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Protein Overview: DPB2

General Information:
Name(s) found: DPB2 / YPR175W [SGD]
Description(s) found:
Found 18 descriptions.
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  • Second largest subunit of DNA polymerase II (DNA polymerase epsilon), required for normal yeast chromosomal replication; expression peaks at the G1/S phase boundary; potential Cdc28p substrate [SGD]
    • Organism: Saccharomyces cerevisiae
    Length: 689 amino acids

    Gene Ontology:
    Cellular Component: replication fork [TASLink to PubMed article]
    nucleus [IDALink to PubMed article]
    cytoplasm [IDALink to PubMed article]
    epsilon DNA polymerase complex [IDALink to PubMed article]
    Biological Process: lagging strand elongation [TASLink to PubMed article]
    leading strand elongation [TASLink to PubMed article]
    nucleotide-excision repair [TASLink to PubMed article]
    mismatch repair [TASLink to PubMed article]
    Molecular Function: DNA binding [IEA]
    nucleotidyltransferase activity [IEA]
    DNA-directed DNA polymerase activity [IEA][TAS]
    double-stranded DNA binding [IDA]
    transferase activity [IEA]


    Sequence:
    Sequence:
    [PDR BLAST]
    [ProtParam] 
          1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MFGSGNVLPV KIQPPLLRPL AYRVLSRKYG LSIKSDGLSA LAEFVGTNIG ANWRQGPATI  60
   61 KFLEQFAAVW KQQERGLFID QSGVKEVIQE MKEREKVEWS HEHPIQHEEN ILGRTDDDEN 120
  121 NSDDEMPIAA DSSLQNVSLS SPMRQPTERD EYKQPFKPES SKALDWRDYF KVINASQQQR 180
  181 FSYNPHKMQF IFVPNKKQNG LGGIAGFLPD IEDKVQMFLT RYYLTNDRVM RNENFQNSDM 240
  241 FNPLSSMVSL QNELSNTNRQ QQSSSMSITP IKNLLGRDAQ NFLLLGLLNK NFKGNWSLED 300
  301 PSGSVEIDIS QTIPTQGHYY VPGCMVLVEG IYYSVGNKFH VTSMTLPPGE RREITLETIG 360
  361 NLDLLGIHGI SNNNFIARLD KDLKIRLHLL EKELTDHKFV ILGANLFLDD LKIMTALSKI 420
  421 LQKLNDDPPT LLIWQGSFTS VPVFASMSSR NISSSTQFKN NFDALATLLS RFDNLTENTT 480
  481 MIFIPGPNDL WGSMVSLGAS GTLPQDPIPS AFTKKINKVC KNVVWSSNPT RIAYLSQEIV 540
  541 IFRDDLSGRF KRHRLEFPFN ESEDVYTEND NMMSKDTDIV PIDELVKEPD QLPQKVQETR 600
  601 KLVKTILDQG HLSPFLDSLR PISWDLDHTL TLCPIPSTMV LCDTTSAQFD LTYNGCKVIN 660
  661 PGSFIHNRRA RYMEYVPSSK KTIQEEIYI

    Protein Complex Data

      PUBLICATION TOPOLOGY COCOMPLEXED PROTEINS
    View Details Riffle et al. (2010) (Unpublished Data) DPB2 DPB3 DPB4 POL2
    View Details (MIPS) Mewes HW, et al. (2004) CDC45 DPB11 DPB2 DPB3 MCM2 MCM3 MCM4 MCM5 MCM6 MCM7 ORC1 ORC2 ORC3 ORC4 ORC5 ORC6 POL2 POL3 POL31 POL32
    View Details Krogan NJ, et al. (2006) DPB2 DPB3 DPB4 INM1 MBA1 MCM2 POL2 RPN4
    View Details Gavin AC, et al. (2006) DPB2 DPB4 POL2
    View Details Gavin AC, et al. (2002) CTF18 CTF8 DPB2 DPB4 ELG1 ISW1 ISW2 KAP114 MOT1 NAP1 NPL6 OLA1 POL2 PUF6 RET1 RFC1 RFC2 RFC3 RFC4 RFC5 RPA135 RPC82 RPO31 STH1 TOP1 TOP2 VPS1 VPS13 YMR181C
    View Details Ho Y, et al. (2002) ATP3 AVO1 CCT5 CLU1 DHH1 DPB2 FUN12 GAL7 GPH1 IDH1 ILV2 MET16 MKK2 NOG2 NPA3 PDX1 PHO85 PUF3 RPN12 SIS1 SLX1 SRP54 STI1 TEM1 TFC7 VMA8 YER077C YGR266W YKR051W YKU80 YLR271W YPR003C
    View Details Qiu et al. (2008) CDC45 CDC9 DPB11 DPB2 DPB3 DPB4 HCS1 MCM2 MCM3 MCM4 MCM5 MCM6 MCM7 ORC2 ORC3 ORC5 POL1 POL12 POL2 POL3 POL30 POL31 POL32 PRI2 RAD30 RFC1 RFC2

    Mass Spectrometry Data

    The following runs contain data for this protein:

      BAIT COMMENTS PUBLICATION
    View Run MLP2 #34 Asynchronous SPB prep Keck JM, et al. (2011)

    Yeast Two-Hybrid Data

    The following interactions contain this protein:

    SHOWING SINGLE HITS. [ Hide Single Hits ]

    [View our yeast two-hybrid interpretation guidelines.]

      BAIT PREY HITS PUBLICATION
    View Screen PSF1 DPB2 2 Hazbun TR, et al. (2003)
    View Screen PSF1 DPB2 2 Hazbun TR, et al. (2003)
    View Screen UBP6 DPB2 1 Unpublished Fields Lab Data
    View Screen RAD6 DPB2 1 Unpublished Fields Lab Data
    View Screen UBC7 DPB2 1 Unpublished Fields Lab Data

    Microscopy / Localization Data

    New Feature: Upload Your Own Microscopy Data

      PROTEIN(S) PUBLICATION
    View Data DPB2 Sundin BA, et al. (2004)
    View Data DPB2 Sundin BA, et al. (2004)
    View Data DPB2 Huh WK, et al. (2003)

    Protein Structure Data


    [What does the above image mean?]
    [View Top Sequence Alignments] [Show Ginzu Version Information]

    Domains predicted:

    #   Region(s) Method Confidence Match Description
    1 View Details [1..235] deduced N/A No confident structure predictions are available.
    2 View Details [236..361] MSA 1.048988 View MSA. No confident structure predictions are available.
    3 View Details [362..587] deduced N/A No confident structure predictions are available.
    4 View Details [588..689] MSA 3.045983 View MSA. No confident structure predictions are available.

    Functions predicted (by domain):

    # Gene Ontology predictions
    1 No functions predicted.
    2 No functions predicted.
    3
    Term Confidence Notes
  • transferase activity, transferring phosphorus-containing groups
    		•  3.253873361615 bayes_pls_golite062009
  • transferase activity
    		•  2.86536343515876 bayes_pls_golite062009
  • protein serine/threonine phosphatase activity
    		•  2.60559887605086 bayes_pls_golite062009
  • phosphatase activity
    		•  2.32143054924462 bayes_pls_golite062009
  • phosphoric ester hydrolase activity
    		•  2.29895042460743 bayes_pls_golite062009
  • phosphoprotein phosphatase activity
    		•  2.25451947416972 bayes_pls_golite062009
  • hydrolase activity, acting on ester bonds
    		•  1.69489536786659 bayes_pls_golite062009
  • binding
    		•  1.50157839850353 bayes_pls_golite062009
  • catalytic activity
    		•  1.30037658165549 bayes_pls_golite062009
  • structural molecule activity
    		•  0.371786873304259 bayes_pls_golite062009
  • phosphotransferase activity, alcohol group as acceptor
    		•  0.33393888611713 bayes_pls_golite062009
  • kinase activity
    		•  0.308698487135154 bayes_pls_golite062009
  • protein binding
    		•  0.30363127120976 bayes_pls_golite062009
  • protein kinase activity
    		•  0.108241336192419 bayes_pls_golite062009
  • hydrolase activity
    		•  0.070797330694107 bayes_pls_golite062009
    4 No functions predicted.


    Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076


    Philius Transmembrane Prediction:

    Protein predicted to be: GLOBULAR (No transmembrane regions or signal peptide)
    Confidence of classification: 0.96

    Source: Reynolds et al. (2008)

    YRC Informatics Platform - Version 3.0
    Created and Maintained by: Michael Riffle