes_pls_golite062009
translation regulator activity	0.29096092258568	bayes_pls_golite062009
translation factor activity, nucleic acid binding	0.274994961137851	bayes_pls_golite062009
RNA helicase activity	0.18426050934025	bayes_pls_golite062009
protein binding	0.170837278876362	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.147579541298736	bayes_pls_golite062009
RNA-dependent ATPase activity	0.14606996093053	bayes_pls_golite062009
ATP-dependent RNA helicase activity	0.13050483816765	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides	0.113504049290503	bayes_pls_golite062009
hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides	0.111293884322157	bayes_pls_golite062009
3'-5' DNA helicase activity	0.0769112340058804	bayes_pls_golite062009
cytoskeletal protein binding	0.0272673919333979	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

	PROTEIN(S)	PUBLICATION
View Data	MCM2	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Protein Overview: MCM2

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: