Protein Overview: MAK21

General Information:
Name(s) found:	MAK21 / YDR060W [SGD]
Description(s) found: SHOW ONLY BEST	gi\|798911\|emb\|CAA89089.1\| unknown [Saccharomyces cerevisiae] [NCBI NR] gi\|706826\|emb\|CAA58976.1\| unknown [Saccharomyces cerevisiae] [NCBI NR] gi\|6320265\|ref\|NP_010345.1\| Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein; Ma [NCBI NR] gi\|6225679\|sp\|Q12176.1\|MAK21_YEAST RecName: Full=Ribosome biogenesis protein MAK21; AltName: Full=Maintenance of killer protein 21; AltName: Full=Nucleolar complex protein 1 [NCBI NR] gi\|1431509\|emb\|CAA98878.1\| unnamed protein product [Saccharomyces cerevisiae] [NCBI NR] pir\|\|S54044 probable membrane protein YDR060w - yeast (Saccharomyces cerevisiae) [NCBI NR] (Q12176) Ribosome biogenesis protein MAK21 [Swiss-Prot] Ribosome biogenesis protein MAK21 OS=Saccharomyces cerevisiae GN=MAK21 PE=1 SV=1 [Swiss-Prot] MAK21 Protein required for 60S ribosomal subunit biogenesis [mips] Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein [SGD] YDR060W, Chr IV from 570643-573720 [yeast_orfs2] Chr IV from 570643-573720 [yeastorf3.fasta] MAK21 SGDID:S0002467, Chr IV from 570644-573721, Verified ORF [yeastflysequest.fasta] MAK21 SGDID:S0002467, Chr IV from 570645-573722, Verified ORF [10112005cervSGD.fasta] Mak21p [Saccharomyces cerevisiae]gi\|1431509\|emb\|CAA98878.1\| unnamed protein product [Saccharomyces cerevisiae]gi\|798911\|emb\|CAA89089.1\| unknown [Saccharomyces cerevisiae]gi\|706826\|emb\|CAA58976.1\| unknown [Saccharomyces cerevisiae]gi\|1077232\|pir\|\|S5404 [nrdb_11152004_Chlamydomonas] MAK21 SGDID:S000002467, Chr IV from 570645-573722, Verified ORF, "Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homo [SGD_S-cerevisiae_na_12-16-2005_con_reversed.fasta] MAK21 SGDID:S000002467, Chr IV from 570646-573723, Verified ORF, "Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homo [yeast-contam.fasta] MAK21 SGDID:S000002467, Chr IV from 570647-573724, Verified ORF, "Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homo [SGD_S-cerevisiae_na_08-19-2008_con_reversed.fasta] Ribosome biogenesis protein MAK21 (Maintenance of killer protein 21) (Nucleolar complex protein 1) [nr-271106-contam.fasta] unknown [Saccharomyces cerevisiae] [nr-271106-contam.fasta] Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein; Mak21p [Saccharomyces cerevisi [nr-271106-contam.fasta] unnamed protein product [Saccharomyces cerevisiae] [nr-271106-contam.fasta] MAK21 SGDID:S000002467, Chr IV from 570647-573724, Verified ORF, "Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein" [yeast-bovrabprots.fasta] MAK21 SGDID:S000002467, Chr IV from 570646-573723, Verified ORF, "Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein" [yeast-contam_custom.fasta] [chemostatdb.fasta] Ribosome biogenesis protein MAK21 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MAK21 PE=1 SV=1 [UniProt_S_cerevisiae_20120516_05-16-2012_reversed.fasta] MAK21 SGDID:S000002467, Chr IV from 570649-573726, Genome Release 64-1-1, Verified ORF, "Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein" [yeast-030211-contam.fasta] MAK21 SGDID:S000002467, Chr IV from 570649-573726, Genome Release 64-2-1, Verified ORF, "Constituent of 66S pre-ribosomal particles; required for large (60S) ribosomal subunit biogenesis; acts as part of a Mak21p-Noc2p-Rrp5p module that associates with nascent pre-rRNA during transcription and has a role in bigenesis of the large ribosomal subunit; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein" [20141208_orf_trans.fasta] YDR060W SGDID:S000002467, Chr IV from 570649-573726, Genome Release 64-1-1, Verified ORF, "Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein" [2016-01-SGD_Yeast_Contam_Riffle_20140507-Ska-Hec-110-tubulin.fasta]
Organism:	Saccharomyces cerevisiae
Length:	1025 amino acids
Gene Ontology:
Cellular Component:	Noc1p-Noc2p complex [IPI]
Biological Process:	ribosome biogenesis [RCA] ribosomal large subunit assembly [IMP]
Molecular Function:	molecular_function [ND]

Sequence:

Sequence:
[PDR BLAST]
[ProtParam]

      1          11         21         31         41         51         
      |          |          |          |          |          |          
    1 MSENNGNPLD LSSLRNKISS KLRDNNSKKA KKTHKGKDVK ASSNSKKVNE DIRREALALG  60
   61 ASEEDLKLIQ GLSDDDDAKS EQEFDAVADE DADDKGFKND LQNFMKNVGF DQHKLEDVDD 120
  121 DDIEEESTSS KESKIPAQEK EHAQSNIASS TIEKTSQESI DNGSEQEENT VEEANLSSDQ 180
  181 EPESESAEKE KKEEKDGGLI TQTTIISSDK LIIPYDKPWY EIPLDPQVGQ NDDVEELSKE 240
  241 QIEKLFERGK QTLEADNQTY YEEFTKDSSQ AKFMSQILSD GTLNDKISAV TLLIQDSPLH 300
  301 NTKSLETLVS YCGKKSRNSA LQSLNALKDL FLNGLLPNRK LRYFKNQPGL SMMLNKKTLA 360
  361 IFYFEDYLKK LFFRVLEVLE VLSHDPIIHV RLQILNHVFD LLTNQPEQEF NLLRLGVNKI 420
  421 GDIDSKVSSK ASYLLLKLEQ AHPNMKSIVI DAIVDIALRP NADYHTTYYS VITLNQTILK 480
  481 RSEDSVANKL VKTYFTLFEK FLINTDKDNT NGVVKSNSKS YEEKRKKNFK KGKHGGKSVK 540
  541 IEKTENEVLD EKNSKLFSAL LTGINRAFPF AQIPASVYEV HMETLFKITH SSNFNTSIQA 600
  601 LVLINQVTVK AKLNSDRYYR TLYESLFDPR LVNSSKQGIY LNLLYKSLKQ DALNVERVEA 660
  661 FVKRILQVCS HWLNVGTITG FFFLLIQLAK TVPQIKNLLT NTPVDYEYES DAEEEQGDKD 720
  721 IKRKEYDGRK RDPKFANAEK SSLWEINNFI NHFHPTVKTY ANAYVTGETE QIAKPDLGLF 780
  781 TLSHFLDRFV YRSAKQTNTA RGTSIMQPLF SGSRVNDSVL VKASDIMHDQ GPVNTEDWLT 840
  841 KKVEDIKPED KFFYQYFTTK KTADGKGKKS NKASNFDSDD EMDENEIWSA LVKSRPDVED 900
  901 DSDDSELDFA EDDFSDSTSD DEPKLDAIDD EDAKSEGSQE SDQEEGLDED IFYSFDGEQD 960
  961 NSDKKRSFAE SSEEDESSEE EKEEEENKEV SAKRAKKKQR KNMLKSLPVF ASADDYAQYL1020
 1021 DQDSD

Protein Complex Data

	PUBLICATION	TOPOLOGY	COCOMPLEXED PROTEINS
View Details	Riffle et al. (2010) (Unpublished Data)		ATP22 BRX1 CIC1 DBP10 DBP9 DRS1 EBP2 ERB1 FPR4 HAS1 HCA4 MAK21 MAK5 NIP7 NOC2 NOC3 NOG1 NOP12 NOP15 NOP2 NOP4 NOP7 NSA1 NUG1 PUF6 RIX7 RLP7 RPF1 RPF2 RPL15A RPL18A, RPL18B RPL5 RPS4B, RPS4A RRP12 RRP5 SPB1 SPB4 TIF6 URB1 UTP22 YTM1
View Details	Krogan NJ, et al. (2006)		BUD22 CIC1 DBP6 EFG1 FRE3 GIS2 HAS1 MAK21 NIP7 NOB1 NOC2 NOC3 NOP1 NOP2 NOP8 PUS7 RRP5 RSA3 SBP1 SFA1 SSQ1 YGR272C YMR034C YRF1-8, YRF1-5, ...
View Details	Gavin AC, et al. (2006)		BRX1 CIC1 DBP9 ERB1 MAK21 MAK5 NOC2 NOP4 PUF6 RLP7 RSA3 SPB4 YTM1
View Details	Gavin AC, et al. (2002)		AAR2 ARX1 BEM2 BMS1 BRX1 BUD20 CBF5 CIC1 CLU1 CYR1 CYS4 DBP10 DBP9 DCP2 DRS1 EBP2 ERB1 FPR4 FUN12 GAR1 GCN1 HAS1 HDA1 HER1 HIS3 INP2 IPI1 IPI3 KRE33 KRR1 LHP1 LOC1 LSG1 MAK21 MAK5 MDN1 MIS1 MRP7 MRT4 NAP1 NHP2 NIP1 NIP7 NMD3 NOC2 NOC3 NOG1 NOG2 NOP1 NOP12 NOP15 NOP16 NOP2 NOP4 NOP56 NOP6 NOP7 NSA1 NSA2 NUG1 POL2 PRP4 PRT1 PUF6 REI1 RIX1 RIX7 RLP24 RLP7 RPA135 RPA190 RPF1 RPF2 RPG1 RPP2B RPT6 RRP1 RRP12 RSA3 RSA4 RTR1 SDA1 SEC7 SKI2 SMB1 SNU114 SNU13 SNU66 SPB1 SPB4 SRO9 SSF1 TIF4631 TIF4632 TIF6 TUB3 URB1 UTP10 UTP20 UTP21 UTP22 YGL036W YMR310C YTM1
View Details	Qiu et al. (2008)		DBP6 MAK21 MAK5 NOC2 RSA3

Mass Spectrometry Data

The following runs contain data for this protein:

	BAIT	COMMENTS	PUBLICATION
View Run	MLP2	#32 Asynchronous Prep-No Phosphopeptide enrichment	Keck JM, et al. (2011)
View Run	MLP2	#31 Asynchronous Prep (Protease cleavage)	Keck JM, et al. (2011)

Yeast Two-Hybrid Data

The following interactions contain this protein:

SHOWING SINGLE HITS. [ Hide Single Hits ]

[View our yeast two-hybrid interpretation guidelines.]

	BAIT	PREY	HITS	PUBLICATION
View Screen	PSF2	MAK21	1	Hazbun TR, et al. (2003)
View Screen	PSF2	MAK21	1	Hazbun TR, et al. (2003)
View Screen	SPT21	MAK21	1	Unpublished Fields Lab Data

Microscopy / Localization Data

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	PROTEIN(S)	PUBLICATION
View Data	MAK21	Huh WK, et al. (2003)
View Data	MAK21 and NOP56	Huh WK, et al. (2003)

Protein Structure Data

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[View Top Sequence Alignments] [Show Ginzu Version Information]

Domains predicted:

#		Region(s)	Method	Confidence	Match Description
1	View Details	[1..213]	deduced	N/A	No confident structure predictions are available.
2	View Details	[214..922]	Pfam	1000.0	CBF/Mak21 family No confident structure predictions are available.
3	View Details	[923..1025]	deduced	N/A	No confident structure predictions are available.

Functions predicted (by domain):

Gene Ontology predictions

No functions predicted.

Term	Confidence	Notes
binding	2.81296846555705	bayes_pls_golite062009
RNA binding	2.62914297393378	bayes_pls_golite062009
structural molecule activity	2.41912877876968	bayes_pls_golite062009
protein binding	1.81870125631028	bayes_pls_golite062009
mRNA binding	0.868658457661827	bayes_pls_golite062009
cytoskeletal protein binding	0.19418057335311	bayes_pls_golite062009
nucleic acid binding	0.118461769548431	bayes_pls_golite062009

No functions predicted.

Reference: Malmström L, et al. (2007) Superfamily assignments for the yeast proteome through integration of structure prediction with the gene ontology. PLoS Biol 5(4): e76. doi:10.1371/journal.pbio.0050076

Philius Transmembrane Prediction:

Protein predicted to be:	GLOBULAR (No transmembrane regions or signal peptide)
Confidence of classification:	0.93

Source: Reynolds et al. (2008)

TMHMM	2.0c	PSIPRED	v2.61
SignalP	3.0	COILS	1999
DISOPRED	v0.1	NCBI nr	06/2007
PSI-BLAST	2.2.18	FFAS	ffas03
PDB	06/2007	Pfam	22
HMMER	2.3.2