Protein Overview: ENO2

General Information:
Name(s) found:	ENO2 / YHR174W [SGD]
Description(s) found: SHOW ONLY BEST	pir\|\|NOBY2 phosphopyruvate hydratase (EC 4.2.1.11) 2 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|6321968\|ref\|NP_012044.1\| Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is induced in response to glucose; [NCBI NR] gi\|458897\|gb\|AAB68019.1\| Eno2p: Enolase 2; 2-phosphoglycerate dehydratase [Saccharomyces cerevisiae] [NCBI NR] gi\|171457\|gb\|AAA88713.1\| enolase [NCBI NR] gi\|119337\|sp\|P00925.2\|ENO2_YEAST RecName: Full=Enolase 2; AltName: Full=2-phosphoglycerate dehydratase 2; AltName: Full=2-phospho-D-glycerate hydro-lyase 2 [NCBI NR] Enolase 2 OS=Saccharomyces cerevisiae GN=ENO2 PE=1 SV=2 [Swiss-Prot] ENO2 Enolase II (2-phosphoglycerate dehydratase) [mips] Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is induced in response to glucose [SGD] ENO2, Chr VIII from 451325-452638 [yeast_orfs2] Chr VIII from 451325-452638 [yeastorf3.fasta] ENO2 SGDID:S0001217, Chr VIII from 451325-452638, Verified ORF [yeastflysequest.fasta] ENO2 SGDID:S0001217, Chr VIII from 451326-452639, Verified ORF [10112005cervSGD.fasta] Eno2p [Saccharomyces cerevisiae]gi\|119337\|sp\|P00925\|ENO2_YEAST Enolase 2 (2-phosphoglycerate dehydratase) (2-phospho-D-glycerate hydro-lyase)gi\|68309\|pir\|\|NOBY2 phosphopyruvate hydratase (EC 4.2.1.11) 2 - yeast (Saccharomyces cerevisiae)gi\|458897\|gb\|A [nrdb_11152004_Chlamydomonas] ENO2 SGDID:S000001217, Chr VIII from 451327-452640, Verified ORF, "Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expres [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is induced in response to glucose; Eno2p [Saccharomyces cerevi [nr-271106-contam.fasta] Eno2p: Enolase 2; 2-phosphoglycerate dehydratase [Saccharomyces cerevisiae] [nr-271106-contam.fasta] enolase [nr-271106-contam.fasta] Enolase 2 (2-phosphoglycerate dehydratase 2) (2-phospho-D-glycerate hydro-lyase 2) [nr-271106-contam.fasta] ENO2 SGDID:S000001217, Chr VIII from 451327-452640, Verified ORF, "Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is induced in response to glucose" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Enolase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ENO2 PE=1 SV=2 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] ENO2 SGDID:S000001217, Chr VIII from 451327-452640, Genome Release 64-1-1, Verified ORF, "Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is induced in response to glucose" [yeast-030211-contam.fasta] ENO2 SGDID:S000001217, Chr VIII from 451327-452640, Genome Release 64-2-1, Verified ORF, "Enolase II, a phosphopyruvate hydratase; catalyzes conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression induced in response to glucose; ENO2 has a paralog, ENO1, that arose from the whole genome duplication" [20141208_orf_trans.fasta] TR930\|c0_g1_i1\|g.569 ORF TR930\|c0_g1_i1\|g.569 TR930\|c0_g1_i1\|m.569 type:complete len:438 (+) TR930\|c0_g1_i1:110-1423(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] TR930\|c0_g1_i3\|g.573 ORF TR930\|c0_g1_i3\|g.573 TR930\|c0_g1_i3\|m.573 type:complete len:438 (+) TR930\|c0_g1_i3:3138-4451(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] YHR174W SGDID:S000001217, Chr VIII from 451327-452640, Genome Release 64-1-1, Verified ORF, "Enolase II, a phosphopyruvate hydratase that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is induced in response to glucose" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_8076\|g.5841 ORF asmbl_8076\|g.5841 asmbl_8076\|m.5841 type:complete len:438 (+) asmbl_8076:3120-4433(+) [DBVPG6044_Trinity_v2_2_0_PASA_6May2016-contam.fasta] asmbl_8123\|g.6081 ORF asmbl_8123\|g.6081 asmbl_8123\|m.6081 type:complete len:438 (+) asmbl_8123:3137-4450(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_8280\|g.5102 ORF ID=asmbl_8280\|g.5102 ID=asmbl_8280\|m.5102 type:complete len:438 (+) ID=asmbl_8280:3121-4434(+) [DBVPG6044_quiverGenomes_Trinity_v2_2_0_PASA_2June2016-contam.fasta] ID=asmbl_8124\|g.4861 ORF ID=asmbl_8124\|g.4861 ID=asmbl_8124\|m.4861 type:complete len:438 (+) ID=asmbl_8124:3138-4451(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	437 amino acids
Gene Ontology:
Cellular Component:	soluble fraction [IDA] mitochondrion [IDA] phosphopyruvate hydratase complex [IDA] vacuole [IDA]
Biological Process:	gluconeogenesis [IEP] regulation of vacuole fusion, non-autophagic [IDA][IMP] glycolysis [IMP]
Molecular Function:	phosphopyruvate hydratase activity [IMP]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MAVSKVYARS VYDSRGNPTV EVELTTEKGV FRSIVPSGAS TGVHEALEMR DEDKSKWMGK  60
   61 GVMNAVNNVN NVIAAAFVKA NLDVKDQKAV DDFLLSLDGT ANKSKLGANA ILGVSMAAAR 120
  121 AAAAEKNVPL YQHLADLSKS KTSPYVLPVP FLNVLNGGSH AGGALALQEF MIAPTGAKTF 180
  181 AEAMRIGSEV YHNLKSLTKK RYGASAGNVG DEGGVAPNIQ TAEEALDLIV DAIKAAGHDG 240
  241 KVKIGLDCAS SEFFKDGKYD LDFKNPESDK SKWLTGVELA DMYHSLMKRY PIVSIEDPFA 300
  301 EDDWEAWSHF FKTAGIQIVA DDLTVTNPAR IATAIEKKAA DALLLKVNQI GTLSESIKAA 360
  361 QDSFAANWGV MVSHRSGETE DTFIADLVVG LRTGQIKTGA PARSERLAKL NQLLRIEEEL 420
  421 GDKAVYAGEN FHHGDKL

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ENO2 FBA1 GPM1 PGK1
View Details	Gavin AC, et al. (2006)		CHS1 ENO2 PGK1
View Details	Gavin AC, et al. (2002)		ACT1 ADA2 ADH1 AIM31 APL1 APL3 APL5 APL6 APM3 APM4 APS2 APS3 CCA1 CFT1 CFT2 CKA1 CKB1 CLP1 COG3 CSE2 ENO2 FAB1 FAR11 FAR3 FBA1 FIP1 FPR4 GAL11 GCN5 GLC7 GPM1 HFM1 HHF1, HHF2 HSC82 HTA1 MED1 MED11 MED2 MED4 MED6 MED7 MED8 MLP1 MPE1 MRPL6 NAN1 NUT1 NUT2 PAP1 PCF11 PDC1 PFK1 PFS2 PGD1 PGK1 POL5 PTA1 PTI1 PWP1 REF2 RGR1 RNA14 RNA15 ROM2 ROX3 RPG1 RPO21 RSA3 SEC13 SEC31 SIN4 SMC1 SNT2 SPF1 SPT7 SRB2 SRB4 SRB5 SRB6 SRB7 SRB8 SRP1 SSA3 SSE2 SSN2 SSN3 SSN8 SSU72 SYC1 TAF6 TIF1, TIF2 TIF4632 TPI1 TYE7 TYW1 VID24 VMR1 VPS53 YCL046W YIL077C YSH1 YTH1
View Details	Qiu et al. (2008)		ENO1 ENO2 LEU1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	TOR1	No Comments	Reinke A, et al. (2004)
View Run	BIR1	No Comments	Widlund PO, et al. (2005)
View Run	TOR1	No Comments	Reinke A, et al. (2004)
View Run	TOR2	No Comments	Reinke A, et al. (2004)
View Run	LST8	No Comments	Reinke A, et al. (2004)
View Run	TOR2	No Comments	Reinke A, et al. (2004)
View Run	KOG1	No Comments	Reinke A, et al. (2004)
View Run	CLB2	No Comments	McCusker D, et al (2007)
View Run	BIR1	No Comments	Widlund PO, et al. (2005)
View Run	BIR1	No Comments	Widlund PO, et al. (2005)
View Run	DAD1	Dam1 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	RPA135	his-HA tag on RPA135	Schneider, DA, et al. (2006)
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	MIH1	control 2	McCusker D, et al (2007)
View Run	MIH1	experimental2 - mih1-2	McCusker D, et al (2007)
View Run	ASF1	3912: TAP-tagged, strain background includes hpc2(delta)	Green EM, et al (2005)
View Run	RLF2	cac1: TAP-tagged	Green EM, et al (2005)
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	DAD1	Dam1 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	VAM3	sample: hx3	Hao Xu, et al. (2010)
View Run	MLP2	#19 Asynchronous Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#20 Asynchronous Prep1-TiO2 Phosphopeptide enrichment, PartB	Keck JM, et al. (2011)
View Run	MLP2	#25 Asynchronous Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#24 Asynchronous Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2	Keck JM, et al. (2011)
View Run	MLP2	#22 Asynchronous Prep2-IMAC Phosphopeptide enrichment B1-2	Keck JM, et al. (2011)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#31 Asynchronous Prep (Protease cleavage)	Keck JM, et al. (2011)
View Run	MLP2	#04 Alpha Factor Prep2	Keck JM, et al. (2011)
View Run	MLP2	#13 Mitotic Prep2-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#10 Mitotic Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#30 Asynchronous Prep6-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#28 Asynchronous Prep5-TiO2 Phosphopeptide Enrichment	Keck JM, et al. (2011)
View Run	MLP2	#06 Alpha Factor Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#08 Alpha Factor Prep4-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#19b Asynchronous Prep1-new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#05 Alpha Factor Prep2-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#07 Alpha Factor Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#09 Alpha Factor Prep4-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	None Entered	#17 Mitotic Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#19a Asynchronous Prep1-new search criteria	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

No yeast two-hybrid interactions found for this protein.

	PROTEIN(S)	PUBLICATION
View Data	ENO2	Huh WK, et al. (2003)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..127]	PSI-BLAST	11000.0	Enolase
2	View Details	[128..437]	PSI-BLAST	11000.0	Enolase

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.96

Protein Overview: ENO2

Protein Complex Data

Mass Spectrometry Data

Yeast Two-Hybrid Data

Microscopy / Localization Data

Protein Structure Data

Philius Transmembrane Prediction: