Protein Overview: NOP58

General Information:
Name(s) found:	NOP58 / YOR310C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|940841\|emb\|CAA62165.1\| orf 06108 [Saccharomyces cerevisiae] [NCBI NR] gi\|6324886\|ref\|NP_014955.1\| Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA; Nop58p [Saccharomyces cerevisiae] [NCBI NR] gi\|3170533\|gb\|AAC39484.1\| nucleolar protein Nop5p [Saccharomyces cerevisiae] [NCBI NR] gi\|2833225\|sp\|Q12499.1\|NOP58_YEAST RecName: Full=Nucleolar protein 58; AltName: Full=Nucleolar protein 5 [NCBI NR] pir\|\|S58322 nucleolar protein NOP5 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|1420682\|emb\|CAA99630.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] (Q12499) Nucleolar protein NOP58 (Nucleolar protein NOP5) [Swiss-Prot] Nucleolar protein 58 OS=Saccharomyces cerevisiae GN=NOP58 PE=1 SV=1 [Swiss-Prot] NOP58 Protein required for pre-18S rRNA processing [mips] Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA [SGD] Chr XV from 898353-896818, reverse complement [hmr_yeast5_DM3-1_pombe.fasta] Chr XV from 896817-898352, reverse complement [yeastorf3.fasta] Chr XV from 896818-898353, reverse complement [yeastorf4.fasta] NOP58, Chr XV from 896817-898352, reverse complement [yeast_orfs2] NOP58 SGDID:S0005837, Chr XV from 898355-896820, reverse complement, Verified ORF [yeastflysequest.fasta] Nop58p [Saccharomyces cerevisiae]gi\|1420682\|emb\|CAA99630.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|940841\|emb\|CAA62165.1\| orf 06108 [Saccharomyces cerevisiae]gi\|3170533\|gb\|AAC39484.1\| nucleolar protein Nop5p [Saccharomyces cerevisiae]gi\| [nrdb_11152004_Chlamydomonas] NOP58 SGDID:S000005837, Chr XV from 898355-896820, reverse complement, Verified ORF, "Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] NOP58 SGDID:S000005837, Chr XV from 898357-896822, reverse complement, Verified ORF, "Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of [yeast-contam.fasta] Nucleolar protein NOP58 (Nucleolar protein NOP5) [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] orf 06108 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA; Nop58p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] nucleolar protein Nop5p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] NOP58 SGDID:S000005837, Chr XV from 898357-896822, reverse complement, Verified ORF, "Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA" [yeast-bovrabprots.fasta] [chemostatdb.fasta] Nucleolar protein 58 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=NOP58 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] NOP58 SGDID:S000005837, Chr XV from 898360-896825, Genome Release 64-1-1, reverse complement, Verified ORF, "Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA" [yeast-030211-contam.fasta] NOP58 SGDID:S000005837, Chr XV from 898360-896825, Genome Release 64-2-1, reverse complement, Verified ORF, "Protein involved in producing mature rRNAs and snoRNAs; involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA" [20141208_orf_trans.fasta] YOR310C SGDID:S000005837, Chr XV from 898360-896825, Genome Release 64-1-1, reverse complement, Verified ORF, "Protein involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	511 amino acids
Gene Ontology:
Cellular Component:	box C/D snoRNP complex [IDA] nuclear outer membrane [TAS] small nucleolar ribonucleoprotein complex [IPI]
Biological Process:	rRNA modification [TAS] maturation of SSU-rRNA [IPI] ribosome biogenesis [RCA]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MAYVLTETSA GYALLKASDK KIYKSSSLIQ DLDSSDKVLK EFKIAAFSKF NSAANALEEA  60
   61 NSIIEGKVSS QLEKLLEEIK KDKKSTLIVS ETKLANAINK LGLNFNVVSD AVTLDIYRAI 120
  121 KEYLPELLPG MSDNDLSKMS LGLAHSIGRH KLKFSADKVD VMIIQAIALL DDLDKELNTY 180
  181 AMRCKEWYGW HFPELAKIVT DSVAYARIIL TMGIRSKASE TDLSEILPEE IEERVKTAAE 240
  241 VSMGTEITQT DLDNINALAE QIVEFAAYRE QLSNYLSARM KAIAPNLTQL VGELVGARLI 300
  301 AHSGSLISLA KSPASTIQIL GAEKALFRAL KTKHDTPKYG LLYHASLVGQ ATGKNKGKIA 360
  361 RVLAAKAAVS LRYDALAEDR DDSGDIGLES RAKVENRLSQ LEGRDLRTTP KVVREAKKVE 420
  421 MTEARAYNAD ADTAKAASDS ESDSDDEEEE KKEKKEKKRK RDDDEDSKDS KKAKKEKKDK 480
  481 KEKKEKKEKK EKKEKKEKKE KKSKKEKKEK K

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		BUD21 CBF5 GAR1 HCA4 KRE33 KRI1 KRR1 LUG1 MPP10 NOP14 NOP56 NOP58 PRP43 RPA49 RPF2 RPL6A RRP5 RRP9 SNU13 SRO9 UTP22
View Details	Hazbun TR, et al. (2003)		BFR2 ENP2 ESF1 HCA4 LCP5 NOP58 UTP9
View Details	(MIPS) Mewes HW, et al. (2004)		NOP1 NOP56 NOP58
View Details	Krogan NJ, et al. (2006)		BUD21 GLK1 NOP58 PRP4 RRP9
View Details	Gavin AC, et al. (2006)		CMS1 DIP2 MRD1 NAN1 NOP58 PNO1 PWP2 ROK1 RRP9 UTP10 UTP13 UTP15 UTP18 UTP21 UTP22 UTP4 UTP6 UTP7 UTP8 UTP9
View Details	Gavin AC, et al. (2002)		ADE4 ASC1 BFR2 BMS1 BRX1 BUD21 CBF5 CFT2 CKA1 CKA2 CKB1 CKB2 CMS1 DBP8 DIA4 DIM1 DIP2 DIS3 ECM16 ECM29 EMG1 ENP1 ENP2 ERO1 ESF1 ESF2 FAP7 FIP1 GCN1 GLC7 HAS1 HCA4 HHF1, HHF2 HSL1 HTA2 IMP3 IMP4 KRE33 KRR1 LCP5 LTV1 MPP10 MRD1 MVD1 NAN1 NOB1 NOC2 NOC4 NOP1 NOP12 NOP14 NOP56 NOP58 NOP6 PAP1 PFS2 PNO1 PRE6 PRE9 PRP43 PTA1 PTI1 PWP2 RCL1 REF2 RML2 RNA14 ROK1 RPP2B RRP12 RRP7 RRP9 RTG2 SAM1 SCL1 SEC21 SLX9 SOF1 SRO9 TIF1, TIF2 TSR1 UTP10 UTP11 UTP13 UTP15 UTP18 UTP20 UTP21 UTP22 UTP30 UTP4 UTP6 UTP7 UTP8 UTP9 YOR059C YSH1
View Details	Ho Y, et al. (2002)		AVO1 CBF5 CBP2 CIC1 DBP7 DRS1 EBP2 ENP2 ERB1 HTB1 KAP95 KRE33 KRI1 KRR1 MAK5 MNP1 MSS116 NOC2 NOP2 NOP58 NOP6 NOP7 NUG1 PRO1 PUF6 PWP1 RPF2 RRP9 RRT6 TIF1, TIF2 TIF4631 TIF6 TSR1 UTP20

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	NAF1	No Comments	Hazbun TR, et al. (2003)
View Run	ESF1	No Comments	Hazbun TR, et al. (2003)
View Run	NOP9	No Comments	Hazbun TR, et al. (2003)
View Run	ESF1	No Comments	Hazbun TR, et al. (2003)
View Run	MIA40	No Comments	Hazbun TR, et al. (2003)
View Run	LST8	No Comments	Reinke A, et al. (2004)
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	MIH1	control 1	McCusker D, et al (2007)
View Run	CEP3	sample cbf3 from feb 2005	Sandall S, et all (2006)
View Run	RLF2	cac1: TAP-tagged	Green EM, et al (2005)
View Run	DAD1	Dam1-765 complex purified from yeast with Dad1-TAP	Shimogawa MM, et al. (2006)
View Run	RPA135	No Comments	Schneider, DA, et al. (2006)
View Run	ZDS1	Sample 2- cpn2 from june 2005	McCusker D, et al (2007)
View Run	ZDS1	Sample 3 - cpn + atp, from june 2005	McCusker D, et al (2007)
View Run	BOI1	Sample bob1 (2nd set) from october 2005	McCusker D, et al (2007)
View Run	VAM3	Sample: HX12 - trans-SNARE complex formation and fusion are inhibited (control).	Hao Xu, et al. (2010)
View Run	VAM3	Sample: HX14 - mixture in detergent (control).	Hao Xu, et al. (2010)
View Run	MLP2	#20 Asynchronous Prep1-TiO2 Phosphopeptide enrichment, PartB	Keck JM, et al. (2011)
View Run	MLP2	#26 Asynchronous Prep4-TiO2 Phosphopeptide enriched, Steps1-2	Keck JM, et al. (2011)
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#31 Asynchronous Prep (Protease cleavage)	Keck JM, et al. (2011)
View Run	MLP2	#03 Alpha Factor Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#12 Mitotic Prep1-TiO2 Flowthrough	Keck JM, et al. (2011)
View Run	MLP2	#02 Alpha Factor Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#11 Mitotic Prep1-TiO2 enriched, new search criteria	Keck JM, et al. (2011)
View Run	MLP2	#06 Alpha Factor Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#08 Alpha Factor Prep4-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	None Entered	#17 Mitotic Prep3-TiO2 Phosphopeptide enrichment	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	UTP5	NOP58	1	Unpublished Fields Lab Data
View Screen	CHL4	NOP58	1	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	NOP58	Huh WK, et al. (2003)
View Data	NOP58 and NOP56	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..383]	ORFEUS	8.68	(+)-bornyl diphosphate synthase
2	View Details	[384..511]	PSI-BLAST	3.0	Heavy meromyosin subfragment

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
transferase activity	2.60637425996067	bayes_pls_golite062009
catalytic activity	2.20533251370971	bayes_pls_golite062009
prenyltransferase activity	1.66806975285604	bayes_pls_golite062009
protein geranylgeranyltransferase activity	1.19735677334163	bayes_pls_golite062009
transferase activity, transferring alkyl or aryl (other than methyl) groups	0.765111353419857	bayes_pls_golite062009
protein binding	0.669412419580008	bayes_pls_golite062009
binding	0.61846029601398	bayes_pls_golite062009
intramolecular transferase activity	0.467423278732604	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.99

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2