Protein Overview: SEH1

General Information:
Name(s) found:	SEH1 / YGL100W [SGD]
Description(s) found: Found 30 descriptions. SHOW ALL	Nuclear pore protein that is part of the evolutionarily conserved Nup84p complex (Nup84p, Nup85p, Nup120p, Nup145p, and Seh1p); homologous to Sec13p [SGD]
Organism:	Saccharomyces cerevisiae
Length:	349 amino acids
Gene Ontology:
Cellular Component:	nuclear pore [IDA]
Biological Process:	mRNA export from nucleus [TAS] rRNA export from nucleus [TAS] nuclear pore organization [TAS] snRNP protein import into nucleus [TAS] mRNA-binding (hnRNP) protein import into nucleus [TAS] snRNA export from nucleus [TAS] tRNA export from nucleus [TAS] NLS-bearing substrate import into nucleus [TAS] protein export from nucleus [TAS] ribosomal protein import into nucleus [TAS]
Molecular Function:	structural molecule activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MQPFDSGHDD LVHDVVYDFY GRHVATCSSD QHIKVFKLDK DTSNWELSDS WRAHDSSIVA  60
   61 IDWASPEYGR IIASASYDKT VKLWEEDPDQ EECSGRRWNK LCTLNDSKGS LYSVKFAPAH 120
  121 LGLKLACLGN DGILRLYDAL EPSDLRSWTL TSEMKVLSIP PANHLQSDFC LSWCPSRFSP 180
  181 EKLAVSALEQ AIIYQRGKDG KLHVAAKLPG HKSLIRSISW APSIGRWYQL IATGCKDGRI 240
  241 RIFKITEKLS PLASEESLTN SNMFDNSADV DMDAQGRSDS NTEEKAELQS NLQVELLSEH 300
  301 DDHNGEVWSV SWNLTGTILS SAGDDGKVRL WKATYSNEFK CMSVITAQQ

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		NUP120 NUP145 NUP84 NUP85 SEC13 SEH1
View Details	(MIPS) Mewes HW, et al. (2004)		NUP120 NUP145 NUP84 NUP85 SEC13 SEH1
View Details	Krogan NJ, et al. (2006)		MEX67 NUP120 NUP145 NUP84 NUP85 SEH1
View Details	Gavin AC, et al. (2006)		NUP120 NUP145 NUP84 NUP85 SEC13 SEH1
View Details	Gavin AC, et al. (2002)		CKA1 CKA2 CKB1 COP1 DBP2 ERO1 GLO3 GPT2 ILV1 MRPS28 NUP120 NUP145 NUP84 NUP85 OST1 OST6 PGK1 RET2 RET3 RPP2B SAM1 SCJ1 SEC13 SEC16 SEC21 SEC23 SEC26 SEC27 SEC28 SEC31 SEH1 STM1 STT3 SWP1 TIF34 TOS1 WBP1
View Details	Ho Y, et al. (2002)		ADE13 APE3 BMS1 CBP3 CCT6 CDC33 CDC53 CIC1 COF1 CPR6 CTF4 DBP10 DED81 DIA2 ENP1 FMP10 FPR3 HEM15 ILS1 KRE33 KRI1 LST4 MCM2 MCM3 MCM5 MDJ1 MTC5 NIP7 NMD3 NOP12 NPR2 NUP145 NUP84 NUP85 POX1 RNQ1 RRP12 SEC13 SEH1 SKP1 SLT2 SPB1 SSF1 SSF2 SSZ1 THI21 TIF6 URB1 UTP10 YAK1 YBL104C YHR033W YMR209C
View Details	Qiu et al. (2008)		MEX67 NDC1 NSP1 NUP116 NUP120 NUP133 NUP145 NUP188 NUP42 NUP49 NUP53 NUP57 NUP85 POM152 SEC13 SEH1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	VAM3	hx23: mixture in detergent (control) Investigating Vam3p, Nyv1p and their partners in trans-SNARE complex	Hao Xu, et al. (2010)
View Run	MLP2	#10 Mitotic Prep1-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	YJR141W	SEH1	1	Unpublished Fields Lab Data
View Screen	NAF1	SEH1	1	Hazbun TR, et al. (2003)
View Screen	APC5	SEH1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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No microscopy data found in the PDR for this protein.

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..349]	PSI-BLAST	95.612477	Tup1, C-terminal domain

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
binding	2.2778252170547	bayes_pls_golite062009
snoRNA binding	1.61553185503007	bayes_pls_golite062009
protein binding	1.24173112731149	bayes_pls_golite062009
transporter activity	1.21549336905926	bayes_pls_golite062009
protein transporter activity	0.964340340346761	bayes_pls_golite062009
substrate-specific transporter activity	0.734793983890004	bayes_pls_golite062009
nucleic acid binding	0.693814013902061	bayes_pls_golite062009
cytoskeletal protein binding	0.359536347486199	bayes_pls_golite062009
receptor activity	0.314900439093911	bayes_pls_golite062009
transcription regulator activity	0.190100099674138	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	0.168317261786777	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	0.132660456600365	bayes_pls_golite062009
kinase activity	0.127621956173205	bayes_pls_golite062009
	0.0248988265652406	bayes_pls_golite062009
nucleoside-triphosphatase activity	0.00585398682921312	bayes_pls_golite062009

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2