Protein Overview: DUN1

General Information:
Name(s) found:	DUN1 / YDL101C [SGD]
Description(s) found: SHOW ONLY BEST	gi\|729375\|sp\|P39009.1\|DUN1_YEAST RecName: Full=DNA damage response protein kinase DUN1 [NCBI NR] gi\|6320102\|ref\|NP_010182.1\| Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicativ [NCBI NR] pir\|\|S43941 protein kinase DUN1 (EC 2.7.1.-) - yeast (Saccharomyces cerevisiae) [NCBI NR] gi\|435617\|gb\|AAA16324.1\| protein kinase [Saccharomyces cerevisiae] [NCBI NR] gi\|1431140\|emb\|CAA98668.1\| DUN1 [Saccharomyces cerevisiae] [NCBI NR] gi\|1199548\|emb\|CAA64912.1\| DUN1 [Saccharomyces cerevisiae] [NCBI NR] DNA damage response protein kinase DUN1 OS=Saccharomyces cerevisiae GN=DUN1 PE=1 SV=1 [Swiss-Prot] DUN1 Protein kinase [mips] Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair [SGD] DUN1, Chr IV from 280306-281847, reverse complement [yeast_orfs2] Chr IV from 280306-281847, reverse complement [yeastorf3.fasta] Chr IV from 281847-280306, reverse complement [yeastorf5.fasta] (P39009) DNA damage response protein kinase DUN1 (EC 2.7.1.-) [4932.FASTACCERV.txt] DUN1 SGDID:S0002259, Chr IV from 281848-280307, reverse complement, Verified ORF [yeastflysequest.fasta] Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair [Saccharomyces [nrdb_11152004_Chlamydomonas] (P39009) DNA damage response protein kinase DUN1 (EC 2.7.1.37) [cerv4932.FASTAC] DUN1 SGDID:S000002259, Chr IV from 281848-280307, reverse complement, Verified ORF, "Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2 [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] protein kinase [nr-271106-contam.fasta] DUN1 [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair; Dun1p [Sacchar [nr-271106-contam.fasta] DNA damage response protein kinase DUN1 [nr-271106-contam.fasta] DUN1 SGDID:S000002259, Chr IV from 281848-280307, reverse complement, Verified ORF, "Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair" [yeast-bovrabprots.fasta] [chemostatdb.fasta] DNA damage response protein kinase DUN1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=DUN1 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] DUN1 SGDID:S000002259, Chr IV from 281848-280307, Genome Release 64-1-1, reverse complement, Verified ORF, "Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair" [yeast-030211-contam.fasta] DUN1 SGDID:S000002259, Chr IV from 281848-280307, Genome Release 64-2-1, reverse complement, Verified ORF, "Cell-cycle checkpoint serine-threonine kinase; required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; Mec1p and Dun1p function in same pathway to regulate both dNTP pools and telomere length; also regulates postreplicative DNA repair" [20141208_orf_trans.fasta] YDL101C SGDID:S000002259, Chr IV from 281848-280307, Genome Release 64-1-1, reverse complement, Verified ORF, "Cell-cycle checkpoint serine-threonine kinase required for DNA damage-induced transcription of certain target genes, phosphorylation of Rad55p and Sml1p, and transient G2/M arrest after DNA damage; also regulates postreplicative DNA repair" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	513 amino acids
Gene Ontology:
Cellular Component:	nucleus [IDA]
Biological Process:	DNA damage checkpoint [IMP] cell cycle checkpoint [IMP] telomere maintenance [IMP] protein amino acid phosphorylation [IDA]
Molecular Function:	protein kinase activity [IDA]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSLSTKREHS GDVTDSSFKR QQRSNKPSSE YTCLGHLVNL IPGKEQKVEI TNRNVTTIGR  60
   61 SRSCDVILSE PDISTFHAEF HLLQMDVDNF QRNLINVIDK SRNGTFINGN RLVKKDYILK 120
  121 NGDRIVFGKS CSFLFKYASS SSTDIENDDE KVSSESRSYK NDDEVFKKPQ ISATSSQNAT 180
  181 TSAAIRKLNK TRPVSFFDKY LLGKELGAGH YALVKEAKNK KTGQQVAVKI FHAQQNDDQK 240
  241 KNKQFREETN ILMRVQHPNI VNLLDSFVEP ISKSQIQKYL VLEKIDDGEL FERIVRKTCL 300
  301 RQDESKALFK QLLTGLKYLH EQNIIHRDIK PENILLNITR RENPSQVQLG PWDEDEIDIQ 360
  361 VKIADFGLAK FTGEMQFTNT LCGTPSYVAP EVLTKKGYTS KVDLWSAGVI LYVCLCGFPP 420
  421 FSDQLGPPSL KEQILQAKYA FYSPYWDKID DSVLHLISNL LVLNPDERYN IDEALNHPWF 480
  481 NDIQQQSSVS LELQRLQITD NKIPKTYSEL SCL

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Ho Y, et al. (2002)		AAC3 ADH4 AGX1 AHA1 AHP1 ATP3 BUL1 CAR2 CCT2 CCT3 CCT5 CDC33 CKA2 CKB1 COR1 CPR1 DMA2 DUN1 EGD1 FAR1 GCD11 GPD1 GPD2 GPH1 HHT1, HHT2 HOM2 HOR2 HTB1 LSP1 MDM30 MGT1 MRE11 NAP1 NAT1 NMD3 PDC6 PDX3 PFK1 PGM2 PHO84 PIL1 PRP19 QCR2 RAD1 RAD24 RAD28 RAD50 RAD53 RAD9 REX2 RFC2 RFC3 RFC5 RNH203 RNQ1 RPT3 RSP5 SAW1 SEC23 SEC27 SEC53 SEC6 SEN1 SML1 SOD1 SRS2 STE20 TCP1 TFP1 TIF1, TIF2 TMA29 TOP2 UBI4 VMA4 XRS2 YHR033W YJU2 YLR413W YNK1

Mass Spectrometry Data

No mass spectrometry results found for this protein.

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	TOP3	DUN1	2	Unpublished Fields Lab Data
View Screen	SGS1	DUN1	2	Unpublished Fields Lab Data
View Screen	DUN1	KTR3	2	Unpublished Fields Lab Data
View Screen	DUN1	DET1	2	Unpublished Fields Lab Data
View Screen	DUN1	KAR9	2	Unpublished Fields Lab Data
View Screen	YCK1	DUN1	2	Uetz P, et al. (2000)
View Screen	CTR86	DUN1	1	Unpublished Fields Lab Data
View Screen	DUN1	RPS6A, RPS6B	1	Unpublished Fields Lab Data
View Screen	DUN1	SEC31	1	Unpublished Fields Lab Data
View Screen	DUN1	RAD51	1	Unpublished Fields Lab Data
View Screen	DUN1	SHO1	1	Unpublished Fields Lab Data
View Screen	PRP16	DUN1	1	Unpublished Fields Lab Data

Microscopy / Localization Data

New Feature: Upload Your Own Microscopy Data

	PROTEIN(S)	PUBLICATION
View Data	DUN1	Huh WK, et al. (2003)

Protein Structure Data

[What does the above image mean?]
[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..152]	PSI-BLAST	22.39794	Chk2 kinase
2	View Details	[153..287] [501..513]	PSI-BLAST	636.457575	Calmodulin-dependent protein kinase
3	View Details	[288..500]	PSI-BLAST	636.457575	Calmodulin-dependent protein kinase

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
binding	3.6561903894619	bayes_pls_golite062009
protein binding	3.44553762357492	bayes_pls_golite062009
transcription regulator activity	3.23926962992034	bayes_pls_golite062009
DNA binding	3.12131448113144	bayes_pls_golite062009
nucleic acid binding	2.91028042727128	bayes_pls_golite062009
histone binding	2.79033314021304	bayes_pls_golite062009
transforming growth factor beta receptor, cytoplasmic mediator activity	2.78221661189523	bayes_pls_golite062009
transmembrane receptor protein serine/threonine kinase signaling protein activity	2.56188495627199	bayes_pls_golite062009
receptor signaling protein activity	2.25132862320271	bayes_pls_golite062009
transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity	2.06369312345519	bayes_pls_golite062009
structure-specific DNA binding	1.95921851331594	bayes_pls_golite062009
transcription factor activity	1.89464382469326	bayes_pls_golite062009
phosphotransferase activity, alcohol group as acceptor	1.88096476099716	bayes_pls_golite062009
double-stranded DNA binding	1.84746485315093	bayes_pls_golite062009
kinase activity	1.82476774968305	bayes_pls_golite062009
sequence-specific DNA binding	1.78280248668052	bayes_pls_golite062009
DNA replication origin binding	1.78246425241581	bayes_pls_golite062009
protein kinase activity	1.73373132141588	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	1.66058356077795	bayes_pls_golite062009
SMAD binding	1.58868248655216	bayes_pls_golite062009
type I transforming growth factor beta receptor binding	1.58437603150322	bayes_pls_golite062009
transcription repressor activity	1.5642245057124	bayes_pls_golite062009
transcription activator activity	1.53960544319629	bayes_pls_golite062009
RNA polymerase II transcription factor activity	1.48483625404671	bayes_pls_golite062009
promoter binding	1.34105825186678	bayes_pls_golite062009
transferase activity	1.29860325699399	bayes_pls_golite062009
protein serine/threonine kinase activity	1.1868962075754	bayes_pls_golite062009
transcription factor binding	0.93544177518754	bayes_pls_golite062009
small conjugating protein ligase activity	0.9108432673413	bayes_pls_golite062009
ubiquitin-protein ligase activity	0.90981150861416	bayes_pls_golite062009
molecular transducer activity	0.767071864136971	bayes_pls_golite062009
signal transducer activity	0.767071864136971	bayes_pls_golite062009
R-SMAD binding	0.70816286022176	bayes_pls_golite062009
chromatin binding	0.62935034847038	bayes_pls_golite062009
transmembrane receptor protein serine/threonine kinase binding	0.459063012088486	bayes_pls_golite062009
transforming growth factor beta receptor binding	0.459063012088486	bayes_pls_golite062009
enzyme binding	0.34998623523914	bayes_pls_golite062009
protein dimerization activity	0.33340925100096	bayes_pls_golite062009
co-SMAD binding	0.325865547214071	bayes_pls_golite062009
damaged DNA binding	0.279328480488455	bayes_pls_golite062009
collagen binding	0.241950844569571	bayes_pls_golite062009
receptor serine/threonine kinase binding	0.121846363304069	bayes_pls_golite062009
receptor binding	0.0581553668794451	bayes_pls_golite062009
identical protein binding	0.0513801649373811	bayes_pls_golite062009
protein homodimerization activity	0.0339057097360133	bayes_pls_golite062009

Term	Confidence	Notes
phosphotransferase activity, alcohol group as acceptor	5.49988294224815	bayes_pls_golite062009
protein kinase activity	5.4586557943719	bayes_pls_golite062009
kinase activity	5.3287080791198	bayes_pls_golite062009
transferase activity, transferring phosphorus-containing groups	4.96052051118534	bayes_pls_golite062009
protein serine/threonine kinase activity	4.46381887831481	bayes_pls_golite062009
calmodulin-dependent protein kinase activity	4.31938467136214	bayes_pls_golite062009
transferase activity	3.97138602499442	bayes_pls_golite062009
binding	2.74863538127007	bayes_pls_golite062009
actin binding	2.37384162366989	bayes_pls_golite062009
protein binding	2.276111749106	bayes_pls_golite062009
telethonin binding	2.27062712605521	bayes_pls_golite062009
tubulin binding	2.18641675163967	bayes_pls_golite062009
cytoskeletal protein binding	2.12615078788254	bayes_pls_golite062009
ATP binding	1.97600611746091	bayes_pls_golite062009
adenyl ribonucleotide binding	1.93596671856664	bayes_pls_golite062009
adenyl nucleotide binding	1.90928021591891	bayes_pls_golite062009
microtubule binding	1.88110801192607	bayes_pls_golite062009
purine ribonucleotide binding	1.65732943334212	bayes_pls_golite062009
ribonucleotide binding	1.65726906484847	bayes_pls_golite062009
purine nucleotide binding	1.63763394711025	bayes_pls_golite062009
nucleotide binding	1.63195828048121	bayes_pls_golite062009
insulin receptor substrate binding	1.62657794525303	bayes_pls_golite062009
protein tyrosine kinase activity	1.3898059094397	bayes_pls_golite062009
alpha-actinin binding	1.38861866085902	bayes_pls_golite062009
receptor signaling protein serine/threonine kinase activity	1.38496328649031	bayes_pls_golite062009
transcription regulator activity	1.30988141770642	bayes_pls_golite062009
gamma-tubulin binding	1.28511591054866	bayes_pls_golite062009
receptor signaling protein activity	1.27291233344322	bayes_pls_golite062009
catalytic activity	1.21655087018101	bayes_pls_golite062009
actin filament binding	1.20555646820348	bayes_pls_golite062009
DNA binding	1.17130513163489	bayes_pls_golite062009
nucleic acid binding	1.16049201286103	bayes_pls_golite062009
transcription repressor activity	0.959888295228949	bayes_pls_golite062009
cyclin-dependent protein kinase activity	0.959360414036299	bayes_pls_golite062009
transcription factor activity	0.919430701010326	bayes_pls_golite062009
MAP kinase activity	0.81943579965027	bayes_pls_golite062009
structural constituent of cytoskeleton	0.612883428421652	bayes_pls_golite062009
transporter activity	0.553953601841147	bayes_pls_golite062009
general RNA polymerase II transcription factor activity	0.51726292539973	bayes_pls_golite062009
protein serine/threonine/tyrosine kinase activity	0.48345772948903	bayes_pls_golite062009
enzyme binding	0.46284745266176	bayes_pls_golite062009
magnesium ion binding	0.393328812780268	bayes_pls_golite062009
transmembrane transporter activity	0.37676337669549	bayes_pls_golite062009
MAP kinase kinase kinase activity	0.37115026509016	bayes_pls_golite062009
MAP kinase kinase activity	0.35402045013148	bayes_pls_golite062009
protein complex binding	0.343240719858581	bayes_pls_golite062009
structure-specific DNA binding	0.318153468064373	bayes_pls_golite062009
signal transducer activity	0.270054246024761	bayes_pls_golite062009
molecular transducer activity	0.270054246024761	bayes_pls_golite062009
substrate-specific transporter activity	0.0611721261859464	bayes_pls_golite062009
transmembrane receptor protein kinase activity	0.0556163711947139	bayes_pls_golite062009
transmembrane receptor activity	0.00652065412910241	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.96

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2