Protein Overview: SPB4

General Information:
Name(s) found:	SPB4 / YFL002C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|836754\|dbj\|BAA09238.1\| rRNA helicase [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S14942 RNA helicase SPB4 - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|4522\|emb\|CAA34272.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] gi\|14318521\|ref\|NP_116654.1\| Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients; Spb4p [Saccharomyces cerevisiae] [NCBI NR] gi\|134787\|sp\|P25808.1\|SPB4_YEAST RecName: Full=ATP-dependent rRNA helicase SPB4; AltName: Full=Suppressor of PAB1 protein 4 [NCBI NR] ATP-dependent rRNA helicase SPB4 OS=Saccharomyces cerevisiae GN=SPB4 PE=1 SV=1 [Swiss-Prot] SPB4 ATP-dependent RNA helicase of DEAH box family [mips] Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients [SGD] SPB4, Chr VI from 145108-146928, reverse complement [yeast_orfs2] Chr VI from 145108-146928, reverse complement [yeastorf3.fasta] Chr VI from 146929-145109, reverse complement [yeastorf5.fasta] (P25808) ATP-dependent rRNA helicase SPB4 [4932.FASTACCERV.txt] SPB4 SGDID:S0001894, Chr VI from 146929-145109, reverse complement, Verified ORF [yeastflysequest.fasta] Spb4p [Saccharomyces cerevisiae]gi\|4522\|emb\|CAA34272.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|134787\|sp\|P25808\|SPB4_YEAST ATP-dependent rRNA helicase SPB4gi\|83511\|pir\|\|S14942 RNA helicase SPB4 - yeast (Saccharomyces cerevisiae)gi\|836754 [nrdb_11152004_Chlamydomonas] SPB4 SGDID:S000001894, Chr VI from 146929-145109, reverse complement, Verified ORF, "Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] rRNA helicase [Saccharomyces cerevisiae] [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] ATP-dependent rRNA helicase SPB4 (Suppressor of PAB1 protein 4) [nr-271106-contam.fasta] Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients; Spb4p [Saccharomyces cerevisiae] [nr-271106-contam.fasta] SPB4 SGDID:S000001894, Chr VI from 146929-145109, reverse complement, Verified ORF, "Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients" [yeast-bovrabprots.fasta] [chemostatdb.fasta] ATP-dependent rRNA helicase SPB4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=SPB4 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] SPB4 SGDID:S000001894, Chr VI from 146934-145114, Genome Release 64-1-1, reverse complement, Verified ORF, "Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients" [yeast-030211-contam.fasta] SPB4 SGDID:S000001894, Chr VI from 146934-145114, Genome Release 64-2-1, reverse complement, Verified ORF, "Putative ATP-dependent RNA helicase; nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients" [20141208_orf_trans.fasta] TR8017\|c0_g1_i1\|g.5565 ORF TR8017\|c0_g1_i1\|g.5565 TR8017\|c0_g1_i1\|m.5565 type:complete len:607 (+) TR8017\|c0_g1_i1:79-1899(+) [Y12_S_cerevisiae-Sep2015-contam.fasta] YFL002C SGDID:S000001894, Chr VI from 146934-145114, Genome Release 64-1-1, reverse complement, Verified ORF, "Putative ATP-dependent RNA helicase, nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta] asmbl_9488\|g.6860 ORF asmbl_9488\|g.6860 asmbl_9488\|m.6860 type:complete len:607 (+) asmbl_9488:60-1880(+) [Y12_Trinity_v2_2_0_PASA_6May2016-contam.fasta] ID=asmbl_9495\|g.5716 ORF ID=asmbl_9495\|g.5716 ID=asmbl_9495\|m.5716 type:complete len:607 (+) ID=asmbl_9495:60-1880(+) [Y12_quiver_Trinity_v2_2_0_PASA_2June2016-contam.fasta]
Organism:	Saccharomyces cerevisiae
Length:	606 amino acids
Gene Ontology:
Cellular Component:	nuclear outer membrane [TAS] nucleolus [TAS]
Biological Process:	ribosome biogenesis [RCA] ribosomal large subunit assembly [TAS]
Molecular Function:	ATP-dependent RNA helicase activity [TAS]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSKSLEWDNL GFSLLPWIRT GLDVMGFETM TPVQASTIPM LAGNKDVVVD SVTGSGKTAA  60
   61 FVIPVLEKVV KEEANTSKFK KAHFHSLIIA PTRELSRQIE SVVLSFLEHY PSDLFPIKCQ 120
  121 LLVGTNEATV RDDVSNFLRN RPQILIGTPG RVLDFLQMPA VKTSACSMVV MDEADRLLDM 180
  181 SFIKDTEKIL RLLPKQRRTG LFSATMRSAG SDIFKTGLRN PVRITVNSKN QAPSSLKLNY 240
  241 CVVNPAEKLQ LLVSILNNYK FKKCIVYFPT CVSVSYFYSF IQYLGKRNIL VNEVEIFSLH 300
  301 GKLQTSARTK TLTAFTDSLS NSVLFTTDVA ARGIDIPDVD LVIQLDPPTN TDMFMHRCGR 360
  361 TGRANRVGKA ITFLNEGREE DFIPFMQVKN VELEELDLEV KGITTNFYED FRNWILEDRD 420
  421 RFDKGVKAYV AFIKYYSNHS ATSIFRLQSL DYVGIAKLYG LFRLPRMPEI TKYLATEKQE 480
  481 GIFPGNWLVD PPVNMDEYKY KDKKREKERQ ETLKNISLIN DKKKLKSELK KKNLAWSDKT 540
  541 LTKERKLERK EKMSLKRKAI EEELKAEELD ENAEEERIKE DWKEIVLQNK RKKVSSKAIQ 600
  601 GNFDDL

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		BRX1 CIC1 DBP10 DRS1 ERB1 FPR4 HAS1 MAK21 MRT4 NIP7 NOC2 NOC3 NOG1 NOP15 NOP2 NOP7 NSA1 NUG1 PUF6 RIX1 RIX7 RLP7 RPF1 RPL25 RRP1 SPB4 TIF6 YTM1
View Details	Krogan NJ, et al. (2006)		FCY21 MTG1 SPB4 YBL071C
View Details	Gavin AC, et al. (2006)		BRX1 CIC1 MAK21 NOC2 NOP4 SPB4 YTM1
View Details	Gavin AC, et al. (2002)		ARX1 BEM2 BMS1 BRX1 BUD20 CIC1 CLU1 CYR1 CYS4 DBP10 DBP9 DCP2 DRS1 EBP2 ERB1 FPR4 GCN1 HAS1 HDA1 HER1 HIS3 INP2 IPI1 IPI3 KRE33 LHP1 LOC1 LSG1 MAK21 MAK5 MDN1 MIS1 MRT4 NAP1 NIP1 NIP7 NMD3 NOC2 NOC3 NOG1 NOG2 NOP1 NOP12 NOP15 NOP16 NOP2 NOP4 NOP56 NOP7 NSA1 NSA2 NUG1 PRT1 PUF6 REI1 RIX1 RIX7 RLP24 RLP7 RPA135 RPA190 RPF1 RPF2 RPG1 RPP2B RPT6 RRP1 RRP12 RSA3 RSA4 RTR1 SDA1 SEC7 SKI2 SNU13 SPB1 SPB4 SSF1 TIF6 TUB3 URB1 UTP10 UTP20 UTP22 YGL036W YTM1
View Details	Ho Y, et al. (2002)		ACO1 CCT6 CDC14 EGD2 ERB1 GND1 GUS1 HAS1 HXT7 MET6 MRT4 MUB1 NOC2 NOC3 NOG1 NSA1 NUG1 PRP43 SAH1 SCS2 SEC53 SPB4 SSQ1 TIF6 UBR2 YTM1
View Details	Qiu et al. (2008)		BFR2 DBP10 DBP3 DBP6 DBP7 DBP8 DBP9 DHR2 DRS1 FAL1 FCF2 GAR1 HCA4 KRR1 MAK5 MTR4 NOC3 NOG2 NOP12 NOP13 NOP15 NOP4 NOP56 NOP7 NSR1 NUG1 PRP43 PXR1 RNT1 ROK1 RRP3 SPB4 UTP10 UTP13 UTP15 UTP4 UTP5 UTP6 UTP8 UTP9 YBL028C

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#25 Asynchronous Prep3-TiO2 Flowthrough	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

NOT SHOWING SINGLE HITS. [ Show Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	SPP382	SPB4	2	Hazbun TR, et al. (2003)

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	SPB4 and NOP56	Huh WK, et al. (2003)
View Data	SPB4	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..231]	PSI-BLAST	431.0	Putative DEAD box RNA helicase
2	View Details	[232..388]	PSI-BLAST	431.0	Putative DEAD box RNA helicase
3	View Details	[389..519]	PSI-BLAST	10.0	Nucleotide excision repair enzyme UvrB
4	View Details	[520..606]	deduced	N/A	No confident structure predictions are available.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.92

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2