Protein Overview: NUP53

General Information:
Name(s) found:	NUP53 / YMR153W [SGD]
Description(s) found: Found 23 descriptions. SHOW ALL	Subunit of the nuclear pore complex (NPC), interacts with karyopherin Kap121p or with Nup170p via overlapping regions of Nup53p, involved in activation of the spindle checkpoint mediated by the Mad1p-Mad2p complex [SGD]
Organism:	Saccharomyces cerevisiae
Length:	475 amino acids
Gene Ontology:
Cellular Component:	nuclear pore [IDA]
Biological Process:	mRNA export from nucleus [TAS] rRNA export from nucleus [TAS] nuclear pore organization [TAS] snRNP protein import into nucleus [TAS] mRNA-binding (hnRNP) protein import into nucleus [TAS] snRNA export from nucleus [TAS] tRNA export from nucleus [TAS] NLS-bearing substrate import into nucleus [TAS] protein export from nucleus [TAS] protein import into nucleus, docking [IPI] ribosomal protein import into nucleus [TAS]
Molecular Function:	structural molecule activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MADLQKQENS SRFTNVSVIA PESQGQHEQQ KQQEQLEQQK QPTGLLKGLN GFPSAPQPLF  60
   61 MEDPPSTVSG ELNDNPAWFN NPRKRAIPNS IIKRSNGQSL SPVRSDSADV PAFSNSNGFN 120
  121 NVTFGSKKDP RILKNVSPND NNSANNNAHS SDLGTVVFDS NEAPPKTSLA DWQKEDGIFS 180
  181 SKTDNIEDPN LSSNITFDGK PTATPSPFRP LEKTSRILNF FDKNTKTTPN TASSEASAGS 240
  241 KEGASTNWDD HAIIIFGYPE TIANSIILHF ANFGEILEDF RVIKDFKKLN SKNMSKSPSL 300
  301 TAQKYPIYTG DGWVKLTYKS ELSKSRALQE NGIIMNGTLI GCVSYSPAAL KQLASLKKSE 360
  361 EIINNKTSSQ TSLSSKDLSN YRKTEGIFEK AKAKAVTSKV RNAEFKVSKN STSFKNPRRL 420
  421 EIKDGRSLFL RNRGKIHSGV LSSIESDLKK REQASKSKKS WLNRLNNWLF GWNDL

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Ho Y, et al. (2002)		BFA1 CDC15 CLU1 DUG2 FAA4 NUP53 RNR1 RNR2 SSD1 TEM1 YTM1
View Details	Qiu et al. (2008)		ASM4 GLE2 NDC1 NSP1 NUP1 NUP100 NUP116 NUP133 NUP145 NUP157 NUP159 NUP170 NUP188 NUP192 NUP42 NUP49 NUP53 NUP57 NUP82 POM152 SEC13 SEH1 SUS1

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	INP54	NUP53	2	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	NUP53	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..379]	ab initio	12.52	No description for 1i3qA was found.
2	View Details	[380..475]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.95

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2