Protein Overview: GCN3

General Information:
Name(s) found:	GCN3 / YKR026C [SGD]
Description(s) found: Found 30 descriptions. SHOW ALL	Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression [SGD]
Organism:	Saccharomyces cerevisiae
Length:	305 amino acids
Gene Ontology:
Cellular Component:	guanyl-nucleotide exchange factor complex [IDA] eukaryotic translation initiation factor 2B complex [IDA ][IMP][IPI]
Biological Process:	regulation of translational initiation [IDA][IGI][IMP ][IPI]
Molecular Function:	translation initiation factor activity [IDA][IMP] guanyl-nucleotide exchange factor activity [IDA] enzyme regulator activity [IGI][IMP ][IPI]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSEFNITETY LRFLEEDTEM TMPIAAIEAL VTLLRIKTPE TAAEMINTIK SSTEELIKSI  60
   61 PNSVSLRAGC DIFMRFVLRN LHLYGDWENC KQHLIENGQL FVSRAKKSRN KIAEIGVDFI 120
  121 ADDDIILVHG YSRAVFSLLN HAANKFIRFR CVVTESRPSK QGNQLYTLLE QKGIPVTLIV 180
  181 DSAVGAVIDK VDKVFVGAEG VAESGGIINL VGTYSVGVLA HNARKPFYVV TESHKFVRMF 240
  241 PLSSDDLPMA GPPLDFTRRT DDLEDALRGP TIDYTAQEYI TALITDLGVL TPSAVSEELI 300
  301 KMWYD

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		GCD1 GCD11 GCD2 GCD6 GCD7 GCN3 SUI2 SUI3
View Details	(MIPS) Mewes HW, et al. (2004)		GCD1 GCD2 GCD6 GCD7 GCN3
View Details	Krogan NJ, et al. (2006)		ADE1 GCD1 GCD11 GCD2 GCD6 GCD7 GCN3 MRF1 MRH4 SUI2
View Details	Gavin AC, et al. (2006)		GCD1 GCD11 GCD2 GCD6 GCD7 GCN3
View Details	Gavin AC, et al. (2002)		CCT4 CHC1 COG8 GCD1 GCD11 GCD2 GCD6 GCD7 GCN3 GUT1 IST1 PDC1 PRO1 RRP40 RRP46 SUI2 SUI3 XKS1
View Details	Ho Y, et al. (2002)		ABP1 ACO1 AIM41 ATP4 BGL2 BUD14 CAR2 CBP6 CDC28 CDC33 CEG1 CKA1 CKA2 CKB1 CKB2 COR1 CRZ1 CYS4 DCP1 DCP2 DNM1 EDC3 EDE1 EGD2 ENP1 FAA1 FAA4 FAL1 FET3 FHL1 FKH1 FRS2 FYV8 GAS1 GCD1 GCD11 GCD2 GCD6 GCD7 GCN3 GPH1 GUF1 HHF1, HHF2 HMO1 HRR25 HSP104 HTA1 HTB1 HXT7 HYP2 IPP1 LOC1 LOS1 LSP1 LTV1 MBP1 MCX1 MDH1 MDS3 MET18 MGE1 MGM101 MKT1 MPH1 NDE1 NET1 NMD5 NOP1 NPA3 NSA1 NUG1 OYE2 PET10 PET9 PEX19 PIL1 PIN4 PRO3 PTC4 PUF3 RIO2 RPC19 RPM2 RRP1 SAP185 SAP190 SAS10 SCW4 SEC2 SEC23 SES1 SFB3 SGM1 SIN3 SIT4 SLC1 SUI2 SUI3 TIF1, TIF2 TIF4631 TIF4632 TMA19 TRP3 TRP5 TSR1 TUP1 UBP12 URE2 VAC8 VMA4 VMA8 VPS8 YBR225W YER138C YGR017W YHM2 YMR144W
View Details	Qiu et al. (2008)		GCD1 GCD2 GCD6 GCD7 GCN3

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	BOI1	Sample boi 1 with ha tag from october 2005	McCusker D, et al (2007)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	SAM35	GCN3	1	Unpublished Fields Lab Data
View Screen	PLB1	GCN3	1	Unpublished Fields Lab Data
View Screen	SNF4	GCN3	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	GCN3	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..234]	ORFEUS	7.83	Signal sequence recognition protein Ffh; Signal sequence binding protein Ffh; GTPase domain of the signal sequence recognition protein Ffh
2	View Details	[235..305]	ORFEUS	10.02	D-ribose-5-phosphate isomerase (RpiA), catalytic domain; D-ribose-5-phosphate isomerase (RpiA), lid domain

Functions predicted (by domain):

Gene Ontology predictions

Term	Confidence	Notes
translation initiation factor activity	10.1099993541596	bayes_pls_golite062009
translation regulator activity	5.74669335554405	bayes_pls_golite062009
nucleic acid binding	2.17718456512191	bayes_pls_golite062009
transcription regulator activity	1.9283980201455	bayes_pls_golite062009
DNA binding	1.55389679363348	bayes_pls_golite062009
binding	1.53908996868286	bayes_pls_golite062009
transferase activity, transferring sulfur-containing groups	1.40526340076289	bayes_pls_golite062009
catalytic activity	1.31732715713197	bayes_pls_golite062009
translation factor activity, nucleic acid binding	1.09424756417577	bayes_pls_golite062009
transcription repressor activity	1.03828732072006	bayes_pls_golite062009
transcription factor activity	0.995059566132783	bayes_pls_golite062009
deaminase activity	0.518630450774121	bayes_pls_golite062009
S-methyl-5-thioribose-1-phosphate isomerase activity	0.511292518421556	bayes_pls_golite062009
sugar binding	0.319281682893106	bayes_pls_golite062009
protein binding	0.0293610121675921	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.98

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2